Rorug06G0234500
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003136
Physical Location & Seq
Reverse (-)
37681032 .. 37694716
13685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0234500.1

Sequence Viewer

Length: 7800 bp
ATGCGTATCGTCAGTTTTTTTGTGGATTCCATGCTCGTTGTTCAAGATCTTTTGTGTGTCGCATCAGTCTCTGCAGACATATCAAGTTTTACAGAATCAACTAGAGTGACTTTAAAGTGTTTCTGCGAGTCCTTCAATGCAATTTTTGATGATCCTTCACATCTTGGTGATCTCCCACATAGTATCAAACCTGTTGATGGCACTGGGATCTTGATTAACCTTACAGGAAAAGCAAGGTGGCAACCTTTCGCAACTTGGATGATTAAGCTTCTTGGAAAATGCTTAACTGAAGGAACTCTATATGTGGATGGACTCATCAATATGCCATTTGTTATGGCTGCATGGTCCCTTTTTTGTTATGGGGATGCTGATTTGCACATGGCATTTTTTGAATTTGCACGCATCGTTGGATCTGCGATAAAATGTGACGTCATTCCTTATCATAACATAATCCAGTCGATATCATCTATATTAAGCGAGGACAAAGAGGGGACTCCTGCATTTAGAAACACGGTTTATGATTCGTCCATGGGTGGTTGCCTAGAGGCATTGCACTCTAATTGCCCTGAGGGTATAGTCAAGCTAACAGCTGCACATTTTGTCAATGTATTTCCTCATTCAATGAGGAGAACCCGAAGCCAGGAGCTTAAGGTTGCATTGTGCAACACATACATACGGATTGCGAAGATTTGCCCCCCTCATATCTGGAGGCCAGAAATTCTCCTATATGTACTTTCATGTCCGGATCCCTGCTTTTCATTGATAGATTGCTTACATGTAGCTCTCTCTATACTTGGTCCTGATCTTGTTGGAGGAAGAATTACAGATAATTCCTGCCAGAGTTTATCCACATTAAGTGATAAATCAATTGAAAACTCACAGGTTGGAGAAAAAAGACCTATTGAGGATGTGGATTCTTTCAACACTAAACGCCAAAAGGTTGATGAAAAAATCATCAATCCTGATTCTAATATTCAGTTGGAATGGCAGTGTGCTCATATAGTTCCTTGTGAGACAGAAGACTATGCAAATCAGATGCATAAATCACTCCTTTCTTTTGCTGAATGTTTAAAATCTCCTGGTGTCTGTCCTAATTCCTTAAGACCTGATCTCGCGTTAATGGCTCTTAGCATTCTTTGCATTGCGTTCTGTAGATATCCTGAGACCAATCTGTCATTATGCATCTTTCATCAGATGTATACATGGATACCTTGGATATGCCAACAGGCAAAGCAAGGAAGCACAGTTGAACTTGATATTTCCATCTACCTAGAAGGAATTCACTGTATGTTGCGGTTGCAAAATTGCTTAATCATGAAGTGTATGGACAGTACGCTTGTCGATGCTGATCTTATACATGTCCTTCTAAAGCTTCCATGGACCCATTCTCATGTAGTTACTGACCGCCATCAATTGTGGAAAACAAAATGCATCTCAGTTCAAGTTGCATGCAAACTTGGTCATAGCACAAGAATTGAAACTCAACTTGAAGTATTGGATTTGAGTCTCAGTGATGAATGTGACGAAGTTATAATTGAAGCTATAACTTCTATGCCCGTGATTGTCATGTGGTCTGGCCTCAGTGGACTTTCTCACATATTCAGAAGGCTTGAGCTCTTGGGGCGACAGAGAAGTGAGAAGGTTAAGAATATCATTCCCTTTTCTCTTGGTTTTTTGTCATGTCTTTACGGATCTCATAGCAATGGAGATGACATAAATAGAAGTCAGTGTAAACTATTTTTAAAAATGAAAAGTGAGAGACACAGTCAAACAGTGGATTATTTACTGCAAGGATTCTGGTGTTCAAAGTGTGACCGAAGTGTTCAACACAATACTGAGTCATATTCAAATATCATAAGTCTACCTGACAAGCCCAGAGAATTGGACTGTAATTTTCTCCATCTTCAGTCTATATATTTTCAGCTTCTTAATGATGAGTCATCTGAAGAGATTCAAGTGGCTTGTGTGAAAAATATCCGCCGAGTACTTGTACATGGGGGCATAGAGAATCTGTTGGAGTCAAGATTTGAATGGATTAAATGTGTTAAATATCTACTCATTAATACCAAGAAGGCTATAAGAGAAGCATTCTGCACTCAGATTAGTTCATTCGTTGAGGATTCAATTTTGAGTTGCTTATTTCCTGATGAGGGCATGTCAAGTAATGGTAGAGAAGAAGGGTTTTTGAACATAATCAACAGCTTCTTTTCAGAAACTAAAGACCTCCAAATCATTGAGACTCTTTTGGAATCAACAGCTGAAATAATGATTGCAGTTGATATTCAGAGTCAATTATTCTTGTTTTCTCTTCTTTTGTTGGTTGGTCAACTTGATAATCCACACACGACAGTGAGAATGATTGCAGCGAAGTTGATACACAAAGCTTGCTGTTTTCATCGTAAAGGAGGTTTCCAAGTAATCCTTCTGAAAGTTGTTCATGTTCGAAATGAGCTATTTGACTATCTGTCTTCGAGGCTCTCTAGCCGTCCGACTATGGTCCAAGAGTTTGCAGAATCAGTTCTTGGTATTGAAACAGAAGAACTTGTCAAGAAAATGGTTCCCGTAGTTCTTCCGAAGCTTGTAGTGTCTCAGCAGGATGATGATCAAGCAGTTGACACCTTATATGAACTGGCCAAGTGGTTAAACACCGATGTTGCAGGTCTTATAGTTCTATGGGTGCCAAAAGTGCTAGCTTTTGCTCTCCATCAACCAGATGTGCGAGAATTAGATGCTGCTGTACAGTTTTACCATACACAAATCCATTCTAACAAAAGAGAAATCTTTGCTGCTGCATTAAATAATCTTTTAGATGAACTCATATGCTTTCTGGATGGAGGTGATTCAGATGAGATAAGCGGAAGGTTAGCAAGAGTACCTGAGACGATAAAAGAGGTTGCTAGAGTTTTGGATGAAGGTGAAGATCTACCAAACTTTTTGAGGAATCATTTTGCCCACCTTCTTAACAGTATCGATAGAAAGATGCTCCATGCAGAAGAATTTTCATTGCAGAAACAAGCCTTGCAACGAATTGAGATGCTAATTAAAATGATGGGTCGTCATCTTAGTACTTATGTGCCTAAACTAATGGTTCTTCTCATGCATGCTATTGATAAAGACATCCTCCAGAGTGAGGGTCTCTCAGTGTTGCATTTTTTCATTGTGCAGCTGGTGAAAGTATCACCGGCTAGCACTAAACATGTAATTTCACAAGTTTTTGCTGCCCTTATTCCCTTTCTTGAGAGAGATGAAGAAAGTCCAGTACATCTGGATAAAGTGGTAAAAATTTTAGAAGAACTTGTGCTAAATAACAGGGATATTCTAAAGCAGCATATTCGTGAGTTCCCTCCATTGCCCAGTATTCCAGCCTTAATAAAAGTGAACAAGGCTATACAAGAAGCCAGAGGATCAATGACTTTGAGGGATCGATTGCGGGATGTTGTTGATGGTCTGAATCATGACAGCTTAAATGTGAGATACATGGTTGTTTGTGAGTTGAAAAAGTTGCTGAACCTGGAACGGGAAGGGGTTACATCTCTAATAACTGCTGAAGGTGGTGCGGATATGGAAATTTTGAGCTCTTTAATCAGATCTCTACTTAAAGGATGTGCTGAAGAATCAAGGAGAGCAGTTGGACAGCGGCTAAAGTTGGTGTGTGCTGACTGCCTTGGGGCCCTGGGTGCAGTTGACCCTGCTAAAGTGAAGGCCTTTTCATGCCAACGCTTTAAAATTGAATGCTCTGATGATGACCTTATCTATGAGTTGATTCAGAAGCAACTTGCACGGGCCTTTAGAGCTGCACCTGACACTATAATTCAAGATTCAGCTGTAGTGGCCATACAGGAACTGCTAAAGATTGCAGGTTGTGAGGCCTCATTACATGAGAATGCTGCTGCTTCTATGTCGGCTCCAAAGGACAAAGAGCCTCTGACAGGTTCTGCTTCTGGAATGATGACTACTGATGGTACAAATGAGATGACTAGAAGGGGTCAGAGATTATGGGACCGGTTCTCCAAATATGTGAAAGAAATAATTGCCCCTTATTTGTCCTCTAGATTTCAACTTCCTAATGTTGCTGATCCTGTATTTGTTGGCCCAATTTACCGTCCATCTATGTCATTCAGAAGATGGATATTCTTCTGGATAAGAAAGCTGACTGCACATGCTACTGGTTCTCGTGCAAGAATTTTCAATGCTTGTCGAGGTATAGTGCGCCAGGATATGCAAACAGCAATTTATCTGCTTCCATATTTAGTTCTTAATGCTGTCTGTCATGGCACAGAGGAGGCACGCTATGGCATAACAGAAGAAATCCTATATGTTCTTGATGTTGCTGCAACTCATAACAGTGAGGATGCAGTGCATGGTGTCAATGGTCGGAAAAATGAAGTTTGTGTTCAAGCTGTGTTCATTCTCCTTGATAATCTTGGTCAATGGGTGGATGACGTTAGACAAGAACTATTCCGTTCTTTTCAATTATTAGCTTCTAAGCAACAAATATCCAAGACCAGGCATCAAACTCAGAATACTACCAATCAGGACCAACTTCGTCTACAGTGTGAACATGTTTCAAAACTCTTGTTTGCAGTTCCAAAGGAGACCCTTGCCAGGGCCTCCTTCAGGTGTCAGGCGTATGCAAGATCCTTCATGTATTTTGAGTTGTACGTGAGGGGAAAGTCAGGTTCTTTCAACCCTGCATCTGAGCAAAGTGGTTCATTTGATGATGCAGATGTTTCATATCTGATGGAAATATACAGCTGCCTGGATGAGCCTGATGGTCTTTCTGGATTTGCATTTTTACGTAAATCTTTGGAATTACAAGACCAGCTCTTGATAAACAAAAAAGCCGGGAACTGGGCAGAGGTTTTAACTTCTTGTGAACAAGCTTTGCAGATGGAACCAACATCAGTTCAAAAGCAGTCTGATGTGCTTAACTGTTTATTAAACATGTGTCACCTTCAGGCTATGGTGACTCATGTGGATGGTTTAATGTGTAGGATTCCCCAATACAAGAAAACATGGTGCATGCAAGGTGTGCAGGCAGCATGGAGACTTGGCAGGTGGGATTTGATGGATGAGTACCTTAGTGGAGCTGATCAAGAAGGTTTACTTTGTAGCAGTTCTGAAAGTAATGCGTCATTTGATATGGATGTTGCAAAAATTCTGCAGGCAATGATGAAGAAGGATCAGTTCTCAGTTGAAGAAAAAATTGCCCTCTCCAAGCAAGCTCTAATTGCTCCCCTTTCTGCTGCAGGCATGGATTCTTATGTGCGGGCTTACCCATTTGTCGTGAAACTTCACCTGCTACGAGAGCTTGAGGATTTTTATACTCTTCTTGGTGATGCTTCTTTCTTGGAGAAATCGTTTGCATTTGATGATCCTAAATTCTCAAAAGTGAGGGAGAACTGGGAAAACCGTCTCAGATTTACTCAGCCATCACTCTGGGCAAGAGAGCCACTTTTGGCTTTCCGAAGATTGGTTTTTGCTAACAGTGGTCTTGAGGCTCAAGTTGGGAACTGCTGGCTTCAATATGCAAAGCTCTGTCGCATGGCTGGTCATTATGAGACAGCAAATCAGGCTATTCTAGAAGCCCAAGCTACCAATGCACCTAACGTTCACATGGAGAAGGCTAAACTTCTCTGGAGCACCAGGCGATCTGATGGTGCAATCGCACAGTTGCAACAGTGTCTCTTGAACATGCCCGAAGGGATTGTAGGCTCCAATGCAATTGCATCAATTACCAGTCTTTCACTGGTTCCGCCAAACCTGCCCCCTTTAGTTAATAATACTCAAGCTTGGAATGAAAATCGAGATATTGCAAAGACACTTCTCCTCTATTCTAGGTGGATCCATTACACTGGGCAGAAGCAGAAAGAAGATGTCATAAGTCTATATAGCAGGGTGAGGGAACTGCAGCCCAAGTGGGAGAAAGGATATTTTTACATGGCTAAATATTATGAGGAAGTGCTAGCTGATGCCAGGAAGCGTCAGGAAGAAAATCTTGAACTGGGTCCTAGGAAATTACCATCAAATAGTGCTATTGTTGGTTCTTCAAATTTAAATACAGAGAAGGCTTGGTGGGCATATGTGCCTGACATACTGATATTCTATGCAAAAGGGCTTCATCGGGGCCACAAGAATCTCTTTCAGGCACTTCCAAGGTTGTTAACCCTATGGTTTGACTTTGGGACTATGTATGAAAGGAGTGGCACATCATCCAATAAGGATCTCAAAAATGTCCACATGATGGTGATGAGATGCATGCGAGGGTGTTTGAAGGACTTGCCAACATATCAGTGGCTAACAGTACTGCCACAGTTGGTTTCAAGAATCTGCCACCAGAATGAGGAAGTAGTTCGATTGGTCAAAAGCATCATCACATCAGTTCTCCGGCAATACCCACAACAAGCCCTGTGGATTATGGCAGCGGTTTCAAAATCCACTGTTCCATCCAGGAGGGATGCAGCTGCTGAAATTCTACAGGAGGCACGGAAAGGGTTCAGCCAAGGAACTGGTGGAAGCAATCTGTTTGTTCAGTTTGCCAGTCTGATTGATCATTTTATCAAGTTGTGCTTCCATGCTTGTCAGACAAAGGCAAAAACAATCAATGTCTCTACTGAATTTAGTCTCTTAAAGAGGATGATGCCGCTGGGAATTATCATGCCAATTCAGCAATCTCTAACTGTTAGCCTACCGACATACGATGGGAAGCTCACTGACTCACTTAAGTCTGATATCTTTTCTGCGACTGATCTTCCTACAATATCAGGAATACGTGATGAGGCTGAGATTCTTTCTTCCCTTCAGCGACCTAAGAAAATTGTTCTATTGGGCAGCGACGGCATTGAACGTCCGTTCCTCTGCAAACCCAAGGATGACCTCAGGAAAGATGCCCGGATGATGGAGTTCACAGCAATGATAAACCGTTTGTTGTCTAAATACCCAGCAAGCCGTCGGAGGAAGCTCTACATTCGCACCTTTGCAGTAATTCCTTTGACAGAGGATTGTGGCATGATAGAATGGGTGCCCCACACACGTGGACTTCGGCATATACTTCAAGACATATATATGAACTGCGGAAACTTTGGTATTGATACAAATTCAAAGATCAAGCGCTTATATGATCAATGCAAGGGTAAAATACCAGAAGATGAGATGTTGAAGGACAAAATTCTTCCAATGTTCCCTCCAGTTTTCCACAAGTGGTTCCTGACCACTTTTTCTGAGCCAGCTGCTTGGTTTAGGGCACGGATTGCTTATGCACACACTACTGCAGTTTGGTCCATGGTTGGACATATTGTGGGGCTTGGTGACCGACACGGGGAAAACATTCTTTTTGATTCTACAACAGGTGATTGTGTTCATGTTGATTTCAGTTGCTTATTCGATAAAGGCTTGCAGTTGGAGAAGCCTGAACTGGTGCCTTTCAGATTGACTCAGAATATGATTGATGGCTTGGGCATCACTGGTTATGAGGGAATCTTTTTGAGGATCTGCGAAATCACACTTTCAGTACTGAGGATGCATCGGGAGACATTGATGAGTGTTCTTGAGACTTTTGTCCATGATCCTCTTGTAGAATGGACAAAATCACACAAATCTAGTAGAGTAGAAGTTCAGAATCCACACGCACAGCTAGCCATCAGTAACATTGAGGCAAGATTGCAAGGAGTAGTTGTCGGTGTCGGAGCAGCACCATCTTTGCCCTTGGCTGTAGAAGGTCAGGCTCGTCGATTAATAGCTGAAGCAGTCTCCCACAAAAATCTCGGAAAGATGTATATCTGGTGGATGCCATGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

2599

Amino Acids

294.1

Weight (kDa)

6.66

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UME PF08064 978 - 1078 2.7e-16 UME (NUC010) domain
M-HEAT_ATR PF25030 1365 - 1610 3.9e-32 Serine/threonine-protein kinase ATR, M-HEAT region
