Rroxscaffold_7G00212800

Serine carboxypeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002263
Physical Location & Seq
Reverse (-)
63304505 .. 63320881
16377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212800.1

Sequence Viewer

Length: 5169 bp
ATGGAGAAAGTTTACTGTGGCACACTGGTCAAGTCTATTCCCGGGTTCGACGGTGAACTTCCTTTCAAGCTCTACACTGGGTATACGACTGTTAATGGCTCAGAGATGTTTTACTACTTCATTGAGTCAGAAGGAACCCCCAAAGAAGACCCTTTGTTGCTGTGGTACAGTGGAGGCCCTGGTTGTTCTGCTTTCAATGGTCTCATTTACGAAAACGGTCCCTTGGCTTTCAACGACACAGATTATGAAGGCGGTGTACCAAAAACATATTACTATCCATACTCATGGTCAAGGACTGCAAGCCTATTGTTTGTGGATGCACCTGTTGGCACTGGTTTCTCTTATGCAACTGATGCATCAGAATACGCTACCTCCGATACAAAAACAGCAGCACAGGTTTACCAGTTCTTGAGAACGTGGTTGAATGAGCACCCGCAATACCTCAAGGTTCAGTTGTTTGTTGGGGCTGATTCGTATTCAGGCGTATCGGGAACCATTGTTGTTGAACATATAATGGCTGGTAATGATGCCGGATTTAAGCCACGCCTGAATCTCAAGGGCTATATTCTAGGGTGTCCAAAAACAGATTCAACTATCAATGAAAATTCCAAGTACTCCTATTCTCACCGGATGGCACTTATATCAGATGAACTCTATTATCAAATTAAAGACATAAACAAAAACCATATTCTCGAGCCAAAATGCACATGGGCTTCACCAGTACCCGATGGAGATTCAGCTCGTAGATCTATGCAGGAATACGAATACCCCGAAGACTTCCTCCGGTCACCTCTAAAAAATCCTGAATTCTGTCACAACTTTAACTATGCGCTATCCTACGTATGGGCAAACGATGATGATGTCCGAGCGGCACTTAATATCAGAAATGGAACAGTATTTGATTGGAAGAGATGCAATAAGAGCTTGGATTACACATATGATGTGTCCAGTGTCCTTGAATATCATAAAAATCTCAGTACCAAGGGACTACAAGTTCTGATATTCAATGGTGATCATGACCTGACAATTCCGAACACCGGCACGGAAGAGTGGATAAAAAAATTGGGTTTGACTGTTGTTAATGACTGGAGACCATGGCTAGTTGATGGTCAGATTGCAGGATACACCATCAAGTATTCAGAAAATGGATACCGCTTGACATATGCAACCTTAAAGGGGGCAGGTCACTCACCTCAAGAATACAAGCGTAGAGAAGCAAATGCAGCTCACTGTGGCACATTGATCAAGTCCCTTCCGGGATTCGATGGTGAACTTCCTTTCAACCTCTACACTGGGTATACGACTGTTAAAGGATCAGAGATGTTTTACTACTTCGTTGAGTCGGAAGGAGACCCCAAAGTGGACCCTTTGTTGCTTTGGTACAGTGGTGGTCCTGGTTGTTCTGCTTTCAATGGCCTCATTTACGAAAATGGTCCTTTGGATTTCAATCTTACAGATTATGAAGGAGGTATACCGAAAACTTATCACTACCCATATTCATGGACAAGGACTGCGAGCATATTGTTTGTGGATGCACCTGTTGGCACTGGTTTCTCCTATGCTACTGATGCATCAGAATATGCTACCTCCGATACAAAAACAGCAGAGCAGGTTTACGGCTTTTTGACAGCGTGGTTGAATGAGCACCCGCAGTATTCCAAGCTTCAATTATTTGTTGGAGCTGATTCATATGCAGGAGTATCGGGAACCATTGTTGTTAAACATATAATAGATGGTAATGATGCTGGAGCATCACCACGCCTCAATCTCAAGGGCTATCTTTTAGGGTGTCCACGTACAGATGCAACCATCAATGAGAATTCGAAGTATATTTATGCTCACCGGATGGGACTTATATCAGATGACCTTTATCATTCTGCTTTAGAAAGCTGTAATGCAAGTTTCTATGGAGTAACCACAGATCAACCAGAATGTTATGAGGACCTTCAATTGATTGCAAGGCAAATTAAAGACATAAACAAAAACAATATTCTGGAGCCAAAGTGCACATGGGCTTCTCCAATAAACGATGAAGAACCGGCTCGTAGATCTATGCAGGAAAATGAATACCCTGAAGACTACCTCCGGTTACCTCTACAAAATCCTGAGTTCTGTCATACCTTCAGCTACTCTCTATCTTATGTTTGGGCGAACAGTGATGAAGTTCGAGCGGCGCTTAATATCAGAAATGGAACAGTATTTGATTGGAAGAGATGCAATAAGAGCTTGGATTACACATATGATGTGGCAAGTGTCCTCGAATATCATCAAAATCTCAGTACCAAGGGATTACAAGTTCTGATATTCAATGGTGATCATGACCTTATAATTCCGAACACCGGCACAGAAGCGTGGATAAAAAAGTTGGGTTTGACTATTGTTAATGATTGGAGACCATGGCTAGTTGATGGTCAAATTGCTGGGTACACCATCAAGTATTCAGAAAATGGATACCGCTTGTCATATGCAACCCTAAAGGGAGCAGCAAAGACAGTTCTTTGTGGCACACTGGTCAAGTCTATTCCGGGTTTCGATGGTGATCTTCCTTTTAAGCTCTACACTGGGTATGTCAGTGTGGGTAAAGCAGAGATGTTTTACTACTTCATTGAGTCAGAAGGAAACCCCAAAGAAGATCCTTTGTTGTTGTGGTATAGTGGAGGCCCTGGTTGTTCTGTTTTAAGTGGTATCATGTACGAAAATGGTCCTATGGTTTTCAATTATACAGCTTATGAAGGAGGGTTACCATCTACATATTACTACCCATACTCATGGACAAGGACTGCAAGTGTATTGTTTGTGGATGCACCTGTTGGCACTGGTTTCTCCTATGCAACTAGTGCAGATGAATTCCCTTCTTCAGATTTAAAAACAGCAGCACAGGTTTATGGATTTTTGAGGGAGTGGTTGGAAGTGCACCCACAATTTCTCAAAGTTCAGCTATTTATTGGGGCTGACTCATATTCAGGCCTGACGGGCACCATCATTGTCAAATACATAATAGATGACAATGATGCTGGAGTACTGCCACGACTCAACCTCAAGGGGTATGTTCTTGGGTGTCCAACTACTGATCAAACTCTCAATGAGAATGCAAGGTACGTTTATTCTCATCGGATGGGGCTTATATCAGATGAACTCTACGAGGCAGCTAAAGAAAGCTGCAATTCAAGTTTCTATGGTGCTACCACGTCAGAACCAGAATGCTATGAGGACGTTCAATTGATGGCAAAGCAAATTAAAGACATAAACAAACTCTGCATTCTAGAGCCAAAGTGCACGTGGGCTTCCCCAGACCAAGATGGAGAAACAGCTCATAGATATTTACATGAAAATCCCGAAGAATTCATCCGGTCACTTCCAAAAGATCCTGAATACTACTGTCGAAACTTCAATTATATGCTGGCTTATGTATGGGCAAACGATGATGGTGTAAGAAAGGCACTTAATGTCAGAGAGGGAACAGTACTAGATTGGAAGAGATGTAATAAAAGTTTGTTATACACAGAAGATGTGGAAAGTGTCATTGAGTATCATAAATACCTCAGTACCAAGGGATTACAAGTTCTGATATTCAATGGTGATCATGACCTTACAGTCCCAAACACCGGTACGGAACAGTGGATAAGAAGTTTGGATTTGCCTGTTGTCAATGATTGGAGACCGTGGCTAGTTGATGGTCAAATTGCTGGATACACCGTCAAGTATTCAAGTAGTGAGGGATACCGTTTGACTTATGCAACTGTAAAGGGTGCTGGGCACTCAGCTCCAGAATACAAGCGTAGGGAATGTTATATCATGTTTGATAGCTCTGAGGAGCTGTTCTACTCCGGCATTGCCACTTCTCAGACAATCGTTGAGACTCTGCCTGGGTGGAATGGGACACTTCCCTTCAAACTTGAAACAGGTTACATTGCGGTGGATGATTTGGATGAGGTGCAACTATTCTACTATTTTGTCGAGTCGGAGAGAAACCCAATTGACGATCCTCTTATGCTTTGGCTTACTGGAGGCCCTGGATGTTCCGGGTTTTCTGCAATGGCTTATGAAATTGGTCCATTTAGTTTTGATCTGCATTACAATGGAAGCATACCCACTATAATTATGAATCCATATTCATGGACCAAGGTGGCCAACATAATCTTTATAGATGCACCAGTTGGTGCTGGGTTTTCATACTCTACAACCTCTGAAGGGTACTATACTTCCGATACAAAATCAGCTGCACAGACCTATACTTTCTTAAGAAAGTGGTTATATGCACACCCAAGTTTTCAGGGAAATCAACTGTACATTGGTGGTGACTCATATTCAGGACTTATTGTTCCCATTCTTCTTAAGAATATACTAGAAGGTTTAGAGTCTGGGTCAAGGCCAAGAATGCAACTCCAAGGATACTTGCTTGGAAATCCAGTTTCAGATTCTTTTATTGATGTAAATGAGAGAATCCCATATGTTCACCGAGTGTCACTCATTTCAGATGAAATCTATGAGGCTGCTAAAACTAATTGCAGTGGGGACTATGTCAATGTGGAACTTAACAATACACTTTGTGTTACTGCTCTTCAAAAAATTAAAGATTGTCTTCTACAAATAAATCTTGCACAAATTTTGGAACCCCAATGTGCTTTTGCATCTGGTAGAACAACTGAGTTGGAGTGGGATCTTAGAGCTCGAGAAGCAAGTACTATGGATTACCTCCTTTCAGAGTCTAAGCTACCTGAATTGCCTTGCCGGGGATATACATATGTGCTTGCCTATAGGTGGATGAACGATGAAACAGTTCGGGCAGCTCTAGGTGTTCGAAATGGAACAGTAGAGAATTGGGTACGATGCCCCAAGGCCTTGGCATACTACACAGAAGAGATCACAAGTTCTCTTGCGTATCAGAAAAATCTATCAGAAACAGGTCTTCGAGCTTTGATATACAGTGGTGATCATGACATATCAGTTCCATGGATTGCTACACAATATTGGATCAAAGCTCTTGATATAGCTGTTTTTGATGAATGGCGACCGTGGTATGTCAACGGTCAAATTGCAGGATACCAAGTGAAGTTCATGAATGATCACTTCAGGTTGACTTACGGTGCTGGTCACACTGCTCCAGAGTACAAACACAAGGAAACACTAGCCATGTTTGACAGATTTTTAGCACGTTACCCGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

1722

Amino Acids

194.45

Weight (kDa)

4.94

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 14 - 406 1.7e-99 Serine carboxypeptidase
Peptidase_S10 PF00450 419 - 831 7.6e-104 Serine carboxypeptidase
Peptidase_S10 PF00450 842 - 1269 1.6e-109 Serine carboxypeptidase
Peptidase_S10 PF00450 1289 - 1718 2.5e-116 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2327
AasI GACNNNNNNGTC 1 cut(s) 3593
Acc36I ACCTGC 2 cut(s) 1172, 1600
AccB1I GGYRCC 1 cut(s) 2975
AccBSI CCGCTC 2 cut(s) 869, 2171
AccI GTMKAC 3 cut(s) 83, 1298, 1471
AciI CCGC 8 cut(s) 252, 434, 869, 1153, 1649, 2171, 2455, 3914
AclWI GGATC 6 cut(s) 1321, 2627, 3359, 3977, 4668, 4982
AcoI YGGCCR 1 cut(s) 4128
AcsI RAATTY 6 cut(s) 604, 806, 1819, 2846, 3341, 4605
AcuI CTGAAG 5 cut(s) 2094, 2107, 2841, 4209, 5056
AcvI CACGTG 1 cut(s) 3279
AdeI CACNNNGTG 2 cut(s) 4462, 4550
AfiI CCNNNNNNNGG 9 cut(s) 843, 1037, 1176, 1360, 2339, 3605, 4191, 4733, 4761
AflII CTTAAG 2 cut(s) 4240, 4334
AgeI ACCGGT 1 cut(s) 3605
AhlI ACTAGT 1 cut(s) 2834
AjiI CACGTC 1 cut(s) 3189
AjnI CCWGG 5 cut(s) 178, 1393, 2662, 3865, 4012
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AloI GAACNNNNNNTCC 8 cut(s) 978, 1010, 2280, 2312, 3546, 3578, 4305, 4337
Alw21I GWGCWC 6 cut(s) 432, 1647, 2009, 2916, 3278, 4672
Alw26I GTCTC 6 cut(s) 206, 1084, 1344, 2386, 3652, 3851
Alw44I GTGCAC 3 cut(s) 2005, 2912, 3274
AlwI GGATC 6 cut(s) 1321, 2627, 3359, 3977, 4668, 4982
Ama87I CYCGRG 3 cut(s) 41, 692, 4671
AoxI GGCC 8 cut(s) 175, 1414, 2659, 2965, 4009, 4128, 4370, 4839
ApaLI GTGCAC 3 cut(s) 2005, 2912, 3274
ApeKI GCWGC 9 cut(s) 389, 1223, 2483, 2873, 3146, 3159, 4220, 4493, 4787
ApoI RAATTY 6 cut(s) 604, 806, 1819, 2846, 3341, 4605
AsiGI ACCGGT 1 cut(s) 3605
Asp700I GAANNNNTTC 1 cut(s) 4779
AspLEI GCGC 2 cut(s) 832, 2176
AsuC2I CCSGG 6 cut(s) 42, 43, 1257, 2526, 4024, 4733
AsuII TTCGAA 2 cut(s) 1823, 4801
