FvH4_2g03523

Serine carboxypeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002263
Physical Location & Seq
Forward (+)
2850758 .. 2863234
12477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03523.t13

Sequence Viewer

Length: 1242 bp
ATGTTCTACTACTTCATCGAGTCAGAAGGAAACCCCAAAGAAGACCCTTTGTTGCTGTGGTACAGTGGAGGCCCTGGTTGTTCTGCTTTCAATGGTCTCATTTACGAAAACGGTCCTTTGGCTTTCAACCTCACAGATTATGAAGGCGGTGTACCAAAGACATTTTACTACCCATACTCATGGTCAAGGACTGCAAGCCTATTGTTTGTGGATGCACCTGTTGGCACTGGTTTCTCCTATGCAACTGATGCATCAGAATACGCTACTTCGGATACTAAATCAGCAGCACAGGTTTACGGGTTCTTAAGAACGTGGTTGAATGAGCACCCACAATACCTCAAGGTTCAGTTGTTCGTTGGGGCTGATTCGTATGCAGGCGTATCTGCAACCATTGTTGTTCAACATATAATGGCTGGTAATGATGCCGGATTTAAGCCTCGCCTGAATCTCAAGGGCTATATCCTAGGGTGTCCAAAAACAGATGCAGATATCAATGAGAATTCCAAGTACAGCTATTCTCACCGGATGGGACTTATATCAGATGAACTCTATTATTCGGCTAAAAACAGCTGTAATTCAGATTTCTATGGAGCATCTGTGGATGAACCAGAATGTTATGAGGATATGCAATTGATTGCAAGGCAAATTAAAGACATAAACAAAAACCATATTCTGGAGCCAAAATGTACATGGGCTTCACCAGTACCTGATGGAGATTCAGCTCGTAGAACTATGCAGGAATACGAATACCCAGAGGACTTCCTCCGGTCACCTCTACAAAATCCTGAATTCTGTCATAACTTTAACTATGCGCTATCCTACGTATGGGCGAACGATGATGATGTTCGAGCTGCACTGAATATCAGAAATGGAACAGTATTTGATTGGAAGAGATGCAATAAGAGCTTGGATTACACATATGATGTGTCCAGTGTCCTTGAATATCATCAAAATCTCAGTACCAAGGGCCTGCAAGTTCTGATATTCAATGGTGATCATGACCTGACAATTCCGAACACCGGCACTGAAGAGTGGATAAAAAAATTGGACTTGACTGTTGTTAATGACTGGAGACCATGGCTAGTTGATGGGCAGATTGCAGGATACACCATCAAGTACTCAGAAAATGGATACCGCTTGACATATGCAACCTTAAAGGGGGCAGGTCACTCACCTCAAGAATACAAGCGTAGAGAAGGCTATGACATGTTTGATAGGTTCATCCATTACTATCCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

414

Amino Acids

47.16

Weight (kDa)

