FvH4_2g19100

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005665
Physical Location & Seq
Reverse (-)
16291894 .. 16295679
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19100.t1

Sequence Viewer

Length: 789 bp
ATGGAGAATCATCACATTTCCGACGCCGACCGCGAGCCGGAGGATTTCTCTGAGCGCTTCCTCTGCTGCGTCTGCCTGGATCTTCTGTACAAGCCTATAGTGCTCTCGTGTGGCCACATTACGTGTTTCTGGTGCGTGCATCGATCCATGAATGAAGAAGAGGCGGAAACTGGCGTCTTCTCACCACAGCTGGATGTTCAAGCTTGGAGCTCACAAGCTAATCAAGAATCGAATGTTCTGGGGTACTCTATTAGCAAAGAGTTGACTGCAAATATGGAGACAACAGGTTCTGTTTCATATGATCAGAACGATGGGACAGCTGTCTCTAGGCCAGCTGATGGTGGAGACTATGAGGGAAAGGAGAATGTTACTGATCATGTGAAGAACTCCCTGTTAAGTCATCAGCAGAGTGGAAGCTCCAAGCAGATTTCAGTTGCAGATGTGCTATGTGCTGCATGCAAAGATCTAGTGTTTCGTCCTGTGGTTCTCAACTGTGGCCATGTATATTGTGAAAGTTGTATGTCAACCCAGATGAACAGAAGCTTAGATGTCAAGTTTGTCCAAGTTTCCATCCAACTGGATGTCCAAAAGTTTGTTTGGAACTGCACAACTTTTTGGTCGAACAGTTTCCCAAAGAACTTGCACATAGAAGAGATGATGTACAGCTTAAGCATGTTAATCCAACTGCATGTGAAAGCAAAAGCAAAAGCAGTAAAGAAGCAGAGAGGCCATCACCGTGGTCATATCACTCAGGAGTTGGCTGCGATTATTGTGGAATGTGTCCAATAA

Protein Analysis

263

Amino Acids

29.4

Weight (kDa)