AvaI CYCGRG 3 cut(s) 41, 692, 4671
AvaII GGWCC 8 cut(s) 218, 1363, 1391, 1433, 1942, 2702, 4052, 4119
BaeGI GKGCMC 5 cut(s) 2009, 2916, 2978, 3278, 3759
BaeI ACNNNNGTAYC 4 cut(s) 1372, 1405, 3529, 3562
BalI TGGCCA 1 cut(s) 4130
BanI GGYRCC 1 cut(s) 2975
BanII GRGCYC 1 cut(s) 4672
BarI GAAGNNNNNNTAC 6 cut(s) 240, 272, 1455, 1487, 2724, 2756
BbrPI CACGTG 1 cut(s) 3279
BbsI GAAGAC 5 cut(s) 153, 780, 2082, 4574, 4901
Bbv12I GWGCWC 6 cut(s) 432, 1647, 2009, 2916, 3278, 4672
BbvI GCAGC 9 cut(s) 401, 1235, 2495, 2885, 3146, 3158, 4207, 4480, 4799
BceAI ACGGC 1 cut(s) 1633
BcgI CGANNNNNNTGC 2 cut(s) 1683, 1717
BciT130I CCWGG 5 cut(s) 180, 1395, 2664, 3867, 4014
BciVI GTATCC 7 cut(s) 1115, 1142, 2444, 3683, 3713, 4385, 5036
BclI TGATCA 7 cut(s) 1012, 1242, 2314, 3070, 3580, 4933, 5065
BcnI CCSGG 6 cut(s) 42, 43, 1257, 2526, 4024, 4733
BcoDI GTCTC 6 cut(s) 206, 1084, 1344, 2386, 3652, 3851
BcuI ACTAGT 1 cut(s) 2834
BfmI CTRYAG 1 cut(s) 4756
BfoI RGCGCY 1 cut(s) 2177
BfrI CTTAAG 2 cut(s) 4240, 4334
BfuAI ACCTGC 2 cut(s) 1172, 1600
BfuI GTATCC 7 cut(s) 1115, 1142, 2444, 3683, 3713, 4385, 5036
BglI GCCNNNNNGGC 1 cut(s) 2973
BglII AGATCT 2 cut(s) 746, 2048
BmcAI AGTACT 4 cut(s) 614, 3021, 3465, 4684
Bme18I GGWCC 8 cut(s) 218, 1363, 1391, 1433, 1942, 2702, 4052, 4119
BmeT110I CYCGRG 3 cut(s) 41, 692, 4671
BmgBI CACGTC 1 cut(s) 3189
BmiI GGNNCC 8 cut(s) 136, 220, 493, 1365, 1708, 1998, 2977, 4614
BmrI ACTGGG 3 cut(s) 87, 1302, 2571
BmuI ACTGGG 3 cut(s) 87, 1302, 2571
BpiI GAAGAC 5 cut(s) 153, 780, 2082, 4574, 4901
BplI GAGNNNNNCTC 2 cut(s) 4452, 4484
BpmI CTGGAG 7 cut(s) 1108, 1767, 2015, 3036, 3750, 4026, 5088
Bpu14I TTCGAA 2 cut(s) 1823, 4801
BpuEI CTTGAG 6 cut(s) 428, 430, 539, 1179, 1754, 3023
BpuMI CCSGG 6 cut(s) 42, 43, 1257, 2526, 4024, 4733
BsaAI YACGTR 3 cut(s) 841, 1796, 3279
BsaBI GATNNNNATC 2 cut(s) 1446, 2538
BsaI GGTCTC 5 cut(s) 206, 1084, 1344, 2386, 3652
BsaWI WCCGGW 6 cut(s) 627, 783, 1842, 2085, 3348, 3605
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 1988, 2018
Bsc4I CCNNNNNNNGG 9 cut(s) 843, 1037, 1176, 1360, 2339, 3605, 4191, 4733, 4761
Bse118I RCCGGY 4 cut(s) 1037, 2038, 2339, 3605
Bse3DI GCAATG 3 cut(s) 3831, 3909, 4041
Bse8I GATNNNNATC 2 cut(s) 1446, 2538
BseBI CCWGG 5 cut(s) 180, 1395, 2664, 3867, 4014
BseJI GATNNNNATC 2 cut(s) 1446, 2538
BseLI CCNNNNNNNGG 9 cut(s) 843, 1037, 1176, 1360, 2339, 3605, 4191, 4733, 4761
BseMI GCAATG 3 cut(s) 3831, 3909, 4041
BseMII CTCAG 9 cut(s) 114, 988, 2097, 2290, 3556, 3774, 3801, 3857, 4638
