FvH4_2g25210
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003136
Physical Location & Seq
Forward (+)
20525341 .. 20536074
10734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25210.t3

Sequence Viewer

Length: 774 bp
ATGGAACCGCTATGTCAACTGCAGCAGCGCGAGTTTTGGCTACCCTCTACGAGAAAGAGCACAGTCCAAAACCAAAGCACAGTCTTTTTTCTCAAGTTCATCTGCCAAACCATACACATTTCCACGGTCATGGCCGGCATCAAAGTCCATGGAACCGTTTTCTCTACTGCAGCAGCGCGAGTTTTGGCTACCCTCTACGAGAAAGAGGTGGAATTTGAGCTTGTTCCGATAGACATGAAAGCCGGTGAACATAAGAAGGAGTCCTTCCTTGCCCTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGACGGAGATCTTCAGCTCTTTGAGTCAAGAGCAATTACACAATACATTGCCCATGAGTACGCTTCAAAGGGAACCCCGCTGATAGTCCCAGACTCGAAGAAGATGGCAATTCTATCAGTGTGGACAGAGGTGGAGGCACACAAGTTCGACCCTGCAGCCTCAAAACTGTCATATGAGCTAGCAATAAAGCCTCTCCTTGGCATGGTCACTGACTTCGCAGTCGTGGAGGAATTCGAAGCTAAGTTGGGTACAGTTCTCGATGTCTATGAGAAACGTCTGGGACAATCGAAATATTTGGGAGGTGATTGCTTCAGCTTGGCAGATCTTCACCACCTTCCAACTACACAATACTTGATGGGAACACAAGCCAAGAAGCTCTTTGAGTGCCGTCCGAATGTTAGCGCATGGGTAGCTGATATCACTGCCAGGCCTGCTTGGACTAAAGTCCTCGCCATGAGAGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

258

Amino Acids

28.82

Weight (kDa)

