Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001794

Physical Location & Seq

Genomic Coordinates Forward (+)

18824717 .. 18830729

Gene Structure 6013 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g22940.t8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGAGGAGTCTCTGCGAATTCAAAGAGAGGAGGACGTTCCTTCTGCCATAGACTTCACCGCTCGAATTGAATCCAGGAATATTCCGGCAGTTATCAATGGCTGCGTCAAAGACTGGGAAGCTGTATCGAAGTGGAATCCAACGAACGGCGGCCTGGATTATTTGCAGGAACGAGTCGGATTGTGTGTTGTGGAGGCTATGCTGTCTAGCTTCGCACCTGTTTTCTACGGAGATCTTAGAAGTCATGAGAGGGTACCGCTGCCTTTTTCTGCCTTCATTCGTTTATGTAAACAACGGATGCATCATGCAGAGGATGGTTCTGGTGCTTGTGTTGAATCAGAACGGACTGGGCTGCTAGGGCCTGAATCTGAGCATGACTGTAAGCCCTATGAGGATGCTCCTCAACAAATCTATTTAGCACAGGTACCGATAATCAGTACTGAAAATGAAGAGAGGGCTCAGTTGGGAACTTTGAGGGAAGATATTCAAATGCCTTCATTTTTGGAGGATAAATCACTAATTTCCGTCAACCTCTGGATGAACAATGCTCAAGCTAGATCAAGCACTCACTATGATCCACACCATAACCTTCTCTGTGTAGTTTCTGGCTGCAAACAAGTGGTTTTATGGCCTCCTTCTGCAACTCCTGTGTTATACCCTATGCCAATATATGGGGAGGCATCAAATCATAGTTCTGTTCCTTTAGAGAACTTTGACTTTTCAAATTACCCAAGAGCAGAATGTTCAAGGGAGTACTCGCAAAAGGTTATTCTTCATGCAGGGGATGCACTATTTATTCCTGAAGGCTGGTTCCACCAGGTAGACAGTGATAAGCTGACAATTGCTGTTAACTTTTGGTGGCAATCAAAGATCATGTCATGCATGACAGAACACATGGATGCATATTATTTGCGCATACTGTTAAGAAGAATGACAATTCGAGAAATGAACCAAGTGCTGCCTAAAGGTTCTTCAGAGGAAATGGCGAACACAACGAGGCATGTGCTTAATAACGGGCAAGTAGATCACAATGACAAAGGTTTGGATCAGGCAAGTGAGAAGGAGGATCTTGAAGGAAAGGAGCTTAAGCAAGAAATTACCTTGCACGAACTGGAACCAGTTGCCGTCCATGCTCTTCGTGAACTCATTTCTCTAGTTCACAGTCACGTCAATCCTGCAGAGCAAAGACAACCGGAGCAATCCACTTCAACAACTGATTCTGCAATCAGTGTCAAGGACAAGGGTAACAAAGTTATGAGATCGGATGCATTTAGGTTGGAAGATGACCCTGTGGCTAACATTATTTGGACTCTTCCGCCGTGCACTCTTCAACGTGTACTTCTTGCCATGGTGAAAAATTTCCCAAGAACTTTGGAGGCTCTAATACTGCACATGCTTACACCGGTGGGAGCAGAAGTTCTTACACGTAAATTTGAAGAAATGGATGAACTGAACACCAAGGAAGATCGCAACAGATTCTACCAGGTTTTCTATGAATCATTCAATGACCAGTTTGCTGCAATGGATGCAATTCTGAATGGAAAGGAATTATTTGCCCTCCAGGCATACAAGAATGTGTTAGATAAGAACATAGGAGTAGATTGGGGTGGGCCAAAATCCGGTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016491 GO:0016569 GO:0016570 GO:0016577 GO:0016705 GO:0016706 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032451 GO:0032452 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_8

Protein Analysis

543

Amino Acids

61.54

Weight (kDa)

5.19

Isoelectric Point (pI)

