FvH4_2g03391
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007480
Physical Location & Seq
Reverse (-)
2710041 .. 2712436
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03391.t1

Sequence Viewer

Length: 1539 bp
ATGGGAGCCGTTCTGTTACAAGATGGGAAGCCAGTAGAATACTACTCTGAGTTATTCACTGGATCCATCAGAAACTATCCAACCTACGACAAGGAGTTTTATGCACTTTACCAAGCCATCAAGCATTGGAGGGTGTACTTGTTAGGGAAGGAGGTAGTAGTACATTCAGATCACAAACCATTGGAATATCTTCATGCTCAAACCAAGCTACAACAAGCCAGGCACATGAAGTGGATGTCATATTTAATGACTTTTGACATAGTCATCCGATACAAGAAGGGGATCACCAACAAGTTGGCTGATATGTTGTCAAGACCACCTGTGAGAGTTTGTGCCTTGGTAGTGGCTATGCGTATACAACCTTTGGTTCCAACTGAGTACGCAACTTTGTATGAATCAGATAAAGGTTTCAAGGAAGTATATGAGCAAGCAAAACGAGTGCAGAAAGGGGAGTTTGAGATACGAGATGGTTTGTTGTATAAGGGATCATTGTTATGCATACCGGAAGAGGGAGATCGATTACAGTGGATTCGAGAAGCACATACTTCAAGAGTGGCTAGACACTTTGGAGTTAATAAGACTTTGTTGAATTTACGGCGTTATGTCTTCTGGCCTAAGATGCAGGATGATGTTTCCAGATTTGTTCGAGGGTGTAAGTTGTGTTGTACTTCGAAACCAGCGAACAGGAAGAGAGGGTTGTATCTACCGTTGCCGGTACCGTCTCGTCCTTGGGAAAGTATTTCCATGGATTTCTTGGGAGGTTTACCGAAGGCCAAGTCTGGAAATGACTATTTATATGTTGTGGTCGATCGTTTTAGTAAAATGGTGGTTCTCATTCCTTGCAAGAAGACCATTACAGGGGCTGGTGCCGCTAAGTTATTCTTTGAGAATGTCTGGAAGCATTTTGGGTTGCCTAGTTCGATTATTTCTGATCGTGACAGCAGGTTTCTTGGTCACTTTTGGGATTCTTTGTGGGGAATGATGGATACACGCCTGAAAAGAAGTACAGCCTTTCATCCACAAACGGATGGCCAGACTGAAGTAGTTAACAGAACTATGGTGCATCTATTGCGGGGATACAATGCTAAGCATCCGAAGACTTGGGATGAGAGTCTTCCGTATTTACAGTTTGCTTTCAACAGGGCTGTTCATAGTTCTTCTGGGACATCTCCATTTGAAGCTTGTTATGGCTACTTACCACCAAGTCCGTTTGATATGGTTTTTACTGTAGGTGAAGGTAAGAAGTTGAAGCCACTTCGGTACGGTCCTTTCCGTATTCTGCAGCAGATTGGCGACAATGCCTTCAAACTCGACTTGCCGCCATATTTGGGCATGTATTCAGTTATCAATGCTGAGTACTTAAAGTTGTTTGAACCACCATTGCTTGATGATGATGGGGATGACAAGGTTGTTTTACCACCAGTTGATGACTTATGCGATGGAACTGGAAGGGATGGCGGTGATCTCGCAGTAGGAGGCAGTAGCTCCGATGTCGTTACTACTTCTGAGAACCATGCCAAGTCTGTTTTTCGCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

513

Amino Acids

58.48

Weight (kDa)

9.12

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH PF17917 1 - 85 5.2e-23 RNase H-like domain found in reverse transcriptase
RT_RNaseH_2 PF17919 1 - 54 9.5e-12 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 172 - 226 6.7e-16 Integrase zinc binding domain
rve PF00665 241 - 335 1.3e-07 Integrase core domain
SH3_Tf2-1 PF24626 410 - 456 3e-10 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 933
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 2 cut(s) 715, 866
AccI GTMKAC 1 cut(s) 355
AciI CCGC 4 cut(s) 870, 1072, 1319, 1458
AclWI GGATC 4 cut(s) 57, 70, 290, 493
AcoI YGGCCR 1 cut(s) 1030
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 1059
