Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0003242

Physical Location & Seq

Genomic Coordinates Forward (+)

18436880 .. 18439572

Gene Structure 2693 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g22480.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGAGCCCATCTCTCTGGACCAAAAGGGAGGTTCCTGATGAGCCTGCGTTTATGCAAGCAAATGATGAGAATGGAAATGGGAAAGAGATGAGTTTGGAGGTTATGAGGGTGAACAAGAGGAAGAAACCGCGCAATGAGGTTGATGAGAAAGACGCGGTGGAGCAAGAGAAGGAGATGAAGAAGCTGGAGAGTTTTTTGTTTGGAAACATTTATGCGCCGGTGGAGTTTGGGAAGGAGGTTGAGGAGGGAGATGCAGTGGATAATGACTCTTCTTTGTTCTTTGTGGATCGTTCTGCAAATAGTGTGCTTTCTGTTGGTGAAGATGATGGAGAAGTATTGGAGGAGGAACCTAAGCAAAGGAAAGCTGTCTGGGAGGATGATGAAGAGGCAAAGGCCAATGTGAATATTGCCAAAGTTAATAGATTAAGGAAGCTGAGGAAGGAAGAGGATGAGAGTTTGATTTCTGGTTCTGAGTATGTTTCAAGATTGAGGGCGCAACATGTTAAGCTGAATCCCGGCACGGAGTGGGCTCAACTTGATTCAGAGCCAAGGAACAATAAATATTCTGACGATGAATCATCAGATGATGAAGATAGGGCAGTAGTGGCCCATGGCTACAAGGCTGTTGGAGCTGTTGATGATCTCCTTCGAACAAATGAGGATTTTGTTGTGAAGAGCAGTTCAAAGTTGTTGCCTGGGCTTCTTGAATACTCAATACTGACAGATGCTAACGCAGAGGAACCTTCTAGTGGTCCTATAAATTCAGTGCAGTTTCATAGAAATGCTCAGTTGCTTCTGACTGCTGGATTGGATCGAAGGCTTAGGTTTTTCCAGATTGATGGAAAACGGAATGCTAAAATTGAAAGCATCTTCATTGATGATTGTCCAATTCGGAAGGCAGCCTTCTTGCCTGATGGCTCTCAGGTTATTATAGCTGGAAGGAGGAAGTTCTTTTACAGCTTTGATTTAGTGAAGGCCAAAGTTGATAGAATAGGACCCCTTGTTGGTAGAGAGGAGAAAAGCTTGGAAGTTTTTGAGGTTTCCCCTGATTCTAGTACAATTGCCTTTGTGGGCAATGAAGGGTATATCTTGTTGGTTTCCACTAAAACAAAGGAATTGATTGGGACACTGAAGATGAATGGAACTGCACGTTCTTTAGCTTTTTCCGAAGATGGGAAGCAGTTATTGAGCTCTGGTGGAGATGGACAGATTTACCACTGGGATTTGAGAACAGGAGCTTGCTTCCATAAGGCTGTTGATGAAGGCTGTATAAATGGCACATCTCTGTGTACATCTCCAAATGGAAAACTGTTTGCTGCTGGTTCAGACAGTGGGATTGTCAATATCTACAACAGAGAAGAATTTTTAGGGGGCAAGAGAAAACCCATCAAGACAGTCGAGAATCTCACAACCAAAGTAGATTTTCTCAAGTTCAATACCGATTCTCAAATACTGGCTATTTGTTCAAGCATGAAGAAGAACAGCTTGAAGTTGGTACATGTTCCATCATTGACTGTGTTCTCTAACTGGCCTCCACAGAAGAAGACCCTGCACTATCCCCGGTGTTTGGATTTCAGTCCTGGTGGAGGTTTCATGGCCATGGGAAATGCTGCTGGAAAAGTTTTGCTGTACAAGTTGCACCACTACCATGATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

553

Amino Acids

61.49

Weight (kDa)