BseRI GAGGAG 1 cut(s) 3827
BseSI GKGCMC 5 cut(s) 2009, 2916, 2978, 3278, 3759
BseXI GCAGC 9 cut(s) 401, 1235, 2495, 2885, 3146, 3158, 4207, 4480, 4799
BseYI CCCAGC 3 cut(s) 2420, 3752, 4163
BsgI GTGCAG 2 cut(s) 2859, 4206
Bsh1285I CGRYCG 1 cut(s) 5015
BshFI GGCC 8 cut(s) 177, 1416, 2661, 2967, 4011, 4130, 4372, 4841
BshNI GGYRCC 1 cut(s) 2975
BshTI ACCGGT 1 cut(s) 3605
BsiEI CGRYCG 1 cut(s) 5015
BsiHKAI GWGCWC 6 cut(s) 432, 1647, 2009, 2916, 3278, 4672
BsiHKCI CYCGRG 3 cut(s) 41, 692, 4671
BslFI GGGAC 7 cut(s) 204, 999, 1234, 1863, 3581, 3892, 4529
BslI CCNNNNNNNGG 9 cut(s) 843, 1037, 1176, 1360, 2339, 3605, 4191, 4733, 4761
BsmAI GTCTC 6 cut(s) 206, 1084, 1344, 2386, 3652, 3851
BsmFI GGGAC 7 cut(s) 204, 999, 1234, 1863, 3581, 3892, 4529
BsmI GAATGC 4 cut(s) 3094, 3206, 3258, 4383
BsnI GGCC 8 cut(s) 177, 1416, 2661, 2967, 4011, 4130, 4372, 4841
Bso31I GGTCTC 5 cut(s) 206, 1084, 1344, 2386, 3652
BsoBI CYCGRG 3 cut(s) 41, 692, 4671
Bsp119I TTCGAA 2 cut(s) 1823, 4801
Bsp1286I GDGCHC 8 cut(s) 432, 1647, 2009, 2916, 2978, 3278, 3759, 4672
Bsp1407I TGTACA 1 cut(s) 4287
Bsp19I CCATGG 3 cut(s) 1094, 2396, 4952
BspACI CCGC 8 cut(s) 252, 434, 869, 1153, 1649, 2171, 2455, 3914
BspANI GGCC 8 cut(s) 177, 1416, 2661, 2967, 4011, 4130, 4372, 4841
BspCNI CTCAG 9 cut(s) 113, 987, 2098, 2289, 3555, 3773, 3802, 3856, 4639
BspHI TCATGA 5 cut(s) 1015, 2317, 3583, 4936, 5058
BspLI GGNNCC 8 cut(s) 136, 220, 493, 1365, 1708, 1998, 2977, 4614
BspMI ACCTGC 2 cut(s) 1172, 1600
BspPI GGATC 6 cut(s) 1321, 2627, 3359, 3977, 4668, 4982
BspQI GCTCTTC 1 cut(s) 4566
BspT104I TTCGAA 2 cut(s) 1823, 4801
BspT107I GGYRCC 1 cut(s) 2975
BspTI CTTAAG 2 cut(s) 4240, 4334
BspTNI GGTCTC 5 cut(s) 206, 1084, 1344, 2386, 3652
BsrBI CCGCTC 2 cut(s) 869, 2171
BsrDI GCAATG 3 cut(s) 3831, 3909, 4041
BsrFI RCCGGY 4 cut(s) 1037, 2038, 2339, 3605
BsrGI TGTACA 1 cut(s) 4287
BssAI RCCGGY 4 cut(s) 1037, 2038, 2339, 3605
BssNAI GTATAC 3 cut(s) 84, 1299, 1472
Bst1107I GTATAC 3 cut(s) 84, 1299, 1472
Bst2UI CCWGG 5 cut(s) 180, 1395, 2664, 3867, 4014
Bst6I CTCTTC 6 cut(s) 902, 1041, 2204, 3470, 4566, 4854
BstAFI CTTAAG 2 cut(s) 4240, 4334
BstAPI GCANNNNNTGC 2 cut(s) 353, 2837
BstAUI TGTACA 1 cut(s) 4287
BstBAI YACGTR 3 cut(s) 841, 1796, 3279
BstBI TTCGAA 2 cut(s) 1823, 4801
BstC8I GCNNGC 4 cut(s) 301, 1516, 3402, 4752
BstDSI CCRYGG 5 cut(s) 1094, 2396, 3662, 4952, 5015
BstEII GGTNACC 3 cut(s) 786, 2088, 2739
BstH2I RGCGCY 1 cut(s) 2177