6.45

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.4e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 81 3.8e-13 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 77 2.3e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 132 - 203 7.4e-13 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 132 - 197 6.7e-06 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 137 - 204 8.2e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
AccII CGCG 2 cut(s) 30, 178
AciI CCGC 2 cut(s) 8, 383
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 2 cut(s) 212, 536
AcuI CTGAAG 2 cut(s) 302, 601
AfaI GTAC 2 cut(s) 365, 556
AfiI CCNNNNNNNGG 2 cut(s) 503, 508
AgsI TTSAA 2 cut(s) 305, 372
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 9 cut(s) 220, 299, 322, 484, 545, 621, 682, 719, 767
AluI AGCT 9 cut(s) 220, 299, 322, 484, 545, 621, 682, 719, 767
Alw21I GWGCWC 2 cut(s) 62, 769
AoxI GGCC 2 cut(s) 132, 734
ApeKI GCWGC 5 cut(s) 22, 25, 170, 173, 461
ApoI RAATTY 2 cut(s) 212, 536
AspLEI GCGC 3 cut(s) 30, 178, 710
AsuHPI GGTGA 3 cut(s) 257, 620, 626
AsuII TTCGAA 1 cut(s) 540
AsuNHI GCTAGC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 62, 769
BbvI GCAGC 5 cut(s) 34, 37, 182, 185, 473
BccI CCATC 2 cut(s) 403, 655
BceAI ACGGC 1 cut(s) 678
BciT130I CCWGG 1 cut(s) 733
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 3 cut(s) 20, 168, 459
BglII AGATCT 2 cut(s) 313, 628
BisI GCNGC 5 cut(s) 23, 26, 171, 174, 462
BlsI GCNGC 5 cut(s) 24, 27, 172, 175, 463
Bme1390I CCNGG 1 cut(s) 733
BmiI GGNNCC 3 cut(s) 6, 154, 379
BmrFI CCNGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 147
BmtI GCTAGC 1 cut(s) 488
BoxI GACNNNNGTC 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 123, 148, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 508
Bse118I RCCGGY 2 cut(s) 134, 242
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 1 cut(s) 733
BseDI CCNNGG 3 cut(s) 123, 148, 502
BseLI CCNNNNNNNGG 2 cut(s) 503, 508
BseMI GCAATG 1 cut(s) 351
BseXI GCAGC 5 cut(s) 34, 37, 182, 185, 473
Bsh1236I CGCG 2 cut(s) 30, 178
BshFI GGCC 2 cut(s) 134, 736
BsiHKAI GWGCWC 2 cut(s) 62, 769
BsiSI CCGG 2 cut(s) 135, 243
BslFI GGGAC 2 cut(s) 377, 600
BslI CCNNNNNNNGG 2 cut(s) 503, 508
BsmFI GGGAC 2 cut(s) 377, 600
BsnI GGCC 2 cut(s) 134, 736
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 62, 769
Bsp143I GATC 2 cut(s) 313, 628
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 2 cut(s) 8, 383
BspANI GGCC 2 cut(s) 134, 736
BspFNI CGCG 2 cut(s) 30, 178
BspLI GGNNCC 3 cut(s) 6, 154, 379
BspMAI CTGCAG 3 cut(s) 24, 172, 463
BspOI GCTAGC 1 cut(s) 488
BspT104I TTCGAA 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 351
BsrFI RCCGGY 2 cut(s) 134, 242
BssAI RCCGGY 2 cut(s) 134, 242
BssECI CCNNGG 3 cut(s) 123, 148, 502
BssMI GATC 2 cut(s) 313, 628
BssT1I CCWWGG 2 cut(s) 148, 502
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 6 cut(s) 64, 82, 127, 157, 474, 559
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 136, 486, 738
BstDEI CTNAG 1 cut(s) 546
BstDSI CCRYGG 2 cut(s) 123, 148
BstFNI CGCG 2 cut(s) 30, 178
BstHHI GCGC 3 cut(s) 30, 178, 710
BstKTI GATC 2 cut(s) 316, 631
BstMBI GATC 2 cut(s) 313, 628
BstMWI GCNNNNNNNGC 3 cut(s) 716, 737, 764
BstNI CCWGG 1 cut(s) 733
BstPAI GACNNNNGTC 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 731
BstSFI CTRYAG 3 cut(s) 20, 168, 459
BstUI CGCG 2 cut(s) 30, 178
BstV1I GCAGC 5 cut(s) 34, 37, 182, 185, 473
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 2 cut(s) 313, 628
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 2 cut(s) 313, 628
BsuRI GGCC 2 cut(s) 134, 736
BtgI CCRYGG 2 cut(s) 123, 148
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 3 cut(s) 429, 513, 726
Cac8I GCNNGC 3 cut(s) 136, 486, 738
CfoI GCGC 3 cut(s) 30, 178, 710
Cfr10I RCCGGY 2 cut(s) 134, 242
Csp6I GTAC 2 cut(s) 364, 555
CviAII CATG 7 cut(s) 130, 149, 235, 359, 508, 711, 760
CviQI GTAC 2 cut(s) 364, 555
DdeI CTNAG 1 cut(s) 546
DpnI GATC 2 cut(s) 315, 630
DpnII GATC 2 cut(s) 313, 628
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 767
Eco130I CCWWGG 2 cut(s) 148, 502
Eco147I AGGCCT 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 769
Eco32I GATATC 1 cut(s) 724