FAT PF02259 1677 - 2056 3.3e-59 FAT domain
HEAT_ATR PF23593 2083 - 2182 6.1e-32 Serine/threonine-protein kinase ATR-like, HEAT repeats
PI3_PI4_kinase PF00454 2284 - 2528 3.2e-56 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2568 - 2599 9.7e-13 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1532
AarI CACCTGC 2 cut(s) 5046, 5307
AasI GACNNNNNNGTC 1 cut(s) 1171
AatII GACGTC 1 cut(s) 432
Acc36I ACCTGC 5 cut(s) 2648, 3848, 5046, 5307, 5772
AccB1I GGYRCC 3 cut(s) 2677, 7021, 7417
AccB7I CCANNNNNTGG 1 cut(s) 6244
AccI GTMKAC 3 cut(s) 1199, 1861, 4549
AccII CGCG 1 cut(s) 1115
AccIII TCCGGA 1 cut(s) 742
AclI AACGTT 1 cut(s) 5610
AcoI YGGCCR 2 cut(s) 2631, 3831
AcuI CTGAAG 9 cut(s) 309, 1889, 1965, 3567, 3630, 4600, 4940, 6785, 7761
AcvI CACGTG 1 cut(s) 7034
AcyI GRCGYC 1 cut(s) 429
AfeI AGCGCT 1 cut(s) 7112
AflII CTTAAG 3 cut(s) 647, 1099, 6722
AflIII ACRYGT 6 cut(s) 775, 1357, 3196, 4561, 4944, 7031
AgeI ACCGGT 1 cut(s) 4002
AhdI GACNNNNNGTC 1 cut(s) 2464
AjuI GAANNNNNNNTTGG 4 cut(s) 2406, 2438, 2505, 2537
AleI CACNNNNGTG 2 cut(s) 2348, 7032
AloI GAACNNNNNNTCC 2 cut(s) 6837, 6869
Alw21I GWGCWC 4 cut(s) 997, 1617, 3578, 5645
AlwNI CAGNNNCTG 4 cut(s) 71, 3844, 3935, 6467
Aor13HI TCCGGA 1 cut(s) 742
Aor51HI AGCGCT 1 cut(s) 7112
ApaI GGGCCC 1 cut(s) 3673
AseI ATTAAT 2 cut(s) 2061, 7733
AsiGI ACCGGT 1 cut(s) 4002
Asp700I GAANNNNTTC 6 cut(s) 1278, 1950, 2517, 6351, 6494, 7326
AspA2I CCTAGG 1 cut(s) 6011
AspLEI GCGC 2 cut(s) 4212, 7113
AsuC2I CCSGG 2 cut(s) 4846, 6892
AsuII TTCGAA 1 cut(s) 2443
AsuNHI GCTAGC 4 cut(s) 2689, 3185, 5965, 7633
AvaII GGWCC 8 cut(s) 345, 797, 1380, 2497, 4000, 4537, 6008, 7278
AvrII CCTAGG 1 cut(s) 6011
AxyI CCTNAGG 2 cut(s) 567, 6878
BaeGI GKGCMC 3 cut(s) 3673, 7026, 7246
BalI TGGCCA 2 cut(s) 2633, 3833
BamHI GGATCC 2 cut(s) 745, 5844
BanI GGYRCC 3 cut(s) 2677, 7021, 7417
BanII GRGCYC 3 cut(s) 1617, 3578, 3673
BauI CACGAG 1 cut(s) 4173
BbrPI CACGTG 1 cut(s) 7034
BbsI GAAGAC 2 cut(s) 1026, 2460
Bbv12I GWGCWC 4 cut(s) 997, 1617, 3578, 5645
BceAI ACGGC 3 cut(s) 2469, 6853, 6933