AfaI GTAC 8 cut(s) 137, 162, 380, 667, 717, 1006, 1262, 1358
AfiI CCNNNNNNNGG 3 cut(s) 509, 858, 1328
AgsI TTSAA 8 cut(s) 412, 549, 589, 1138, 1178, 1249, 1306, 1373
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 3 cut(s) 208, 1181, 1485
AluI AGCT 3 cut(s) 208, 1181, 1485
Alw26I GTCTC 1 cut(s) 726
AlwI GGATC 4 cut(s) 57, 70, 290, 493
AlwNI CAGNNNCTG 1 cut(s) 863
AoxI GGCC 3 cut(s) 611, 771, 1030
ApeKI GCWGC 1 cut(s) 1282
ApoI RAATTY 1 cut(s) 589
ArsI GACNNNNNNTTYG 2 cut(s) 761, 793
Asp700I GAANNNNTTC 1 cut(s) 189
Asp718I GGTACC 1 cut(s) 715
AspS9I GGNCC 1 cut(s) 1265
AsuHPI GGTGA 3 cut(s) 277, 1244, 1472
AsuII TTCGAA 1 cut(s) 671
AvaII GGWCC 1 cut(s) 1265
BalI TGGCCA 1 cut(s) 1032
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 2 cut(s) 715, 866
BbsI GAAGAC 4 cut(s) 598, 854, 1103, 1106
BbvI GCAGC 1 cut(s) 1294
BccI CCATC 9 cut(s) 17, 74, 125, 461, 976, 1022, 1388, 1433, 1448
BceAI ACGGC 1 cut(s) 611
BciT130I CCWGG 1 cut(s) 220
BciVI GTATCC 2 cut(s) 979, 1070
BcoDI GTCTC 1 cut(s) 726
BfaI CTAG 2 cut(s) 558, 915
BfmI CTRYAG 2 cut(s) 1227, 1280
BfuAI ACCTGC 1 cut(s) 933
BfuI GTATCC 2 cut(s) 979, 1070
BisI GCNGC 3 cut(s) 870, 1283, 1319
BlpI GCTNAGC 1 cut(s) 1086
BlsI GCNGC 3 cut(s) 871, 1284, 1320
BmcAI AGTACT 1 cut(s) 1358
Bme1390I CCNGG 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 1265
BmgT120I GGNCC 1 cut(s) 1265
BmiI GGNNCC 5 cut(s) 7, 64, 369, 717, 868
BmrFI CCNGG 1 cut(s) 220
BmsI GCATC 3 cut(s) 609, 1072, 1099
BpiI GAAGAC 4 cut(s) 598, 854, 1103, 1106
Bpu1102I GCTNAGC 1 cut(s) 1086
Bpu14I TTCGAA 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 517
BsaJI CCNNGG 3 cut(s) 336, 728, 744
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 3 cut(s) 509, 858, 1328
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 4 cut(s) 32, 64, 1421, 1450
Bse3DI GCAATG 1 cut(s) 1379
BseBI CCWGG 1 cut(s) 220
BseCI ATCGAT 1 cut(s) 517
BseDI CCNNGG 3 cut(s) 336, 728, 744
BseGI GGATG 9 cut(s) 240, 264, 631, 1015, 1033, 1090, 1111, 1405, 1459
BseLI CCNNNNNNNGG 3 cut(s) 509, 858, 1328
BseMI GCAATG 1 cut(s) 1379
BseMII CTCAG 4 cut(s) 39, 366, 1344, 1497
BseNI ACTGG 4 cut(s) 32, 64, 1421, 1450
BseXI GCAGC 1 cut(s) 1294
BsgI GTGCAG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 3 cut(s) 613, 773, 1032
BshNI GGYRCC 2 cut(s) 715, 866
BshVI ATCGAT 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 811
BsiSI CCGG 2 cut(s) 503, 713
BslFI GGGAC 1 cut(s) 1177
BslI CCNNNNNNNGG 3 cut(s) 509, 858, 1328
BsmAI GTCTC 1 cut(s) 726