54.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_8	PF13621	81 - 224	1.4e-42	Cupin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26135 AT5G19840 AT5G19840
fragaria_vesca	FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940
malus_domestica	MD03G1258000.v1.1 MD03G1258100.v1.1 MD11G1278700.v1.1
prunus_persica	Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1
pyrus_communis	pycom03g20440 pycom11g24620 pycom11g24630
rosa_chinensis	RchiOBHm_Chr6g0289901 RchiOBHm_Chr6g0289911 RchiOBHm_Chr6g0289951
rosa_laevigata	RLG00000012257
rosa_multiflora	Rmu_sc0001312.1_g000002 Rmu_sc0023926.1_g000002
rosa_roxburghii	Rroxscaffold_7G00177480
rosa_rugosa	Rorug06G0208000
rosa_samantha	Rh6AG319300 Rh6BG326300 Rh6BG326400 Rh6CG332300 Rh6DG318000 Rh6DG318100
rosa_wichuraiana	Rw0G009780 Rw0G009800 Rw6G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	914
Acc65I	GGTACC	2 cut(s)	253, 424
AccB1I	GGYRCC	2 cut(s)	253, 424
AccB7I	CCANNNNNTGG	1 cut(s)	671
AccBSI	CCGCTC	1 cut(s)	62
AccI	GTMKAC	1 cut(s)	822
AciI	CCGC	4 cut(s)	60, 150, 257, 1316
AclWI	GGATC	3 cut(s)	569, 1053, 1074
AcsI	RAATTY	3 cut(s)	18, 1357, 1430
AcuI	CTGAAG	2 cut(s)	822, 957
AfaI	GTAC	5 cut(s)	255, 426, 439, 755, 1338
AfiI	CCNNNNNNNGG	3 cut(s)	146, 671, 1619
AflII	CTTAAG	1 cut(s)	1085
AflIII	ACRYGT	2 cut(s)	1333, 1424
AgeI	ACCGGT	1 cut(s)	1402
AjiI	CACGTC	1 cut(s)	1168
AjnI	CCWGG	5 cut(s)	74, 153, 816, 1482, 1560
AloI	GAACNNNNNNTCC	2 cut(s)	1401, 1433
AluBI	AGCT	5 cut(s)	122, 210, 554, 835, 1084
AluI	AGCT	5 cut(s)	122, 210, 554, 835, 1084
Alw21I	GWGCWC	1 cut(s)	1325
Alw26I	GTCTC	1 cut(s)	15
Alw44I	GTGCAC	1 cut(s)	1321
AlwI	GGATC	3 cut(s)	569, 1053, 1074
AoxI	GGCC	4 cut(s)	151, 359, 629, 1610
ApaLI	GTGCAC	1 cut(s)	1321
ApeKI	GCWGC	6 cut(s)	102, 259, 352, 609, 958, 1517
ApoI	RAATTY	3 cut(s)	18, 1357, 1430
ArsI	GACNNNNNNTTYG	2 cut(s)	860, 892
AsiGI	ACCGGT	1 cut(s)	1402
Asp700I	GAANNNNTTC	2 cut(s)	483, 1358
Asp718I	GGTACC	2 cut(s)	253, 424
AspLEI	GCGC	1 cut(s)	915
AspS9I	GGNCC	2 cut(s)	359, 1610
AsuHPI	GGTGA	2 cut(s)	49, 1363
BaeGI	GKGCMC	1 cut(s)	1325
BanI	GGYRCC	2 cut(s)	253, 424
BanII	GRGCYC	1 cut(s)	460
Bbv12I	GWGCWC	1 cut(s)	1325
BbvI	GCAGC	6 cut(s)	89, 246, 339, 596, 945, 1504
BccI	CCATC	1 cut(s)	308
BceAI	ACGGC	3 cut(s)	163, 1109, 1303
BcgI	CGANNNNNNTGC	2 cut(s)	985, 1019
BciT130I	CCWGG	5 cut(s)	76, 155, 818, 1484, 1562
BcoDI	GTCTC	1 cut(s)	15
BfaI	CTAG	4 cut(s)	207, 356, 555, 1154
