MD10G1147400.v1.1

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003578
Physical Location & Seq
Reverse (-)
23543640 .. 23544332
693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1147400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGTTCAATTTCACCATATTTGTTTCCTCTCCTTGCTCATAACACTAGCAGCAGGCTTCCTTCCTGGCACCCATGCAGTCGACTACAGCGTCACAAATACCGCCAGTGGAACCCCCGGCGGAATCCGCTTCAACAATGAAATCGGAGCAGACTACGGCAGGCAAACCCTAATCTCCGCGACCGACTTCATATGGAGCTTGTTCCAGCAAAAAACAACCGATGACAGAAAGAGTGTCTCCAAGGTCACCCTAATCGTTGAAAACATCACCGGGGTCGCGTACACCATCAACAACGAGATCCACGTCAGCGCCAGCTACATCGCTGGTTACTCCGGCAATGTGAAGAGTGAGATCACCGGTGTGCTTTACCATGAGTCAACGCACGTCTGGCAATGGAATGGGAACGGTCGAGCCCCGGGGGGGCTGATTGAGGGTATAGCTGATTACGTGAGGTTGAAGGCCGGATACGCGCCAGGTCACTGGGTTAAGCCCGGGCAGGGGGATAGGTGGGACCAGGGGTACGATGTCACTGCTAGGTTTCTTGACTATTTGAATAGTTTGAAAAATGGGTTTGTTGCTGAAATGAATAAGAAATTGAGGAGTGGTTATAGTGCTGATTATTTTGTTGATTTGCTGGGGAAGAATGTTGAACAGATTTGGAGAGAGTACAAGGCCAAGTATGCAGGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

231

Amino Acids

25.5

Weight (kDa)

7.06

Isoelectric Point (pI)