Eco53kI GAGCTC 1 cut(s) 767
Eco57I CTGAAG 2 cut(s) 302, 601
EcoICRI GAGCTC 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 731
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 2 cut(s) 148, 502
EcoT38I GRGCYC 1 cut(s) 769
ErhI CCWWGG 2 cut(s) 148, 502
FaeI CATG 7 cut(s) 133, 152, 238, 362, 511, 714, 763
FalI AAGNNNNNCTT 4 cut(s) 248, 280, 668, 700
FaqI GGGAC 2 cut(s) 377, 600
FatI CATG 7 cut(s) 129, 148, 234, 358, 507, 710, 759
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 23, 26, 171, 174, 462
FriOI GRGCYC 1 cut(s) 769
Fsp4HI GCNGC 5 cut(s) 23, 26, 171, 174, 462
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 29, 177, 709
GluI GCNGC 5 cut(s) 23, 26, 171, 174, 462
HaeIII GGCC 2 cut(s) 134, 736
HapII CCGG 2 cut(s) 135, 243
HhaI GCGC 3 cut(s) 30, 178, 710
Hin1II CATG 7 cut(s) 133, 152, 238, 362, 511, 714, 763
Hin6I GCGC 3 cut(s) 28, 176, 708
HinP1I GCGC 3 cut(s) 28, 176, 708
HincII GTYRAC 1 cut(s) 17
HindII GTYRAC 1 cut(s) 17
HinfI GANTC 3 cut(s) 260, 329, 398
HpaII CCGG 2 cut(s) 135, 243
HphI GGTGA 3 cut(s) 257, 620, 626
Hpy166II GTNNAC 3 cut(s) 17, 248, 429
Hpy188I TCNGA 2 cut(s) 228, 699
Hpy188III TCNNGA 2 cut(s) 333, 563
Hpy8I GTNNAC 3 cut(s) 17, 248, 429
HpyAV CCTTC 3 cut(s) 250, 274, 650
HpyCH4III ACNGT 6 cut(s) 64, 82, 127, 157, 474, 559
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 3 cut(s) 22, 170, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 737, 764
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 7 cut(s) 133, 152, 238, 362, 511, 714, 763
HspAI GCGC 3 cut(s) 28, 176, 708
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 2 cut(s) 313, 628
LpnPI CCDG 9 cut(s) 148, 256, 309, 408, 471, 569, 718, 745, 750
Lsp1109I GCAGC 5 cut(s) 34, 37, 182, 185, 473
LweI GCATC 1 cut(s) 147
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 1 cut(s) 511
MalI GATC 2 cut(s) 315, 630
MboI GATC 2 cut(s) 313, 628
MboII GAAGA 5 cut(s) 308, 317, 415, 418, 623
MflI RGATCY 2 cut(s) 313, 628
MhlI GDGCHC 2 cut(s) 62, 769
MluCI AATT 4 cut(s) 212, 339, 414, 536
MlyI GAGTC 3 cut(s) 269, 338, 392
MmeI TCCRAC 1 cut(s) 668
MroNI GCCGGC 1 cut(s) 134
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 2 cut(s) 135, 243
MspR9I CCNGG 1 cut(s) 733
MvaI CCWGG 1 cut(s) 733
MvnI CGCG 2 cut(s) 30, 178
MwoI GCNNNNNNNGC 3 cut(s) 716, 737, 764
NaeI GCCGGC 1 cut(s) 136
NcoI CCATGG 1 cut(s) 148
NdeI CATATG 1 cut(s) 478
NdeII GATC 2 cut(s) 313, 628
NgoMIV GCCGGC 1 cut(s) 134
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 7 cut(s) 133, 152, 238, 362, 511, 714, 763
NlaIV GGNNCC 3 cut(s) 6, 154, 379
NmuCI GTSAC 1 cut(s) 511
NspV TTCGAA 1 cut(s) 540
PceI AGGCCT 1 cut(s) 736
PdiI GCCGGC 1 cut(s) 136
PkrI GCNGC 5 cut(s) 24, 27, 172, 175, 463
PleI GAGTC 3 cut(s) 268, 337, 392
PpsI GAGTC 3 cut(s) 268, 337, 392
PshAI GACNNNNGTC 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 769
Psp6I CCWGG 1 cut(s) 731
PspGI CCWGG 1 cut(s) 731
PspN4I GGNNCC 3 cut(s) 6, 154, 379
PstI CTGCAG 3 cut(s) 24, 172, 463
PsuI RGATCY 2 cut(s) 313, 628
RsaI GTAC 2 cut(s) 365, 556
RsaNI GTAC 2 cut(s) 364, 555
RseI CAYNNNNRTG 1 cut(s) 128
SacI GAGCTC 1 cut(s) 769
SatI GCNGC 5 cut(s) 23, 26, 171, 174, 462
Sau3AI GATC 2 cut(s) 313, 628
SchI GAGTC 3 cut(s) 269, 338, 392
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 2 cut(s) 62, 769
SfaNI GCATC 1 cut(s) 147
SfcI CTRYAG 3 cut(s) 20, 168, 459
SfuI TTCGAA 1 cut(s) 540
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 4 cut(s) 212, 339, 414, 536
SseBI AGGCCT 1 cut(s) 736
SsiI CCGC 2 cut(s) 8, 383
SspI AATATT 1 cut(s) 599
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 769
StuI AGGCCT 1 cut(s) 736
StyD4I CCNGG 1 cut(s) 731
StyI CCWWGG 2 cut(s) 148, 502
TaaI ACNGT 6 cut(s) 64, 82, 127, 157, 474, 559
TaiI ACGT 1 cut(s) 583
TaqI TCGA 5 cut(s) 401, 453, 540, 564, 593
TasI AATT 4 cut(s) 212, 339, 414, 536
TscAI CASTG 3 cut(s) 429, 520, 733
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 5 cut(s) 22, 25, 170, 173, 461
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 88, 251
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 3 cut(s) 429, 520, 733
XapI RAATTY 2 cut(s) 212, 536
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.