BfmI	CTRYAG	1 cut(s)	1176
BfrI	CTTAAG	1 cut(s)	1085
BglI	GCCNNNNNGGC	1 cut(s)	1562
BglII	AGATCT	1 cut(s)	232
BisI	GCNGC	7 cut(s)	103, 151, 260, 353, 610, 959, 1518
BlsI	GCNGC	7 cut(s)	104, 152, 261, 354, 611, 960, 1519
BmcAI	AGTACT	2 cut(s)	439, 755
Bme1390I	CCNGG	5 cut(s)	76, 155, 818, 1484, 1562
BmgBI	CACGTC	1 cut(s)	1168
BmgT120I	GGNCC	2 cut(s)	359, 1610
BmiI	GGNNCC	4 cut(s)	255, 426, 812, 1116
BmrFI	CCNGG	5 cut(s)	76, 155, 818, 1484, 1562
BmrI	ACTGGG	2 cut(s)	124, 357
BmsI	GCATC	8 cut(s)	288, 310, 385, 689, 775, 889, 1255, 1516
BmuI	ACTGGG	2 cut(s)	124, 357
BpmI	CTGGAG	1 cut(s)	1544
BpuEI	CTTGAG	1 cut(s)	534
BsaAI	YACGTR	1 cut(s)	1427
BsaJI	CCNNGG	2 cut(s)	1347, 1458
BsaWI	WCCGGW	3 cut(s)	1192, 1402, 1619
BsaXI	ACNNNNNCTCC	2 cut(s)	1401, 1431
Bsc4I	CCNNNNNNNGG	3 cut(s)	146, 671, 1619
Bse118I	RCCGGY	1 cut(s)	1402
Bse1I	ACTGG	5 cut(s)	119, 352, 1116, 1118, 1510
Bse3DI	GCAATG	1 cut(s)	1527
BseBI	CCWGG	5 cut(s)	76, 155, 818, 1484, 1562
BseDI	CCNNGG	2 cut(s)	1347, 1458
BseGI	GGATG	9 cut(s)	303, 319, 400, 543, 790, 904, 1270, 1450, 1531
BseLI	CCNNNNNNNGG	3 cut(s)	146, 671, 1619
BseMI	GCAATG	1 cut(s)	1527
BseMII	CTCAG	2 cut(s)	360, 473
BseNI	ACTGG	5 cut(s)	119, 352, 1116, 1118, 1510
BseRI	GAGGAG	3 cut(s)	20, 44, 390
BseSI	GKGCMC	1 cut(s)	1325
BseXI	GCAGC	6 cut(s)	89, 246, 339, 596, 945, 1504
BsgI	GTGCAG	1 cut(s)	1373
BshFI	GGCC	4 cut(s)	153, 361, 631, 1612
BshNI	GGYRCC	2 cut(s)	253, 424
BshTI	ACCGGT	1 cut(s)	1402
BsiHKAI	GWGCWC	1 cut(s)	1325
BsiSI	CCGG	4 cut(s)	86, 1193, 1403, 1620
BslI	CCNNNNNNNGG	3 cut(s)	146, 671, 1619
BsmAI	GTCTC	1 cut(s)	15
BsnI	GGCC	4 cut(s)	153, 361, 631, 1612
Bsp1286I	GDGCHC	2 cut(s)	460, 1325
Bsp143I	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
Bsp19I	CCATGG	1 cut(s)	1347
BspACI	CCGC	4 cut(s)	60, 150, 257, 1316
BspANI	GGCC	4 cut(s)	153, 361, 631, 1612
BspCNI	CTCAG	2 cut(s)	361, 472
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	4 cut(s)	255, 426, 812, 1116
BspMAI	CTGCAG	1 cut(s)	1180
BspPI	GGATC	3 cut(s)	569, 1053, 1074
BspQI	GCTCTTC	1 cut(s)	1140
BspT107I	GGYRCC	2 cut(s)	253, 424
BspTI	CTTAAG	1 cut(s)	1085
BsrBI	CCGCTC	1 cut(s)	62
BsrDI	GCAATG	1 cut(s)	1527
BsrFI	RCCGGY	1 cut(s)	1402
BsrI	ACTGG	5 cut(s)	119, 352, 1116, 1118, 1510
BssAI	RCCGGY	1 cut(s)	1402
BssECI	CCNNGG	2 cut(s)	1347, 1458
BssMI	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
BssT1I	CCWWGG	2 cut(s)	1347, 1458
