MD03G1282900.v1.1
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003136
Physical Location & Seq
Reverse (-)
36322193 .. 36324159
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1282900.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGCTCCAGTCAAGGTCCACGGAAACGTTTTGTCGGTCTGTACAAGGCGAGTGATTGCTGCTCTCTACGAGAAAGACATCAAATTTGAGCTTGTTCCAATCGACTTGGGAACTGGTGAACACAAAAAGGAGCCCTTCATTTCCCTCAATCCATTTGGTGAAGTTCCAGCTTTCGAAGACGGAGATCTTAAGCTCTTTGAATCAAGGGCAATTACACAATACATTGTCCACGAGTATGCTGACAAGGGAACCCCGCTGGTGTTCCAAGACTCTAAGAAGATGGCAATGATAGCAGTAGGGTGTGAGGTGGAGGGCCAAAAGTTCGACCCGGCGGCCTCAAAACTGACCTTTGAGCAAGTTATAAAGCCTATGCTTAAAATGCCCACAGATGCGGCAGTTGTGGAGGAATATGAAGCCAAGTTGGCTGTGGTTCTGGATGTGTATGAGATTCGTCTGGCTCAGTCGAAATACTTGGCAGGCGAAAGATTCACTTTGGCTGATCTTCACCACATTCCCTCCATACATTACTTGATGGGAACACAATCGAAGAAGCTGTTCGTGTCCCGCCCCCATGTTAGCGCATGGGTAGCTGATATCACAGCAAGGCCAGCTTGGAAGAAAGTCATTGCCCTGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

214

Amino Acids

23.85

Weight (kDa)

