Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001794

Physical Location & Seq

Genomic Coordinates Reverse (-)

16812691 .. 16817943

Gene Structure 5253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012257

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGAGGAGTCTCTGCGAATTCGAAGATTGGAGGAAGTTCCTCCGGCCAAAGACTTCACCGCTCGGATTGAATCCAGGAATATTCCGGCGGTTATCAAGGGCTGCGTTAAAGATTGGGAAGCTGTTTCCAAGTGGAATCCATCGAATGGCGGCCTGGAATATTTGCAGGAACGAGTCGGATTGTGTGTTGTGGAGGCTATGCTATCTAGTTTCGCACCTGTCTTCTACGGTGATCTTAGAAGTCATGAGAGGGTACCACTGCCATTTTCTGCCTTTATTCGTCTATGTAGACTACGCATGCATCATGCAGAGGATGGGTCTGGTGCTTATTCTGAATCGGAACGGACTGGGCTGCTAGGGCCTGAATCTGAGCATGACTGTAAGCCCTACGAGGATGCTCCTCAACAAATCTATTTAGCACAGGTACCGATAATCAGTACTGAAAATGAAGAGAGGGTTCAGTTGGAAACTTTGAGGGAAGATATTCAAATGCCTGCATTTTTGGAGGATAAATCACTAATTTCTGTCAACCTCTGGATGAACAATGCTCAAGCTAGATCAAGCACTCACTATGATCCACACCATAACCTTCTCTGCATAGTTTCTGGCTGTAAACAAGTGGTTTTATGGCCTCCTTCAGCAACTCCGGTGTTATACCCTATGCCAATATATGGGGAGGCATCAAATCATAGTTCTGTTCCTTTAGACAACCTTGACTTTTCAAATTATCCAAGAGCAGAATCTTCAATGGAGTACTCGCAGAAGGTTATTCTTCATGCAGGGGATGCACTATTTATTCCTGAAGGCTGGTTCCACCAGGTGGATAGTGATGATCTGACAATCGCTGTCAACTTTTGGTGGCAATCAAACATAATGTCATGCTTGTCAGAACACATGGATGCATATTATTTGCGCGTACTATTAAGAAGTGTCAAGGACAAGGGTAGCAAAGTTGTGAGAGTGCATGCATTTAGGTTGGAAGATGACCCAGTCGCTAACATTATTTGGACTCTTCCGCCATGCACTCTTCAACGCGTACTTCTTGCCATGGTGAAAAGTTTCCCAAGAACTTTGGAGGCTTTAATACTGCACATGCTTACACCAGTGGGAGCAGAAGTGCTTACACGTAAATTTGAAGAAATGGATGACCTGAACACCAAGGAAGATCGGAAGAGATTCTACCAAGTTTTCTATGAATCATTCAACGACCAATTTGTTGCAATGGATGCAATTCTGAATGGAAAGGAATTATTTGCCCTCCAGGCATACAAGAATGTGTTAGATAAGAACATAGGAGTAGATTGGGGTGGGCCAAAACCAGTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016491 GO:0016569 GO:0016570 GO:0016577 GO:0016705 GO:0016706 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032451 GO:0032452 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_8

Protein Analysis

443

Amino Acids

50.41

Weight (kDa)

5.27

Isoelectric Point (pI)