Bst2UI	CCWGG	5 cut(s)	76, 155, 818, 1484, 1562
Bst4CI	ACNGT	4 cut(s)	380, 827, 921, 1163
Bst6I	CTCTTC	4 cut(s)	444, 1140, 1317, 1332
BstAFI	CTTAAG	1 cut(s)	1085
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1526
BstBAI	YACGTR	1 cut(s)	1427
BstDEI	CTNAG	3 cut(s)	236, 369, 459
BstDSI	CCRYGG	1 cut(s)	1347
BstF5I	GGATG	9 cut(s)	303, 319, 400, 543, 790, 904, 1270, 1450, 1531
BstHHI	GCGC	1 cut(s)	915
BstKTI	GATC	9 cut(s)	235, 560, 577, 873, 1027, 1048, 1069, 1262, 1468
BstMAI	GTCTC	1 cut(s)	15
BstMBI	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
BstMWI	GCNNNNNNNGC	5 cut(s)	358, 785, 1130, 1526, 1562
BstNI	CCWGG	5 cut(s)	76, 155, 818, 1484, 1562
BstNSI	RCATGY	2 cut(s)	1004, 1396
BstSCI	CCNGG	5 cut(s)	74, 153, 816, 1482, 1560
BstSFI	CTRYAG	1 cut(s)	1176
BstSLI	GKGCMC	1 cut(s)	1325
BstV1I	GCAGC	6 cut(s)	89, 246, 339, 596, 945, 1504
BstX2I	RGATCY	2 cut(s)	232, 1066
BstYI	RGATCY	2 cut(s)	232, 1066
BsuRI	GGCC	4 cut(s)	153, 361, 631, 1612
BtgI	CCRYGG	1 cut(s)	1347
BtrI	CACGTC	1 cut(s)	1168
BtsCI	GGATG	9 cut(s)	303, 319, 400, 543, 790, 904, 1270, 1450, 1531
BtsIMutI	CAGTG	2 cut(s)	832, 1234
CciI	TCATGA	1 cut(s)	244
CfoI	GCGC	1 cut(s)	915
Cfr10I	RCCGGY	1 cut(s)	1402
Cfr13I	GGNCC	2 cut(s)	359, 1610
CseI	GACGC	1 cut(s)	94
CsiI	ACCWGGT	2 cut(s)	816, 1482
Csp6I	GTAC	5 cut(s)	254, 425, 438, 754, 1337
CspAI	ACCGGT	1 cut(s)	1402
CviQI	GTAC	5 cut(s)	254, 425, 438, 754, 1337
DdeI	CTNAG	3 cut(s)	236, 369, 459
DpnI	GATC	9 cut(s)	234, 559, 576, 872, 1026, 1047, 1068, 1261, 1467
DpnII	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
Eam1104I	CTCTTC	4 cut(s)	444, 1140, 1317, 1332
EarI	CTCTTC	4 cut(s)	444, 1140, 1317, 1332
EciI	GGCGGA	1 cut(s)	1305
Eco130I	CCWWGG	2 cut(s)	1347, 1458
Eco24I	GRGCYC	1 cut(s)	460
Eco57I	CTGAAG	2 cut(s)	822, 957
EcoO109I	RGGNCCY	1 cut(s)	359
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	5 cut(s)	74, 153, 816, 1482, 1560
EcoT14I	CCWWGG	2 cut(s)	1347, 1458
EcoT22I	ATGCAT	4 cut(s)	303, 884, 904, 1270
EcoT38I	GRGCYC	1 cut(s)	460
ErhI	CCWWGG	2 cut(s)	1347, 1458
FblI	GTMKAC	1 cut(s)	822
Fnu4HI	GCNGC	7 cut(s)	103, 151, 260, 353, 610, 959, 1518
FokI	GGATG	9 cut(s)	310, 326, 407, 550, 797, 911, 1277, 1457, 1538
FriOI	GRGCYC	1 cut(s)	460
Fsp4HI	GCNGC	7 cut(s)	103, 151, 260, 353, 610, 959, 1518
FspBI	CTAG	4 cut(s)	207, 356, 555, 1154
FspI	TGCGCA	1 cut(s)	914
GlaI	GCGC	1 cut(s)	914
GluI	GCNGC	7 cut(s)	103, 151, 260, 353, 610, 959, 1518
