Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003242

Physical Location & Seq

Genomic Coordinates Reverse (-)

28881533 .. 28884060

Gene Structure 2528 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1192400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGAGTTTAATATCGCAGAATGTTCCGTCGGCTTTTAAAGCCAGCAAGACTAAAAGGGATGATGATGGTGTTACTGACGAGCCGCCTTTGCTTCTGCAAGGAAACGAAATCAATGGGGATGAAGAAGTGACTGATTTGGATATTGTGAGGACGAAGAAAAGAAAGAAAGCCCGTAAGGAAGATGATGAGAAAGTGGCAGTGGAGCAAAAAGAAAAGGAGCAGCTGGAGGACTCATTGTTTGGAGCCATTAATTCCCCACTTGAGTTTGGAAATGGGGATGAGGAAGAAGTTGGAGATGGAGCGGATTTGTTCTTTGTGGATCGTTCTGCCAGTGGTGTGCTTTCAGTTGATGAAGAGGATGGAGAGTTATCGGAAGAGGAAACTCAGGAAAGAAAACCTGTGTGGGTGGATGAGGAAGAGGAGAAGGCTAAAATAAATATTGCTAAAGTTAATAGGTTAAGGAAGCTGAGGAAGGAGGAGGAGGAGAGTTTGATTTCGGGTTCAGATTACGTGTCGAGATTGAGGGCTCAGCATGCTAAGCTGAACCCAGGCACAGAATGGGCTCAACTTGATCGGCACTTGCAGGAGAGTAGTTATTCTGATGATGAATCGTCGGATGAAGAGAATCGGGTAGGAGTGGCCCGTGGCTACAAGGGTGTAGAATCTGTTGATGATCTCCTTCGAACGAATGAGGAACTTGTTGTGAAGAGCAGTTCAAAGTTGTTGCCTGGACTTCTTGAATATTCAGCACTTCTAAATGCAAATGCAGAGGAACCTTCTAATGGTCCGATAAACTCAGTGCAGTTCCATAGAAACGGGCAGTTGCTTCTTGCTGCTGGGTTGGATCGAAGGCTTAGGTTTTTTCAAATTGATGGCAAGCGGAATACTAAAATAGAAAGCATCTTCCTTGATGACTGTCCCATTCGAAAGGCGTCTTTTTTGCCTGATGGGTCTCAGGTTATTATAGCTGGAAGGAGGAACTTCTTTTACAGCTTTGACTTAGTGAAGGCGAAAGTAGATAAAATTGGACCCCTGGTTGGTAGAGAGGAGAAAAGCTTGGAATTTTTTGAAGTTTCCCCTGACTCTAGTACAATTGCTTTTGTGGGCAATGAAGGCTATATCCTGTTGGTTTCCTCTAAAACGAAGGAATTGATCCGAACACTAAAGATGAATGGAACTGTTCGTTCTTTAGCTTTTACCAATGATGGGAAGCAACTGTTGAGCTCTGGCGGAGATGGACAGGTCTACCACTGGGATCTGAGAACTGGGGCTTGCATCCATAAGGCTGTTGATGAAGGTTGCATAAATGGAACATCTCTTTGTACATCTCCAACTGGAACATTGTTTGCTGCTGGTTCAGACAGCGGGATTGTCAACATTTACAATAGAGAGGATTTTTTGGGGGGCAAGAGAAAACCCATCAAGACCATCGAGAATCTTACAACGAAAGTGGACTTTCTCAAGTTCAACGGTGATGCTCAAATACTGGCCATTTGTTCTAGCATGAAGAAGGATAGCTTGAAGTTGATACATGTTCCATCATTTACTGTTTTCTCCAACTGGCCTCCACCGAGAAGGTCCATACATTATCCCCGCTGTTTGGATTTCAGTCCCGGTGGAGGTTTCATGGCTGTAGGAAATGCTGCTGGAAAAGTGTTGTTGTACAAGTTGCATCACTACCATCACGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

564

Amino Acids

62.51

Weight (kDa)

5.33

Isoelectric Point (pI)

