Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0003242

Physical Location & Seq

Genomic Coordinates Forward (+)

55265544 .. 55279117

Gene Structure 13574 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG322100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGAGTTTGATATCGCAGAATATCCCCTCGGCTTTTAAATCCAACAAGAGCAAAAGGGAGGTTCCCGATGAGCCTGCGTTTATGCAAGCGAATGATGAGAATGGAGAGGGGAAAGAAATGAGTTTGGATGTTATGAGGGTGAACAAGAGAAAGAAACAGCGCAATGAAGTTGATGAGAAAGACGCGGTGGAGCAAGAGAAGGAAATGAAGAAGCTGGAGAGCTTTTTGTTTGGAAGCATTCATGCGCCAGTGGAGTTTGGGAAGGAGGATGAGGATGAAGCGGGAGATGCAGTGGATAACGGCTCTTCTTTGTTCTTTGTGGATCGTTCTGCAAATAATGTGCTTTCTGTTGATGAAGATGATGGAGAAGTATTGGAAGAGGAAACTAAGCAAAGGAAAGCTGTCTGGGAGGATGATGAAGAGGCAAAGGCCAATGTGAATATTGCCAAAGTTAACAGATTAAGGAAGCTGAGGAAGGAAGAGGATGAGAGTTTGATTTCTGGTTCTGAGTATGTTTCAAGATTGAGGGCTCAACATGTTAAGCTGAACCCGGGAACGGAGTGGGCTCAACTTGATTCAGAGCCAAGGAACAATAGATATTCTGACGATGAATCATCAGATGATGAAGATAGGGCAGTAGTGGCCCGTGGCTACAAGGATGTTGGAGCTGTTGATGATCTCCTTCGAACAAATGAGGATCTTGTTGTGAAGAGCAGTTCAAAACTGTTGCCTGGGCTTCTTGAATATTCAATACTGACAGATGCTAACGCAGAGGAACCTTCTAATGGTCCTATAAATTCAGTGCAGTTTCATAGAAATGCTCAGTTGCTTCTGACTGCTGGATTGGATCGAAGGCTTAGGTTTTTCCAAATTGATGGAAAAAGGAATGCTAAAATCGAAAGCATATTCATTGATGACTGTCCAATTCGGAAAGCAGCCTTCTTGCCTGATGGCTCTCAGGTTATTATAGCTGGAAGGAGGAAGTTCTTTTACAGCTTTGATTTAGTGAAGGCCAAAGTTGATAGAATAGGACCCCTCGTTGGTAGAGAGGAAAAAAGCTTGGAAGTTTTTGAGGTTTCCCCTGATTCTAGTACAATTGCCTTTGTGGGCAATGAAGGATATATCTTGTTGGTTTCCTCTAAAACAAAGGAATTGATTGGGACACTGAAGATGAATGGAACTGCTCGTTCTTTAGCTTTTTCTGAAGATGGGAAGCAATTATTGAGCTCTGGCGGAGATGGACAGATTTACCACTGGGATTTGAGAACAGGAGCTTGCTTCCATAAGGCTGTTGATGAAGGTTGTATAAATGGCACTGCTCTGTGTACATCTCCAAATGGAAAACTGTTTGCTGCTGGTTCAGACAGTGGGATTGTCAATATCTACAACAGAGAGGAGTTCTTAGGGGGCAAGAGAAAACCCATCAAGACAGTCGAAAATCTCACAACCAAAGTAGATTTTCTCAAGTTCAATACTGATTCTCAAATACTGGCTATTTGTTCGAGCATGAAGAAGAACAGCTTGAAGTTGGTGCATGTTCCATCATTGACTGTGTTCTCTAACTGGCCTCCGCAAAAGAAGACCCTGCACTATCCCCGGTGTTTGGACTTCAGTCCTGGTGGAGGTTTCATGGCCATGGGAAATGCTGCTGGAAAAGTGTTGCTGTACAAGTTGCATCACTACCATGATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0030686 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

564

Amino Acids

62.79

Weight (kDa)

5.49

Isoelectric Point (pI)

