MD03G1282700.v1.1
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003136
Physical Location & Seq
Reverse (-)
36305324 .. 36312277
6954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1282700.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCTGCCATCAAGGTCCACGGAAACGTTATTTCGACCGCTGCAATGCGAGTTTTTGCTACTCTCTACGAGAAAGACATCGAATTCGAGCTTGTTCCAATCGACATGAGAGCTGGTGAACACAAAAAGGAGCCCTTCATATCCCTCAATCCATTTGGTCAAGTTCCAGCTTTCGAAGATGGAGATCTTAAGCTCTTTGGTAAGTTTCCATTTGGTCAAGTTCCAGCTTTCGAAGACGGAGATCTTAAGCTCTTTGAATCAAGGGCGATTACACAATACATTGCCCACGAGTATGCTGACAAGGGAACCCCTCTAGTGATCCGAGACTCAAAGAAGATGGCAATTATATCATTGTGGTCGGAGGTGGAGGCCCAAAAGTTCGACCCGGCGGCCACAAAACTGACCTATGAGCTAGCTATAAAGCCTATGTTTAAAATGACCACAGACGCGGCAGTTGTGGAGGAAAATGAAGCCAAGTTGGCTGTGGTTCTTGATGTATATGAGACTCGTCTGGCTCAGTCGAAATACTTGGCAGGTGAAAGCTTCACTTTGGCTGATCTTCACCACCTCCCCACCATACATTACTTGATGGGAACACAATCGAAGAAGCTGTTCGAATCCCGCCCCCATGTTCTCGCATGGGTGGTGGACATCACAGCAAGGCCAGCTTGGAACAAAGTCGTTGCCCAGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

233

Amino Acids

26.09

Weight (kDa)