19.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 26 - 223 2e-79 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 179, 278, 470
AciI CCGC 4 cut(s) 102, 120, 127, 177
AclWI GGATC 1 cut(s) 292
AfaI GTAC 3 cut(s) 281, 521, 668
AfiI CCNNNNNNNGG 3 cut(s) 420, 497, 498
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 7 cut(s) 8, 133, 260, 457, 553, 562, 650
AjiI CACGTC 2 cut(s) 304, 385
AjnI CCWGG 3 cut(s) 64, 472, 513
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 3 cut(s) 198, 315, 440
AluI AGCT 3 cut(s) 198, 315, 440
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 2 cut(s) 415, 491
AoxI GGCC 2 cut(s) 459, 672
ApeKI GCWGC 1 cut(s) 50
AsiGI ACCGGT 1 cut(s) 356
AspLEI GCGC 2 cut(s) 311, 472
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 6 cut(s) 117, 271, 416, 417, 492, 493
AsuHPI GGTGA 4 cut(s) 5, 238, 259, 346
AvaI CYCGRG 2 cut(s) 415, 491
AvaII GGWCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 415
BarI GAAGNNNNNNTAC 2 cut(s) 449, 481
BbvI GCAGC 1 cut(s) 62
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 172
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 3 cut(s) 66, 474, 515
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 6 cut(s) 117, 271, 416, 417, 492, 493
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 2 cut(s) 47, 534
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 312
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 9 cut(s) 66, 117, 271, 416, 417, 474, 492, 493, 515
Bme18I GGWCC 1 cut(s) 511
BmeT110I CYCGRG 2 cut(s) 415, 491
BmgBI CACGTC 2 cut(s) 304, 385
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 3 cut(s) 70, 112, 512
BmrFI CCNGG 9 cut(s) 66, 117, 271, 416, 417, 474, 492, 493, 515
BmrI ACTGGG 1 cut(s) 490
BmuI ACTGGG 1 cut(s) 490
BpuMI CCSGG 6 cut(s) 117, 271, 416, 417, 492, 493
BsaAI YACGTR 1 cut(s) 448
BsaJI CCNNGG 8 cut(s) 115, 240, 270, 414, 415, 416, 491, 514
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 3 cut(s) 420, 497, 498
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 105, 485
Bse3DI GCAATG 2 cut(s) 343, 398
BseBI CCWGG 3 cut(s) 66, 474, 515
BseDI CCNNGG 8 cut(s) 115, 240, 270, 414, 415, 416, 491, 514
BseLI CCNNNNNNNGG 3 cut(s) 420, 497, 498
BseMI GCAATG 2 cut(s) 343, 398
BseNI ACTGG 2 cut(s) 105, 485
BseRI GAGGAG 1 cut(s) 613
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 634
Bsh1236I CGCG 3 cut(s) 179, 278, 470
Bsh1285I CGRYCG 2 cut(s) 183, 409
BshFI GGCC 2 cut(s) 461, 674
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 356
BsiEI CGRYCG 2 cut(s) 183, 409
BsiHKCI CYCGRG 2 cut(s) 415, 491
BsiSI CCGG 7 cut(s) 117, 270, 333, 357, 416, 462, 492
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 3 cut(s) 420, 497, 498
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 2 cut(s) 461, 674
BsoBI CYCGRG 2 cut(s) 415, 491
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 2 cut(s) 297, 351
BspACI CCGC 4 cut(s) 102, 120, 127, 177
BspANI GGCC 2 cut(s) 461, 674
BspFNI CGCG 3 cut(s) 179, 278, 470
BspLI GGNNCC 3 cut(s) 70, 112, 512
BspPI GGATC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 343, 398
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 2 cut(s) 105, 485
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 8 cut(s) 115, 240, 270, 414, 415, 416, 491, 514
BssMI GATC 2 cut(s) 297, 351
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 3 cut(s) 66, 474, 515
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 3 cut(s) 55, 161, 313
BstEII GGTNACC 1 cut(s) 244
BstFNI CGCG 3 cut(s) 179, 278, 470
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 311, 472
BstKTI GATC 2 cut(s) 300, 354
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 2 cut(s) 297, 351
BstMCI CGRYCG 2 cut(s) 183, 409
BstMWI GCNNNNNNNGC 4 cut(s) 126, 388, 467, 680
BstNI CCWGG 3 cut(s) 66, 474, 515
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 9 cut(s) 64, 115, 269, 414, 415, 472, 490, 491, 513
BstSFI CTRYAG 1 cut(s) 85
BstUI CGCG 3 cut(s) 179, 278, 470
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 297
BstXI CCANNNNNNTGG 1 cut(s) 480
BstYI RGATCY 1 cut(s) 297
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 461, 674
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 2 cut(s) 304, 385
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 3 cut(s) 112, 478, 528
Cac8I GCNNGC 3 cut(s) 55, 161, 313
CfoI GCGC 2 cut(s) 311, 472
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 511
Cfr9I CCCGGG 2 cut(s) 415, 491
CseI GACGC 1 cut(s) 79
Csp6I GTAC 3 cut(s) 280, 520, 667
