MD05G1090800.v1.1

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003578
Physical Location & Seq
Reverse (-)
19250795 .. 19251490
696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1090800.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGTTCAATTTCACCACATCCGTTTCCTCTCCTTTCTCATAATAACACTAGCAGCAGGCCTCTTCCCGGGCACCCATGCAGTCAACTACAAAGTCACCAACACCGCGGGGGGAACCCCAGGCGGAATTCGGTTCAACAACAAAATCGGAGCAGACTATAGTAGGCAAACCCTAGTCTCCGCCACCAACTTCATATGGATCTTGTTCCAGCAAAACACAGCCGCGGACAGAAAGAGCGTCTCCAAGGTAACCTTAATTGTTGAAAACATCACCGGGGTGGCTTTTGCCGTCAACAATGAGATCCACGTCAGCGCCAGCTACATAGCTGGTTACTCTGGCGATGTGAAGAGGGAGATCACCGGTGTGCTTTACCATGAGTCAACGCATGTGTGGCAATGGAATGGGAACGGTCAAGCCCCAGGGGGGTTGATTGAGGGAATTGCTGATTATGTGAGGTTGAAGGCAGGATACGCGCCAAGTCATTGGGTTCAGCCGGGGCAAGGGGATAGGTGGGACCAGGGGTACGACGTCACTGCTTGGTTTCTAGACTATTTGAATAGTTTGAAAAATGGTTTTGTAGCTGAAATGAATAAGAAAATGAGGAGTGCTTATAATGCTAATTATTTTGTTGATATTCTGGGGAAGAATGTTGATCAGCTTTGGAGTGAGTACAAGGCCAAGTATGGAGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

232

Amino Acids

25.71

Weight (kDa)

