Rh6AG052000

Serine carboxypeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002263
Physical Location & Seq
Forward (+)
7487038 .. 7490401
3364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG052000.1

Sequence Viewer

Length: 1419 bp
ATGGTATTTGGTTCTTGCAACAAAACCATGAACTGGGTTTGTTTGCATTTGCTTCTGCTACTAATTTTCTCGGCCAATGCAGTTTACTGTGGCACACTGGTCAAGTCTATTCCCGGGTTCGATGGTGAACTTCCTTTCAAGCTCTACACTGGGTATACGACTGTTAATGGCTCAGAGATGTTTTACTACTTCATTGAGTCAGAAGGAACCCCCAAACAAGACCCTTTGTTGCTGTGGTACAGTGGAGGCCCTGGTTGTTCTGCTTTCAATGGTCTCATTTACGAAAACGGTCCCTTGGCTTTCAACGACACAGATTATGAAGGCGGTATACCAAAAACATTTTACTATCCATACTCATGGTCAAGGACTGCAAGCCTATTGTTTGTGGATGCACCTGTTGGCACTGGTTTCTCTTATGCAACTGATGCATCAGAATACGCTACCTCCGATACAAAAACAGCAGCACAGGTTTACCAGTTCTTGAGAACGTGGTTGAATGAGCACCCGCAATACCTCAAGGTTCAGTTGTTTGTTGGGGCTGATTCGTATTCAGGCATATCGGGAACCATTGTTGTTGAACATATAATAGCTGGTAATGATGCCGGATTTAAGCCACGCCTGAATCTCAAGGGCTATATTCTAGGGTGTCCAAGAACAGATGAAACTATCAATGAGAATTCTAAGTACTCCTATTCTCACCGGATGGCACTTATATCAGATGAACTCTATTATTCGGCTAAAAACAGCTGTAATTCAGATTTCTATGGAGCAACTGTTGATGAACCAGAATGTTACGAGGATGTTCAATTGATTGCAAGGCAAATTAAAGACATAAACAAAAACCATATTCTGGAGCCAAAATGCACATGGGCTTCACCAGTACCCGATGGAGAATCAGCTCGTAGATCTATGCAGGAATACGAATACCCCGAAGACTTCCTCCGGTCACCTCTAAGAAATCCTGAATTCTGTCACAACTTTAACTATGCGCTATCCTACGTATGGGCAAACGATGATGATGTACGAGCGGCACTTCATATCAGAAATGGAACAGTATTTGATTGGAAGAGATGCAACAAGAGCTTGGATTACACATATGATGTGTCCAGTGTCCTAGAATATCATCAAAATCTCAGTACCAAGGGACTACAAGTTCTGATATTCAATGGTGATCATGACCTGACAATTCCGAACACCGGCACGGAAGAGTGGATAAAAAAATTGGGTTTGACTGTTGTTAATGACTGGAGACCATGGCTAGTTGATGGTCAGATTGCAGGATACACCATCAAGTATTCAGAAAATGGATACCGCTTGACATATGCAACCTTAAAGGGGGCAGGTCACTCACCTCAAGAATACAAGCGTAGAGAAGGTTATGACATGTTCGACAGGTTCATCCATTACTATCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

472

Amino Acids

53.77

Weight (kDa)

