pycom03g22370
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003136
Physical Location & Seq
Reverse (-)
22518852 .. 22520127
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22370.3

Sequence Viewer

Length: 732 bp
ATGAGATTTTTAAAACTAGGCCATATCCGGATCCAAATCACAACACATCTGTTCCTCAGTTGCTCAAACTTACTTTTCACTTCCTCCAGAGTCATGGCTGCCATCAAGGTCCACGGAAACGTTTTCTCGGCCTGTACAAGGCGAGTTTTAGCTGCTCTCTACGAGAAAGACGTCAAATTCGAGCTTGTTCCAATCGACTTGGGAGCTGGTGAACACAAAAAGGAGCCCTTCATTTCCCTCAATCCATTTGGTGAAGTTCCAGCTTTCGAACACGGAGATATTAAGCTCTTTGAATCAAGGGCAATTACACAATACATTGCCCACGAGTATGCTGACAAGGGAACCCCGCTGGTGACCCGAGACAAGAAGATGGCAATTATAGCATTGGGGTGTGAGGTGGAGGGCCAAAAGTTCGACCCGGCGGCCTCAAAACTGACCTTTGAGCTAGTTATAAAGCCTATGCTTAAAATGACCACAGATGCGGCGGTTGTGGAGGAATATGAAGCCAAGTTGGCTGTGGTTCTGGATGTATATGAGATTCGTCTGTCTCAGTCGAAATACGTGGCAGGCGAAAGCTTCACTTTGGCTGATCTTCACCACCTTCCCACCATACATTACTTGATGGGAACACGATCGAAGAAGCTGTTCGAGTCCCGCCCCCATGTTAAAGCATGGGTAGCGGATATCACAGCAAGGCCAGCTTGGAAAAAAGTCATTGCCCTGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

244

Amino Acids

27.34

Weight (kDa)