5.6

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_4 PF15227 22 - 56 4.3e-08 zinc finger of C3HC4-type, RING
zf-C3HC4_2 PF13923 22 - 54 6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 150 - 181 2.8e-06 zinc finger of C3HC4-type, RING
zf-RING_UBOX PF13445 150 - 180 5.6e-07 RING-type zinc-finger
zf-C3HC4 PF00097 150 - 179 3.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 338
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 31, 164
AclWI GGATC 2 cut(s) 87, 138
AcoI YGGCCR 2 cut(s) 112, 496
AcyI GRCGYC 2 cut(s) 24, 174
AfaI GTAC 3 cut(s) 89, 245, 662
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 37, 338
AflII CTTAAG 1 cut(s) 667
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 9 cut(s) 190, 203, 210, 218, 320, 335, 417, 543, 666
AluI AGCT 9 cut(s) 190, 203, 210, 218, 320, 335, 417, 543, 666
Alw21I GWGCWC 2 cut(s) 105, 212
Alw26I GTCTC 3 cut(s) 272, 328, 339
AlwI GGATC 2 cut(s) 87, 138
AlwNI CAGNNNCTG 1 cut(s) 290
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 4 cut(s) 112, 329, 496, 727
ApeKI GCWGC 3 cut(s) 66, 452, 761
Asp700I GAANNNNTTC 1 cut(s) 626
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 174, 725
BalI TGGCCA 2 cut(s) 114, 498
BanII GRGCYC 1 cut(s) 212
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 105, 212
BbvI GCAGC 3 cut(s) 53, 439, 748
BccI CCATC 4 cut(s) 305, 332, 578, 738
BciT130I CCWGG 1 cut(s) 77
BclI TGATCA 2 cut(s) 301, 373
BcoDI GTCTC 3 cut(s) 272, 328, 339
BfaI CTAG 2 cut(s) 327, 467
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 58
BfrI CTTAAG 1 cut(s) 667
BglII AGATCT 1 cut(s) 463
BisI GCNGC 3 cut(s) 67, 453, 762
BlsI GCNGC 3 cut(s) 68, 454, 763
Bme1390I CCNGG 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 148
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 169
BplI GAGNNNNNCTC 2 cut(s) 32, 64
Bsa29I ATCGAT 1 cut(s) 142
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 24, 174
BsaJI CCNNGG 1 cut(s) 736
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 338
Bse1I ACTGG 2 cut(s) 175, 582
BseBI CCWGG 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 3 cut(s) 199, 570, 586
BseLI CCNNNNNNNGG 2 cut(s) 37, 338
BseMII CTCAG 2 cut(s) 42, 764
BseNI ACTGG 2 cut(s) 175, 582
BseXI GCAGC 3 cut(s) 53, 439, 748
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 4 cut(s) 114, 331, 498, 729
BshVI ATCGAT 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 105, 212
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 37, 338
BsmAI GTCTC 3 cut(s) 272, 328, 339
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 4 cut(s) 114, 331, 498, 729
Bsp1286I GDGCHC 2 cut(s) 105, 212
Bsp1407I TGTACA 2 cut(s) 87, 660
Bsp143I GATC 5 cut(s) 79, 143, 301, 373, 463
BspACI CCGC 2 cut(s) 31, 164
BspANI GGCC 4 cut(s) 114, 331, 498, 729
BspCNI CTCAG 2 cut(s) 43, 763
BspDI ATCGAT 1 cut(s) 142
BspFNI CGCG 1 cut(s) 33
BspPI GGATC 2 cut(s) 87, 138
BspTI CTTAAG 1 cut(s) 667
BsrGI TGTACA 2 cut(s) 87, 660
BsrI ACTGG 2 cut(s) 175, 582
BssECI CCNNGG 1 cut(s) 736
BssMI GATC 5 cut(s) 79, 143, 301, 373, 463
BssNI GRCGYC 2 cut(s) 24, 174
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 494, 626, 737
Bst6I CTCTTC 2 cut(s) 153, 645
BstACI GRCGYC 2 cut(s) 24, 174
BstAFI CTTAAG 1 cut(s) 667
BstAUI TGTACA 2 cut(s) 87, 660
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 4 cut(s) 35, 137, 333, 457
BstDEI CTNAG 3 cut(s) 51, 544, 750
BstDSI CCRYGG 1 cut(s) 736
BstF5I GGATG 3 cut(s) 199, 570, 586
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 5 cut(s) 82, 146, 304, 376, 466
BstMAI GTCTC 3 cut(s) 272, 328, 339
BstMBI GATC 5 cut(s) 79, 143, 301, 373, 463
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 3 cut(s) 63, 72, 100
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 3 cut(s) 459, 676, 692
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 53, 439, 748
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 2 cut(s) 79, 463
BstXI CCANNNNNNTGG 2 cut(s) 577, 737
BstYI RGATCY 2 cut(s) 79, 463
Bsu15I ATCGAT 1 cut(s) 142
BsuRI GGCC 4 cut(s) 114, 331, 498, 729
BsuTUI ATCGAT 1 cut(s) 142
BtgI CCRYGG 1 cut(s) 736
BtsCI GGATG 3 cut(s) 199, 570, 586
Cac8I GCNNGC 4 cut(s) 35, 137, 333, 457
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 57
ClaI ATCGAT 1 cut(s) 142