57.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_8	PF13621	18 - 290	7.6e-53	Cupin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26135 AT5G19840 AT5G19840
fragaria_vesca	FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940 FvH4_2g22940
malus_domestica	MD03G1258000.v1.1 MD03G1258100.v1.1 MD11G1278700.v1.1
prunus_persica	Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1 Prupe.8G234700_v2.0.a1
pyrus_communis	pycom03g20440 pycom11g24620 pycom11g24630
rosa_chinensis	RchiOBHm_Chr6g0289901 RchiOBHm_Chr6g0289911 RchiOBHm_Chr6g0289951
rosa_laevigata	RLG00000012257
rosa_multiflora	Rmu_sc0001312.1_g000002 Rmu_sc0023926.1_g000002
rosa_roxburghii	Rroxscaffold_7G00177480
rosa_rugosa	Rorug06G0208000
rosa_samantha	Rh6AG319300 Rh6BG326300 Rh6BG326400 Rh6CG332300 Rh6DG318000 Rh6DG318100
rosa_wichuraiana	Rw0G009780 Rw0G009800 Rw6G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	253, 424
AccB1I	GGYRCC	2 cut(s)	253, 424
AccB7I	CCANNNNNTGG	3 cut(s)	146, 671, 820
AccBSI	CCGCTC	1 cut(s)	62
AccI	GTMKAC	1 cut(s)	289
AccII	CGCG	2 cut(s)	915, 1035
AciI	CCGC	4 cut(s)	60, 89, 150, 1016
AclWI	GGATC	1 cut(s)	569
AcoI	YGGCCR	1 cut(s)	45
AcsI	RAATTY	2 cut(s)	18, 1130
AcuI	CTGAAG	2 cut(s)	621, 822
AdeI	CACNNNGTG	1 cut(s)	820
AfaI	GTAC	6 cut(s)	255, 426, 439, 755, 918, 1038
AfiI	CCNNNNNNNGG	3 cut(s)	146, 671, 820
AflIII	ACRYGT	2 cut(s)	1033, 1124
AgsI	TTSAA	7 cut(s)	71, 488, 723, 747, 1031, 1136, 1204
AjnI	CCWGG	4 cut(s)	74, 153, 816, 1260
AluBI	AGCT	2 cut(s)	122, 554
AluI	AGCT	2 cut(s)	122, 554
Alw26I	GTCTC	1 cut(s)	15
AlwI	GGATC	1 cut(s)	569
AoxI	GGCC	5 cut(s)	45, 151, 359, 629, 1310
ApeKI	GCWGC	2 cut(s)	102, 352
ApoI	RAATTY	2 cut(s)	18, 1130
ArsI	GACNNNNNNTTYG	2 cut(s)	860, 892
Asp700I	GAANNNNTTC	3 cut(s)	483, 1058, 1175
Asp718I	GGTACC	2 cut(s)	253, 424
AspLEI	GCGC	1 cut(s)	915
AspS9I	GGNCC	2 cut(s)	359, 1310
AsuHPI	GGTGA	3 cut(s)	49, 242, 1063
AsuII	TTCGAA	1 cut(s)	22
BanI	GGYRCC	2 cut(s)	253, 424
BarI	GAAGNNNNNNTAC	2 cut(s)	1163, 1195
BbsI	GAAGAC	1 cut(s)	214
BbvI	GCAGC	2 cut(s)	89, 339
BccI	CCATC	2 cut(s)	148, 308
BciT130I	CCWGG	4 cut(s)	76, 155, 818, 1262
BcoDI	GTCTC	1 cut(s)	15
BfaI	CTAG	3 cut(s)	207, 356, 555
BglI	GCCNNNNNGGC	1 cut(s)	1262
BisI	GCNGC	3 cut(s)	103, 151, 353
BlsI	GCNGC	3 cut(s)	104, 152, 354
BmcAI	AGTACT	2 cut(s)	439, 755
Bme1390I	CCNGG	4 cut(s)	76, 155, 818, 1262
BmgT120I	GGNCC	2 cut(s)	359, 1310
BmiI	GGNNCC	3 cut(s)	255, 426, 812
BmrFI	CCNGG	4 cut(s)	76, 155, 818, 1262
BmrI	ACTGGG	2 cut(s)	357, 983
BmsI	GCATC	6 cut(s)	310, 385, 689, 775, 889, 1216
BmuI	ACTGGG	2 cut(s)	357, 983