46.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	260 - 428	1.5e-07	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	261 - 500	9e-18	Prp19 WD40 domain
WDR55	PF24796	263 - 466	2.4e-09	WDR55
Beta-prop_EML	PF23409	263 - 453	9.3e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st	PF24817	265 - 428	7.3e-11	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	310 - 558	1.6e-11	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	327 - 516	1.7e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	348 - 427	1.9e-06	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	351 - 429	4.4e-08	WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	351 - 452	2.2e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	356 - 521	1.6e-17	THOC3 beta-propeller domain
WDR55	PF24796	357 - 500	1.8e-07	WDR55
Beta-prop_EML_2	PF23414	358 - 498	2.1e-10	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14050
fragaria_vesca	FvH4_2g22480 FvH4_2g22480 FvH4_5g20520
malus_domestica	MD05G1205300.v1.1 MD05G1205400.v1.1 MD10G1192400.v1.1
prunus_persica	Prupe.5G054300_v2.0.a1 Prupe.5G054300_v2.0.a1 Prupe.8G231800_v2.0.a1
pyrus_communis	pycom05g19150 pycom12426g00550 pycom13g23680
rosa_chinensis	RchiOBHm_Chr6g0289431 RchiOBHm_Chr7g0205871 RchiOBHm_Chr7g0205881
rosa_laevigata	RLG00000012303
rosa_multiflora	Rmu_sc0000076.1_g000034 Rmu_sc0001527.1_g000035 Rmu_sc0004084.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251900 Rroxscaffold_7G00179220
rosa_rugosa	Rorug06G0203300 Rorug06G0203400 Rorug07G0093600
rosa_samantha	Rh6AG314600 Rh6BG322100 Rh6CG328300 Rh6DG314000 Rh7AG225300 Rh7BG221200 Rh7CG238900 Rh7CG239300 Rh7CG239400 Rh7DG232000
rosa_wichuraiana	Rw6G027180 Rw7G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	302
AccI	GTMKAC	1 cut(s)	1245
AciI	CCGC	6 cut(s)	83, 302, 880, 1230, 1365, 1594
AclWI	GGATC	4 cut(s)	327, 852, 1147, 1263
AcoI	YGGCCR	1 cut(s)	1488
AcsI	RAATTY	1 cut(s)	1061
AcyI	GRCGYC	1 cut(s)	932
AfaI	GTAC	3 cut(s)	1090, 1324, 1664
AfiI	CCNNNNNNNGG	5 cut(s)	782, 1037, 1206, 1600, 1619
AflIII	ACRYGT	2 cut(s)	510, 1531
AgsI	TTSAA	6 cut(s)	717, 740, 866, 1070, 1468, 1522
AjnI	CCWGG	3 cut(s)	547, 727, 1032
AluBI	AGCT	9 cut(s)	223, 466, 541, 968, 993, 1056, 1193, 1224, 1518
AluI	AGCT	9 cut(s)	223, 466, 541, 968, 993, 1056, 1193, 1224, 1518
Alw21I	GWGCWC	1 cut(s)	1226
Alw26I	GTCTC	1 cut(s)	957
AlwI	GGATC	4 cut(s)	327, 852, 1147, 1263
AoxI	GGCC	3 cut(s)	639, 1488, 1562
ApeKI	GCWGC	4 cut(s)	220, 833, 1349, 1643
ApoI	RAATTY	1 cut(s)	1061