43.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	261 - 429	4.1e-06	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	262 - 501	7e-19	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	266 - 429	6.3e-09	WDHD1 first WD40 domain
WD40_Gbeta	PF25391	268 - 462	1.4e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	269 - 425	1.3e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	311 - 559	1.5e-11	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	334 - 518	1.6e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	347 - 428	6.8e-07	WDR36/Utp21 first beta-propeller
WDR55	PF24796	357 - 527	1.7e-08	WDR55
Beta-prop_THOC3	PF25174	358 - 545	1.3e-18	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	359 - 502	1.1e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML_2	PF23414	440 - 559	1.8e-06	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14050
fragaria_vesca	FvH4_2g22480 FvH4_2g22480 FvH4_5g20520
malus_domestica	MD05G1205300.v1.1 MD05G1205400.v1.1 MD10G1192400.v1.1
prunus_persica	Prupe.5G054300_v2.0.a1 Prupe.5G054300_v2.0.a1 Prupe.8G231800_v2.0.a1
pyrus_communis	pycom05g19150 pycom12426g00550 pycom13g23680
rosa_chinensis	RchiOBHm_Chr6g0289431 RchiOBHm_Chr7g0205871 RchiOBHm_Chr7g0205881
rosa_laevigata	RLG00000012303
rosa_multiflora	Rmu_sc0000076.1_g000034 Rmu_sc0001527.1_g000035 Rmu_sc0004084.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251900 Rroxscaffold_7G00179220
rosa_rugosa	Rorug06G0203300 Rorug06G0203400 Rorug07G0093600
rosa_samantha	Rh6AG314600 Rh6BG322100 Rh6CG328300 Rh6DG314000 Rh7AG225300 Rh7BG221200 Rh7CG238900 Rh7CG239300 Rh7CG239400 Rh7DG232000
rosa_wichuraiana	Rw6G027180 Rw7G019430