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 1 cut(s) 1177
BsnI GGCC 3 cut(s) 613, 773, 1032
Bsp119I TTCGAA 1 cut(s) 671
Bsp143I GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
Bsp1720I GCTNAGC 1 cut(s) 1086
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 4 cut(s) 870, 1072, 1319, 1458
BspANI GGCC 3 cut(s) 613, 773, 1032
BspCNI CTCAG 4 cut(s) 40, 367, 1345, 1498
BspDI ATCGAT 1 cut(s) 517
BspLI GGNNCC 5 cut(s) 7, 64, 369, 717, 868
BspMAI CTGCAG 1 cut(s) 1284
BspMI ACCTGC 1 cut(s) 933
BspPI GGATC 4 cut(s) 57, 70, 290, 493
BspT104I TTCGAA 1 cut(s) 671
BspT107I GGYRCC 2 cut(s) 715, 866
BsrDI GCAATG 1 cut(s) 1379
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 4 cut(s) 32, 64, 1421, 1450
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 336, 728, 744
BssMI GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
BssNAI GTATAC 1 cut(s) 356
BssT1I CCWWGG 3 cut(s) 336, 728, 744
Bst1107I GTATAC 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 6 cut(s) 525, 708, 720, 1128, 1228, 1265
Bst6I CTCTTC 2 cut(s) 501, 683
BstAPI GCANNNNNTGC 1 cut(s) 1069
BstBI TTCGAA 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 7 cut(s) 48, 375, 615, 873, 1086, 1353, 1506
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 9 cut(s) 240, 264, 631, 1015, 1033, 1090, 1111, 1405, 1459
BstKTI GATC 8 cut(s) 65, 172, 285, 488, 517, 811, 934, 1465
BstMAI GTCTC 1 cut(s) 726
BstMBI GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 3 cut(s) 619, 869, 1069
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 1 cut(s) 1336
BstSCI CCNGG 1 cut(s) 218
BstSFI CTRYAG 2 cut(s) 1227, 1280
BstV1I GCAGC 1 cut(s) 1294
BstV2I GAAGAC 4 cut(s) 598, 854, 1103, 1106
BstX2I RGATCY 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 62
BstZ17I GTATAC 1 cut(s) 356
Bsu15I ATCGAT 1 cut(s) 517
BsuI GTATCC 2 cut(s) 979, 1070
BsuRI GGCC 3 cut(s) 613, 773, 1032
BsuTUI ATCGAT 1 cut(s) 517
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 1452
BtsCI GGATG 9 cut(s) 240, 264, 631, 1015, 1033, 1090, 1111, 1405, 1459
BtsIMutI CAGTG 2 cut(s) 57, 530
BveI ACCTGC 1 cut(s) 933
Cac8I GCNNGC 1 cut(s) 429
CaiI CAGNNNCTG 1 cut(s) 863
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 1265
ClaI ATCGAT 1 cut(s) 517
Csp6I GTAC 8 cut(s) 136, 161, 379, 666, 716, 1005, 1261, 1357
CviAII CATG 5 cut(s) 194, 226, 745, 1333, 1514
CviQI GTAC 8 cut(s) 136, 161, 379, 666, 716, 1005, 1261, 1357
DdeI CTNAG 7 cut(s) 48, 375, 615, 873, 1086, 1353, 1506
DpnI GATC 8 cut(s) 64, 171, 284, 487, 516, 810, 933, 1464