5.55

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Prp19	PF24814	249 - 489	2.4e-19	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	249 - 417	5.4e-06	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	254 - 417	3.9e-09	WDHD1 first WD40 domain
WD40_Gbeta	PF25391	255 - 450	2.9e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	257 - 414	8.7e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	300 - 547	1e-11	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	322 - 506	4.6e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	332 - 416	2.7e-07	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	338 - 416	9.4e-06	WDR3 first beta-propeller domain
WDR55	PF24796	345 - 516	8.9e-09	WDR55
Beta-prop_THOC3	PF25174	346 - 533	3.9e-19	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	347 - 490	8.2e-13	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14050
fragaria_vesca	FvH4_2g22480 FvH4_2g22480 FvH4_5g20520
malus_domestica	MD05G1205300.v1.1 MD05G1205400.v1.1 MD10G1192400.v1.1
prunus_persica	Prupe.5G054300_v2.0.a1 Prupe.5G054300_v2.0.a1 Prupe.8G231800_v2.0.a1
pyrus_communis	pycom05g19150 pycom12426g00550 pycom13g23680
rosa_chinensis	RchiOBHm_Chr6g0289431 RchiOBHm_Chr7g0205871 RchiOBHm_Chr7g0205881
rosa_laevigata	RLG00000012303
rosa_multiflora	Rmu_sc0000076.1_g000034 Rmu_sc0001527.1_g000035 Rmu_sc0004084.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251900 Rroxscaffold_7G00179220
rosa_rugosa	Rorug06G0203300 Rorug06G0203400 Rorug07G0093600
rosa_samantha	Rh6AG314600 Rh6BG322100 Rh6CG328300 Rh6DG314000 Rh7AG225300 Rh7BG221200 Rh7CG238900 Rh7CG239300 Rh7CG239400 Rh7DG232000
rosa_wichuraiana	Rw6G027180 Rw7G019430