BstHHI GCGC 2 cut(s) 832, 2176
BstMAI GTCTC 6 cut(s) 206, 1084, 1344, 2386, 3652, 3851
BstMCI CGRYCG 1 cut(s) 5015
BstMWI GCNNNNNNNGC 9 cut(s) 353, 921, 1223, 1568, 2223, 2837, 2973, 4378, 4676
BstNI CCWGG 5 cut(s) 180, 1395, 2664, 3867, 4014
BstPI GGTNACC 3 cut(s) 786, 2088, 2739
BstSFI CTRYAG 1 cut(s) 4756
BstSLI GKGCMC 5 cut(s) 2009, 2916, 2978, 3278, 3759
BstSNI TACGTA 1 cut(s) 841
BstV1I GCAGC 9 cut(s) 401, 1235, 2495, 2885, 3146, 3158, 4207, 4480, 4799
BstV2I GAAGAC 5 cut(s) 153, 780, 2082, 4574, 4901
BstX2I RGATCY 5 cut(s) 746, 2048, 2632, 3364, 4660
BstXI CCANNNNNNTGG 5 cut(s) 285, 1500, 2769, 4116, 4843
BstYI RGATCY 5 cut(s) 746, 2048, 2632, 3364, 4660
BstZ17I GTATAC 3 cut(s) 84, 1299, 1472
BsuI GTATCC 7 cut(s) 1115, 1142, 2444, 3683, 3713, 4385, 5036
BsuRI GGCC 8 cut(s) 177, 1416, 2661, 2967, 4011, 4130, 4372, 4841
BtgI CCRYGG 5 cut(s) 1094, 2396, 3662, 4952, 5015
BtrI CACGTC 1 cut(s) 3189
BtsI GCAGTG 2 cut(s) 4516, 5097
BveI ACCTGC 2 cut(s) 1172, 1600
Cac8I GCNNGC 4 cut(s) 301, 1516, 3402, 4752
CciI TCATGA 5 cut(s) 1015, 2317, 3583, 4936, 5058
CfoI GCGC 2 cut(s) 832, 2176
Cfr10I RCCGGY 4 cut(s) 1037, 2038, 2339, 3605
Cfr9I CCCGGG 1 cut(s) 41
CspAI ACCGGT 1 cut(s) 3605
DraI TTTAAA 1 cut(s) 2865
DraIII CACNNNGTG 2 cut(s) 4462, 4550
DrdI GACNNNNNNGTC 1 cut(s) 3593
DseDI GACNNNNNNGTC 1 cut(s) 3593
EaeI YGGCCR 1 cut(s) 4128
Eam1104I CTCTTC 6 cut(s) 902, 1041, 2204, 3470, 4566, 4854
EarI CTCTTC 6 cut(s) 902, 1041, 2204, 3470, 4566, 4854
Ecl136II GAGCTC 1 cut(s) 4670
Eco105I TACGTA 1 cut(s) 841
Eco147I AGGCCT 2 cut(s) 2967, 4841
Eco24I GRGCYC 1 cut(s) 4672
Eco31I GGTCTC 5 cut(s) 206, 1084, 1344, 2386, 3652
Eco47I GGWCC 8 cut(s) 218, 1363, 1391, 1433, 1942, 2702, 4052, 4119
Eco53kI GAGCTC 1 cut(s) 4670
Eco57I CTGAAG 5 cut(s) 2094, 2107, 2841, 4209, 5056
Eco72I CACGTG 1 cut(s) 3279
Eco88I CYCGRG 3 cut(s) 41, 692, 4671
Eco91I GGTNACC 3 cut(s) 786, 2088, 2739
EcoICRI GAGCTC 1 cut(s) 4670
EcoO109I RGGNCCY 4 cut(s) 176, 1942, 2660, 4010
EcoO65I GGTNACC 3 cut(s) 786, 2088, 2739
EcoRI GAATTC 4 cut(s) 806, 1819, 2846, 3341
EcoRII CCWGG 5 cut(s) 178, 1393, 2662, 3865, 4012
EcoT22I ATGCAT 2 cut(s) 358, 1573
EcoT38I GRGCYC 1 cut(s) 4672
FalI AAGNNNNNCTT 2 cut(s) 4566, 4598
FaqI GGGAC 7 cut(s) 204, 999, 1234, 1863, 3581, 3892, 4529
FauI CCCGC 2 cut(s) 441, 1656
FauNDI CATATG 7 cut(s) 937, 1162, 1690, 2239, 2464, 4450, 4744
FbaI TGATCA 7 cut(s) 1012, 1242, 2314, 3070, 3580, 4933, 5065
FblI GTMKAC 3 cut(s) 83, 1298, 1471