4.96

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 1 - 409 1e-104 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1154
AccB7I CCANNNNNTGG 1 cut(s) 673
AciI CCGC 2 cut(s) 147, 1135
AcsI RAATTY 2 cut(s) 499, 788
AcuI CTGAAG 1 cut(s) 1047
AfaI GTAC 7 cut(s) 62, 153, 509, 688, 705, 961, 1118
AfiI CCNNNNNNNGG 4 cut(s) 673, 825, 1019, 1158
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 1206
AgsI TTSAA 6 cut(s) 91, 127, 319, 401, 941, 988
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 5 cut(s) 513, 570, 722, 851, 906
AluI AGCT 5 cut(s) 513, 570, 722, 851, 906
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 101, 1066
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 2 cut(s) 70, 967
ApeKI GCWGC 2 cut(s) 284, 851
ApoI RAATTY 2 cut(s) 499, 788
AspA2I CCTAGG 1 cut(s) 463
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 71, 113, 967
AsuHPI GGTGA 5 cut(s) 512, 690, 762, 1004, 1164
AvaII GGWCC 1 cut(s) 113
AvrII CCTAGG 1 cut(s) 463
BarI GAAGNNNNNNTAC 4 cut(s) 135, 167, 679, 711
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 296, 838
BccI CCATC 4 cut(s) 520, 704, 1082, 1118
BciT130I CCWGG 1 cut(s) 75
BciVI GTATCC 3 cut(s) 265, 1097, 1124
BclI TGATCA 1 cut(s) 994
BcoDI GTCTC 2 cut(s) 101, 1066
BfaI CTAG 2 cut(s) 464, 1082
BfrI CTTAAG 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 1154
BfuI GTATCC 3 cut(s) 265, 1097, 1124
BisI GCNGC 2 cut(s) 285, 852
BlnI CCTAGG 1 cut(s) 463
BlsI GCNGC 2 cut(s) 286, 853
BmcAI AGTACT 1 cut(s) 1118
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 3 cut(s) 71, 113, 967
BmiI GGNNCC 1 cut(s) 678
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 7 cut(s) 202, 238, 260, 412, 472, 602, 884
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 2 cut(s) 695, 1090
BpuEI CTTGAG 3 cut(s) 323, 434, 1161
BsaAI YACGTR 1 cut(s) 823
BsaI GGTCTC 2 cut(s) 101, 1066
BsaJI CCNNGG 4 cut(s) 73, 463, 963, 1076
BsaWI WCCGGW 2 cut(s) 522, 765
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 4 cut(s) 673, 825, 1019, 1158
Bse118I RCCGGY 1 cut(s) 1019
Bse1I ACTGG 4 cut(s) 232, 701, 930, 1073
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 4 cut(s) 73, 463, 963, 1076
BseGI GGATG 4 cut(s) 217, 531, 607, 1221
BseLI CCNNNNNNNGG 4 cut(s) 673, 825, 1019, 1158
BseMII CTCAG 2 cut(s) 970, 1134
BseNI ACTGG 4 cut(s) 232, 701, 930, 1073
BseXI GCAGC 2 cut(s) 296, 838
BsgI GTGCAG 1 cut(s) 837
BshFI GGCC 2 cut(s) 72, 969
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 4 cut(s) 426, 523, 766, 1020
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 4 cut(s) 673, 825, 1019, 1158
BsmAI GTCTC 2 cut(s) 101, 1066
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 2 cut(s) 72, 969
Bso31I GGTCTC 2 cut(s) 101, 1066
Bsp1286I GDGCHC 1 cut(s) 327
Bsp1407I TGTACA 1 cut(s) 686
Bsp143I GATC 1 cut(s) 994
Bsp19I CCATGG 1 cut(s) 1076
BspACI CCGC 2 cut(s) 147, 1135
BspANI GGCC 2 cut(s) 72, 969
BspCNI CTCAG 2 cut(s) 969, 1133
BspHI TCATGA 1 cut(s) 997
BspLI GGNNCC 1 cut(s) 678
BspMI ACCTGC 1 cut(s) 1154
BspTI CTTAAG 1 cut(s) 304
BspTNI GGTCTC 2 cut(s) 101, 1066
BsrFI RCCGGY 1 cut(s) 1019
BsrGI TGTACA 1 cut(s) 686
BsrI ACTGG 4 cut(s) 232, 701, 930, 1073
BssAI RCCGGY 1 cut(s) 1019
BssECI CCNNGG 4 cut(s) 73, 463, 963, 1076