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	185
AciI	CCGC	4 cut(s)	185, 281, 1233, 1571
AclWI	GGATC	3 cut(s)	330, 705, 855
AcoI	YGGCCR	1 cut(s)	1632
AcsI	RAATTY	1 cut(s)	796
AcuI	CTGAAG	3 cut(s)	1187, 1224, 1594
AfaI	GTAC	3 cut(s)	1093, 1327, 1667
AfiI	CCNNNNNNNGG	5 cut(s)	556, 785, 1040, 1603, 1622
AflIII	ACRYGT	1 cut(s)	535
AgsI	TTSAA	6 cut(s)	519, 720, 743, 750, 1471, 1525
AjnI	CCWGG	2 cut(s)	730, 1615
Alw21I	GWGCWC	1 cut(s)	1229
AlwI	GGATC	3 cut(s)	330, 705, 855
Ama87I	CYCGRG	1 cut(s)	550
AoxI	GGCC	5 cut(s)	429, 642, 1011, 1565, 1632
ApeKI	GCWGC	3 cut(s)	935, 1352, 1646
ApoI	RAATTY	1 cut(s)	796
AspLEI	GCGC	2 cut(s)	162, 247
AspS9I	GGNCC	3 cut(s)	643, 788, 1031
AsuC2I	CCSGG	3 cut(s)	551, 552, 1597
AsuHPI	GGTGA	1 cut(s)	151
AsuII	TTCGAA	1 cut(s)	685
AvaI	CYCGRG	1 cut(s)	550
AvaII	GGWCC	2 cut(s)	788, 1031
BalI	TGGCCA	1 cut(s)	1634
BanII	GRGCYC	3 cut(s)	532, 568, 1229
BarI	GAAGNNNNNNTAC	2 cut(s)	974, 1006
BbsI	GAAGAC	1 cut(s)	1586
Bbv12I	GWGCWC	1 cut(s)	1229
BbvCI	CCTCAGC	1 cut(s)	470
BbvI	GCAGC	3 cut(s)	947, 1339, 1633
BccI	CCATC	7 cut(s)	356, 869, 944, 1202, 1232, 1430, 1549
BceAI	ACGGC	1 cut(s)	316
BcgI	CGANNNNNNTGC	2 cut(s)	56, 90
BciT130I	CCWGG	2 cut(s)	732, 1617
BcnI	CCSGG	3 cut(s)	551, 552, 1597
BfaI	CTAG	1 cut(s)	1089
BisI	GCNGC	3 cut(s)	936, 1353, 1647
BlsI	GCNGC	3 cut(s)	937, 1354, 1648
Bme1390I	CCNGG	5 cut(s)	551, 552, 732, 1597, 1617
Bme18I	GGWCC	2 cut(s)	788, 1031
BmeT110I	CYCGRG	1 cut(s)	550
BmgT120I	GGNCC	3 cut(s)	643, 788, 1031
BmiI	GGNNCC	3 cut(s)	63, 777, 1033
BmrFI	CCNGG	5 cut(s)	551, 552, 732, 1597, 1617
BmrI	ACTGGG	1 cut(s)	1264
BmsI	GCATC	4 cut(s)	277, 751, 1678, 1684
BmuI	ACTGGG	1 cut(s)	1264
BoxI	GACNNNNGTC	1 cut(s)	1611
BpiI	GAAGAC	1 cut(s)	1586
BpmI	CTGGAG	1 cut(s)	236
Bpu10I	CCTNAGC	2 cut(s)	470, 857
Bpu14I	TTCGAA	1 cut(s)	685
BpuEI	CTTGAG	1 cut(s)	1448
BpuMI	CCSGG	3 cut(s)	551, 552, 1597
BsaJI	CCNNGG	7 cut(s)	27, 550, 584, 646, 731, 1595, 1635
BsaXI	ACNNNNNCTCC	2 cut(s)	276, 306
Bsc4I	CCNNNNNNNGG	5 cut(s)	556, 785, 1040, 1603, 1622
Bse1I	ACTGG	4 cut(s)	248, 1259, 1494, 1568
Bse3DI	GCAATG	2 cut(s)	169, 1117
BseBI	CCWGG	2 cut(s)	732, 1617
BseDI	CCNNGG	7 cut(s)	27, 550, 584, 646, 731, 1595, 1635
BseGI	GGATG	6 cut(s)	133, 274, 280, 418, 490, 664
BseLI	CCNNNNNNNGG	5 cut(s)	556, 785, 1040, 1603, 1622
BseMI	GCAATG	2 cut(s)	169, 1117
BseMII	CTCAG	4 cut(s)	461, 498, 836, 971
BseNI	ACTGG	4 cut(s)	248, 1259, 1494, 1568
BseRI	GAGGAG	1 cut(s)	1409
BseXI	GCAGC	3 cut(s)	947, 1339, 1633
BsgI	GTGCAG	2 cut(s)	824, 1571
Bsh1236I	CGCG	1 cut(s)	185
BshFI	GGCC	5 cut(s)	431, 644, 1013, 1567, 1634
BsiHKAI	GWGCWC	1 cut(s)	1229
BsiHKCI	CYCGRG	1 cut(s)	550
BsiSI	CCGG	2 cut(s)	551, 1597