5.99

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 4 - 66 8.9e-13 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 11 - 65 1.3e-06 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 96 1.9e-10 Glutathione S-transferase, N-terminal domain
GST_N PF02798 68 - 95 6e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 137 - 222 1.4e-16 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 155 - 223 9.1e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 524
AccII CGCG 1 cut(s) 449
AciI CCGC 4 cut(s) 39, 389, 449, 622
AclI AACGTT 1 cut(s) 27
AclWI GGATC 1 cut(s) 313
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 1 cut(s) 83
AflII CTTAAG 2 cut(s) 188, 245
AgsI TTSAA 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 318, 497
AlwI GGATC 1 cut(s) 313
AoxI GGCC 3 cut(s) 369, 390, 662
ApeKI GCWGC 2 cut(s) 5, 41
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 611
AspS9I GGNCC 2 cut(s) 16, 370
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 128, 548, 554
AsuII TTCGAA 3 cut(s) 174, 231, 615
AsuNHI GCTAGC 1 cut(s) 412
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 28
BccI CCATC 4 cut(s) 17, 173, 331, 583
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 2 cut(s) 318, 497
BfaI CTAG 2 cut(s) 314, 413
BfrI CTTAAG 2 cut(s) 188, 245
BfuAI ACCTGC 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 479
BglII AGATCT 2 cut(s) 184, 241
BisI GCNGC 4 cut(s) 6, 42, 390, 450
BlsI GCNGC 4 cut(s) 7, 43, 391, 451
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 370
BmiI GGNNCC 2 cut(s) 132, 307
BmrFI CCNGG 1 cut(s) 386
BmtI GCTAGC 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 240
Bpu14I TTCGAA 3 cut(s) 174, 231, 615
BpuMI CCSGG 1 cut(s) 386
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 19
Bse3DI GCAATG 2 cut(s) 51, 279
Bse8I GATNNNNATC 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 19
BseJI GATNNNNATC 1 cut(s) 183
BseMI GCAATG 2 cut(s) 51, 279
BseMII CTCAG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 687
Bsh1236I CGCG 1 cut(s) 449
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 371, 392, 664
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 386
BsmAI GTCTC 2 cut(s) 318, 497
BsnI GGCC 3 cut(s) 371, 392, 664
Bsp119I TTCGAA 3 cut(s) 174, 231, 615
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 4 cut(s) 184, 241, 318, 556
BspACI CCGC 4 cut(s) 39, 389, 449, 622
BspANI GGCC 3 cut(s) 371, 392, 664
BspCNI CTCAG 1 cut(s) 529
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 2 cut(s) 132, 307
BspMI ACCTGC 1 cut(s) 524
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 1 cut(s) 313
BspT104I TTCGAA 3 cut(s) 174, 231, 615
BspTI CTTAAG 2 cut(s) 188, 245
BsrDI GCAATG 2 cut(s) 51, 279
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 4 cut(s) 184, 241, 318, 556
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
BstAFI CTTAAG 2 cut(s) 188, 245
BstBI TTCGAA 3 cut(s) 174, 231, 615
BstC8I GCNNGC 2 cut(s) 414, 666
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 4 cut(s) 187, 244, 321, 559
BstMAI GTCTC 2 cut(s) 318, 497
BstMBI GATC 4 cut(s) 184, 241, 318, 556
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 2 cut(s) 479, 665
BstSCI CCNGG 1 cut(s) 384
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 2 cut(s) 184, 241
BstYI RGATCY 2 cut(s) 184, 241
BsuRI GGCC 3 cut(s) 371, 392, 664
BtgI CCRYGG 1 cut(s) 19
BveI ACCTGC 1 cut(s) 524
Cac8I GCNNGC 2 cut(s) 414, 666
Cfr13I GGNCC 2 cut(s) 16, 370
CseI GACGC 1 cut(s) 455
CviAII CATG 3 cut(s) 106, 629, 639
DdeI CTNAG 1 cut(s) 516
DpnI GATC 4 cut(s) 186, 243, 320, 558
DpnII GATC 4 cut(s) 184, 241, 318, 556
DraI TTTAAA 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 3 cut(s) 109, 632, 642
FalI AAGNNNNNCTT 6 cut(s) 119, 151, 532, 564, 652, 684
FatI CATG 3 cut(s) 105, 628, 638
FauI CCCGC 1 cut(s) 629
Fnu4HI GCNGC 4 cut(s) 6, 42, 390, 450
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 6, 42, 390, 450
FspBI CTAG 2 cut(s) 314, 413
GluI GCNGC 4 cut(s) 6, 42, 390, 450
GsaI CCCAGC 1 cut(s) 691
HaeIII GGCC 3 cut(s) 371, 392, 664
HapII CCGG 1 cut(s) 386
HgaI GACGC 1 cut(s) 455
Hin1II CATG 3 cut(s) 109, 632, 642
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 4 cut(s) 257, 326, 505, 617
HpaII CCGG 1 cut(s) 386
HphI GGTGA 3 cut(s) 128, 548, 554
Hpy166II GTNNAC 3 cut(s) 19, 119, 649
Hpy188I TCNGA 2 cut(s) 323, 361
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 3 cut(s) 19, 119, 649
HpyAV CCTTC 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 665
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 3 cut(s) 109, 632, 642
Kzo9I GATC 4 cut(s) 184, 241, 318, 556
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 7 cut(s) 99, 180, 237, 399, 497, 519, 678
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 2 cut(s) 314, 413
MaeII ACGT 1 cut(s) 27
MalI GATC 4 cut(s) 186, 243, 320, 558
MboI GATC 4 cut(s) 184, 241, 318, 556
MboII GAAGA 5 cut(s) 188, 245, 346, 551, 616
MflI RGATCY 2 cut(s) 184, 241
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 83, 342
MlyI GAGTC 2 cut(s) 320, 499
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 6 cut(s) 155, 321, 355, 361, 454, 578
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 3 cut(s) 189, 246, 432
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 2 cut(s) 188, 245
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 386
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 479, 665
NciI CCSGG 1 cut(s) 386
NdeII GATC 4 cut(s) 184, 241, 318, 556
NheI GCTAGC 1 cut(s) 412
NlaIII CATG 3 cut(s) 109, 632, 642
NlaIV GGNNCC 2 cut(s) 132, 307
NspV TTCGAA 3 cut(s) 174, 231, 615
PaqCI CACCTGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 2 cut(s) 257, 617
PkrI GCNGC 4 cut(s) 7, 43, 391, 451
PleI GAGTC 2 cut(s) 320, 499
PpsI GAGTC 2 cut(s) 320, 499
Psp1406I AACGTT 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 132, 307
PspPI GGNCC 2 cut(s) 16, 370
PsuI RGATCY 2 cut(s) 184, 241
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 3 cut(s) 189, 246, 432
SatI GCNGC 4 cut(s) 6, 42, 390, 450
Sau3AI GATC 4 cut(s) 184, 241, 318, 556
Sau96I GGNCC 2 cut(s) 16, 370
SchI GAGTC 2 cut(s) 320, 499
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 135
SfuI TTCGAA 3 cut(s) 174, 231, 615
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 188, 245
SmoI CTYRAG 2 cut(s) 188, 245
Sse9I AATT 2 cut(s) 83, 342
SsiI CCGC 4 cut(s) 39, 389, 449, 622
SspMI CTAG 2 cut(s) 314, 413
StyD4I CCNGG 1 cut(s) 384
TaiI ACGT 1 cut(s) 30
TasI AATT 2 cut(s) 83, 342
TauI GCSGC 2 cut(s) 392, 452
TfiI GAWTC 2 cut(s) 257, 617
Tru1I TTAA 3 cut(s) 189, 246, 432
Tru9I TTAA 3 cut(s) 189, 246, 432
TseI GCWGC 2 cut(s) 5, 41
TspDTI ATGAA 2 cut(s) 127, 483
TspGWI ACGGA 2 cut(s) 36, 252
Vha464I CTTAAG 2 cut(s) 188, 245
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 2 cut(s) 314, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.