BssMI GATC 1 cut(s) 994
BssT1I CCWWGG 3 cut(s) 463, 963, 1076
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 4 cut(s) 65, 113, 877, 1057
Bst6I CTCTTC 2 cut(s) 884, 1023
BstAFI CTTAAG 1 cut(s) 304
BstAPI GCANNNNNTGC 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 686
BstBAI YACGTR 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 196, 376, 971
BstDEI CTNAG 2 cut(s) 956, 1120
BstDSI CCRYGG 1 cut(s) 1076
BstEII GGTNACC 1 cut(s) 768
BstF5I GGATG 4 cut(s) 217, 531, 607, 1221
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 1 cut(s) 997
BstMAI GTCTC 2 cut(s) 101, 1066
BstMBI GATC 1 cut(s) 994
BstMWI GCNNNNNNNGC 2 cut(s) 248, 903
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 1210
BstPI GGTNACC 1 cut(s) 768
BstSCI CCNGG 1 cut(s) 73
BstSNI TACGTA 1 cut(s) 823
BstV1I GCAGC 2 cut(s) 296, 838
BstV2I GAAGAC 1 cut(s) 48
BstXI CCANNNNNNTGG 1 cut(s) 180
BsuI GTATCC 3 cut(s) 265, 1097, 1124
BsuRI GGCC 2 cut(s) 72, 969
BtgI CCRYGG 1 cut(s) 1076
BtsCI GGATG 4 cut(s) 217, 531, 607, 1221
BtsIMutI CAGTG 5 cut(s) 70, 225, 854, 937, 1023
BveI ACCTGC 1 cut(s) 1154
Cac8I GCNNGC 3 cut(s) 196, 376, 971
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 1 cut(s) 997
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 1019
Cfr13I GGNCC 3 cut(s) 71, 113, 967
Csp6I GTAC 7 cut(s) 61, 152, 508, 687, 704, 960, 1117
CviAII CATG 5 cut(s) 180, 690, 998, 1077, 1207
CviQI GTAC 7 cut(s) 61, 152, 508, 687, 704, 960, 1117
DdeI CTNAG 2 cut(s) 956, 1120
DpnI GATC 1 cut(s) 996
DpnII GATC 1 cut(s) 994
Eam1104I CTCTTC 2 cut(s) 884, 1023
EarI CTCTTC 2 cut(s) 884, 1023
Eco105I TACGTA 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 463, 963, 1076
Eco31I GGTCTC 2 cut(s) 101, 1066
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 1047
Eco91I GGTNACC 1 cut(s) 768
EcoO109I RGGNCCY 2 cut(s) 71, 967
EcoO65I GGTNACC 1 cut(s) 768
EcoRI GAATTC 2 cut(s) 499, 788
EcoRII CCWGG 1 cut(s) 73
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 3 cut(s) 463, 963, 1076
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 463, 963, 1076
FaeI CATG 5 cut(s) 183, 693, 1001, 1080, 1210
FaqI GGGAC 1 cut(s) 543
FatI CATG 5 cut(s) 179, 689, 997, 1076, 1206
FauNDI CATATG 2 cut(s) 919, 1144
FbaI TGATCA 1 cut(s) 994
Fnu4HI GCNGC 2 cut(s) 285, 852
FokI GGATG 4 cut(s) 224, 538, 614, 1208
Fsp4HI GCNGC 2 cut(s) 285, 852
FspBI CTAG 2 cut(s) 464, 1082
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 285, 852
GsuI CTGGAG 2 cut(s) 695, 1090
HaeIII GGCC 2 cut(s) 72, 969
HapII CCGG 4 cut(s) 426, 523, 766, 1020
HhaI GCGC 1 cut(s) 814
Hin1II CATG 5 cut(s) 183, 693, 1001, 1080, 1210
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HinfI GANTC 4 cut(s) 20, 365, 445, 716
HpaII CCGG 4 cut(s) 426, 523, 766, 1020
HphI GGTGA 5 cut(s) 512, 690, 762, 1004, 1164
Hpy166II GTNNAC 2 cut(s) 152, 295
Hpy188I TCNGA 9 cut(s) 25, 256, 271, 541, 580, 866, 981, 1014, 1123
Hpy188III TCNNGA 4 cut(s) 674, 785, 998, 1178
Hpy8I GTNNAC 2 cut(s) 152, 295
HpyAV CCTTC 3 cut(s) 20, 137, 1190
HpyCH4III ACNGT 4 cut(s) 65, 113, 877, 1057
HpyCH4IV ACGT 2 cut(s) 311, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 903
HpyF3I CTNAG 2 cut(s) 956, 1120
HpySE526I ACGT 2 cut(s) 311, 822
Hsp92II CATG 5 cut(s) 183, 693, 1001, 1080, 1210