5.16

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 468 2.3e-110 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1331
AccB7I CCANNNNNTGG 2 cut(s) 33, 850
AccBSI CCGCTC 1 cut(s) 1028
AccI GTMKAC 2 cut(s) 155, 328
AciI CCGC 4 cut(s) 324, 506, 1028, 1312
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 2 cut(s) 676, 965
AfaI GTAC 5 cut(s) 239, 686, 882, 1023, 1138
AfiI CCNNNNNNNGG 5 cut(s) 33, 850, 1002, 1196, 1335
AflIII ACRYGT 1 cut(s) 1383
AgsI TTSAA 7 cut(s) 139, 268, 304, 496, 578, 806, 1165
AjnI CCWGG 1 cut(s) 250
AloI GAACNNNNNNTCC 2 cut(s) 1137, 1169
AluBI AGCT 5 cut(s) 142, 590, 747, 899, 1083
AluI AGCT 5 cut(s) 142, 590, 747, 899, 1083
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 2 cut(s) 278, 1243
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 2 cut(s) 72, 247
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 676, 965
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 2 cut(s) 248, 290
AsuC2I CCSGG 2 cut(s) 114, 115
AsuHPI GGTGA 6 cut(s) 137, 689, 867, 939, 1181, 1341
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 290
BarI GAAGNNNNNNTAC 2 cut(s) 312, 344
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 473
BccI CCATC 5 cut(s) 116, 697, 881, 1259, 1295
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 2 cut(s) 1274, 1301
BclI TGATCA 1 cut(s) 1171
BcnI CCSGG 2 cut(s) 114, 115
BcoDI GTCTC 2 cut(s) 278, 1243
BfaI CTAG 4 cut(s) 641, 1115, 1259, 1417
BfuAI ACCTGC 1 cut(s) 1331
BfuI GTATCC 2 cut(s) 1274, 1301
BglII AGATCT 1 cut(s) 905
BisI GCNGC 2 cut(s) 462, 1029
BlsI GCNGC 2 cut(s) 463, 1030
BmcAI AGTACT 1 cut(s) 686
Bme1390I CCNGG 3 cut(s) 114, 115, 252
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 248, 290
BmiI GGNNCC 4 cut(s) 208, 292, 565, 855
BmrFI CCNGG 3 cut(s) 114, 115, 252
BmrI ACTGGG 2 cut(s) 43, 159
BmsI GCATC 5 cut(s) 379, 415, 437, 589, 1061
BmuI ACTGGG 2 cut(s) 43, 159
BpiI GAAGAC 1 cut(s) 939
BpmI CTGGAG 2 cut(s) 872, 1267
BpuEI CTTGAG 4 cut(s) 500, 502, 611, 1338
BpuMI CCSGG 2 cut(s) 114, 115
BsaAI YACGTR 1 cut(s) 1000
BsaI GGTCTC 2 cut(s) 278, 1243
BsaJI CCNNGG 5 cut(s) 113, 250, 294, 1140, 1253
BsaWI WCCGGW 2 cut(s) 699, 942
BsaXI ACNNNNNCTCC 4 cut(s) 428, 458, 759, 789
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 850, 1002, 1196, 1335
Bse118I RCCGGY 1 cut(s) 1196
Bse1I ACTGG 8 cut(s) 38, 102, 154, 409, 475, 878, 1107, 1250
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 5 cut(s) 113, 250, 294, 1140, 1253
BseGI GGATG 4 cut(s) 394, 708, 805, 1398
BseLI CCNNNNNNNGG 5 cut(s) 33, 850, 1002, 1196, 1335
BseMII CTCAG 2 cut(s) 186, 1147
BseNI ACTGG 8 cut(s) 38, 102, 154, 409, 475, 878, 1107, 1250
BseXI GCAGC 1 cut(s) 473
BshFI GGCC 2 cut(s) 74, 249
BsiHKAI GWGCWC 1 cut(s) 504
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 5 cut(s) 114, 603, 700, 943, 1197
BslFI GGGAC 2 cut(s) 276, 1158
BslI CCNNNNNNNGG 5 cut(s) 33, 850, 1002, 1196, 1335
BsmAI GTCTC 2 cut(s) 278, 1243
BsmFI GGGAC 2 cut(s) 276, 1158
BsnI GGCC 2 cut(s) 74, 249
Bso31I GGTCTC 2 cut(s) 278, 1243
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 2 cut(s) 905, 1171
Bsp19I CCATGG 1 cut(s) 1253
BspACI CCGC 4 cut(s) 324, 506, 1028, 1312
BspANI GGCC 2 cut(s) 74, 249
BspCNI CTCAG 2 cut(s) 185, 1146
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 4 cut(s) 208, 292, 565, 855
BspMI ACCTGC 1 cut(s) 1331
BspTNI GGTCTC 2 cut(s) 278, 1243
BsrBI CCGCTC 1 cut(s) 1028
BsrFI RCCGGY 1 cut(s) 1196
BsrI ACTGG 8 cut(s) 38, 102, 154, 409, 475, 878, 1107, 1250
BssAI RCCGGY 1 cut(s) 1196
BssECI CCNNGG 5 cut(s) 113, 250, 294, 1140, 1253
BssMI GATC 2 cut(s) 905, 1171
BssNAI GTATAC 2 cut(s) 156, 329