DpnII GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
EaeI YGGCCR 1 cut(s) 1030
Eam1104I CTCTTC 2 cut(s) 501, 683
EarI CTCTTC 2 cut(s) 501, 683
Eco130I CCWWGG 3 cut(s) 336, 728, 744
Eco47I GGWCC 1 cut(s) 1265
Eco57I CTGAAG 1 cut(s) 1059
EcoRII CCWGG 1 cut(s) 218
EcoT14I CCWWGG 3 cut(s) 336, 728, 744
EcoT22I ATGCAT 1 cut(s) 500
ErhI CCWWGG 3 cut(s) 336, 728, 744
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 5 cut(s) 197, 229, 748, 1336, 1517
FalI AAGNNNNNCTT 2 cut(s) 866, 898
FaqI GGGAC 1 cut(s) 1177
FatI CATG 5 cut(s) 193, 225, 744, 1332, 1513
FauI CCCGC 1 cut(s) 1065
FblI GTMKAC 1 cut(s) 355
Fnu4HI GCNGC 3 cut(s) 870, 1283, 1319
FokI GGATG 9 cut(s) 247, 251, 638, 1002, 1040, 1077, 1118, 1412, 1466
Fsp4HI GCNGC 3 cut(s) 870, 1283, 1319
FspBI CTAG 2 cut(s) 558, 915
GluI GCNGC 3 cut(s) 870, 1283, 1319
HaeIII GGCC 3 cut(s) 613, 773, 1032
HapII CCGG 2 cut(s) 503, 713
Hin1II CATG 5 cut(s) 197, 229, 748, 1336, 1517
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 1179
HinfI GANTC 4 cut(s) 395, 529, 965, 1111
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 2 cut(s) 503, 713
HphI GGTGA 3 cut(s) 277, 1244, 1472
Hpy166II GTNNAC 4 cut(s) 136, 356, 764, 1048
Hpy188I TCNGA 9 cut(s) 49, 71, 169, 269, 400, 931, 1095, 1489, 1507
Hpy188III TCNNGA 7 cut(s) 312, 533, 549, 636, 780, 895, 935
Hpy8I GTNNAC 4 cut(s) 136, 356, 764, 1048
HpyAV CCTTC 6 cut(s) 142, 271, 763, 1229, 1312, 1443
HpyCH4III ACNGT 6 cut(s) 525, 708, 720, 1128, 1228, 1265
HpyCH4V TGCA 7 cut(s) 104, 442, 498, 622, 843, 1063, 1282
HpyF10VI GCNNNNNNNGC 3 cut(s) 619, 869, 1069
HpyF3I CTNAG 7 cut(s) 48, 375, 615, 873, 1086, 1353, 1506
Hsp92II CATG 5 cut(s) 197, 229, 748, 1336, 1517
KpnI GGTACC 1 cut(s) 719
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
LmnI GCTCC 2 cut(s) 5, 1490
Lsp1109I GCAGC 1 cut(s) 1294
LweI GCATC 3 cut(s) 609, 1072, 1099
MaeI CTAG 2 cut(s) 558, 915
MaeIII GTNAC 4 cut(s) 15, 935, 953, 1495
MalI GATC 8 cut(s) 64, 171, 284, 487, 516, 810, 933, 1464
MboI GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
MboII GAAGA 8 cut(s) 182, 518, 598, 700, 859, 1106, 1108, 1149
MflI RGATCY 1 cut(s) 62
MlsI TGGCCA 1 cut(s) 1032
MluCI AATT 1 cut(s) 589
MluNI TGGCCA 1 cut(s) 1032
MlyI GAGTC 1 cut(s) 1120
MmeI TCCRAC 2 cut(s) 104, 395
MnlI CCTC 7 cut(s) 123, 145, 502, 641, 686, 752, 1469
Mox20I TGGCCA 1 cut(s) 1032
Mph1103I ATGCAT 1 cut(s) 500
MroXI GAANNNNTTC 1 cut(s) 189
MscI TGGCCA 1 cut(s) 1032
MseI TTAA 4 cut(s) 245, 573, 1047, 1361
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 1 cut(s) 1032