ArsI	GACNNNNNNTTYG	2 cut(s)	221, 253
AseI	ATTAAT	1 cut(s)	249
AspS9I	GGNCC	4 cut(s)	640, 785, 1028, 1578
AsuC2I	CCSGG	1 cut(s)	1614
AsuHPI	GGTGA	1 cut(s)	1484
AsuII	TTCGAA	2 cut(s)	682, 925
AvaII	GGWCC	3 cut(s)	785, 1028, 1578
BalI	TGGCCA	1 cut(s)	1490
BanII	GRGCYC	3 cut(s)	529, 565, 1226
Bbv12I	GWGCWC	1 cut(s)	1226
BbvCI	CCTCAGC	1 cut(s)	467
BbvI	GCAGC	4 cut(s)	232, 820, 1336, 1630
BciT130I	CCWGG	3 cut(s)	549, 729, 1034
BcnI	CCSGG	1 cut(s)	1614
BcoDI	GTCTC	1 cut(s)	957
BfaI	CTAG	2 cut(s)	1086, 1500
BfmI	CTRYAG	1 cut(s)	1632
BisI	GCNGC	5 cut(s)	83, 221, 834, 1350, 1644
BlpI	GCTNAGC	2 cut(s)	528, 537
BlsI	GCNGC	5 cut(s)	84, 222, 835, 1351, 1645
Bme1390I	CCNGG	4 cut(s)	549, 729, 1034, 1614
Bme18I	GGWCC	3 cut(s)	785, 1028, 1578
BmgT120I	GGNCC	4 cut(s)	640, 785, 1028, 1578
BmiI	GGNNCC	3 cut(s)	244, 774, 1030
BmrFI	CCNGG	4 cut(s)	549, 729, 1034, 1614
BmrI	ACTGGG	2 cut(s)	1261, 1275
BmsI	GCATC	4 cut(s)	909, 1284, 1465, 1681
BmuI	ACTGGG	2 cut(s)	1261, 1275
BpmI	CTGGAG	1 cut(s)	245
Bpu10I	CCTNAGC	2 cut(s)	467, 854
Bpu1102I	GCTNAGC	2 cut(s)	528, 537
Bpu14I	TTCGAA	2 cut(s)	682, 925
BpuEI	CTTGAG	2 cut(s)	281, 1445
BpuMI	CCSGG	1 cut(s)	1614
BsaAI	YACGTR	1 cut(s)	511
BsaHI	GRCGYC	1 cut(s)	932
BsaI	GGTCTC	1 cut(s)	957
BsaJI	CCNNGG	3 cut(s)	547, 643, 1032
Bsc4I	CCNNNNNNNGG	5 cut(s)	782, 1037, 1206, 1600, 1619
Bse1I	ACTGG	6 cut(s)	330, 1256, 1270, 1339, 1491, 1565
Bse3DI	GCAATG	1 cut(s)	1114
BseBI	CCWGG	3 cut(s)	549, 729, 1034
BseDI	CCNNGG	3 cut(s)	547, 643, 1032
BseGI	GGATG	7 cut(s)	64, 124, 283, 364, 415, 622, 1275
BseLI	CCNNNNNNNGG	5 cut(s)	782, 1037, 1206, 1600, 1619
BseMI	GCAATG	1 cut(s)	1114
BseMII	CTCAG	6 cut(s)	398, 458, 542, 810, 968, 1250
BseNI	ACTGG	6 cut(s)	330, 1256, 1270, 1339, 1491, 1565
BseRI	GAGGAG	5 cut(s)	434, 491, 494, 497, 1061
BseXI	GCAGC	4 cut(s)	232, 820, 1336, 1630
BseYI	CCCAGC	1 cut(s)	836
BsgI	GTGCAG	1 cut(s)	821
BshFI	GGCC	3 cut(s)	641, 1490, 1564
BsiHKAI	GWGCWC	1 cut(s)	1226
BsiSI	CCGG	1 cut(s)	1614
BslFI	GGGAC	2 cut(s)	903, 1596
BslI	CCNNNNNNNGG	5 cut(s)	782, 1037, 1206, 1600, 1619
BsmAI	GTCTC	1 cut(s)	957
BsmFI	GGGAC	2 cut(s)	903, 1596
BsnI	GGCC	3 cut(s)	641, 1490, 1564
Bso31I	GGTCTC	1 cut(s)	957
Bsp119I	TTCGAA	2 cut(s)	682, 925
Bsp1286I	GDGCHC	3 cut(s)	529, 565, 1226
Bsp1407I	TGTACA	2 cut(s)	1322, 1662
Bsp143I	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
Bsp1720I	GCTNAGC	2 cut(s)	528, 537
BspACI	CCGC	6 cut(s)	83, 302, 880, 1230, 1365, 1594
BspANI	GGCC	3 cut(s)	641, 1490, 1564
BspCNI	CTCAG	6 cut(s)	397, 459, 541, 809, 967, 1251
