FvH4_2g05020

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003578
Physical Location & Seq
Forward (+)
4075063 .. 4076390
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05020.t1

Sequence Viewer

Length: 684 bp
ATGGCTCAAAACAATATTTTTCTCTTCTCCCTTCTCATAACACTTGCAGGCATTCACGCCGCGGATGCAGTCGACTATGCAGTCACCAACACCGCTGGATCTAGTGCCGGTGCCGTCCGATTTAACAACGACATCGGAGCAGACTATAGCTTGCAAACCCTAGCCTCTGCGGCAGACTTCATATGGAAAGTCTTCCAACAAAGCACAGCTGCAGATAGAAAGAGCGTAGACAAAGTCAGCTTGTTCATCGACAATATGGACGGCGTGGCGTATGCTTCCGGCGGTGAGATTCATGTTAGCGCCAGCTACATAGCAGGCTACTCAGGCGACGTGAAAAAGGAGATTACGGGGGTGCTCTACCATGAATCGACGCACGTATGGCAGTGGAATGGGAATGGTCAAGCCATCCCAGGTGGATTGATTGAGGGCATTGCAGACTTTGTGAGGTTGAGGGCTGGGTATGCACCGAGTCACTGGGTTCAGCCAGGTGAAGGAGATCGCTGGGACCAGGGTTATGATGTTACCGCTAGGTTTTTGGATTACTGTACTAGTTTGAAAGATGGGTTTGTTGCAGAATTGAACAACAAGTTAAAGACTGGTTATAGTGTTAACTACTTTGCTGATATCTTGGGGAAGAACGTTGATCAGCTCTGGGCTGATTACAAGGCTAAATATGGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

228

Amino Acids

24.7

Weight (kDa)

5.11

Isoelectric Point (pI)