BslFI	GGGAC	1 cut(s)	1174
BslI	CCNNNNNNNGG	5 cut(s)	556, 785, 1040, 1603, 1622
BsmFI	GGGAC	1 cut(s)	1174
BsmI	GAATGC	2 cut(s)	237, 892
BsnI	GGCC	5 cut(s)	431, 644, 1013, 1567, 1634
BsoBI	CYCGRG	1 cut(s)	550
Bsp119I	TTCGAA	1 cut(s)	685
Bsp1286I	GDGCHC	3 cut(s)	532, 568, 1229
Bsp1407I	TGTACA	2 cut(s)	1325, 1665
Bsp143I	GATC	4 cut(s)	322, 676, 697, 847
Bsp19I	CCATGG	1 cut(s)	1635
BspACI	CCGC	4 cut(s)	185, 281, 1233, 1571
BspANI	GGCC	5 cut(s)	431, 644, 1013, 1567, 1634
BspCNI	CTCAG	4 cut(s)	462, 499, 835, 970
BspFNI	CGCG	1 cut(s)	185
BspLI	GGNNCC	3 cut(s)	63, 777, 1033
BspPI	GGATC	3 cut(s)	330, 705, 855
BspQI	GCTCTTC	2 cut(s)	310, 704
BspT104I	TTCGAA	1 cut(s)	685
BsrDI	GCAATG	2 cut(s)	169, 1117
BsrGI	TGTACA	2 cut(s)	1325, 1665
BsrI	ACTGG	4 cut(s)	248, 1259, 1494, 1568
BssECI	CCNNGG	7 cut(s)	27, 550, 584, 646, 731, 1595, 1635
BssMI	GATC	4 cut(s)	322, 676, 697, 847
BssT1I	CCWWGG	2 cut(s)	584, 1635
Bst2UI	CCWGG	2 cut(s)	732, 1617
Bst4CI	ACNGT	6 cut(s)	726, 920, 1347, 1367, 1432, 1552
Bst6I	CTCTTC	5 cut(s)	310, 372, 414, 474, 704
BstAUI	TGTACA	2 cut(s)	1325, 1665
BstBI	TTCGAA	1 cut(s)	685
BstC8I	GCNNGC	3 cut(s)	75, 87, 1276
BstDEI	CTNAG	7 cut(s)	387, 470, 507, 822, 857, 957, 1402
BstDSI	CCRYGG	2 cut(s)	646, 1635
BstENI	CCTNNNNNAGG	1 cut(s)	1620
BstF5I	GGATG	6 cut(s)	133, 274, 280, 418, 490, 664
BstFNI	CGCG	1 cut(s)	185
BstHHI	GCGC	2 cut(s)	162, 247
BstKTI	GATC	4 cut(s)	325, 679, 700, 850
BstMBI	GATC	4 cut(s)	322, 676, 697, 847
BstMWI	GCNNNNNNNGC	2 cut(s)	287, 641
BstNI	CCWGG	2 cut(s)	732, 1617
BstNSI	RCATGY	2 cut(s)	539, 1538
BstPAI	GACNNNNGTC	1 cut(s)	1611
BstSCI	CCNGG	5 cut(s)	549, 550, 730, 1595, 1615
BstUI	CGCG	1 cut(s)	185
BstV1I	GCAGC	3 cut(s)	947, 1339, 1633
BstV2I	GAAGAC	1 cut(s)	1586
BstX2I	RGATCY	1 cut(s)	697
BstXI	CCANNNNNNTGG	1 cut(s)	875
BstYI	RGATCY	1 cut(s)	697
BsuRI	GGCC	5 cut(s)	431, 644, 1013, 1567, 1634
BtgI	CCRYGG	2 cut(s)	646, 1635
BtsCI	GGATG	6 cut(s)	133, 274, 280, 418, 490, 664
BtsI	GCAGTG	2 cut(s)	297, 1314
BtsIMutI	CAGTG	7 cut(s)	255, 297, 807, 1163, 1252, 1314, 1372
Cac8I	GCNNGC	3 cut(s)	75, 87, 1276
CfoI	GCGC	2 cut(s)	162, 247
Cfr13I	GGNCC	3 cut(s)	643, 788, 1031
Cfr9I	CCCGGG	1 cut(s)	550
CseI	GACGC	1 cut(s)	191
Csp6I	GTAC	3 cut(s)	1092, 1326, 1666
CviAII	CATG	7 cut(s)	242, 536, 1507, 1535, 1630, 1636, 1685
CviQI	GTAC	3 cut(s)	1092, 1326, 1666
DdeI	CTNAG	7 cut(s)	387, 470, 507, 822, 857, 957, 1402
DpnI	GATC	4 cut(s)	324, 678, 699, 849
DpnII	GATC	4 cut(s)	322, 676, 697, 847
DraI	TTTAAA	1 cut(s)	37
EaeI	YGGCCR	1 cut(s)	1632
Eam1104I	CTCTTC	5 cut(s)	310, 372, 414, 474, 704
EarI	CTCTTC	5 cut(s)	310, 372, 414, 474, 704
EciI	GGCGGA	1 cut(s)	1248
Ecl136II	GAGCTC	1 cut(s)	1227