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	130, 155
AciI	CCGC	2 cut(s)	128, 155
AclWI	GGATC	2 cut(s)	294, 819
AcoI	YGGCCR	1 cut(s)	1596
AcsI	RAATTY	2 cut(s)	760, 1361
AcuI	CTGAAG	1 cut(s)	1151
AdeI	CACNNNGTG	1 cut(s)	525
AfaI	GTAC	4 cut(s)	1057, 1291, 1497, 1631
AfiI	CCNNNNNNNGG	5 cut(s)	749, 1004, 1173, 1567, 1586
AflIII	ACRYGT	2 cut(s)	499, 1498
AgsI	TTSAA	7 cut(s)	483, 684, 707, 863, 1435, 1467, 1489
AjnI	CCWGG	2 cut(s)	694, 1579
Alw21I	GWGCWC	1 cut(s)	1193
AlwI	GGATC	2 cut(s)	294, 819
AoxI	GGCC	5 cut(s)	393, 606, 975, 1529, 1596
ApeKI	GCWGC	3 cut(s)	899, 1316, 1610
ApoI	RAATTY	2 cut(s)	760, 1361
AspLEI	GCGC	3 cut(s)	132, 217, 496
AspS9I	GGNCC	4 cut(s)	18, 607, 752, 995
AsuC2I	CCSGG	2 cut(s)	516, 1561
AsuHPI	GGTGA	2 cut(s)	121, 329
AsuII	TTCGAA	1 cut(s)	649
AvaII	GGWCC	3 cut(s)	18, 752, 995
BalI	TGGCCA	1 cut(s)	1598
BanII	GRGCYC	3 cut(s)	8, 532, 1193
BarI	GAAGNNNNNNTAC	2 cut(s)	938, 970
BbsI	GAAGAC	1 cut(s)	1550
Bbv12I	GWGCWC	1 cut(s)	1193
BbvCI	CCTCAGC	1 cut(s)	434
BbvI	GCAGC	3 cut(s)	911, 1303, 1597
BccI	CCATC	8 cut(s)	16, 320, 833, 908, 1166, 1196, 1394, 1513
BciT130I	CCWGG	2 cut(s)	696, 1581
BcnI	CCSGG	2 cut(s)	516, 1561
BfaI	CTAG	2 cut(s)	747, 1053
BisI	GCNGC	3 cut(s)	900, 1317, 1611
BlsI	GCNGC	3 cut(s)	901, 1318, 1612
Bme1390I	CCNGG	4 cut(s)	516, 696, 1561, 1581
Bme18I	GGWCC	3 cut(s)	18, 752, 995
BmgT120I	GGNCC	4 cut(s)	18, 607, 752, 995
BmiI	GGNNCC	4 cut(s)	33, 348, 741, 997
BmrFI	CCNGG	4 cut(s)	516, 696, 1561, 1581
BmrI	ACTGGG	1 cut(s)	1228
BmsI	GCATC	4 cut(s)	241, 715, 876, 1642
BmuI	ACTGGG	1 cut(s)	1228
BpiI	GAAGAC	1 cut(s)	1550
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	206
Bpu10I	CCTNAGC	3 cut(s)	351, 434, 821
Bpu14I	TTCGAA	1 cut(s)	649
BpuEI	CTTGAG	1 cut(s)	1412
BpuMI	CCSGG	2 cut(s)	516, 1561
BsaJI	CCNNGG	5 cut(s)	548, 610, 695, 1559, 1599
BsaXI	ACNNNNNCTCC	2 cut(s)	240, 270
Bsc4I	CCNNNNNNNGG	5 cut(s)	749, 1004, 1173, 1567, 1586
Bse118I	RCCGGY	1 cut(s)	217
Bse1I	ACTGG	3 cut(s)	1223, 1458, 1532
Bse3DI	GCAATG	2 cut(s)	139, 1081
BseBI	CCWGG	2 cut(s)	696, 1581
BseDI	CCNNGG	5 cut(s)	548, 610, 695, 1559, 1599
BseGI	GGATG	2 cut(s)	382, 454
BseLI	CCNNNNNNNGG	5 cut(s)	749, 1004, 1173, 1567, 1586
BseMI	GCAATG	2 cut(s)	139, 1081
BseMII	CTCAG	4 cut(s)	425, 462, 800, 935
BseNI	ACTGG	3 cut(s)	1223, 1458, 1532
BseRI	GAGGAG	3 cut(s)	257, 356, 1028
BseXI	GCAGC	3 cut(s)	911, 1303, 1597
BsgI	GTGCAG	3 cut(s)	788, 1131, 1535
Bsh1236I	CGCG	2 cut(s)	130, 155
BshFI	GGCC	5 cut(s)	395, 608, 977, 1531, 1598
BsiHKAI	GWGCWC	1 cut(s)	1193
BsiSI	CCGG	3 cut(s)	218, 516, 1561
BslFI	GGGAC	1 cut(s)	1138
BslI	CCNNNNNNNGG	5 cut(s)	749, 1004, 1173, 1567, 1586
BsmFI	GGGAC	1 cut(s)	1138
BsmI	GAATGC	1 cut(s)	856
BsnI	GGCC	5 cut(s)	395, 608, 977, 1531, 1598
Bsp119I	TTCGAA	1 cut(s)	649
Bsp1286I	GDGCHC	3 cut(s)	8, 532, 1193
Bsp1407I	TGTACA	2 cut(s)	1289, 1629
Bsp143I	GATC	3 cut(s)	286, 640, 811
Bsp19I	CCATGG	2 cut(s)	610, 1599
BspACI	CCGC	2 cut(s)	128, 155
BspANI	GGCC	5 cut(s)	395, 608, 977, 1531, 1598
BspCNI	CTCAG	4 cut(s)	426, 463, 799, 934
BspFNI	CGCG	2 cut(s)	130, 155
BspLI	GGNNCC	4 cut(s)	33, 348, 741, 997
BspPI	GGATC	2 cut(s)	294, 819
BspQI	GCTCTTC	1 cut(s)	668
BspT104I	TTCGAA	1 cut(s)	649
BsrDI	GCAATG	2 cut(s)	139, 1081
BsrFI	RCCGGY	1 cut(s)	217
BsrGI	TGTACA	2 cut(s)	1289, 1629
BsrI	ACTGG	3 cut(s)	1223, 1458, 1532
BssAI	RCCGGY	1 cut(s)	217
BssECI	CCNNGG	5 cut(s)	548, 610, 695, 1559, 1599
BssMI	GATC	3 cut(s)	286, 640, 811
BssT1I	CCWWGG	3 cut(s)	548, 610, 1599
Bst2UI	CCWGG	2 cut(s)	696, 1581
Bst4CI	ACNGT	4 cut(s)	1311, 1331, 1396, 1516
Bst6I	CTCTTC	4 cut(s)	274, 378, 438, 668
BstAUI	TGTACA	2 cut(s)	1289, 1629
BstBI	TTCGAA	1 cut(s)	649
BstC8I	GCNNGC	3 cut(s)	45, 57, 1240
BstDEI	CTNAG	6 cut(s)	351, 434, 471, 786, 821, 921
BstDSI	CCRYGG	2 cut(s)	610, 1599
BstENI	CCTNNNNNAGG	1 cut(s)	1584
BstF5I	GGATG	2 cut(s)	382, 454
BstFNI	CGCG	2 cut(s)	130, 155
BstHHI	GCGC	3 cut(s)	132, 217, 496
BstKTI	GATC	3 cut(s)	289, 643, 814
BstMBI	GATC	3 cut(s)	286, 640, 811
BstMWI	GCNNNNNNNGC	2 cut(s)	605, 629
BstNI	CCWGG	2 cut(s)	696, 1581
BstNSI	RCATGY	2 cut(s)	503, 1502
BstSCI	CCNGG	4 cut(s)	514, 694, 1559, 1579
BstUI	CGCG	2 cut(s)	130, 155
BstV1I	GCAGC	3 cut(s)	911, 1303, 1597
BstV2I	GAAGAC	1 cut(s)	1550
BstXI	CCANNNNNNTGG	2 cut(s)	15, 839
BsuRI	GGCC	5 cut(s)	395, 608, 977, 1531, 1598
BtgI	CCRYGG	2 cut(s)	610, 1599
BtsCI	GGATG	2 cut(s)	382, 454
BtsI	GCAGTG	1 cut(s)	261
BtsIMutI	CAGTG	5 cut(s)	261, 771, 1127, 1216, 1336
Cac8I	GCNNGC	3 cut(s)	45, 57, 1240
CfoI	GCGC	3 cut(s)	132, 217, 496
Cfr10I	RCCGGY	1 cut(s)	217
Cfr13I	GGNCC	4 cut(s)	18, 607, 752, 995
CseI	GACGC	1 cut(s)	161
Csp6I	GTAC	4 cut(s)	1056, 1290, 1496, 1630
CviAII	CATG	7 cut(s)	500, 611, 1471, 1499, 1594, 1600, 1649
CviQI	GTAC	4 cut(s)	1056, 1290, 1496, 1630
DdeI	CTNAG	6 cut(s)	351, 434, 471, 786, 821, 921
DpnI	GATC	3 cut(s)	288, 642, 813
DpnII	GATC	3 cut(s)	286, 640, 811
DraIII	CACNNNGTG	1 cut(s)	525
EaeI	YGGCCR	1 cut(s)	1596
Eam1104I	CTCTTC	4 cut(s)	274, 378, 438, 668
EarI	CTCTTC	4 cut(s)	274, 378, 438, 668
Ecl136II	GAGCTC	1 cut(s)	1191
Eco130I	CCWWGG	3 cut(s)	548, 610, 1599
Eco24I	GRGCYC	3 cut(s)	8, 532, 1193
Eco47I	GGWCC	3 cut(s)	18, 752, 995