21.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 23 - 221 7.5e-78 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 2 cut(s) 72, 228
AccII CGCG 1 cut(s) 62
AciI CCGC 6 cut(s) 60, 62, 93, 170, 282, 525
AclI AACGTT 1 cut(s) 639
AclWI GGATC 1 cut(s) 106
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 1 cut(s) 491
AgsI TTSAA 2 cut(s) 556, 580
AhlI ACTAGT 1 cut(s) 548
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 3 cut(s) 409, 484, 507
AluBI AGCT 5 cut(s) 150, 209, 240, 306, 649
AluI AGCT 5 cut(s) 150, 209, 240, 306, 649
Alw21I GWGCWC 1 cut(s) 357
AlwI GGATC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 3 cut(s) 76, 296, 500
AvaII GGWCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 196
BccI CCATC 2 cut(s) 413, 554
BceAI ACGGC 2 cut(s) 98, 277
BciT130I CCWGG 3 cut(s) 411, 486, 509
BclI TGATCA 1 cut(s) 643
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 4 cut(s) 102, 161, 528, 549
BfmI CTRYAG 2 cut(s) 145, 210
BfoI RGCGCY 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 3 cut(s) 60, 171, 210
BlsI GCNGC 3 cut(s) 61, 172, 211
Bme1390I CCNGG 3 cut(s) 411, 486, 509
Bme18I GGWCC 1 cut(s) 505
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 112, 506
BmrFI CCNGG 3 cut(s) 411, 486, 509
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 55
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 3 cut(s) 60, 409, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 479, 601
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 3 cut(s) 411, 486, 509
BseDI CCNNGG 3 cut(s) 60, 409, 508
BseGI GGATG 2 cut(s) 70, 405
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 479, 601
BseXI GCAGC 1 cut(s) 196
BseYI CCCAGC 2 cut(s) 455, 501
Bsh1236I CGCG 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 2 cut(s) 108, 279
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 3 cut(s) 98, 496, 643
BspACI CCGC 6 cut(s) 60, 62, 93, 170, 282, 525
BspCNI CTCAG 1 cut(s) 335
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 112, 506
BspMAI CTGCAG 1 cut(s) 214
BspPI GGATC 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 2 cut(s) 479, 601
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 3 cut(s) 60, 409, 508
BssMI GATC 3 cut(s) 98, 496, 643
Bst2UI CCWGG 3 cut(s) 411, 486, 509
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 4 cut(s) 49, 152, 304, 316
BstDEI CTNAG 1 cut(s) 322
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 2 cut(s) 70, 405
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 3 cut(s) 101, 499, 646
BstMBI GATC 3 cut(s) 98, 496, 643
BstMWI GCNNNNNNNGC 5 cut(s) 65, 170, 324, 379, 461
BstNI CCWGG 3 cut(s) 411, 486, 509
BstSCI CCNGG 3 cut(s) 409, 484, 507
BstSFI CTRYAG 2 cut(s) 145, 210
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 70, 405
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 2 cut(s) 389, 472
Cac8I GCNNGC 4 cut(s) 49, 152, 304, 316
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 505
Cfr42I CCGCGG 1 cut(s) 63
CseI GACGC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 546
CviAII CATG 2 cut(s) 293, 362
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 1 cut(s) 322
DpnI GATC 3 cut(s) 100, 498, 645
DpnII GATC 3 cut(s) 98, 496, 643
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 505
EcoRII CCWGG 3 cut(s) 409, 484, 507
EcoRV GATATC 1 cut(s) 625
FaeI CATG 2 cut(s) 296, 365
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 292, 361
FauNDI CATATG 1 cut(s) 182
FbaI TGATCA 1 cut(s) 643
FblI GTMKAC 2 cut(s) 72, 228
Fnu4HI GCNGC 3 cut(s) 60, 171, 210
FokI GGATG 2 cut(s) 77, 392
Fsp4HI GCNGC 3 cut(s) 60, 171, 210
FspBI CTAG 4 cut(s) 102, 161, 528, 549
GlaI GCGC 1 cut(s) 301
GluI GCNGC 3 cut(s) 60, 171, 210
GsaI CCCAGC 2 cut(s) 459, 505
HaeII RGCGCY 1 cut(s) 303
HapII CCGG 2 cut(s) 108, 279
HgaI GACGC 1 cut(s) 379
HhaI GCGC 1 cut(s) 302
Hin1II CATG 2 cut(s) 296, 365
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 2 cut(s) 73, 610
HindII GTYRAC 2 cut(s) 73, 610
HinfI GANTC 3 cut(s) 289, 365, 469
HpaI GTTAAC 1 cut(s) 610
HpaII CCGG 2 cut(s) 108, 279
HphI GGTGA 3 cut(s) 76, 296, 500
Hpy166II GTNNAC 3 cut(s) 73, 229, 610
Hpy188I TCNGA 2 cut(s) 119, 137
Hpy8I GTNNAC 3 cut(s) 73, 229, 610
Hpy99I CGWCG 2 cut(s) 332, 373
HpyAV CCTTC 2 cut(s) 41, 485
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 3 cut(s) 330, 375, 639
HpyCH4V TGCA 8 cut(s) 47, 68, 80, 154, 212, 434, 464, 572
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 170, 324, 379, 461
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 3 cut(s) 330, 375, 639
Hsp92II CATG 2 cut(s) 296, 365
HspAI GCGC 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 643
KspAI GTTAAC 1 cut(s) 610
KspI CCGCGG 1 cut(s) 63
Kzo9I GATC 3 cut(s) 98, 496, 643
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 55
MaeI CTAG 4 cut(s) 102, 161, 528, 549
MaeII ACGT 3 cut(s) 330, 375, 639
MaeIII GTNAC 3 cut(s) 82, 470, 520
MalI GATC 3 cut(s) 100, 498, 645
MboI GATC 3 cut(s) 98, 496, 643
MboII GAAGA 3 cut(s) 16, 184, 646
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 1 cut(s) 575
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 4 cut(s) 175, 418, 438, 444
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 3 cut(s) 123, 590, 609
MspA1I CMGCKG 3 cut(s) 62, 95, 209
MspI CCGG 2 cut(s) 108, 279
MspR9I CCNGG 3 cut(s) 411, 486, 509
Mva1269I GAATGC 1 cut(s) 51
MvaI CCWGG 3 cut(s) 411, 486, 509
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 65, 170, 324, 379, 461
NdeI CATATG 1 cut(s) 182
NdeII GATC 3 cut(s) 98, 496, 643
NlaIII CATG 2 cut(s) 296, 365
NlaIV GGNNCC 2 cut(s) 112, 506
NmuCI GTSAC 2 cut(s) 82, 470
PctI GAATGC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 289, 365
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 61, 172, 211
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Ppu21I YACGTR 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 639
Psp6I CCWGG 3 cut(s) 409, 484, 507
PspFI CCCAGC 2 cut(s) 455, 501
PspGI CCWGG 3 cut(s) 409, 484, 507
PspN4I GGNNCC 2 cut(s) 112, 506
PspPI GGNCC 1 cut(s) 505
PstI CTGCAG 1 cut(s) 214
PsuI RGATCY 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SacII CCGCGG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 3 cut(s) 123, 590, 609
SatI GCNGC 3 cut(s) 60, 171, 210
Sau3AI GATC 3 cut(s) 98, 496, 643
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 3 cut(s) 411, 486, 509
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 2 cut(s) 145, 210
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SinI GGWCC 1 cut(s) 505
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 1 cut(s) 575
SsiI CCGC 6 cut(s) 60, 62, 93, 170, 282, 525
SspI AATATT 1 cut(s) 16
SspMI CTAG 4 cut(s) 102, 161, 528, 549
StyD4I CCNGG 3 cut(s) 409, 484, 507
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 3 cut(s) 333, 378, 642
TaqI TCGA 3 cut(s) 72, 249, 368
TasI AATT 1 cut(s) 575
TatI WGTACW 1 cut(s) 545
TauI GCSGC 2 cut(s) 62, 173
TfiI GAWTC 2 cut(s) 289, 365
Tru1I TTAA 3 cut(s) 123, 590, 609
Tru9I TTAA 3 cut(s) 123, 590, 609
TscAI CASTG 2 cut(s) 389, 479
TseFI GTSAC 2 cut(s) 82, 470
TseI GCWGC 1 cut(s) 209
Tsp45I GTSAC 2 cut(s) 82, 470
TspDTI ATGAA 4 cut(s) 169, 235, 281, 378
TspRI CASTG 2 cut(s) 389, 479
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 505
XmiI GTMKAC 2 cut(s) 72, 228
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 4 cut(s) 102, 161, 528, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.