MspI CCGG 2 cut(s) 503, 713
MspR9I CCNGG 1 cut(s) 220
MvaI CCWGG 1 cut(s) 220
MwoI GCNNNNNNNGC 3 cut(s) 619, 869, 1069
NcoI CCATGG 1 cut(s) 744
NdeII GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
NlaIII CATG 5 cut(s) 197, 229, 748, 1336, 1517
NlaIV GGNNCC 5 cut(s) 7, 64, 369, 717, 868
NmuCI GTSAC 2 cut(s) 935, 953
NsiI ATGCAT 1 cut(s) 500
NspI RCATGY 1 cut(s) 1336
NspV TTCGAA 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 3 cut(s) 395, 529, 965
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 871, 1284, 1320
Ple19I CGATCG 1 cut(s) 811
PleI GAGTC 1 cut(s) 1119
PpsI GAGTC 1 cut(s) 1119
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 5 cut(s) 7, 64, 369, 717, 868
PspPI GGNCC 1 cut(s) 1265
PstI CTGCAG 1 cut(s) 1284
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 1 cut(s) 62
PsyI GACNNNGTC 1 cut(s) 260
PvuI CGATCG 1 cut(s) 811
RsaI GTAC 8 cut(s) 137, 162, 380, 667, 717, 1006, 1262, 1358
RsaNI GTAC 8 cut(s) 136, 161, 379, 666, 716, 1005, 1261, 1357
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 4 cut(s) 245, 573, 1047, 1361
SatI GCNGC 3 cut(s) 870, 1283, 1319
Sau3AI GATC 8 cut(s) 62, 169, 282, 485, 514, 808, 931, 1462
Sau96I GGNCC 1 cut(s) 1265
ScaI AGTACT 1 cut(s) 1358
SchI GAGTC 1 cut(s) 1120
ScrFI CCNGG 1 cut(s) 220
SfaNI GCATC 3 cut(s) 609, 1072, 1099
SfcI CTRYAG 2 cut(s) 1227, 1280
SfuI TTCGAA 1 cut(s) 671
SinI GGWCC 1 cut(s) 1265
SmiMI CAYNNNNRTG 1 cut(s) 493
Sse9I AATT 1 cut(s) 589
SsiI CCGC 4 cut(s) 870, 1072, 1319, 1458
SspMI CTAG 2 cut(s) 558, 915
StyD4I CCNGG 1 cut(s) 218
StyI CCWWGG 3 cut(s) 336, 728, 744
TaaI ACNGT 6 cut(s) 525, 708, 720, 1128, 1228, 1265
TaqI TCGA 7 cut(s) 517, 532, 646, 671, 807, 920, 1311
TasI AATT 1 cut(s) 589
TatI WGTACW 5 cut(s) 135, 160, 665, 1004, 1356
TauI GCSGC 2 cut(s) 872, 1321
TfiI GAWTC 3 cut(s) 395, 529, 965
Tru1I TTAA 4 cut(s) 245, 573, 1047, 1361
Tru9I TTAA 4 cut(s) 245, 573, 1047, 1361
TscAI CASTG 2 cut(s) 64, 530
TseFI GTSAC 2 cut(s) 935, 953
TseI GCWGC 1 cut(s) 1282
Tsp45I GTSAC 2 cut(s) 935, 953
TspDTI ATGAA 5 cut(s) 182, 242, 408, 1004, 1139
TspGWI ACGGA 4 cut(s) 1040, 1107, 1197, 1262
TspRI CASTG 2 cut(s) 64, 530
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 1265
XapI RAATTY 1 cut(s) 589
XceI RCATGY 1 cut(s) 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XmiI GTMKAC 1 cut(s) 355
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 2 cut(s) 558, 915
ZrmI AGTACT 1 cut(s) 1358
Zsp2I ATGCAT 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.