Eco53kI	GAGCTC	1 cut(s)	1191
Eco57I	CTGAAG	1 cut(s)	1151
EcoICRI	GAGCTC	1 cut(s)	1191
EcoNI	CCTNNNNNAGG	1 cut(s)	1584
EcoO109I	RGGNCCY	1 cut(s)	995
EcoRII	CCWGG	2 cut(s)	694, 1579
EcoT14I	CCWWGG	3 cut(s)	548, 610, 1599
EcoT22I	ATGCAT	1 cut(s)	1657
EcoT38I	GRGCYC	3 cut(s)	8, 532, 1193
ErhI	CCWWGG	3 cut(s)	548, 610, 1599
FaeI	CATG	7 cut(s)	503, 614, 1474, 1502, 1597, 1603, 1652
FalI	AAGNNNNNCTT	4 cut(s)	887, 919, 1469, 1501
FaqI	GGGAC	1 cut(s)	1138
FatI	CATG	7 cut(s)	499, 610, 1470, 1498, 1593, 1599, 1648
Fnu4HI	GCNGC	3 cut(s)	900, 1317, 1611
FokI	GGATG	2 cut(s)	389, 461
FriOI	GRGCYC	3 cut(s)	8, 532, 1193
Fsp4HI	GCNGC	3 cut(s)	900, 1317, 1611
FspBI	CTAG	2 cut(s)	747, 1053
GlaI	GCGC	3 cut(s)	131, 216, 495
GluI	GCNGC	3 cut(s)	900, 1317, 1611
GsuI	CTGGAG	1 cut(s)	206
HaeIII	GGCC	5 cut(s)	395, 608, 977, 1531, 1598
HapII	CCGG	3 cut(s)	218, 516, 1561
HgaI	GACGC	1 cut(s)	161
HhaI	GCGC	3 cut(s)	132, 217, 496
Hin1II	CATG	7 cut(s)	503, 614, 1474, 1502, 1597, 1603, 1652
Hin6I	GCGC	3 cut(s)	130, 215, 494
HinP1I	GCGC	3 cut(s)	130, 215, 494
HindIII	AAGCTT	1 cut(s)	1021
HinfI	GANTC	7 cut(s)	266, 511, 539, 575, 1049, 1402, 1442
HpaII	CCGG	3 cut(s)	218, 516, 1561
HphI	GGTGA	2 cut(s)	121, 329
Hpy166II	GTNNAC	2 cut(s)	112, 1290
Hpy188I	TCNGA	8 cut(s)	472, 544, 568, 583, 798, 894, 1168, 1327
Hpy188III	TCNNGA	7 cut(s)	16, 35, 483, 704, 832, 1390, 1399
Hpy8I	GTNNAC	2 cut(s)	112, 1290
HpyCH4III	ACNGT	4 cut(s)	1311, 1331, 1396, 1516
HpyCH4IV	ACGT	1 cut(s)	1150
HpyCH4V	TGCA	8 cut(s)	55, 254, 296, 769, 1148, 1552, 1639, 1655
HpyF10VI	GCNNNNNNNGC	2 cut(s)	605, 629
HpyF3I	CTNAG	6 cut(s)	351, 434, 471, 786, 821, 921
HpySE526I	ACGT	1 cut(s)	1150
Hsp92II	CATG	7 cut(s)	503, 614, 1474, 1502, 1597, 1603, 1652
HspAI	GCGC	3 cut(s)	130, 215, 494
Kzo9I	GATC	3 cut(s)	286, 640, 811
LguI	GCTCTTC	1 cut(s)	668
LmnI	GCTCC	3 cut(s)	160, 629, 1235
Lsp1109I	GCAGC	3 cut(s)	911, 1303, 1597
LweI	GCATC	4 cut(s)	241, 715, 876, 1642
MaeI	CTAG	2 cut(s)	747, 1053
MaeII	ACGT	1 cut(s)	1150
MalI	GATC	3 cut(s)	288, 642, 813
MboI	GATC	3 cut(s)	286, 640, 811
MfeI	CAATTG	1 cut(s)	1059
MhlI	GDGCHC	3 cut(s)	8, 532, 1193
MlsI	TGGCCA	1 cut(s)	1598
MluCI	AATT	6 cut(s)	760, 858, 888, 1059, 1115, 1361
MluNI	TGGCCA	1 cut(s)	1598
MlyI	GAGTC	1 cut(s)	260
MmeI	TCCRAC	1 cut(s)	607
Mox20I	TGGCCA	1 cut(s)	1598
Mph1103I	ATGCAT	1 cut(s)	1657
MscI	TGGCCA	1 cut(s)	1598
MseI	TTAA	3 cut(s)	417, 425, 504
MslI	CAYNNNNRTG	4 cut(s)	780, 1285, 1598, 1647
Msp20I	TGGCCA	1 cut(s)	1598
MspI	CCGG	3 cut(s)	218, 516, 1561
MspR9I	CCNGG	4 cut(s)	516, 696, 1561, 1581