FriOI GRGCYC 1 cut(s) 4672
GlaI GCGC 2 cut(s) 831, 2175
GsaI CCCAGC 3 cut(s) 2424, 3756, 4167
GsuI CTGGAG 7 cut(s) 1108, 1767, 2015, 3036, 3750, 4026, 5088
HaeII RGCGCY 1 cut(s) 2177
HaeIII GGCC 8 cut(s) 177, 1416, 2661, 2967, 4011, 4130, 4372, 4841
HhaI GCGC 2 cut(s) 832, 2176
Hin6I GCGC 2 cut(s) 830, 2174
HinP1I GCGC 2 cut(s) 830, 2174
HincII GTYRAC 2 cut(s) 5026, 5079
HindII GTYRAC 2 cut(s) 5026, 5079
HindIII AAGCTT 1 cut(s) 1661
Hpy99I CGWCG 1 cut(s) 53
HpyCH4IV ACGT 8 cut(s) 416, 840, 1795, 3099, 3188, 3213, 3278, 5155
HpyF10VI GCNNNNNNNGC 9 cut(s) 353, 921, 1223, 1568, 2223, 2837, 2973, 4378, 4676
HpySE526I ACGT 8 cut(s) 416, 840, 1795, 3099, 3188, 3213, 3278, 5155
HspAI GCGC 2 cut(s) 830, 2174
Ksp22I TGATCA 7 cut(s) 1012, 1242, 2314, 3070, 3580, 4933, 5065
LguI GCTCTTC 1 cut(s) 4566
LmnI GCTCC 7 cut(s) 1679, 1748, 1996, 2480, 3769, 3814, 5107
Lsp1109I GCAGC 9 cut(s) 401, 1235, 2495, 2885, 3146, 3158, 4207, 4480, 4799
MaeII ACGT 8 cut(s) 416, 840, 1795, 3099, 3188, 3213, 3278, 5155
MbiI CCGCTC 2 cut(s) 869, 2171
MfeI CAATTG 3 cut(s) 1949, 3218, 3975
MflI RGATCY 5 cut(s) 746, 2048, 2632, 3364, 4660
MhlI GDGCHC 8 cut(s) 432, 1647, 2009, 2916, 2978, 3278, 3759, 4672
MlsI TGGCCA 1 cut(s) 4130
MluNI TGGCCA 1 cut(s) 4130
MmeI TCCRAC 6 cut(s) 1323, 1657, 2886, 3086, 3942, 4632
Mox20I TGGCCA 1 cut(s) 4130
Mph1103I ATGCAT 2 cut(s) 358, 1573
MroXI GAANNNNTTC 1 cut(s) 4779
MscI TGGCCA 1 cut(s) 4130
MslI CAYNNNNRTG 6 cut(s) 283, 1498, 2767, 3914, 4077, 4114
Msp20I TGGCCA 1 cut(s) 4130
MspA1I CMGCKG 1 cut(s) 4220
MspCI CTTAAG 2 cut(s) 4240, 4334
MunI CAATTG 3 cut(s) 1949, 3218, 3975
Mva1269I GAATGC 4 cut(s) 3094, 3206, 3258, 4383
MvaI CCWGG 5 cut(s) 180, 1395, 2664, 3867, 4014
MwoI GCNNNNNNNGC 9 cut(s) 353, 921, 1223, 1568, 2223, 2837, 2973, 4378, 4676
NciI CCSGG 6 cut(s) 42, 43, 1257, 2526, 4024, 4733
NcoI CCATGG 3 cut(s) 1094, 2396, 4952
NdeI CATATG 7 cut(s) 937, 1162, 1690, 2239, 2464, 4450, 4744
NlaIV GGNNCC 8 cut(s) 136, 220, 493, 1365, 1708, 1998, 2977, 4614
NmuCI GTSAC 7 cut(s) 786, 812, 1184, 3351, 4298, 4464, 5093
NsiI ATGCAT 2 cut(s) 358, 1573
NspV TTCGAA 2 cut(s) 1823, 4801
PaeR7I CTCGAG 2 cut(s) 692, 4671
PagI TCATGA 5 cut(s) 1015, 2317, 3583, 4936, 5058
PceI AGGCCT 2 cut(s) 2967, 4841
PciSI GCTCTTC 1 cut(s) 4566
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 768
PctI GAATGC 4 cut(s) 3094, 3206, 3258, 4383
PdmI GAANNNNTTC 1 cut(s) 4779
PfeI GAWTC 9 cut(s) 470, 550, 587, 734, 1260, 1685, 4105, 4418, 4443