BpiI	GAAGAC	1 cut(s)	214
BpmI	CTGGAG	1 cut(s)	1244
Bpu14I	TTCGAA	1 cut(s)	22
BpuEI	CTTGAG	1 cut(s)	534
BsaAI	YACGTR	1 cut(s)	1127
BsaJI	CCNNGG	2 cut(s)	1047, 1158
BsaWI	WCCGGW	1 cut(s)	646
BsaXI	ACNNNNNCTCC	2 cut(s)	1101, 1131
Bsc4I	CCNNNNNNNGG	3 cut(s)	146, 671, 820
Bse1I	ACTGG	4 cut(s)	352, 989, 1103, 1319
Bse3DI	GCAATG	1 cut(s)	1227
BseBI	CCWGG	4 cut(s)	76, 155, 818, 1262
BseDI	CCNNGG	2 cut(s)	1047, 1158
BseGI	GGATG	7 cut(s)	319, 400, 543, 790, 904, 1150, 1231
BseLI	CCNNNNNNNGG	3 cut(s)	146, 671, 820
BseMI	GCAATG	1 cut(s)	1227
BseMII	CTCAG	1 cut(s)	360
BseNI	ACTGG	4 cut(s)	352, 989, 1103, 1319
BseRI	GAGGAG	2 cut(s)	20, 390
BseXI	GCAGC	2 cut(s)	89, 339
BsgI	GTGCAG	1 cut(s)	1073
Bsh1236I	CGCG	2 cut(s)	915, 1035
BshFI	GGCC	5 cut(s)	47, 153, 361, 631, 1312
BshNI	GGYRCC	2 cut(s)	253, 424
BsiSI	CCGG	3 cut(s)	44, 86, 647
BslI	CCNNNNNNNGG	3 cut(s)	146, 671, 820
BsmAI	GTCTC	1 cut(s)	15
BsnI	GGCC	5 cut(s)	47, 153, 361, 631, 1312
Bsp119I	TTCGAA	1 cut(s)	22
Bsp143I	GATC	5 cut(s)	232, 557, 574, 832, 1165
Bsp19I	CCATGG	1 cut(s)	1047
BspACI	CCGC	4 cut(s)	60, 89, 150, 1016
BspANI	GGCC	5 cut(s)	47, 153, 361, 631, 1312
BspCNI	CTCAG	1 cut(s)	361
BspFNI	CGCG	2 cut(s)	915, 1035
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	3 cut(s)	255, 426, 812
BspPI	GGATC	1 cut(s)	569
BspT104I	TTCGAA	1 cut(s)	22
BspT107I	GGYRCC	2 cut(s)	253, 424
BsrBI	CCGCTC	1 cut(s)	62
BsrDI	GCAATG	1 cut(s)	1227
BsrI	ACTGG	4 cut(s)	352, 989, 1103, 1319
BssECI	CCNNGG	2 cut(s)	1047, 1158
BssMI	GATC	5 cut(s)	232, 557, 574, 832, 1165
BssT1I	CCWWGG	2 cut(s)	1047, 1158
Bst2UI	CCWGG	4 cut(s)	76, 155, 818, 1262
Bst4CI	ACNGT	2 cut(s)	230, 380
Bst6I	CTCTTC	4 cut(s)	444, 1017, 1032, 1166
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1226
BstBAI	YACGTR	1 cut(s)	1127
BstBI	TTCGAA	1 cut(s)	22
BstC8I	GCNNGC	3 cut(s)	299, 495, 966
BstDEI	CTNAG	2 cut(s)	236, 369
BstDSI	CCRYGG	1 cut(s)	1047
BstF5I	GGATG	7 cut(s)	319, 400, 543, 790, 904, 1150, 1231
BstFNI	CGCG	2 cut(s)	915, 1035
BstHHI	GCGC	1 cut(s)	915
BstKTI	GATC	5 cut(s)	235, 560, 577, 835, 1168
BstMAI	GTCTC	1 cut(s)	15
BstMBI	GATC	5 cut(s)	232, 557, 574, 832, 1165
BstMWI	GCNNNNNNNGC	4 cut(s)	358, 785, 1226, 1262
BstNI	CCWGG	4 cut(s)	76, 155, 818, 1262
BstNSI	RCATGY	3 cut(s)	301, 968, 1096
BstSCI	CCNGG	4 cut(s)	74, 153, 816, 1260
BstUI	CGCG	2 cut(s)	915, 1035
BstV1I	GCAGC	2 cut(s)	89, 339
BstV2I	GAAGAC	1 cut(s)	214
BsuRI	GGCC	5 cut(s)	47, 153, 361, 631, 1312
BtgI	CCRYGG	1 cut(s)	1047
BtsCI	GGATG	7 cut(s)	319, 400, 543, 790, 904, 1150, 1231
BtsI	GCAGTG	1 cut(s)	257
BtsIMutI	CAGTG	2 cut(s)	257, 1110