9.11

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AatII GACGTC 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 27
AciI CCGC 6 cut(s) 347, 422, 482, 485, 655, 680
AclI AACGTT 1 cut(s) 120
AclWI GGATC 2 cut(s) 25, 38
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 138
AgsI TTSAA 1 cut(s) 293
AluBI AGCT 9 cut(s) 152, 184, 206, 263, 286, 445, 576, 643, 701
AluI AGCT 9 cut(s) 152, 184, 206, 263, 286, 445, 576, 643, 701
Alw26I GTCTC 2 cut(s) 354, 552
AlwI GGATC 2 cut(s) 25, 38
Ama87I CYCGRG 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 27
AoxI GGCC 5 cut(s) 19, 129, 403, 423, 695
ApeKI GCWGC 2 cut(s) 98, 152
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
Asp700I GAANNNNTTC 1 cut(s) 644
AspS9I GGNCC 2 cut(s) 109, 403
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 4 cut(s) 221, 263, 364, 587
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 357
AvaII GGWCC 1 cut(s) 109
BamHI GGATCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 1 cut(s) 323
BbvI GCAGC 2 cut(s) 85, 139
BccI CCATC 3 cut(s) 110, 364, 616
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 2 cut(s) 354, 552
BfaI CTAG 2 cut(s) 17, 446
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 4 cut(s) 99, 153, 423, 483
BlsI GCNGC 4 cut(s) 100, 154, 424, 484
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 109, 403
BmiI GGNNCC 3 cut(s) 32, 225, 343
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 315, 714
Bse8I GATNNNNATC 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 138
BseMI GCAATG 2 cut(s) 315, 714
BseMII CTCAG 2 cut(s) 70, 563
BseXI GCAGC 2 cut(s) 85, 139
Bsh1285I CGRYCG 1 cut(s) 635
BshFI GGCC 5 cut(s) 21, 131, 405, 425, 697
BsiEI CGRYCG 1 cut(s) 635
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 2 cut(s) 28, 419
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 1 cut(s) 138
BsmAI GTCTC 2 cut(s) 354, 552
BsmFI GGGAC 1 cut(s) 637
BsnI GGCC 5 cut(s) 21, 131, 405, 425, 697
BsoBI CYCGRG 1 cut(s) 357
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 27
Bsp1407I TGTACA 1 cut(s) 134
Bsp143I GATC 3 cut(s) 30, 589, 632
BspACI CCGC 6 cut(s) 347, 422, 482, 485, 655, 680
BspANI GGCC 5 cut(s) 21, 131, 405, 425, 697
BspCNI CTCAG 2 cut(s) 69, 562
BspEI TCCGGA 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 32, 225, 343
BspPI GGATC 2 cut(s) 25, 38
BspT104I TTCGAA 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 315, 714
BsrGI TGTACA 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 30, 589, 632
BssNI GRCGYC 1 cut(s) 171
BssSI CACGAG 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 568, 699
BstDEI CTNAG 2 cut(s) 56, 549
BstDSI CCRYGG 1 cut(s) 112
BstEII GGTNACC 1 cut(s) 352
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 532
BstKTI GATC 3 cut(s) 33, 592, 635
BstMAI GTCTC 2 cut(s) 354, 552
BstMBI GATC 3 cut(s) 30, 589, 632
BstMCI CGRYCG 1 cut(s) 635
BstMWI GCNNNNNNNGC 4 cut(s) 380, 512, 677, 698
BstPI GGTNACC 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 85, 139
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 5 cut(s) 21, 131, 405, 425, 697
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 568, 699
Cfr13I GGNCC 2 cut(s) 109, 403
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 94, 662, 672
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 2 cut(s) 56, 549
DpnI GATC 3 cut(s) 32, 591, 634
DpnII GATC 3 cut(s) 30, 589, 632
DraI TTTAAA 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 357
Eco91I GGTNACC 1 cut(s) 352
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 352
EcoRV GATATC 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 228
FaeI CATG 3 cut(s) 97, 665, 675
FalI AAGNNNNNCTT 6 cut(s) 212, 244, 565, 597, 685, 717
FaqI GGGAC 1 cut(s) 637
FatI CATG 3 cut(s) 93, 661, 671
FauI CCCGC 2 cut(s) 354, 662
Fnu4HI GCNGC 4 cut(s) 99, 153, 423, 483
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 4 cut(s) 99, 153, 423, 483
FspBI CTAG 2 cut(s) 17, 446
GluI GCNGC 4 cut(s) 99, 153, 423, 483
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 5 cut(s) 21, 131, 405, 425, 697
HapII CCGG 2 cut(s) 28, 419
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 97, 665, 675
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 4 cut(s) 90, 293, 538, 650
HpaII CCGG 2 cut(s) 28, 419
HphI GGTGA 4 cut(s) 221, 263, 364, 587
Hpy166II GTNNAC 2 cut(s) 112, 212
Hpy188III TCNNGA 3 cut(s) 28, 87, 524
Hpy8I GTNNAC 2 cut(s) 112, 212
HpyAV CCTTC 2 cut(s) 238, 611
HpyCH4IV ACGT 3 cut(s) 120, 171, 561
HpyCH4V TGCA 1 cut(s) 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 512, 677, 698
HpyF3I CTNAG 2 cut(s) 56, 549
HpySE526I ACGT 3 cut(s) 120, 171, 561
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 97, 665, 675
Kpn2I TCCGGA 1 cut(s) 27
Kzo9I GATC 3 cut(s) 30, 589, 632
LmnI GCTCC 2 cut(s) 203, 223
Lsp1109I GCAGC 2 cut(s) 85, 139
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 17, 446
MaeII ACGT 3 cut(s) 120, 171, 561
MaeIII GTNAC 1 cut(s) 352
MalI GATC 3 cut(s) 32, 591, 634
MboI GATC 3 cut(s) 30, 589, 632
MboII GAAGA 3 cut(s) 379, 584, 649
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 3 cut(s) 176, 303, 375
MlyI GAGTC 2 cut(s) 99, 659
MnlI CCTC 7 cut(s) 65, 94, 248, 388, 394, 436, 487
MroI TCCGGA 1 cut(s) 27
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 4 cut(s) 11, 282, 465, 666
MslI CAYNNNNRTG 2 cut(s) 327, 388
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 28, 419
MspR9I CCNGG 1 cut(s) 419
MwoI GCNNNNNNNGC 4 cut(s) 380, 512, 677, 698
NciI CCSGG 1 cut(s) 419
NdeII GATC 3 cut(s) 30, 589, 632
NlaIII CATG 3 cut(s) 97, 665, 675
NlaIV GGNNCC 3 cut(s) 32, 225, 343
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 3 cut(s) 168, 177, 567
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 293, 538
PkrI GCNGC 4 cut(s) 100, 154, 424, 484
Ple19I CGATCG 1 cut(s) 635
PleI GAGTC 2 cut(s) 98, 658
PpsI GAGTC 2 cut(s) 98, 658
Ppu21I YACGTR 1 cut(s) 562
PsiI TTATAA 1 cut(s) 452
Psp1406I AACGTT 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 352
PspN4I GGNNCC 3 cut(s) 32, 225, 343
PspPI GGNCC 2 cut(s) 109, 403
PsuI RGATCY 1 cut(s) 30
PvuI CGATCG 1 cut(s) 635
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 2 cut(s) 327, 388
SaqAI TTAA 4 cut(s) 11, 282, 465, 666
SatI GCNGC 4 cut(s) 99, 153, 423, 483
Sau3AI GATC 3 cut(s) 30, 589, 632
Sau96I GGNCC 2 cut(s) 109, 403
SchI GAGTC 2 cut(s) 99, 659
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 469
SfuI TTCGAA 1 cut(s) 267
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 327, 388
Sse9I AATT 3 cut(s) 176, 303, 375
SsiI CCGC 6 cut(s) 347, 422, 482, 485, 655, 680
SspMI CTAG 2 cut(s) 17, 446
StyD4I CCNGG 1 cut(s) 417
TaiI ACGT 3 cut(s) 123, 174, 564
TaqI TCGA 7 cut(s) 180, 195, 267, 414, 554, 635, 648
TasI AATT 3 cut(s) 176, 303, 375
TatI WGTACW 1 cut(s) 134
TauI GCSGC 2 cut(s) 425, 485
TfiI GAWTC 2 cut(s) 293, 538
Tru1I TTAA 4 cut(s) 11, 282, 465, 666
Tru9I TTAA 4 cut(s) 11, 282, 465, 666
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 98, 152
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 220, 516
TspGWI ACGGA 2 cut(s) 129, 288
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 17, 446
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.