HspAI GCGC 1 cut(s) 812
Ksp22I TGATCA 1 cut(s) 994
Kzo9I GATC 1 cut(s) 994
LmnI GCTCC 2 cut(s) 590, 676
Lsp1109I GCAGC 2 cut(s) 296, 838
LweI GCATC 7 cut(s) 202, 238, 260, 412, 472, 602, 884
MaeI CTAG 2 cut(s) 464, 1082
MaeII ACGT 2 cut(s) 311, 822
MaeIII GTNAC 2 cut(s) 768, 1166
MalI GATC 1 cut(s) 996
MboI GATC 1 cut(s) 994
MboII GAAGA 3 cut(s) 53, 901, 1040
MfeI CAATTG 1 cut(s) 629
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 7 cut(s) 499, 574, 629, 645, 788, 1008, 1043
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 9 cut(s) 62, 140, 347, 447, 613, 748, 773, 783, 1185
Mph1103I ATGCAT 1 cut(s) 253
MseI TTAA 6 cut(s) 305, 432, 648, 804, 1062, 1154
MslI CAYNNNNRTG 1 cut(s) 178
MspA1I CMGCKG 1 cut(s) 570
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 4 cut(s) 426, 523, 766, 1020
MspR9I CCNGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 629
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 248, 903
NcoI CCATGG 1 cut(s) 1076
NdeI CATATG 2 cut(s) 919, 1144
NdeII GATC 1 cut(s) 994
NlaIII CATG 5 cut(s) 183, 693, 1001, 1080, 1210
NlaIV GGNNCC 1 cut(s) 678
NmuCI GTSAC 2 cut(s) 768, 1166
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 1 cut(s) 1210
PagI TCATGA 1 cut(s) 997
PciI ACATGT 1 cut(s) 1206
PfeI GAWTC 3 cut(s) 365, 445, 716
PflMI CCANNNNNTGG 1 cut(s) 673
PkrI GCNGC 2 cut(s) 286, 853
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 823
PscI ACATGT 1 cut(s) 1206
Psp6I CCWGG 1 cut(s) 73
PspEI GGTNACC 1 cut(s) 768
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 678
PspPI GGNCC 3 cut(s) 71, 113, 967
PstNI CAGNNNCTG 1 cut(s) 707
PvuII CAGCTG 1 cut(s) 570
RsaI GTAC 7 cut(s) 62, 153, 509, 688, 705, 961, 1118
RsaNI GTAC 7 cut(s) 61, 152, 508, 687, 704, 960, 1117
RseI CAYNNNNRTG 1 cut(s) 178
SaqAI TTAA 6 cut(s) 305, 432, 648, 804, 1062, 1154
SatI GCNGC 2 cut(s) 285, 852
Sau3AI GATC 1 cut(s) 994
Sau96I GGNCC 3 cut(s) 71, 113, 967
ScaI AGTACT 1 cut(s) 1118
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 7 cut(s) 202, 238, 260, 412, 472, 602, 884
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 178
SmlI CTYRAG 4 cut(s) 304, 338, 449, 1176
SmoI CTYRAG 4 cut(s) 304, 338, 449, 1176
SnaBI TACGTA 1 cut(s) 823
Sse9I AATT 7 cut(s) 499, 574, 629, 645, 788, 1008, 1043
SsiI CCGC 2 cut(s) 147, 1135
SspMI CTAG 2 cut(s) 464, 1082
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 3 cut(s) 463, 963, 1076
TaaI ACNGT 4 cut(s) 65, 113, 877, 1057
TaiI ACGT 2 cut(s) 314, 825
TaqI TCGA 2 cut(s) 18, 847
TasI AATT 7 cut(s) 499, 574, 629, 645, 788, 1008, 1043
TatI WGTACW 3 cut(s) 507, 686, 1116
TfiI GAWTC 3 cut(s) 365, 445, 716
Tru1I TTAA 6 cut(s) 305, 432, 648, 804, 1062, 1154
Tru9I TTAA 6 cut(s) 305, 432, 648, 804, 1062, 1154
TscAI CASTG 5 cut(s) 70, 232, 861, 937, 1030
TseFI GTSAC 2 cut(s) 768, 1166
TseI GCWGC 2 cut(s) 284, 851
Tsp45I GTSAC 2 cut(s) 768, 1166
TspDTI ATGAA 5 cut(s) 4, 156, 558, 618, 1210
TspRI CASTG 5 cut(s) 70, 232, 861, 937, 1030
Van91I CCANNNNNTGG 1 cut(s) 673
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 2 cut(s) 499, 788
XceI RCATGY 1 cut(s) 1210
XcmI CCANNNNNNNNNTGG 1 cut(s) 687
XmaJI CCTAGG 1 cut(s) 463
XspI CTAG 2 cut(s) 464, 1082
ZrmI AGTACT 1 cut(s) 1118
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.