7.06

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 3 - 76 1.7e-18 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 11 - 81 5e-13 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 75 6.7e-11 Glutathione S-transferase, N-terminal domain
GST_C PF00043 124 - 203 1.8e-15 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 136 - 206 3.4e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AciI CCGC 4 cut(s) 254, 332, 392, 565
AclI AACGTT 1 cut(s) 27
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 1 cut(s) 43
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 200
AluBI AGCT 6 cut(s) 91, 170, 193, 553, 590, 611
AluI AGCT 6 cut(s) 91, 170, 193, 553, 590, 611
AoxI GGCC 3 cut(s) 313, 333, 605
ApeKI GCWGC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 16, 313
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 128, 170, 497
AsuII TTCGAA 1 cut(s) 174
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 46
BccI CCATC 2 cut(s) 274, 526
BcnI CCSGG 1 cut(s) 329
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 422
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 60, 333, 393
BlsI GCNGC 3 cut(s) 61, 334, 394
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 313
BmiI GGNNCC 3 cut(s) 6, 132, 250
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 379
BpiI GAAGAC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bse1I ACTGG 2 cut(s) 8, 118
Bse3DI GCAATG 2 cut(s) 291, 624
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 1 cut(s) 442
BseMI GCAATG 2 cut(s) 291, 624
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 2 cut(s) 8, 118
BseXI GCAGC 1 cut(s) 46
BshFI GGCC 3 cut(s) 315, 335, 607
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 1 cut(s) 547
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 3 cut(s) 315, 335, 607
Bsp119I TTCGAA 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 2 cut(s) 184, 499
BspACI CCGC 4 cut(s) 254, 332, 392, 565
BspANI GGCC 3 cut(s) 315, 335, 607
BspCNI CTCAG 1 cut(s) 472
BspLI GGNNCC 3 cut(s) 6, 132, 250
BspT104I TTCGAA 1 cut(s) 174
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 291, 624
BsrGI TGTACA 1 cut(s) 41
BsrI ACTGG 2 cut(s) 8, 118
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 2 cut(s) 184, 499
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 41
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 478, 609
BstDEI CTNAG 2 cut(s) 273, 459
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 2 cut(s) 187, 502
BstMBI GATC 2 cut(s) 184, 499
BstMWI GCNNNNNNNGC 5 cut(s) 290, 379, 422, 587, 608
BstSCI CCNGG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 46
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 3 cut(s) 315, 335, 607
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 478, 609
CfoI GCGC 1 cut(s) 581
Cfr13I GGNCC 2 cut(s) 16, 313
Csp6I GTAC 1 cut(s) 42
CviAII CATG 2 cut(s) 572, 582
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 2 cut(s) 273, 459
DpnI GATC 2 cut(s) 186, 501
DpnII GATC 2 cut(s) 184, 499
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 16
EcoRV GATATC 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 2 cut(s) 575, 585
FaiI YATR 8 cut(s) 237, 362, 371, 411, 444, 521, 573, 583
FalI AAGNNNNNCTT 6 cut(s) 119, 151, 475, 507, 595, 627
FaqI GGGAC 1 cut(s) 547
FatI CATG 2 cut(s) 571, 581
FauI CCCGC 2 cut(s) 261, 572
Fnu4HI GCNGC 3 cut(s) 60, 333, 393
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 3 cut(s) 60, 333, 393
GlaI GCGC 1 cut(s) 580
GluI GCNGC 3 cut(s) 60, 333, 393
HaeIII GGCC 3 cut(s) 315, 335, 607
HapII CCGG 1 cut(s) 329
HhaI GCGC 1 cut(s) 581
Hin1II CATG 2 cut(s) 575, 585
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HinfI GANTC 4 cut(s) 200, 269, 448, 486
HpaII CCGG 1 cut(s) 329
HphI GGTGA 3 cut(s) 128, 170, 497
Hpy166II GTNNAC 3 cut(s) 19, 119, 229
Hpy188III TCNNGA 1 cut(s) 434
Hpy8I GTNNAC 3 cut(s) 19, 119, 229
HpyAV CCTTC 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 379, 422, 587, 608
HpyF3I CTNAG 2 cut(s) 273, 459
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 575, 585
HspAI GCGC 1 cut(s) 579
Kzo9I GATC 2 cut(s) 184, 499
LmnI GCTCC 2 cut(s) 10, 130
LpnPI CCDG 9 cut(s) 21, 99, 180, 242, 342, 419, 440, 462, 621
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 1 cut(s) 379
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 186, 501
MboI GATC 2 cut(s) 184, 499
MboII GAAGA 5 cut(s) 188, 289, 494, 559, 628
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 83, 210
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 6 cut(s) 155, 298, 304, 346, 397, 526
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 2 cut(s) 189, 375
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 256
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 329
MwoI GCNNNNNNNGC 5 cut(s) 290, 379, 422, 587, 608
NciI CCSGG 1 cut(s) 329
NdeII GATC 2 cut(s) 184, 499
NlaIII CATG 2 cut(s) 575, 585
NlaIV GGNNCC 3 cut(s) 6, 132, 250
NspV TTCGAA 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 3 cut(s) 200, 448, 486
PkrI GCNGC 3 cut(s) 61, 334, 394
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PsiI TTATAA 1 cut(s) 362
Psp1406I AACGTT 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 6, 132, 250
PspPI GGNCC 2 cut(s) 16, 313
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 2 cut(s) 189, 375
SatI GCNGC 3 cut(s) 60, 333, 393
Sau3AI GATC 2 cut(s) 184, 499
Sau96I GGNCC 2 cut(s) 16, 313
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 2 cut(s) 83, 210
SsiI CCGC 4 cut(s) 254, 332, 392, 565
StyD4I CCNGG 1 cut(s) 327
TaiI ACGT 1 cut(s) 30
TaqI TCGA 5 cut(s) 102, 174, 324, 464, 545
TaqII GACCGA 1 cut(s) 25
TasI AATT 2 cut(s) 83, 210
TatI WGTACW 1 cut(s) 41
TauI GCSGC 2 cut(s) 335, 395
TfiI GAWTC 3 cut(s) 200, 448, 486
Tru1I TTAA 2 cut(s) 189, 375
Tru9I TTAA 2 cut(s) 189, 375
TseI GCWGC 1 cut(s) 59
TspDTI ATGAA 2 cut(s) 127, 426
TspGWI ACGGA 2 cut(s) 36, 195
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.