Cac8I	GCNNGC	3 cut(s)	299, 495, 966
CciI	TCATGA	1 cut(s)	244
CfoI	GCGC	1 cut(s)	915
Cfr13I	GGNCC	2 cut(s)	359, 1310
CsiI	ACCWGGT	1 cut(s)	816
Csp6I	GTAC	6 cut(s)	254, 425, 438, 754, 917, 1037
CviQI	GTAC	6 cut(s)	254, 425, 438, 754, 917, 1037
DdeI	CTNAG	2 cut(s)	236, 369
DpnI	GATC	5 cut(s)	234, 559, 576, 834, 1167
DpnII	GATC	5 cut(s)	232, 557, 574, 832, 1165
DraIII	CACNNNGTG	1 cut(s)	820
EaeI	YGGCCR	1 cut(s)	45
Eam1104I	CTCTTC	4 cut(s)	444, 1017, 1032, 1166
EarI	CTCTTC	4 cut(s)	444, 1017, 1032, 1166
EciI	GGCGGA	1 cut(s)	1005
Eco130I	CCWWGG	2 cut(s)	1047, 1158
Eco57I	CTGAAG	2 cut(s)	621, 822
EcoO109I	RGGNCCY	1 cut(s)	359
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	4 cut(s)	74, 153, 816, 1260
EcoT14I	CCWWGG	2 cut(s)	1047, 1158
EcoT22I	ATGCAT	3 cut(s)	303, 904, 970
ErhI	CCWWGG	2 cut(s)	1047, 1158
FblI	GTMKAC	1 cut(s)	289
Fnu4HI	GCNGC	3 cut(s)	103, 151, 353
FokI	GGATG	7 cut(s)	326, 407, 550, 797, 911, 1157, 1238
Fsp4HI	GCNGC	3 cut(s)	103, 151, 353
FspBI	CTAG	3 cut(s)	207, 356, 555
GlaI	GCGC	1 cut(s)	914
GluI	GCNGC	3 cut(s)	103, 151, 353
GsuI	CTGGAG	1 cut(s)	1244
HaeIII	GGCC	5 cut(s)	47, 153, 361, 631, 1312
HapII	CCGG	3 cut(s)	44, 86, 647
HhaI	GCGC	1 cut(s)	915
Hin6I	GCGC	1 cut(s)	913
HinP1I	GCGC	1 cut(s)	913
HincII	GTYRAC	2 cut(s)	529, 850
HindII	GTYRAC	2 cut(s)	529, 850
HpaII	CCGG	3 cut(s)	44, 86, 647
HphI	GGTGA	3 cut(s)	49, 242, 1063
Hpy166II	GTNNAC	4 cut(s)	290, 529, 614, 850
Hpy188I	TCNGA	9 cut(s)	66, 179, 334, 340, 370, 837, 889, 1170, 1236
Hpy188III	TCNNGA	3 cut(s)	245, 535, 800
Hpy8I	GTNNAC	4 cut(s)	290, 529, 614, 850
HpyAV	CCTTC	4 cut(s)	599, 645, 757, 797
HpyCH4III	ACNGT	2 cut(s)	230, 380
HpyCH4IV	ACGT	1 cut(s)	1126
HpyF10VI	GCNNNNNNNGC	4 cut(s)	358, 785, 1226, 1262
HpyF3I	CTNAG	2 cut(s)	236, 369
HpySE526I	ACGT	1 cut(s)	1126
HspAI	GCGC	1 cut(s)	913
KpnI	GGTACC	2 cut(s)	257, 428
Kzo9I	GATC	5 cut(s)	232, 557, 574, 832, 1165
LmnI	GCTCC	2 cut(s)	403, 1109
Lsp1109I	GCAGC	2 cut(s)	89, 339
LweI	GCATC	6 cut(s)	310, 385, 689, 775, 889, 1216
MabI	ACCWGGT	1 cut(s)	816
MaeI	CTAG	3 cut(s)	207, 356, 555
MaeII	ACGT	1 cut(s)	1126
MalI	GATC	5 cut(s)	234, 559, 576, 834, 1167
MbiI	CCGCTC	1 cut(s)	62
MboI	GATC	5 cut(s)	232, 557, 574, 832, 1165
MluCI	AATT	7 cut(s)	18, 519, 724, 1130, 1211, 1230, 1247
MluI	ACGCGT	1 cut(s)	1033
MlyI	GAGTC	3 cut(s)	17, 183, 1003
MmeI	TCCRAC	3 cut(s)	157, 444, 957
Mph1103I	ATGCAT	3 cut(s)	303, 904, 970
MroXI	GAANNNNTTC	3 cut(s)	483, 1058, 1175
MseI	TTAA	3 cut(s)	108, 923, 1082
MslI	CAYNNNNRTG	1 cut(s)	897
MspI	CCGG	3 cut(s)	44, 86, 647
MspR9I	CCNGG	4 cut(s)	76, 155, 818, 1262