CspAI ACCGGT 1 cut(s) 356
CviAII CATG 2 cut(s) 74, 371
CviJI RGCY 9 cut(s) 57, 198, 315, 413, 424, 440, 461, 490, 674
CviKI_1 RGCY 9 cut(s) 57, 198, 315, 413, 424, 440, 461, 490, 674
CviQI GTAC 3 cut(s) 280, 520, 667
DpnI GATC 2 cut(s) 299, 353
DpnII GATC 2 cut(s) 297, 351
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
EciI GGCGGA 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 511
Eco88I CYCGRG 2 cut(s) 415, 491
Eco91I GGTNACC 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 244
EcoRII CCWGG 3 cut(s) 64, 472, 513
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 77, 374
FaiI YATR 9 cut(s) 18, 41, 75, 191, 193, 372, 437, 609, 681
FaqI GGGAC 1 cut(s) 524
FatI CATG 2 cut(s) 73, 370
FauNDI CATATG 1 cut(s) 191
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 51
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 47, 534
GlaI GCGC 2 cut(s) 310, 471
GluI GCNGC 1 cut(s) 51
GsaI CCCAGC 1 cut(s) 638
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 2 cut(s) 461, 674
HapII CCGG 7 cut(s) 117, 270, 333, 357, 416, 462, 492
HgaI GACGC 1 cut(s) 79
HhaI GCGC 2 cut(s) 311, 472
Hin1II CATG 2 cut(s) 77, 374
Hin6I GCGC 2 cut(s) 309, 470
HinP1I GCGC 2 cut(s) 309, 470
HincII GTYRAC 2 cut(s) 82, 378
HindII GTYRAC 2 cut(s) 82, 378
HinfI GANTC 2 cut(s) 123, 374
HpaII CCGG 7 cut(s) 117, 270, 333, 357, 416, 462, 492
HphI GGTGA 4 cut(s) 5, 238, 259, 346
Hpy166II GTNNAC 3 cut(s) 82, 282, 378
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 1 cut(s) 542
Hpy8I GTNNAC 3 cut(s) 82, 282, 378
HpyAV CCTTC 2 cut(s) 71, 451
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 3 cut(s) 303, 384, 447
HpyCH4V TGCA 2 cut(s) 77, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 388, 467, 680
HpySE526I ACGT 3 cut(s) 303, 384, 447
Hsp92II CATG 2 cut(s) 77, 374
HspAI GCGC 2 cut(s) 309, 470
Kzo9I GATC 2 cut(s) 297, 351
LmnI GCTCC 2 cut(s) 146, 195
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 2 cut(s) 47, 534
MaeII ACGT 3 cut(s) 303, 384, 447
MaeIII GTNAC 5 cut(s) 91, 244, 326, 476, 526
MalI GATC 2 cut(s) 299, 353
MboI GATC 2 cut(s) 297, 351
MboII GAAGA 2 cut(s) 355, 652
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 2 cut(s) 8, 593
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 4 cut(s) 38, 424, 444, 591
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 7 cut(s) 117, 270, 333, 357, 416, 462, 492
MspR9I CCNGG 9 cut(s) 66, 117, 271, 416, 417, 474, 492, 493, 515
MvaI CCWGG 3 cut(s) 66, 474, 515
MvnI CGCG 3 cut(s) 179, 278, 470
MwoI GCNNNNNNNGC 4 cut(s) 126, 388, 467, 680
NciI CCSGG 6 cut(s) 117, 271, 416, 417, 492, 493
NdeI CATATG 1 cut(s) 191
NdeII GATC 2 cut(s) 297, 351
NlaIII CATG 2 cut(s) 77, 374
NlaIV GGNNCC 3 cut(s) 70, 112, 512
NmuCI GTSAC 4 cut(s) 91, 244, 476, 526
OliI CACNNNNGTG 1 cut(s) 359
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 300
PfeI GAWTC 1 cut(s) 123
PinAI ACCGGT 1 cut(s) 356
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 448
Psp6I CCWGG 3 cut(s) 64, 472, 513
PspEI GGTNACC 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 3 cut(s) 64, 472, 513
PspN4I GGNNCC 3 cut(s) 70, 112, 512
PspPI GGNCC 1 cut(s) 511
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 3 cut(s) 281, 521, 668
RsaNI GTAC 3 cut(s) 280, 520, 667
RseI CAYNNNNRTG 1 cut(s) 359
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 2 cut(s) 297, 351
Sau96I GGNCC 1 cut(s) 511
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 9 cut(s) 66, 117, 271, 416, 417, 474, 492, 493, 515
SduI GDGCHC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 356
SinI GGWCC 1 cut(s) 511
SmaI CCCGGG 2 cut(s) 417, 493
SmiMI CAYNNNNRTG 1 cut(s) 359
SrfI GCCCGGGC 1 cut(s) 493
Sse9I AATT 2 cut(s) 8, 593
SsiI CCGC 4 cut(s) 102, 120, 127, 177
SspMI CTAG 2 cut(s) 47, 534
StyD4I CCNGG 9 cut(s) 64, 115, 269, 414, 415, 472, 490, 491, 513
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 3 cut(s) 306, 387, 450
TaqI TCGA 2 cut(s) 81, 409
TaqII GACCGA 1 cut(s) 197
TasI AATT 2 cut(s) 8, 593
TatI WGTACW 1 cut(s) 666
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TscAI CASTG 3 cut(s) 112, 485, 535
TseFI GTSAC 4 cut(s) 91, 244, 476, 526
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 4 cut(s) 91, 244, 476, 526
TspDTI ATGAA 3 cut(s) 153, 178, 599
TspMI CCCGGG 2 cut(s) 415, 491
TspRI CASTG 3 cut(s) 112, 485, 535
VpaK11BI GGWCC 1 cut(s) 511
XmaI CCCGGG 2 cut(s) 415, 491
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 2 cut(s) 47, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.