BspLI	GGNNCC	3 cut(s)	244, 774, 1030
BspPI	GGATC	4 cut(s)	327, 852, 1147, 1263
BspQI	GCTCTTC	1 cut(s)	701
BspT104I	TTCGAA	2 cut(s)	682, 925
BspTNI	GGTCTC	1 cut(s)	957
BsrBI	CCGCTC	1 cut(s)	302
BsrDI	GCAATG	1 cut(s)	1114
BsrGI	TGTACA	2 cut(s)	1322, 1662
BsrI	ACTGG	6 cut(s)	330, 1256, 1270, 1339, 1491, 1565
BssECI	CCNNGG	3 cut(s)	547, 643, 1032
BssMI	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
BssNI	GRCGYC	1 cut(s)	932
Bst2UI	CCWGG	3 cut(s)	549, 729, 1034
Bst4CI	ACNGT	5 cut(s)	917, 1180, 1218, 1472, 1549
Bst6I	CTCTTC	5 cut(s)	348, 369, 411, 615, 701
BstACI	GRCGYC	1 cut(s)	932
BstAUI	TGTACA	2 cut(s)	1322, 1662
BstBAI	YACGTR	1 cut(s)	511
BstBI	TTCGAA	2 cut(s)	682, 925
BstC8I	GCNNGC	4 cut(s)	43, 534, 878, 1273
BstDEI	CTNAG	9 cut(s)	384, 467, 528, 537, 796, 854, 954, 1000, 1259
BstDSI	CCRYGG	1 cut(s)	643
BstF5I	GGATG	7 cut(s)	64, 124, 283, 364, 415, 622, 1275
BstKTI	GATC	6 cut(s)	322, 574, 676, 847, 1155, 1258
BstMAI	GTCTC	1 cut(s)	957
BstMBI	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
BstMWI	GCNNNNNNNGC	5 cut(s)	38, 88, 533, 538, 1113
BstNI	CCWGG	3 cut(s)	549, 729, 1034
BstNSI	RCATGY	2 cut(s)	536, 1535
BstSCI	CCNGG	4 cut(s)	547, 727, 1032, 1612
BstSFI	CTRYAG	1 cut(s)	1632
BstV1I	GCAGC	4 cut(s)	232, 820, 1336, 1630
BstX2I	RGATCY	1 cut(s)	1255
BstYI	RGATCY	1 cut(s)	1255
BsuRI	GGCC	3 cut(s)	641, 1490, 1564
BtgI	CCRYGG	1 cut(s)	643
BtsCI	GGATG	7 cut(s)	64, 124, 283, 364, 415, 622, 1275
BtsI	GCAGTG	1 cut(s)	204
BtsIMutI	CAGTG	4 cut(s)	204, 337, 804, 1249
Cac8I	GCNNGC	4 cut(s)	43, 534, 878, 1273
Cfr13I	GGNCC	4 cut(s)	640, 785, 1028, 1578
CseI	GACGC	1 cut(s)	921
Csp6I	GTAC	3 cut(s)	1089, 1323, 1663
CspCI	CAANNNNNGTGG	2 cut(s)	1431, 1466
CviAII	CATG	4 cut(s)	533, 1504, 1532, 1627
CviQI	GTAC	3 cut(s)	1089, 1323, 1663
DdeI	CTNAG	9 cut(s)	384, 467, 528, 537, 796, 854, 954, 1000, 1259
DpnI	GATC	6 cut(s)	321, 573, 675, 846, 1154, 1257
DpnII	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
DraI	TTTAAA	1 cut(s)	37
EaeI	YGGCCR	1 cut(s)	1488
Eam1104I	CTCTTC	5 cut(s)	348, 369, 411, 615, 701
EarI	CTCTTC	5 cut(s)	348, 369, 411, 615, 701
EciI	GGCGGA	1 cut(s)	1245
Ecl136II	GAGCTC	1 cut(s)	1224
Eco24I	GRGCYC	3 cut(s)	529, 565, 1226
Eco31I	GGTCTC	1 cut(s)	957
Eco47I	GGWCC	3 cut(s)	785, 1028, 1578
Eco53kI	GAGCTC	1 cut(s)	1224
EcoICRI	GAGCTC	1 cut(s)	1224
EcoRII	CCWGG	3 cut(s)	547, 727, 1032
EcoT38I	GRGCYC	3 cut(s)	529, 565, 1226
FaeI	CATG	4 cut(s)	536, 1507, 1535, 1630
FalI	AAGNNNNNCTT	2 cut(s)	1502, 1534
FaqI	GGGAC	2 cut(s)	903, 1596
