pycom05g09020

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003578
Physical Location & Seq
Reverse (-)
12040999 .. 12041676
678 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09020.1

Sequence Viewer

Length: 678 bp
ATGGTTCACCATATGTATTTCCTCTTGCTCATAACACTAGCAGGCCTTCATGGCACTGCCCATGCAGTCGACTACGACGTCACCAACAATGCCGGAACCAGCGCCGGTGGGGTTCGATTCGATAACGATATCGGAGCCGAGTACAGTAGGCAAACCCTAATATCTGCCACTGACTTCATACGGAGGTTGTTCCAGCAAAACACAGCCGCTGATGGAAAGAGTGTCCAAAAGATCAGTTTGGTAATTGAAAACATGAGTGGGGTCCCATATACTAGCAACGATGTGATCCACGTTAGCGCCGACTACATAGCTGGTTACCCCGGCGATGTGAAGAGAGAGATCACCGGTGTGCTTTACCATGAGACAACGCACGTGTGGCAATGGAATGGGAATGGTCAAGCCCCAGGGGGTTTGATTGAGGGAATTGCTGATTACGTGAGGTTGAAGGCAGGGTACGACCCGAGTCATTGGGTTCAGGCAGGGCAGGGGGATAGGTGGGACAAGGGATACGATGTTACTGCTAGGTTTTTGGACTATTTGAATGGTTTGAGAGATGGGTTCGTGTCTGAATTGAATAGCAAATTAAGGGATGGCTATAGTGCTACATATTTTGTTGACTTGCTGGGGAAGAATGTTGATCAGCTTTGGACGGAATACAAGGCCAAGTATGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

226

Amino Acids

24.99

Weight (kDa)

5.53

Isoelectric Point (pI)

25.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 22 - 219 7.1e-73 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 207
AclWI GGATC 1 cut(s) 280
AcvI CACGTG 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 2 cut(s) 143, 455
AflIII ACRYGT 1 cut(s) 372
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 4 cut(s) 248, 445, 541, 574
AjnI CCWGG 1 cut(s) 403
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 2 cut(s) 311, 643
AluI AGCT 2 cut(s) 311, 643
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 2 cut(s) 43, 660
AsiGI ACCGGT 1 cut(s) 344
AspLEI GCGC 2 cut(s) 104, 299
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 73, 334
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 1 cut(s) 262
BarI GAAGNNNNNNTAC 2 cut(s) 437, 469
BbrPI CACGTG 1 cut(s) 373
BccI CCATC 3 cut(s) 206, 548, 584
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 500
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 1 cut(s) 321
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 3 cut(s) 38, 273, 522
BfmI CTRYAG 1 cut(s) 595
BfoI RGCGCY 2 cut(s) 105, 300
BfuI GTATCC 1 cut(s) 500
BglI GCCNNNNNGGC 1 cut(s) 51
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 2 cut(s) 321, 405
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 4 cut(s) 97, 136, 263, 264
BmrFI CCNGG 2 cut(s) 321, 405
BoxI GACNNNNGTC 1 cut(s) 462
BpuMI CCSGG 1 cut(s) 321
BsaAI YACGTR 2 cut(s) 373, 436
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 319, 403, 404
BsaWI WCCGGW 1 cut(s) 344
Bse118I RCCGGY 2 cut(s) 104, 344
Bse3DI GCAATG 1 cut(s) 386
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 319, 403, 404
BseGI GGATG 1 cut(s) 595
BseMI GCAATG 1 cut(s) 386
BseYI CCCAGC 1 cut(s) 622
BshFI GGCC 2 cut(s) 45, 662
BshTI ACCGGT 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 4 cut(s) 93, 105, 321, 345
BslFI GGGAC 2 cut(s) 248, 512
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 248, 512
BsnI GGCC 2 cut(s) 45, 662
BsoBI CYCGRG 1 cut(s) 460
Bsp143I GATC 4 cut(s) 231, 285, 339, 637
BspACI CCGC 1 cut(s) 207
BspANI GGCC 2 cut(s) 45, 662
BspLI GGNNCC 4 cut(s) 97, 136, 263, 264
BspPI GGATC 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 386
BsrFI RCCGGY 2 cut(s) 104, 344
BssAI RCCGGY 2 cut(s) 104, 344
BssECI CCNNGG 3 cut(s) 319, 403, 404
BssMI GATC 4 cut(s) 231, 285, 339, 637
BssNI GRCGYC 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 78
BstBAI YACGTR 2 cut(s) 373, 436
BstC8I GCNNGC 1 cut(s) 43
BstEII GGTNACC 1 cut(s) 314
BstF5I GGATG 1 cut(s) 595
BstH2I RGCGCY 2 cut(s) 105, 300
BstHHI GCGC 2 cut(s) 104, 299
BstKTI GATC 4 cut(s) 234, 288, 342, 640
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 4 cut(s) 231, 285, 339, 637
BstMWI GCNNNNNNNGC 3 cut(s) 51, 376, 668
BstNI CCWGG 1 cut(s) 405
BstPAI GACNNNNGTC 1 cut(s) 462
BstPI GGTNACC 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 319, 403
BstSFI CTRYAG 1 cut(s) 595
BsuI GTATCC 1 cut(s) 500
BsuRI GGCC 2 cut(s) 45, 662
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 595
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 168
Cac8I GCNNGC 1 cut(s) 43
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 2 cut(s) 104, 299
Cfr10I RCCGGY 2 cut(s) 104, 344
Cfr13I GGNCC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 142, 454
CspAI ACCGGT 1 cut(s) 344
CviAII CATG 4 cut(s) 50, 62, 253, 359
CviJI RGCY 8 cut(s) 45, 137, 206, 311, 401, 594, 643, 662
CviKI_1 RGCY 8 cut(s) 45, 137, 206, 311, 401, 594, 643, 662
CviQI GTAC 2 cut(s) 142, 454
DpnI GATC 4 cut(s) 233, 287, 341, 639
DpnII GATC 4 cut(s) 231, 285, 339, 637
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco147I AGGCCT 1 cut(s) 45
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 262
Eco72I CACGTG 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 460
Eco91I GGTNACC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 1 cut(s) 130
FaeI CATG 4 cut(s) 53, 65, 256, 362
FaqI GGGAC 2 cut(s) 248, 512
FatI CATG 4 cut(s) 49, 61, 252, 358
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 3 cut(s) 38, 273, 522
GlaI GCGC 2 cut(s) 103, 298
GluI GCNGC 1 cut(s) 207
GsaI CCCAGC 1 cut(s) 626
HaeII RGCGCY 2 cut(s) 105, 300
HaeIII GGCC 2 cut(s) 45, 662
HapII CCGG 4 cut(s) 93, 105, 321, 345
HhaI GCGC 2 cut(s) 104, 299
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 4 cut(s) 53, 65, 256, 362
Hin6I GCGC 2 cut(s) 102, 297
HinP1I GCGC 2 cut(s) 102, 297
HincII GTYRAC 2 cut(s) 70, 616
HindII GTYRAC 2 cut(s) 70, 616
HinfI GANTC 2 cut(s) 117, 463
HpaII CCGG 4 cut(s) 93, 105, 321, 345
HphI GGTGA 2 cut(s) 73, 334
Hpy166II GTNNAC 3 cut(s) 7, 70, 616
Hpy188I TCNGA 2 cut(s) 134, 568
Hpy8I GTNNAC 3 cut(s) 7, 70, 616
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 56, 439
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 4 cut(s) 78, 291, 372, 435
HpyCH4V TGCA 2 cut(s) 65, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 376, 668
HpySE526I ACGT 4 cut(s) 78, 291, 372, 435
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 4 cut(s) 53, 65, 256, 362
HspAI GCGC 2 cut(s) 102, 297
KflI GGGWCCC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 4 cut(s) 231, 285, 339, 637
LmnI GCTCC 1 cut(s) 134
MaeI CTAG 3 cut(s) 38, 273, 522
MaeII ACGT 4 cut(s) 78, 291, 372, 435
MaeIII GTNAC 3 cut(s) 79, 314, 514
MalI GATC 4 cut(s) 233, 287, 341, 639
MboI GATC 4 cut(s) 231, 285, 339, 637
MboII GAAGA 2 cut(s) 343, 640
MluCI AATT 4 cut(s) 243, 423, 569, 581
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 4 cut(s) 32, 177, 412, 432
MseI TTAA 1 cut(s) 584
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 4 cut(s) 93, 105, 321, 345
MspR9I CCNGG 2 cut(s) 321, 405
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 3 cut(s) 51, 376, 668
NciI CCSGG 1 cut(s) 321
NdeI CATATG 1 cut(s) 12
NdeII GATC 4 cut(s) 231, 285, 339, 637
NlaIII CATG 4 cut(s) 53, 65, 256, 362
NlaIV GGNNCC 4 cut(s) 97, 136, 263, 264
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 347
PasI CCCWGGG 1 cut(s) 404
PceI AGGCCT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 297
PfeI GAWTC 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 1 cut(s) 471
PmaCI CACGTG 1 cut(s) 373
PmlI CACGTG 1 cut(s) 373
PpsI GAGTC 1 cut(s) 471
Ppu21I YACGTR 2 cut(s) 373, 436
PpuMI RGGWCCY 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 403
PspCI CACGTG 1 cut(s) 373
PspEI GGTNACC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 622
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 4 cut(s) 97, 136, 263, 264
PspPI GGNCC 1 cut(s) 262
PspPPI RGGWCCY 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 143, 455
RsaNI GTAC 2 cut(s) 142, 454
RseI CAYNNNNRTG 1 cut(s) 347
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 231, 285, 339, 637
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 2 cut(s) 321, 405
SfcI CTRYAG 1 cut(s) 595
SgrAI CRCCGGYG 2 cut(s) 104, 344
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 347
Sse9I AATT 4 cut(s) 243, 423, 569, 581
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 1 cut(s) 207
SspMI CTAG 3 cut(s) 38, 273, 522
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 319, 403
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 4 cut(s) 81, 294, 375, 438
TaqI TCGA 3 cut(s) 69, 115, 120
TasI AATT 4 cut(s) 243, 423, 569, 581
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 2 cut(s) 61, 175
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 38, 166
TspGWI ACGGA 2 cut(s) 196, 665
TspRI CASTG 2 cut(s) 61, 175
VpaK11BI GGWCC 1 cut(s) 262
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 3 cut(s) 38, 273, 522
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.