CseI GACGC 3 cut(s) 32, 58, 163
Csp6I GTAC 3 cut(s) 88, 244, 661
CviAII CATG 6 cut(s) 148, 377, 456, 500, 673, 689
CviQI GTAC 3 cut(s) 88, 244, 661
DdeI CTNAG 3 cut(s) 51, 544, 750
DpnI GATC 5 cut(s) 81, 145, 303, 375, 465
DpnII GATC 5 cut(s) 79, 143, 301, 373, 463
EaeI YGGCCR 2 cut(s) 112, 496
Eam1104I CTCTTC 2 cut(s) 153, 645
EarI CTCTTC 2 cut(s) 153, 645
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 212
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 212
FaeI CATG 6 cut(s) 151, 380, 459, 503, 676, 692
FaqI GGGAC 1 cut(s) 328
FatI CATG 6 cut(s) 147, 376, 455, 499, 672, 688
FauNDI CATATG 1 cut(s) 298
FbaI TGATCA 2 cut(s) 301, 373
Fnu4HI GCNGC 3 cut(s) 67, 453, 762
FokI GGATG 3 cut(s) 206, 557, 593
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 3 cut(s) 67, 453, 762
FspBI CTAG 2 cut(s) 327, 467
GlaI GCGC 1 cut(s) 56
GluI GCNGC 3 cut(s) 67, 453, 762
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 4 cut(s) 114, 331, 498, 729
HapII CCGG 1 cut(s) 38
HgaI GACGC 3 cut(s) 32, 58, 163
HhaI GCGC 1 cut(s) 57
Hin1I GRCGYC 2 cut(s) 24, 174
Hin1II CATG 6 cut(s) 151, 380, 459, 503, 676, 692
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 2 cut(s) 264, 525
HindII GTYRAC 2 cut(s) 264, 525
HindIII AAGCTT 2 cut(s) 201, 541
HinfI GANTC 2 cut(s) 7, 227
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 174, 725
Hpy166II GTNNAC 2 cut(s) 264, 525
Hpy188I TCNGA 3 cut(s) 22, 52, 306
Hpy188III TCNNGA 2 cut(s) 224, 752
Hpy8I GTNNAC 2 cut(s) 264, 525
Hpy99I CGWCG 1 cut(s) 26
HpyCH4III ACNGT 3 cut(s) 494, 626, 737
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 8 cut(s) 139, 269, 437, 455, 459, 606, 643, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 72, 100
HpyF3I CTNAG 3 cut(s) 51, 544, 750
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 2 cut(s) 24, 174
Hsp92II CATG 6 cut(s) 151, 380, 459, 503, 676, 692
HspAI GCGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 301, 373
Kzo9I GATC 5 cut(s) 79, 143, 301, 373, 463
LmnI GCTCC 2 cut(s) 207, 422
Lsp1109I GCAGC 3 cut(s) 53, 439, 748
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 327, 467
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 1 cut(s) 367
MalI GATC 5 cut(s) 81, 145, 303, 375, 465
MboI GATC 5 cut(s) 79, 143, 301, 373, 463
MboII GAAGA 6 cut(s) 74, 167, 169, 170, 394, 662
MflI RGATCY 2 cut(s) 79, 463
MhlI GDGCHC 2 cut(s) 105, 212
MlsI TGGCCA 2 cut(s) 114, 498
MluNI TGGCCA 2 cut(s) 114, 498
MmeI TCCRAC 3 cut(s) 45, 598, 706
MnlI CCTC 5 cut(s) 34, 71, 154, 346, 719
Mox20I TGGCCA 2 cut(s) 114, 498
MroXI GAANNNNTTC 1 cut(s) 626
MscI TGGCCA 2 cut(s) 114, 498
MseI TTAA 3 cut(s) 395, 668, 677
MslI CAYNNNNRTG 1 cut(s) 735
Msp20I TGGCCA 2 cut(s) 114, 498
MspA1I CMGCKG 3 cut(s) 190, 320, 335
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 63, 72, 100
NdeI CATATG 1 cut(s) 298
NdeII GATC 5 cut(s) 79, 143, 301, 373, 463
NlaIII CATG 6 cut(s) 151, 380, 459, 503, 676, 692
NspI RCATGY 3 cut(s) 459, 676, 692
PaeI GCATGC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 626
PfeI GAWTC 2 cut(s) 7, 227
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 3 cut(s) 68, 454, 763
Ppu21I YACGTR 1 cut(s) 123
PshAI GACNNNNGTC 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 2 cut(s) 79, 463
PvuII CAGCTG 3 cut(s) 190, 320, 335
RsaI GTAC 3 cut(s) 89, 245, 662
RsaNI GTAC 3 cut(s) 88, 244, 661
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 395, 668, 677
SatI GCNGC 3 cut(s) 67, 453, 762
Sau3AI GATC 5 cut(s) 79, 143, 301, 373, 463
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 2 cut(s) 105, 212
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
SphI GCATGC 1 cut(s) 459
SsiI CCGC 2 cut(s) 31, 164
SspMI CTAG 2 cut(s) 327, 467
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 75
TaaI ACNGT 3 cut(s) 494, 626, 737
TaiI ACGT 1 cut(s) 125
TaqI TCGA 3 cut(s) 142, 230, 620
TatI WGTACW 2 cut(s) 87, 660
TfiI GAWTC 2 cut(s) 7, 227
Tru1I TTAA 3 cut(s) 395, 668, 677
Tru9I TTAA 3 cut(s) 395, 668, 677
TseI GCWGC 3 cut(s) 66, 452, 761
TspDTI ATGAA 4 cut(s) 164, 168, 285, 548
Van91I CCANNNNNTGG 1 cut(s) 338
Vha464I CTTAAG 1 cut(s) 667
XceI RCATGY 3 cut(s) 459, 676, 692
XmnI GAANNNNTTC 1 cut(s) 626
XspI CTAG 2 cut(s) 327, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.