PflFI GACNNNGTC 1 cut(s) 4520
PfoI TCCNGGA 1 cut(s) 1255
PinAI ACCGGT 1 cut(s) 3605
PmaCI CACGTG 1 cut(s) 3279
PmlI CACGTG 1 cut(s) 3279
Ppu21I YACGTR 3 cut(s) 841, 1796, 3279
PpuMI RGGWCCY 1 cut(s) 1942
PsiI TTATAA 1 cut(s) 2327
Psp124BI GAGCTC 1 cut(s) 4672
Psp5II RGGWCCY 1 cut(s) 1942
Psp6I CCWGG 5 cut(s) 178, 1393, 2662, 3865, 4012
PspCI CACGTG 1 cut(s) 3279
PspEI GGTNACC 3 cut(s) 786, 2088, 2739
PspFI CCCAGC 3 cut(s) 2420, 3752, 4163
PspGI CCWGG 5 cut(s) 178, 1393, 2662, 3865, 4012
PspN4I GGNNCC 8 cut(s) 136, 220, 493, 1365, 1708, 1998, 2977, 4614
PspPPI RGGWCCY 1 cut(s) 1942
PsuI RGATCY 5 cut(s) 746, 2048, 2632, 3364, 4660
PsyI GACNNNGTC 1 cut(s) 4520
PvuII CAGCTG 1 cut(s) 4220
RseI CAYNNNNRTG 6 cut(s) 283, 1498, 2767, 3914, 4077, 4114
SacI GAGCTC 1 cut(s) 4672
SapI GCTCTTC 1 cut(s) 4566
ScaI AGTACT 4 cut(s) 614, 3021, 3465, 4684
SduI GDGCHC 8 cut(s) 432, 1647, 2009, 2916, 2978, 3278, 3759, 4672
SfcI CTRYAG 1 cut(s) 4756
Sfr274I CTCGAG 2 cut(s) 692, 4671
SfuI TTCGAA 2 cut(s) 1823, 4801
SinI GGWCC 8 cut(s) 218, 1363, 1391, 1433, 1942, 2702, 4052, 4119
SlaI CTCGAG 2 cut(s) 692, 4671
SmaI CCCGGG 1 cut(s) 43
SmiMI CAYNNNNRTG 6 cut(s) 283, 1498, 2767, 3914, 4077, 4114
SnaBI TACGTA 1 cut(s) 841
SpeI ACTAGT 1 cut(s) 2834
SseBI AGGCCT 2 cut(s) 2967, 4841
SsiI CCGC 8 cut(s) 252, 434, 869, 1153, 1649, 2171, 2455, 3914
SspI AATATT 2 cut(s) 1990, 4970
SstI GAGCTC 1 cut(s) 4672
StuI AGGCCT 2 cut(s) 2967, 4841
TaiI ACGT 8 cut(s) 419, 843, 1798, 3102, 3191, 3216, 3281, 5158
TatI WGTACW 6 cut(s) 612, 3019, 3463, 4287, 4682, 5109
TauI GCSGC 2 cut(s) 872, 2174
TfiI GAWTC 9 cut(s) 470, 550, 587, 734, 1260, 1685, 4105, 4418, 4443
TseFI GTSAC 7 cut(s) 786, 812, 1184, 3351, 4298, 4464, 5093
TseI GCWGC 9 cut(s) 389, 1223, 2483, 2873, 3146, 3159, 4220, 4493, 4787
Tsp45I GTSAC 7 cut(s) 786, 812, 1184, 3351, 4298, 4464, 5093
TspGWI ACGGA 2 cut(s) 1058, 3626
TspMI CCCGGG 1 cut(s) 41
Tth111I GACNNNGTC 1 cut(s) 4520
Vha464I CTTAAG 2 cut(s) 4240, 4334
VneI GTGCAC 3 cut(s) 2005, 2912, 3274
VpaK11BI GGWCC 8 cut(s) 218, 1363, 1391, 1433, 1942, 2702, 4052, 4119
XapI RAATTY 6 cut(s) 604, 806, 1819, 2846, 3341, 4605
XbaI TCTAGA 1 cut(s) 3262
XcmI CCANNNNNNNNNTGG 3 cut(s) 705, 2007, 3276
XhoI CTCGAG 2 cut(s) 692, 4671
XmaI CCCGGG 1 cut(s) 41
XmiI GTMKAC 3 cut(s) 83, 1298, 1471
XmnI GAANNNNTTC 1 cut(s) 4779
ZrmI AGTACT 4 cut(s) 614, 3021, 3465, 4684
Zsp2I ATGCAT 2 cut(s) 358, 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.