MvaI	CCWGG	4 cut(s)	76, 155, 818, 1262
MvnI	CGCG	2 cut(s)	915, 1035
MwoI	GCNNNNNNNGC	4 cut(s)	358, 785, 1226, 1262
NcoI	CCATGG	1 cut(s)	1047
NdeII	GATC	5 cut(s)	232, 557, 574, 832, 1165
NlaIV	GGNNCC	3 cut(s)	255, 426, 812
NsiI	ATGCAT	3 cut(s)	303, 904, 970
NspI	RCATGY	3 cut(s)	301, 968, 1096
NspV	TTCGAA	1 cut(s)	22
PaeI	GCATGC	2 cut(s)	301, 968
PagI	TCATGA	1 cut(s)	244
PdmI	GAANNNNTTC	3 cut(s)	483, 1058, 1175
PfeI	GAWTC	7 cut(s)	71, 136, 335, 365, 740, 1176, 1196
PflFI	GACNNNGTC	1 cut(s)	989
PflMI	CCANNNNNTGG	3 cut(s)	146, 671, 820
PfoI	TCCNGGA	1 cut(s)	74
PkrI	GCNGC	3 cut(s)	104, 152, 354
PleI	GAGTC	3 cut(s)	16, 182, 1003
PpsI	GAGTC	3 cut(s)	16, 182, 1003
Ppu21I	YACGTR	1 cut(s)	1127
Psp6I	CCWGG	4 cut(s)	74, 153, 816, 1260
PspGI	CCWGG	4 cut(s)	74, 153, 816, 1260
PspN4I	GGNNCC	3 cut(s)	255, 426, 812
PspPI	GGNCC	2 cut(s)	359, 1310
PsyI	GACNNNGTC	1 cut(s)	989
RsaI	GTAC	6 cut(s)	255, 426, 439, 755, 918, 1038
RsaNI	GTAC	6 cut(s)	254, 425, 438, 754, 917, 1037
RseI	CAYNNNNRTG	1 cut(s)	897
SaqAI	TTAA	3 cut(s)	108, 923, 1082
SatI	GCNGC	3 cut(s)	103, 151, 353
Sau3AI	GATC	5 cut(s)	232, 557, 574, 832, 1165
Sau96I	GGNCC	2 cut(s)	359, 1310
ScaI	AGTACT	2 cut(s)	439, 755
SchI	GAGTC	3 cut(s)	17, 183, 1003
ScrFI	CCNGG	4 cut(s)	76, 155, 818, 1262
SexAI	ACCWGGT	1 cut(s)	816
SfaNI	GCATC	6 cut(s)	310, 385, 689, 775, 889, 1216
SfuI	TTCGAA	1 cut(s)	22
SmiMI	CAYNNNNRTG	1 cut(s)	897
SmlI	CTYRAG	1 cut(s)	549
SmoI	CTYRAG	1 cut(s)	549
SphI	GCATGC	2 cut(s)	301, 968
Sse9I	AATT	7 cut(s)	18, 519, 724, 1130, 1211, 1230, 1247
SsiI	CCGC	4 cut(s)	60, 89, 150, 1016
SspI	AATATT	2 cut(s)	82, 161
SspMI	CTAG	3 cut(s)	207, 356, 555
StyD4I	CCNGG	4 cut(s)	74, 153, 816, 1260
StyI	CCWWGG	2 cut(s)	1047, 1158
TaaI	ACNGT	2 cut(s)	230, 380
TaiI	ACGT	1 cut(s)	1129
TaqI	TCGA	2 cut(s)	22, 143
TasI	AATT	7 cut(s)	18, 519, 724, 1130, 1211, 1230, 1247
TatI	WGTACW	2 cut(s)	437, 753
TauI	GCSGC	1 cut(s)	153
TfiI	GAWTC	7 cut(s)	71, 136, 335, 365, 740, 1176, 1196
Tru1I	TTAA	3 cut(s)	108, 923, 1082
Tru9I	TTAA	3 cut(s)	108, 923, 1082
TscAI	CASTG	2 cut(s)	264, 1110
TseI	GCWGC	2 cut(s)	102, 352
TspDTI	ATGAA	4 cut(s)	462, 554, 764, 1209
TspGWI	ACGGA	1 cut(s)	358
TspRI	CASTG	2 cut(s)	264, 1110
Tth111I	GACNNNGTC	1 cut(s)	989
Van91I	CCANNNNNTGG	3 cut(s)	146, 671, 820
XapI	RAATTY	2 cut(s)	18, 1130
XceI	RCATGY	3 cut(s)	301, 968, 1096
XmiI	GTMKAC	1 cut(s)	289
XmnI	GAANNNNTTC	3 cut(s)	483, 1058, 1175
XspI	CTAG	3 cut(s)	207, 356, 555
ZrmI	AGTACT	2 cut(s)	439, 755
Zsp2I	ATGCAT	3 cut(s)	303, 904, 970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.