Eco130I	CCWWGG	2 cut(s)	584, 1635
Eco24I	GRGCYC	3 cut(s)	532, 568, 1229
Eco32I	GATATC	1 cut(s)	12
Eco47I	GGWCC	2 cut(s)	788, 1031
Eco53kI	GAGCTC	1 cut(s)	1227
Eco57I	CTGAAG	3 cut(s)	1187, 1224, 1594
Eco88I	CYCGRG	1 cut(s)	550
EcoICRI	GAGCTC	1 cut(s)	1227
EcoNI	CCTNNNNNAGG	1 cut(s)	1620
EcoO109I	RGGNCCY	1 cut(s)	1031
EcoRII	CCWGG	2 cut(s)	730, 1615
EcoRV	GATATC	1 cut(s)	12
EcoT14I	CCWWGG	2 cut(s)	584, 1635
EcoT22I	ATGCAT	1 cut(s)	1693
EcoT38I	GRGCYC	3 cut(s)	532, 568, 1229
ErhI	CCWWGG	2 cut(s)	584, 1635
FaeI	CATG	7 cut(s)	245, 539, 1510, 1538, 1633, 1639, 1688
FalI	AAGNNNNNCTT	2 cut(s)	1505, 1537
FaqI	GGGAC	1 cut(s)	1174
FatI	CATG	7 cut(s)	241, 535, 1506, 1534, 1629, 1635, 1684
FauI	CCCGC	1 cut(s)	274
Fnu4HI	GCNGC	3 cut(s)	936, 1353, 1647
FokI	GGATG	6 cut(s)	140, 281, 287, 425, 497, 671
FriOI	GRGCYC	3 cut(s)	532, 568, 1229
Fsp4HI	GCNGC	3 cut(s)	936, 1353, 1647
FspBI	CTAG	1 cut(s)	1089
GlaI	GCGC	2 cut(s)	161, 246
GluI	GCNGC	3 cut(s)	936, 1353, 1647
GsuI	CTGGAG	1 cut(s)	236
HaeIII	GGCC	5 cut(s)	431, 644, 1013, 1567, 1634
HapII	CCGG	2 cut(s)	551, 1597
HgaI	GACGC	1 cut(s)	191
HhaI	GCGC	2 cut(s)	162, 247
Hin1II	CATG	7 cut(s)	245, 539, 1510, 1538, 1633, 1639, 1688
Hin6I	GCGC	2 cut(s)	160, 245
HinP1I	GCGC	2 cut(s)	160, 245
HincII	GTYRAC	1 cut(s)	454
HindII	GTYRAC	1 cut(s)	454
HindIII	AAGCTT	1 cut(s)	1057
HinfI	GANTC	4 cut(s)	575, 611, 1085, 1478
HpaI	GTTAAC	1 cut(s)	454
HpaII	CCGG	2 cut(s)	551, 1597
HphI	GGTGA	1 cut(s)	151
Hpy166II	GTNNAC	3 cut(s)	142, 454, 1326
Hpy188I	TCNGA	8 cut(s)	508, 580, 604, 619, 834, 930, 1204, 1363
Hpy188III	TCNNGA	4 cut(s)	65, 519, 740, 1426
Hpy8I	GTNNAC	3 cut(s)	142, 454, 1326
HpyCH4III	ACNGT	6 cut(s)	726, 920, 1347, 1367, 1432, 1552
HpyCH4V	TGCA	8 cut(s)	85, 290, 332, 805, 1534, 1588, 1675, 1691
HpyF10VI	GCNNNNNNNGC	2 cut(s)	287, 641
HpyF3I	CTNAG	7 cut(s)	387, 470, 507, 822, 857, 957, 1402
Hsp92II	CATG	7 cut(s)	245, 539, 1510, 1538, 1633, 1639, 1688
HspAI	GCGC	2 cut(s)	160, 245
KspAI	GTTAAC	1 cut(s)	454
Kzo9I	GATC	4 cut(s)	322, 676, 697, 847
LguI	GCTCTTC	2 cut(s)	310, 704
LmnI	GCTCC	3 cut(s)	190, 665, 1271
Lsp1109I	GCAGC	3 cut(s)	947, 1339, 1633
LweI	GCATC	4 cut(s)	277, 751, 1678, 1684
MaeI	CTAG	1 cut(s)	1089
MalI	GATC	4 cut(s)	324, 678, 699, 849
MboI	GATC	4 cut(s)	322, 676, 697, 847
MfeI	CAATTG	1 cut(s)	1095
MflI	RGATCY	1 cut(s)	697
MhlI	GDGCHC	3 cut(s)	532, 568, 1229
MlsI	TGGCCA	1 cut(s)	1634
MluCI	AATT	6 cut(s)	796, 870, 924, 1095, 1151, 1217
MluNI	TGGCCA	1 cut(s)	1634
MmeI	TCCRAC	2 cut(s)	66, 643
Mox20I	TGGCCA	1 cut(s)	1634
Mph1103I	ATGCAT	1 cut(s)	1693
MscI	TGGCCA	1 cut(s)	1634
MseI	TTAA	4 cut(s)	36, 453, 461, 540
MslI	CAYNNNNRTG	3 cut(s)	816, 1634, 1683
Msp20I	TGGCCA	1 cut(s)	1634