9.13

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 27 - 224 4.3e-78 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AatII GACGTC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 71
AccII CGCG 3 cut(s) 107, 224, 473
AciI CCGC 6 cut(s) 105, 107, 123, 180, 222, 224
AclWI GGATC 2 cut(s) 206, 295
AcsI RAATTY 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 2 cut(s) 524, 671
AfiI CCNNNNNNNGG 3 cut(s) 67, 423, 500
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 6 cut(s) 8, 136, 263, 460, 556, 565
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 3 cut(s) 118, 418, 516
AleI CACNNNNGTG 2 cut(s) 275, 362
AluBI AGCT 4 cut(s) 318, 326, 581, 658
AluI AGCT 4 cut(s) 318, 326, 581, 658
Alw26I GTCTC 2 cut(s) 181, 244
AlwI GGATC 2 cut(s) 206, 295
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 2 cut(s) 58, 675
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 126
AsiGI ACCGGT 1 cut(s) 359
AspLEI GCGC 2 cut(s) 314, 475
AspS9I GGNCC 1 cut(s) 514
AsuC2I CCSGG 4 cut(s) 68, 69, 274, 495
AsuHPI GGTGA 4 cut(s) 5, 88, 262, 349
AvaI CYCGRG 1 cut(s) 67
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 74
BanI GGYRCC 1 cut(s) 71
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
BbvI GCAGC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 272
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 3 cut(s) 120, 420, 518
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 4 cut(s) 68, 69, 274, 495
BcoDI GTCTC 2 cut(s) 181, 244
BfaI CTAG 3 cut(s) 50, 173, 545
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 315
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 2 cut(s) 54, 222
BlsI GCNGC 2 cut(s) 55, 223
Bme1390I CCNGG 7 cut(s) 68, 69, 120, 274, 420, 495, 518
Bme18I GGWCC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 67
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 3 cut(s) 73, 115, 515
BmrFI CCNGG 7 cut(s) 68, 69, 120, 274, 420, 495, 518
BpuMI CCSGG 4 cut(s) 68, 69, 274, 495
BsaHI GRCGYC 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 423, 500
Bse118I RCCGGY 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 3 cut(s) 120, 420, 518
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 3 cut(s) 67, 423, 500
BseMI GCAATG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 616
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 1 cut(s) 65
Bsh1236I CGCG 3 cut(s) 107, 224, 473
BshFI GGCC 2 cut(s) 60, 677
BshNI GGYRCC 1 cut(s) 71
BshTI ACCGGT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 4 cut(s) 68, 273, 360, 494
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 3 cut(s) 67, 423, 500
BsmAI GTCTC 2 cut(s) 181, 244
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 2 cut(s) 60, 677
BsoBI CYCGRG 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 4 cut(s) 198, 300, 354, 652
BspACI CCGC 6 cut(s) 105, 107, 123, 180, 222, 224
BspANI GGCC 2 cut(s) 60, 677
BspFNI CGCG 3 cut(s) 107, 224, 473
BspLI GGNNCC 3 cut(s) 73, 115, 515
BspPI GGATC 2 cut(s) 206, 295
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 359
BssMI GATC 4 cut(s) 198, 300, 354, 652
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 3 cut(s) 120, 420, 518
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 2 cut(s) 68, 341
BstACI GRCGYC 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 58, 316
BstDSI CCRYGG 2 cut(s) 105, 222
BstEII GGTNACC 1 cut(s) 247
BstF5I GGATG 1 cut(s) 18
BstFNI CGCG 3 cut(s) 107, 224, 473
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 314, 475
BstKTI GATC 4 cut(s) 201, 303, 357, 655
BstMAI GTCTC 2 cut(s) 181, 244
BstMBI GATC 4 cut(s) 198, 300, 354, 652
BstMWI GCNNNNNNNGC 3 cut(s) 391, 470, 614
BstNI CCWGG 3 cut(s) 120, 420, 518
BstNSI RCATGY 1 cut(s) 389
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 7 cut(s) 66, 67, 118, 272, 418, 493, 516
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 74
BstUI CGCG 3 cut(s) 107, 224, 473
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 2 cut(s) 198, 300
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 2 cut(s) 198, 300
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 60, 677
BtgI CCRYGG 2 cut(s) 105, 222
BtgZI GCGATG 1 cut(s) 354
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 1 cut(s) 18
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 2 cut(s) 58, 316
CfoI GCGC 2 cut(s) 314, 475
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 514
Cfr42I CCGCGG 2 cut(s) 108, 225
Cfr9I CCCGGG 1 cut(s) 67
CseI GACGC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 523, 670
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 3 cut(s) 77, 374, 386
CviQI GTAC 2 cut(s) 523, 670
DpnI GATC 4 cut(s) 200, 302, 356, 654