BssT1I CCWWGG 3 cut(s) 294, 1140, 1253
Bst1107I GTATAC 2 cut(s) 156, 329
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 7 cut(s) 89, 163, 242, 290, 775, 1054, 1234
Bst6I CTCTTC 2 cut(s) 1061, 1200
BstAPI GCANNNNNTGC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 1000
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 4 cut(s) 172, 681, 953, 1133
BstDSI CCRYGG 1 cut(s) 1253
BstEII GGTNACC 1 cut(s) 945
BstF5I GGATG 4 cut(s) 394, 708, 805, 1398
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 2 cut(s) 908, 1174
BstMAI GTCTC 2 cut(s) 278, 1243
BstMBI GATC 2 cut(s) 905, 1171
BstMWI GCNNNNNNNGC 2 cut(s) 425, 1080
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 1387
BstPI GGTNACC 1 cut(s) 945
BstSCI CCNGG 3 cut(s) 112, 113, 250
BstSNI TACGTA 1 cut(s) 1000
BstV1I GCAGC 1 cut(s) 473
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 1 cut(s) 905
BstXI CCANNNNNNTGG 1 cut(s) 357
BstYI RGATCY 1 cut(s) 905
BstZ17I GTATAC 2 cut(s) 156, 329
BsuI GTATCC 2 cut(s) 1274, 1301
BsuRI GGCC 2 cut(s) 74, 249
BtgI CCRYGG 1 cut(s) 1253
BtsCI GGATG 4 cut(s) 394, 708, 805, 1398
BtsIMutI CAGTG 5 cut(s) 95, 147, 247, 402, 1114
BveI ACCTGC 1 cut(s) 1331
Cac8I GCNNGC 1 cut(s) 373
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 1196
Cfr13I GGNCC 2 cut(s) 248, 290
Cfr9I CCCGGG 1 cut(s) 113
Csp6I GTAC 5 cut(s) 238, 685, 881, 1022, 1137
CviAII CATG 6 cut(s) 28, 357, 867, 1175, 1254, 1384
CviQI GTAC 5 cut(s) 238, 685, 881, 1022, 1137
DdeI CTNAG 4 cut(s) 172, 681, 953, 1133
DpnI GATC 2 cut(s) 907, 1173
DpnII GATC 2 cut(s) 905, 1171
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 2 cut(s) 1061, 1200
EarI CTCTTC 2 cut(s) 1061, 1200
Eco105I TACGTA 1 cut(s) 1000
Eco130I CCWWGG 3 cut(s) 294, 1140, 1253
Eco31I GGTCTC 2 cut(s) 278, 1243
Eco47I GGWCC 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 113
Eco91I GGTNACC 1 cut(s) 945
EcoO109I RGGNCCY 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 945
EcoRI GAATTC 2 cut(s) 676, 965
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 3 cut(s) 294, 1140, 1253
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 3 cut(s) 294, 1140, 1253
FaeI CATG 6 cut(s) 31, 360, 870, 1178, 1257, 1387
FaqI GGGAC 2 cut(s) 276, 1158
FatI CATG 6 cut(s) 27, 356, 866, 1174, 1253, 1383
FauI CCCGC 1 cut(s) 513
FauNDI CATATG 2 cut(s) 1096, 1321
FbaI TGATCA 1 cut(s) 1171
FblI GTMKAC 2 cut(s) 155, 328
Fnu4HI GCNGC 2 cut(s) 462, 1029
FokI GGATG 4 cut(s) 401, 715, 812, 1385
Fsp4HI GCNGC 2 cut(s) 462, 1029
FspBI CTAG 4 cut(s) 641, 1115, 1259, 1417
GlaI GCGC 1 cut(s) 990
GluI GCNGC 2 cut(s) 462, 1029
GsuI CTGGAG 2 cut(s) 872, 1267
HaeIII GGCC 2 cut(s) 74, 249
HapII CCGG 5 cut(s) 114, 603, 700, 943, 1197
HhaI GCGC 1 cut(s) 991
Hin1II CATG 6 cut(s) 31, 360, 870, 1178, 1257, 1387
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HinfI GANTC 4 cut(s) 197, 542, 622, 893
HpaII CCGG 5 cut(s) 114, 603, 700, 943, 1197
HphI GGTGA 6 cut(s) 137, 689, 867, 939, 1181, 1341
Hpy166II GTNNAC 5 cut(s) 85, 128, 156, 329, 472
Hpy188III TCNNGA 6 cut(s) 481, 561, 851, 962, 1175, 1355
Hpy8I GTNNAC 5 cut(s) 85, 128, 156, 329, 472
HpyAV CCTTC 3 cut(s) 197, 314, 1367
HpyCH4III ACNGT 7 cut(s) 89, 163, 242, 290, 775, 1054, 1234
HpyCH4IV ACGT 2 cut(s) 488, 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 1080
HpyF3I CTNAG 4 cut(s) 172, 681, 953, 1133
HpySE526I ACGT 2 cut(s) 488, 999
Hsp92II CATG 6 cut(s) 31, 360, 870, 1178, 1257, 1387
HspAI GCGC 1 cut(s) 989
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 2 cut(s) 905, 1171
LmnI GCTCC 2 cut(s) 767, 853
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 5 cut(s) 379, 415, 437, 589, 1061