MunI	CAATTG	1 cut(s)	1059
Mva1269I	GAATGC	1 cut(s)	856
MvaI	CCWGG	2 cut(s)	696, 1581
MvnI	CGCG	2 cut(s)	130, 155
MwoI	GCNNNNNNNGC	2 cut(s)	605, 629
NciI	CCSGG	2 cut(s)	516, 1561
NcoI	CCATGG	2 cut(s)	610, 1599
NdeII	GATC	3 cut(s)	286, 640, 811
NlaIII	CATG	7 cut(s)	503, 614, 1474, 1502, 1597, 1603, 1652
NlaIV	GGNNCC	4 cut(s)	33, 348, 741, 997
NsiI	ATGCAT	1 cut(s)	1657
NspI	RCATGY	2 cut(s)	503, 1502
NspV	TTCGAA	1 cut(s)	649
PciI	ACATGT	2 cut(s)	499, 1498
PciSI	GCTCTTC	1 cut(s)	668
PctI	GAATGC	1 cut(s)	856
PfeI	GAWTC	6 cut(s)	511, 539, 575, 1049, 1402, 1442
PkrI	GCNGC	3 cut(s)	901, 1318, 1612
PleI	GAGTC	1 cut(s)	260
PpsI	GAGTC	1 cut(s)	260
PpuMI	RGGWCCY	1 cut(s)	995
PscI	ACATGT	2 cut(s)	499, 1498
Psp124BI	GAGCTC	1 cut(s)	1193
Psp5II	RGGWCCY	1 cut(s)	995
Psp6I	CCWGG	2 cut(s)	694, 1579
PspGI	CCWGG	2 cut(s)	694, 1579
PspN4I	GGNNCC	4 cut(s)	33, 348, 741, 997
PspPI	GGNCC	4 cut(s)	18, 607, 752, 995
PspPPI	RGGWCCY	1 cut(s)	995
RsaI	GTAC	4 cut(s)	1057, 1291, 1497, 1631
RsaNI	GTAC	4 cut(s)	1056, 1290, 1496, 1630
RseI	CAYNNNNRTG	4 cut(s)	780, 1285, 1598, 1647
SacI	GAGCTC	1 cut(s)	1193
SapI	GCTCTTC	1 cut(s)	668
SaqAI	TTAA	3 cut(s)	417, 425, 504
SatI	GCNGC	3 cut(s)	900, 1317, 1611
Sau3AI	GATC	3 cut(s)	286, 640, 811
Sau96I	GGNCC	4 cut(s)	18, 607, 752, 995
SchI	GAGTC	1 cut(s)	260
ScrFI	CCNGG	4 cut(s)	516, 696, 1561, 1581
SduI	GDGCHC	3 cut(s)	8, 532, 1193
SfaNI	GCATC	4 cut(s)	241, 715, 876, 1642
SfuI	TTCGAA	1 cut(s)	649
SgrAI	CRCCGGYG	1 cut(s)	217
SinI	GGWCC	3 cut(s)	18, 752, 995
SmiMI	CAYNNNNRTG	4 cut(s)	780, 1285, 1598, 1647
SmlI	CTYRAG	1 cut(s)	1427
SmoI	CTYRAG	1 cut(s)	1427
Sse9I	AATT	6 cut(s)	760, 858, 888, 1059, 1115, 1361
SsiI	CCGC	2 cut(s)	128, 155
SspI	AATATT	2 cut(s)	406, 563
SspMI	CTAG	2 cut(s)	747, 1053
SstI	GAGCTC	1 cut(s)	1193
StyD4I	CCNGG	4 cut(s)	514, 694, 1559, 1579
StyI	CCWWGG	3 cut(s)	548, 610, 1599
TaaI	ACNGT	4 cut(s)	1311, 1331, 1396, 1516
TaiI	ACGT	1 cut(s)	1153
TaqI	TCGA	3 cut(s)	649, 814, 1398
TasI	AATT	6 cut(s)	760, 858, 888, 1059, 1115, 1361
TatI	WGTACW	3 cut(s)	1055, 1289, 1629
TfiI	GAWTC	6 cut(s)	511, 539, 575, 1049, 1402, 1442
Tru1I	TTAA	3 cut(s)	417, 425, 504
Tru9I	TTAA	3 cut(s)	417, 425, 504
TscAI	CASTG	5 cut(s)	261, 771, 1134, 1223, 1336
TseI	GCWGC	3 cut(s)	899, 1316, 1610
TspGWI	ACGGA	2 cut(s)	536, 862
TspRI	CASTG	5 cut(s)	261, 771, 1134, 1223, 1336
VpaK11BI	GGWCC	3 cut(s)	18, 752, 995
XagI	CCTNNNNNAGG	1 cut(s)	1584
XapI	RAATTY	2 cut(s)	760, 1361
XceI	RCATGY	2 cut(s)	503, 1502
XspI	CTAG	2 cut(s)	747, 1053
Zsp2I	ATGCAT	1 cut(s)	1657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.