DpnII GATC 4 cut(s) 198, 300, 354, 652
Eam1104I CTCTTC 2 cut(s) 68, 341
EarI CTCTTC 2 cut(s) 68, 341
EciI GGCGGA 2 cut(s) 138, 169
Eco130I CCWWGG 1 cut(s) 243
Eco147I AGGCCT 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 514
Eco88I CYCGRG 1 cut(s) 67
Eco91I GGTNACC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 126
EcoRII CCWGG 3 cut(s) 118, 418, 516
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 3 cut(s) 80, 377, 389
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 527
FatI CATG 3 cut(s) 76, 373, 385
FauI CCCGC 1 cut(s) 100
FauNDI CATATG 1 cut(s) 194
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 2 cut(s) 54, 222
FokI GGATG 1 cut(s) 5
Fsp4HI GCNGC 2 cut(s) 54, 222
FspBI CTAG 3 cut(s) 50, 173, 545
GlaI GCGC 2 cut(s) 313, 474
GluI GCNGC 2 cut(s) 54, 222
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 2 cut(s) 60, 677
HapII CCGG 4 cut(s) 68, 273, 360, 494
HgaI GACGC 1 cut(s) 226
HhaI GCGC 2 cut(s) 314, 475
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 3 cut(s) 80, 377, 389
Hin6I GCGC 2 cut(s) 312, 473
HinP1I GCGC 2 cut(s) 312, 473
HincII GTYRAC 3 cut(s) 85, 292, 381
HindII GTYRAC 3 cut(s) 85, 292, 381
HinfI GANTC 1 cut(s) 377
HpaII CCGG 4 cut(s) 68, 273, 360, 494
HphI GGTGA 4 cut(s) 5, 88, 262, 349
Hpy166II GTNNAC 3 cut(s) 85, 292, 381
Hpy188I TCNGA 1 cut(s) 149
Hpy188III TCNNGA 1 cut(s) 545
Hpy8I GTNNAC 3 cut(s) 85, 292, 381
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 2 cut(s) 306, 528
HpyCH4V TGCA 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 3 cut(s) 391, 470, 614
HpySE526I ACGT 2 cut(s) 306, 528
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 3 cut(s) 80, 377, 389
HspAI GCGC 2 cut(s) 312, 473
Ksp22I TGATCA 1 cut(s) 652
KspI CCGCGG 2 cut(s) 108, 225
Kzo9I GATC 4 cut(s) 198, 300, 354, 652
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 65
MaeI CTAG 3 cut(s) 50, 173, 545
MaeII ACGT 2 cut(s) 306, 528
MaeIII GTNAC 4 cut(s) 94, 247, 329, 529
MalI GATC 4 cut(s) 200, 302, 356, 654
MboI GATC 4 cut(s) 198, 300, 354, 652
MboII GAAGA 3 cut(s) 55, 358, 655
MflI RGATCY 2 cut(s) 198, 300
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 5 cut(s) 8, 126, 255, 438, 619
MlyI GAGTC 1 cut(s) 386
MnlI CCTC 7 cut(s) 38, 71, 342, 427, 447, 594, 680
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 2 cut(s) 275, 362
MspA1I CMGCKG 2 cut(s) 107, 224
MspI CCGG 4 cut(s) 68, 273, 360, 494
MspR9I CCNGG 7 cut(s) 68, 69, 120, 274, 420, 495, 518
MvaI CCWGG 3 cut(s) 120, 420, 518
MvnI CGCG 3 cut(s) 107, 224, 473
MwoI GCNNNNNNNGC 3 cut(s) 391, 470, 614
NciI CCSGG 4 cut(s) 68, 69, 274, 495
NdeI CATATG 1 cut(s) 194
NdeII GATC 4 cut(s) 198, 300, 354, 652
NlaIII CATG 3 cut(s) 80, 377, 389
NlaIV GGNNCC 3 cut(s) 73, 115, 515
NmuCI GTSAC 2 cut(s) 94, 529
NspI RCATGY 1 cut(s) 389
OliI CACNNNNGTG 2 cut(s) 275, 362
PasI CCCWGGG 1 cut(s) 419
PceI AGGCCT 1 cut(s) 60
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 2 cut(s) 55, 223
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PsiI TTATAA 1 cut(s) 612
Psp6I CCWGG 3 cut(s) 118, 418, 516
PspEI GGTNACC 1 cut(s) 247
PspGI CCWGG 3 cut(s) 118, 418, 516
PspN4I GGNNCC 3 cut(s) 73, 115, 515
PspPI GGNCC 1 cut(s) 514
PsuI RGATCY 2 cut(s) 198, 300
RsaI GTAC 2 cut(s) 524, 671
RsaNI GTAC 2 cut(s) 523, 670
RseI CAYNNNNRTG 2 cut(s) 275, 362
SacII CCGCGG 2 cut(s) 108, 225
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 2 cut(s) 54, 222
Sau3AI GATC 4 cut(s) 198, 300, 354, 652
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 7 cut(s) 68, 69, 120, 274, 420, 495, 518
SduI GDGCHC 1 cut(s) 74
SfcI CTRYAG 1 cut(s) 157
Sfr303I CCGCGG 2 cut(s) 108, 225
SgrAI CRCCGGYG 1 cut(s) 359
SgrBI CCGCGG 2 cut(s) 108, 225
SinI GGWCC 1 cut(s) 514
SmaI CCCGGG 1 cut(s) 69
SmiMI CAYNNNNRTG 2 cut(s) 275, 362
Sse9I AATT 5 cut(s) 8, 126, 255, 438, 619
SseBI AGGCCT 1 cut(s) 60
SsiI CCGC 6 cut(s) 105, 107, 123, 180, 222, 224
SspMI CTAG 3 cut(s) 50, 173, 545
StuI AGGCCT 1 cut(s) 60
StyD4I CCNGG 7 cut(s) 66, 67, 118, 272, 418, 493, 516
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 2 cut(s) 309, 531
TasI AATT 5 cut(s) 8, 126, 255, 438, 619
TatI WGTACW 1 cut(s) 669
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 2 cut(s) 94, 529
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 2 cut(s) 94, 529
TspDTI ATGAA 2 cut(s) 181, 602
TspGWI ACGGA 1 cut(s) 11
TspMI CCCGGG 1 cut(s) 67
TspRI CASTG 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 514
XapI RAATTY 1 cut(s) 126
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 1 cut(s) 389
XmaI CCCGGG 1 cut(s) 67
XspI CTAG 3 cut(s) 50, 173, 545
ZraI GACGTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.