MspI	CCGG	2 cut(s)	551, 1597
MspR9I	CCNGG	5 cut(s)	551, 552, 732, 1597, 1617
MunI	CAATTG	1 cut(s)	1095
Mva1269I	GAATGC	2 cut(s)	237, 892
MvaI	CCWGG	2 cut(s)	732, 1617
MvnI	CGCG	1 cut(s)	185
MwoI	GCNNNNNNNGC	2 cut(s)	287, 641
NciI	CCSGG	3 cut(s)	551, 552, 1597
NcoI	CCATGG	1 cut(s)	1635
NdeII	GATC	4 cut(s)	322, 676, 697, 847
NlaIII	CATG	7 cut(s)	245, 539, 1510, 1538, 1633, 1639, 1688
NlaIV	GGNNCC	3 cut(s)	63, 777, 1033
NmeAIII	GCCGAG	1 cut(s)	8
NsiI	ATGCAT	1 cut(s)	1693
NspI	RCATGY	2 cut(s)	539, 1538
NspV	TTCGAA	1 cut(s)	685
PciI	ACATGT	1 cut(s)	535
PciSI	GCTCTTC	2 cut(s)	310, 704
PctI	GAATGC	2 cut(s)	237, 892
PfeI	GAWTC	4 cut(s)	575, 611, 1085, 1478
PkrI	GCNGC	3 cut(s)	937, 1354, 1648
PpuMI	RGGWCCY	1 cut(s)	1031
PscI	ACATGT	1 cut(s)	535
PshAI	GACNNNNGTC	1 cut(s)	1611
Psp124BI	GAGCTC	1 cut(s)	1229
Psp5II	RGGWCCY	1 cut(s)	1031
Psp6I	CCWGG	2 cut(s)	730, 1615
PspGI	CCWGG	2 cut(s)	730, 1615
PspN4I	GGNNCC	3 cut(s)	63, 777, 1033
PspPI	GGNCC	3 cut(s)	643, 788, 1031
PspPPI	RGGWCCY	1 cut(s)	1031
PsuI	RGATCY	1 cut(s)	697
RsaI	GTAC	3 cut(s)	1093, 1327, 1667
RsaNI	GTAC	3 cut(s)	1092, 1326, 1666
RseI	CAYNNNNRTG	3 cut(s)	816, 1634, 1683
SacI	GAGCTC	1 cut(s)	1229
SapI	GCTCTTC	2 cut(s)	310, 704
SaqAI	TTAA	4 cut(s)	36, 453, 461, 540
SatI	GCNGC	3 cut(s)	936, 1353, 1647
Sau3AI	GATC	4 cut(s)	322, 676, 697, 847
Sau96I	GGNCC	3 cut(s)	643, 788, 1031
ScrFI	CCNGG	5 cut(s)	551, 552, 732, 1597, 1617
SduI	GDGCHC	3 cut(s)	532, 568, 1229
SfaNI	GCATC	4 cut(s)	277, 751, 1678, 1684
SfuI	TTCGAA	1 cut(s)	685
SinI	GGWCC	2 cut(s)	788, 1031
SmaI	CCCGGG	1 cut(s)	552
SmiMI	CAYNNNNRTG	3 cut(s)	816, 1634, 1683
SmlI	CTYRAG	1 cut(s)	1463
SmoI	CTYRAG	1 cut(s)	1463
Sse9I	AATT	6 cut(s)	796, 870, 924, 1095, 1151, 1217
SsiI	CCGC	4 cut(s)	185, 281, 1233, 1571
SspI	AATATT	2 cut(s)	442, 746
SspMI	CTAG	1 cut(s)	1089
SstI	GAGCTC	1 cut(s)	1229
StyD4I	CCNGG	5 cut(s)	549, 550, 730, 1595, 1615
StyI	CCWWGG	2 cut(s)	584, 1635
TaaI	ACNGT	6 cut(s)	726, 920, 1347, 1367, 1432, 1552
TaqI	TCGA	5 cut(s)	685, 850, 897, 1434, 1502
TasI	AATT	6 cut(s)	796, 870, 924, 1095, 1151, 1217
TatI	WGTACW	3 cut(s)	1091, 1325, 1665
TfiI	GAWTC	4 cut(s)	575, 611, 1085, 1478
Tru1I	TTAA	4 cut(s)	36, 453, 461, 540
Tru9I	TTAA	4 cut(s)	36, 453, 461, 540
TscAI	CASTG	7 cut(s)	255, 297, 807, 1170, 1259, 1321, 1372
TseI	GCWGC	3 cut(s)	935, 1352, 1646
TspGWI	ACGGA	1 cut(s)	572
TspMI	CCCGGG	1 cut(s)	550
TspRI	CASTG	7 cut(s)	255, 297, 807, 1170, 1259, 1321, 1372
VpaK11BI	GGWCC	2 cut(s)	788, 1031
XagI	CCTNNNNNAGG	1 cut(s)	1620
XapI	RAATTY	1 cut(s)	796
XceI	RCATGY	2 cut(s)	539, 1538
XmaI	CCCGGG	1 cut(s)	550
XspI	CTAG	1 cut(s)	1089
Zsp2I	ATGCAT	1 cut(s)	1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.