FatI	CATG	4 cut(s)	532, 1503, 1531, 1626
FauI	CCCGC	2 cut(s)	1358, 1601
FblI	GTMKAC	1 cut(s)	1245
Fnu4HI	GCNGC	5 cut(s)	83, 221, 834, 1350, 1644
FokI	GGATG	7 cut(s)	71, 131, 290, 371, 422, 629, 1262
FriOI	GRGCYC	3 cut(s)	529, 565, 1226
Fsp4HI	GCNGC	5 cut(s)	83, 221, 834, 1350, 1644
FspBI	CTAG	2 cut(s)	1086, 1500
GluI	GCNGC	5 cut(s)	83, 221, 834, 1350, 1644
GsaI	CCCAGC	1 cut(s)	840
GsuI	CTGGAG	1 cut(s)	245
HaeIII	GGCC	3 cut(s)	641, 1490, 1564
HapII	CCGG	1 cut(s)	1614
HgaI	GACGC	1 cut(s)	921
Hin1I	GRCGYC	1 cut(s)	932
Hin1II	CATG	4 cut(s)	536, 1507, 1535, 1630
HincII	GTYRAC	1 cut(s)	1375
HindII	GTYRAC	1 cut(s)	1375
HindIII	AAGCTT	1 cut(s)	1054
HinfI	GANTC	6 cut(s)	230, 608, 625, 662, 1082, 1435
HpaII	CCGG	1 cut(s)	1614
HphI	GGTGA	1 cut(s)	1484
Hpy166II	GTNNAC	3 cut(s)	1246, 1375, 1453
Hpy188I	TCNGA	8 cut(s)	373, 505, 601, 616, 789, 1157, 1260, 1360
Hpy188III	TCNNGA	5 cut(s)	386, 516, 737, 1423, 1432
Hpy8I	GTNNAC	3 cut(s)	1246, 1375, 1453
Hpy99I	CGWCG	2 cut(s)	31, 616
HpyCH4III	ACNGT	5 cut(s)	917, 1180, 1218, 1472, 1549
HpyCH4IV	ACGT	1 cut(s)	510
HpyCH4V	TGCA	8 cut(s)	97, 583, 761, 767, 802, 1275, 1302, 1672
HpyF10VI	GCNNNNNNNGC	5 cut(s)	38, 88, 533, 538, 1113
HpyF3I	CTNAG	9 cut(s)	384, 467, 528, 537, 796, 854, 954, 1000, 1259
HpySE526I	ACGT	1 cut(s)	510
Hsp92I	GRCGYC	1 cut(s)	932
Hsp92II	CATG	4 cut(s)	536, 1507, 1535, 1630
Kzo9I	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
LguI	GCTCTTC	1 cut(s)	701
LmnI	GCTCC	4 cut(s)	202, 217, 242, 299
Lsp1109I	GCAGC	4 cut(s)	232, 820, 1336, 1630
LweI	GCATC	4 cut(s)	909, 1284, 1465, 1681
MaeI	CTAG	2 cut(s)	1086, 1500
MaeII	ACGT	1 cut(s)	510
MaeIII	GTNAC	2 cut(s)	70, 127
MalI	GATC	6 cut(s)	321, 573, 675, 846, 1154, 1257
MbiI	CCGCTC	1 cut(s)	302
MboI	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
MfeI	CAATTG	1 cut(s)	1092
MflI	RGATCY	1 cut(s)	1255
MhlI	GDGCHC	3 cut(s)	529, 565, 1226
MlsI	TGGCCA	1 cut(s)	1490
MluCI	AATT	6 cut(s)	250, 867, 1023, 1061, 1092, 1148
MluNI	TGGCCA	1 cut(s)	1490
MlyI	GAGTC	2 cut(s)	224, 1076
MmeI	TCCRAC	5 cut(s)	271, 594, 822, 1355, 1581
Mox20I	TGGCCA	1 cut(s)	1490
MscI	TGGCCA	1 cut(s)	1490
MseI	TTAA	5 cut(s)	8, 36, 249, 450, 458
Msp20I	TGGCCA	1 cut(s)	1490
MspA1I	CMGCKG	3 cut(s)	223, 1365, 1596
MspI	CCGG	1 cut(s)	1614
MspR9I	CCNGG	4 cut(s)	549, 729, 1034, 1614
MunI	CAATTG	1 cut(s)	1092
MvaI	CCWGG	3 cut(s)	549, 729, 1034
MwoI	GCNNNNNNNGC	5 cut(s)	38, 88, 533, 538, 1113
NciI	CCSGG	1 cut(s)	1614
NdeII	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
NlaIII	CATG	4 cut(s)	536, 1507, 1535, 1630