MaeI CTAG 4 cut(s) 641, 1115, 1259, 1417
MaeII ACGT 2 cut(s) 488, 999
MaeIII GTNAC 4 cut(s) 791, 945, 971, 1343
MalI GATC 2 cut(s) 907, 1173
MbiI CCGCTC 1 cut(s) 1028
MboI GATC 2 cut(s) 905, 1171
MboII GAAGA 3 cut(s) 944, 1078, 1217
MfeI CAATTG 1 cut(s) 806
MflI RGATCY 1 cut(s) 905
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 8 cut(s) 63, 676, 751, 806, 822, 965, 1185, 1220
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 239, 454, 524, 790, 950, 960, 1362
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 6 cut(s) 165, 609, 825, 981, 1239, 1331
MslI CAYNNNNRTG 1 cut(s) 355
MspA1I CMGCKG 1 cut(s) 747
MspI CCGG 5 cut(s) 114, 603, 700, 943, 1197
MspR9I CCNGG 3 cut(s) 114, 115, 252
MunI CAATTG 1 cut(s) 806
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 425, 1080
NciI CCSGG 2 cut(s) 114, 115
NcoI CCATGG 1 cut(s) 1253
NdeI CATATG 2 cut(s) 1096, 1321
NdeII GATC 2 cut(s) 905, 1171
NlaIII CATG 6 cut(s) 31, 360, 870, 1178, 1257, 1387
NlaIV GGNNCC 4 cut(s) 208, 292, 565, 855
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 3 cut(s) 945, 971, 1343
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 1387
PagI TCATGA 1 cut(s) 1174
PciI ACATGT 1 cut(s) 1383
PcsI WCGNNNNNNNCGW 2 cut(s) 444, 927
PfeI GAWTC 3 cut(s) 542, 622, 893
PflMI CCANNNNNTGG 2 cut(s) 33, 850
PkrI GCNGC 2 cut(s) 463, 1030
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Ppu21I YACGTR 1 cut(s) 1000
PscI ACATGT 1 cut(s) 1383
Psp6I CCWGG 1 cut(s) 250
PspEI GGTNACC 1 cut(s) 945
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 4 cut(s) 208, 292, 565, 855
PspPI GGNCC 2 cut(s) 248, 290
PsuI RGATCY 1 cut(s) 905
PvuII CAGCTG 1 cut(s) 747
RsaI GTAC 5 cut(s) 239, 686, 882, 1023, 1138
RsaNI GTAC 5 cut(s) 238, 685, 881, 1022, 1137
RseI CAYNNNNRTG 1 cut(s) 355
SaqAI TTAA 6 cut(s) 165, 609, 825, 981, 1239, 1331
SatI GCNGC 2 cut(s) 462, 1029
Sau3AI GATC 2 cut(s) 905, 1171
Sau96I GGNCC 2 cut(s) 248, 290
ScaI AGTACT 1 cut(s) 686
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 3 cut(s) 114, 115, 252
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 5 cut(s) 379, 415, 437, 589, 1061
SinI GGWCC 1 cut(s) 290
SmaI CCCGGG 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 355
SmlI CTYRAG 4 cut(s) 481, 515, 626, 1353
SmoI CTYRAG 4 cut(s) 481, 515, 626, 1353
SnaBI TACGTA 1 cut(s) 1000
Sse9I AATT 8 cut(s) 63, 676, 751, 806, 822, 965, 1185, 1220
SsiI CCGC 4 cut(s) 324, 506, 1028, 1312
SspMI CTAG 4 cut(s) 641, 1115, 1259, 1417
StyD4I CCNGG 3 cut(s) 112, 113, 250
StyI CCWWGG 3 cut(s) 294, 1140, 1253
TaaI ACNGT 7 cut(s) 89, 163, 242, 290, 775, 1054, 1234
TaiI ACGT 2 cut(s) 491, 1002
TaqI TCGA 2 cut(s) 120, 1389
TasI AATT 8 cut(s) 63, 676, 751, 806, 822, 965, 1185, 1220
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 1031
TfiI GAWTC 3 cut(s) 542, 622, 893
Tru1I TTAA 6 cut(s) 165, 609, 825, 981, 1239, 1331
Tru9I TTAA 6 cut(s) 165, 609, 825, 981, 1239, 1331
TscAI CASTG 5 cut(s) 102, 154, 247, 409, 1114
TseFI GTSAC 3 cut(s) 945, 971, 1343
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 3 cut(s) 945, 971, 1343
TspDTI ATGAA 8 cut(s) 44, 181, 333, 675, 735, 795, 1025, 1387
TspGWI ACGGA 1 cut(s) 1217
TspMI CCCGGG 1 cut(s) 113
TspRI CASTG 5 cut(s) 102, 154, 247, 409, 1114
Van91I CCANNNNNTGG 2 cut(s) 33, 850
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 2 cut(s) 676, 965
XceI RCATGY 1 cut(s) 1387
XcmI CCANNNNNNNNNTGG 1 cut(s) 864
XmaI CCCGGG 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 155, 328
XspI CTAG 4 cut(s) 641, 1115, 1259, 1417
ZrmI AGTACT 1 cut(s) 686
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.