GsuI	CTGGAG	1 cut(s)	1544
HaeIII	GGCC	4 cut(s)	153, 361, 631, 1612
HapII	CCGG	4 cut(s)	86, 1193, 1403, 1620
HgaI	GACGC	1 cut(s)	94
HhaI	GCGC	1 cut(s)	915
Hin6I	GCGC	1 cut(s)	913
HinP1I	GCGC	1 cut(s)	913
HincII	GTYRAC	2 cut(s)	529, 850
HindII	GTYRAC	2 cut(s)	529, 850
HpaI	GTTAAC	1 cut(s)	850
HpaII	CCGG	4 cut(s)	86, 1193, 1403, 1620
HphI	GGTGA	2 cut(s)	49, 1363
Hpy166II	GTNNAC	8 cut(s)	290, 529, 823, 850, 1142, 1159, 1323, 1337
Hpy188I	TCNGA	6 cut(s)	179, 340, 370, 976, 1264, 1536
Hpy188III	TCNNGA	6 cut(s)	245, 535, 800, 941, 1070, 1139
Hpy8I	GTNNAC	8 cut(s)	290, 529, 823, 850, 1142, 1159, 1323, 1337
HpyAV	CCTTC	8 cut(s)	51, 283, 504, 599, 645, 797, 1054, 1067
HpyCH4III	ACNGT	4 cut(s)	380, 827, 921, 1163
HpyCH4IV	ACGT	4 cut(s)	36, 1167, 1333, 1426
HpyF10VI	GCNNNNNNNGC	5 cut(s)	358, 785, 1130, 1526, 1562
HpyF3I	CTNAG	3 cut(s)	236, 369, 459
HpySE526I	ACGT	4 cut(s)	36, 1167, 1333, 1426
HspAI	GCGC	1 cut(s)	913
KpnI	GGTACC	2 cut(s)	257, 428
KspAI	GTTAAC	1 cut(s)	850
Kzo9I	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	4 cut(s)	403, 1081, 1195, 1409
Lsp1109I	GCAGC	6 cut(s)	89, 246, 339, 596, 945, 1504
LweI	GCATC	8 cut(s)	288, 310, 385, 689, 775, 889, 1255, 1516
MabI	ACCWGGT	2 cut(s)	816, 1482
MaeI	CTAG	4 cut(s)	207, 356, 555, 1154
MaeII	ACGT	4 cut(s)	36, 1167, 1333, 1426
MaeIII	GTNAC	2 cut(s)	1163, 1244
MalI	GATC	9 cut(s)	234, 559, 576, 872, 1026, 1047, 1068, 1261, 1467
MbiI	CCGCTC	1 cut(s)	62
MboI	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
MfeI	CAATTG	1 cut(s)	840
MflI	RGATCY	2 cut(s)	232, 1066
MhlI	GDGCHC	2 cut(s)	460, 1325
MlyI	GAGTC	3 cut(s)	17, 183, 1303
MmeI	TCCRAC	3 cut(s)	157, 164, 1257
Mph1103I	ATGCAT	4 cut(s)	303, 884, 904, 1270
MroXI	GAANNNNTTC	2 cut(s)	483, 1358
MseI	TTAA	4 cut(s)	849, 923, 1008, 1086
MslI	CAYNNNNRTG	1 cut(s)	897
MspA1I	CMGCKG	1 cut(s)	259
MspCI	CTTAAG	1 cut(s)	1085
MspI	CCGG	4 cut(s)	86, 1193, 1403, 1620
MspR9I	CCNGG	5 cut(s)	76, 155, 818, 1484, 1562
MunI	CAATTG	1 cut(s)	840
MvaI	CCWGG	5 cut(s)	76, 155, 818, 1484, 1562
MwoI	GCNNNNNNNGC	5 cut(s)	358, 785, 1130, 1526, 1562
NcoI	CCATGG	1 cut(s)	1347
NdeII	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
NlaIV	GGNNCC	4 cut(s)	255, 426, 812, 1116
NmuCI	GTSAC	1 cut(s)	1163
NsbI	TGCGCA	1 cut(s)	914
NsiI	ATGCAT	4 cut(s)	303, 884, 904, 1270
NspI	RCATGY	2 cut(s)	1004, 1396
PagI	TCATGA	1 cut(s)	244
PciSI	GCTCTTC	1 cut(s)	1140
PdmI	GAANNNNTTC	2 cut(s)	483, 1358