NlaIV	GGNNCC	3 cut(s)	244, 774, 1030
NmuCI	GTSAC	1 cut(s)	127
NspI	RCATGY	2 cut(s)	536, 1535
NspV	TTCGAA	2 cut(s)	682, 925
PaeI	GCATGC	1 cut(s)	536
PciI	ACATGT	1 cut(s)	1531
PciSI	GCTCTTC	1 cut(s)	701
PfeI	GAWTC	4 cut(s)	608, 625, 662, 1435
PkrI	GCNGC	5 cut(s)	84, 222, 835, 1351, 1645
PleI	GAGTC	2 cut(s)	224, 1076
PpsI	GAGTC	2 cut(s)	224, 1076
Ppu21I	YACGTR	1 cut(s)	511
PscI	ACATGT	1 cut(s)	1531
PshBI	ATTAAT	1 cut(s)	249
Psp124BI	GAGCTC	1 cut(s)	1226
Psp6I	CCWGG	3 cut(s)	547, 727, 1032
PspFI	CCCAGC	1 cut(s)	836
PspGI	CCWGG	3 cut(s)	547, 727, 1032
PspN4I	GGNNCC	3 cut(s)	244, 774, 1030
PspPI	GGNCC	4 cut(s)	640, 785, 1028, 1578
PsrI	GAACNNNNNNTAC	2 cut(s)	971, 1003
PsuI	RGATCY	1 cut(s)	1255
PvuII	CAGCTG	1 cut(s)	223
RsaI	GTAC	3 cut(s)	1090, 1324, 1664
RsaNI	GTAC	3 cut(s)	1089, 1323, 1663
SacI	GAGCTC	1 cut(s)	1226
SapI	GCTCTTC	1 cut(s)	701
SaqAI	TTAA	5 cut(s)	8, 36, 249, 450, 458
SatI	GCNGC	5 cut(s)	83, 221, 834, 1350, 1644
Sau3AI	GATC	6 cut(s)	319, 571, 673, 844, 1152, 1255
Sau96I	GGNCC	4 cut(s)	640, 785, 1028, 1578
SchI	GAGTC	2 cut(s)	224, 1076
ScrFI	CCNGG	4 cut(s)	549, 729, 1034, 1614
SduI	GDGCHC	3 cut(s)	529, 565, 1226
SfaNI	GCATC	4 cut(s)	909, 1284, 1465, 1681
SfcI	CTRYAG	1 cut(s)	1632
SfuI	TTCGAA	2 cut(s)	682, 925
SinI	GGWCC	3 cut(s)	785, 1028, 1578
SmlI	CTYRAG	2 cut(s)	260, 1460
SmoI	CTYRAG	2 cut(s)	260, 1460
SphI	GCATGC	1 cut(s)	536
Sse9I	AATT	6 cut(s)	250, 867, 1023, 1061, 1092, 1148
SsiI	CCGC	6 cut(s)	83, 302, 880, 1230, 1365, 1594
SspI	AATATT	2 cut(s)	439, 743
SspMI	CTAG	2 cut(s)	1086, 1500
SstI	GAGCTC	1 cut(s)	1226
StyD4I	CCNGG	4 cut(s)	547, 727, 1032, 1612
TaaI	ACNGT	5 cut(s)	917, 1180, 1218, 1472, 1549
TaiI	ACGT	1 cut(s)	513
TaqI	TCGA	5 cut(s)	515, 682, 847, 925, 1431
TasI	AATT	6 cut(s)	250, 867, 1023, 1061, 1092, 1148
TatI	WGTACW	3 cut(s)	1088, 1322, 1662
TauI	GCSGC	1 cut(s)	85
TfiI	GAWTC	4 cut(s)	608, 625, 662, 1435
Tru1I	TTAA	5 cut(s)	8, 36, 249, 450, 458
Tru9I	TTAA	5 cut(s)	8, 36, 249, 450, 458
TscAI	CASTG	4 cut(s)	204, 337, 804, 1256
TseFI	GTSAC	1 cut(s)	127
TseI	GCWGC	4 cut(s)	220, 833, 1349, 1643
Tsp45I	GTSAC	1 cut(s)	127
TspDTI	ATGAA	9 cut(s)	135, 366, 621, 633, 1125, 1184, 1308, 1520, 1615
TspGWI	ACGGA	1 cut(s)	15
TspRI	CASTG	4 cut(s)	204, 337, 804, 1256
VpaK11BI	GGWCC	3 cut(s)	785, 1028, 1578
VspI	ATTAAT	1 cut(s)	249
XapI	RAATTY	1 cut(s)	1061
XceI	RCATGY	2 cut(s)	536, 1535
XcmI	CCANNNNNNNNNTGG	1 cut(s)	555
XmiI	GTMKAC	1 cut(s)	1245
XspI	CTAG	2 cut(s)	1086, 1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.