PfeI	GAWTC	7 cut(s)	71, 136, 335, 365, 1217, 1476, 1496
PflMI	CCANNNNNTGG	1 cut(s)	671
PfoI	TCCNGGA	1 cut(s)	74
PinAI	ACCGGT	1 cut(s)	1402
PkrI	GCNGC	7 cut(s)	104, 152, 261, 354, 611, 960, 1519
PleI	GAGTC	3 cut(s)	16, 182, 1303
PpsI	GAGTC	3 cut(s)	16, 182, 1303
Ppu21I	YACGTR	1 cut(s)	1427
Psp6I	CCWGG	5 cut(s)	74, 153, 816, 1482, 1560
PspGI	CCWGG	5 cut(s)	74, 153, 816, 1482, 1560
PspN4I	GGNNCC	4 cut(s)	255, 426, 812, 1116
PspPI	GGNCC	2 cut(s)	359, 1610
PstI	CTGCAG	1 cut(s)	1180
PsuI	RGATCY	2 cut(s)	232, 1066
RsaI	GTAC	5 cut(s)	255, 426, 439, 755, 1338
RsaNI	GTAC	5 cut(s)	254, 425, 438, 754, 1337
RseI	CAYNNNNRTG	1 cut(s)	897
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	4 cut(s)	849, 923, 1008, 1086
SatI	GCNGC	7 cut(s)	103, 151, 260, 353, 610, 959, 1518
Sau3AI	GATC	9 cut(s)	232, 557, 574, 870, 1024, 1045, 1066, 1259, 1465
Sau96I	GGNCC	2 cut(s)	359, 1610
ScaI	AGTACT	2 cut(s)	439, 755
SchI	GAGTC	3 cut(s)	17, 183, 1303
ScrFI	CCNGG	5 cut(s)	76, 155, 818, 1484, 1562
SduI	GDGCHC	2 cut(s)	460, 1325
SexAI	ACCWGGT	2 cut(s)	816, 1482
SfaNI	GCATC	8 cut(s)	288, 310, 385, 689, 775, 889, 1255, 1516
SfcI	CTRYAG	1 cut(s)	1176
SgrAI	CRCCGGYG	1 cut(s)	1402
SmiMI	CAYNNNNRTG	1 cut(s)	897
SmlI	CTYRAG	2 cut(s)	549, 1085
SmoI	CTYRAG	2 cut(s)	549, 1085
SsiI	CCGC	4 cut(s)	60, 150, 257, 1316
SspI	AATATT	1 cut(s)	82
SspMI	CTAG	4 cut(s)	207, 356, 555, 1154
StyD4I	CCNGG	5 cut(s)	74, 153, 816, 1482, 1560
StyI	CCWWGG	2 cut(s)	1347, 1458
TaaI	ACNGT	4 cut(s)	380, 827, 921, 1163
TaiI	ACGT	4 cut(s)	39, 1170, 1336, 1429
TaqI	TCGA	3 cut(s)	64, 128, 940
TatI	WGTACW	3 cut(s)	437, 753, 1336
TauI	GCSGC	1 cut(s)	153
TfiI	GAWTC	7 cut(s)	71, 136, 335, 365, 1217, 1476, 1496
Tru1I	TTAA	4 cut(s)	849, 923, 1008, 1086
Tru9I	TTAA	4 cut(s)	849, 923, 1008, 1086
TscAI	CASTG	2 cut(s)	832, 1234
TseFI	GTSAC	1 cut(s)	1163
TseI	GCWGC	6 cut(s)	102, 259, 352, 609, 958, 1517
Tsp45I	GTSAC	1 cut(s)	1163
TspDTI	ATGAA	8 cut(s)	265, 462, 486, 554, 764, 962, 1461, 1509
TspGWI	ACGGA	4 cut(s)	243, 310, 358, 514
TspRI	CASTG	2 cut(s)	832, 1234
Van91I	CCANNNNNTGG	1 cut(s)	671
Vha464I	CTTAAG	1 cut(s)	1085
VneI	GTGCAC	1 cut(s)	1321
XapI	RAATTY	3 cut(s)	18, 1357, 1430
XceI	RCATGY	2 cut(s)	1004, 1396
XmiI	GTMKAC	1 cut(s)	822
XmnI	GAANNNNTTC	2 cut(s)	483, 1358
XspI	CTAG	4 cut(s)	207, 356, 555, 1154
ZrmI	AGTACT	2 cut(s)	439, 755
Zsp2I	ATGCAT	4 cut(s)	303, 884, 904, 1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.