BcgI CGANNNNNNTGC 2 cut(s) 3314, 3348
BciVI GTATCC 1 cut(s) 1200
BclI TGATCA 4 cut(s) 2602, 5092, 6550, 7120
BcnI CCSGG 2 cut(s) 4846, 6892
BfoI RGCGCY 1 cut(s) 7114
BfrI CTTAAG 3 cut(s) 647, 1099, 6722
BfuAI ACCTGC 5 cut(s) 2648, 3848, 5046, 5307, 5772
BfuI GTATCC 1 cut(s) 1200
BglII AGATCT 3 cut(s) 46, 2920, 3587
BlnI CCTAGG 1 cut(s) 6011
BmcAI AGTACT 4 cut(s) 1986, 3067, 6306, 7512
Bme18I GGWCC 8 cut(s) 345, 797, 1380, 2497, 4000, 4537, 6008, 7278
BmeRI GACNNNNNGTC 1 cut(s) 2464
BmrI ACTGGG 6 cut(s) 213, 3348, 4861, 5413, 5865, 6014
BmtI GCTAGC 4 cut(s) 2693, 3189, 5969, 7637
BmuI ACTGGG 6 cut(s) 213, 3348, 4861, 5413, 5865, 6014
BoxI GACNNNNGTC 2 cut(s) 2495, 7556
BpiI GAAGAC 2 cut(s) 1026, 2460
BplI GAGNNNNNCTC 2 cut(s) 3122, 3154
BpmI CTGGAG 4 cut(s) 727, 3107, 5659, 7170
Bpu14I TTCGAA 1 cut(s) 2443
BpuEI CTTGAG 7 cut(s) 1631, 3257, 5333, 5487, 5516, 5772, 7568
BpuMI CCSGG 2 cut(s) 4846, 6892
Bsa29I ATCGAT 2 cut(s) 2970, 3424
BsaAI YACGTR 4 cut(s) 4663, 4799, 6773, 7034
BsaBI GATNNNNATC 3 cut(s) 1347, 2601, 7775
BsaHI GRCGYC 1 cut(s) 429
BsaI GGTCTC 3 cut(s) 1157, 3140, 4589
BsaWI WCCGGW 2 cut(s) 742, 4002
BsaXI ACNNNNNCTCC 6 cut(s) 3992, 4022, 5345, 5375, 7659, 7689
Bse118I RCCGGY 2 cut(s) 3181, 4002
Bse21I CCTNAGG 2 cut(s) 567, 6878
Bse3DI GCAATG 7 cut(s) 548, 1140, 1708, 3002, 3347, 5175, 6918
Bse8I GATNNNNATC 3 cut(s) 1347, 2601, 7775
BseAI TCCGGA 1 cut(s) 742
BseCI ATCGAT 2 cut(s) 2970, 3424
BseJI GATNNNNATC 3 cut(s) 1347, 2601, 7775
BseMI GCAATG 7 cut(s) 548, 1140, 1708, 3002, 3347, 5175, 6918
BseRI GAGGAG 3 cut(s) 640, 4295, 5819
BseSI GKGCMC 3 cut(s) 3673, 7026, 7246
BseYI CCCAGC 2 cut(s) 6646, 6940
BsgI GTGCAG 7 cut(s) 576, 2077, 3182, 3699, 3780, 4140, 5054
Bsh1236I CGCG 1 cut(s) 1115
BshNI GGYRCC 3 cut(s) 2677, 7021, 7417
BshTI ACCGGT 1 cut(s) 4002
BshVI ATCGAT 2 cut(s) 2970, 3424
BsiHKAI GWGCWC 4 cut(s) 997, 1617, 3578, 5645
BsiSI CCGG 6 cut(s) 743, 3182, 4003, 4845, 6388, 6892
BslFI GGGAC 4 cut(s) 331, 505, 4013, 6199
BsmBI CGTCTC 2 cut(s) 2873, 5420
BsmFI GGGAC 4 cut(s) 331, 505, 4013, 6199
BsmI GAATGC 4 cut(s) 1131, 2087, 3737, 3889
Bso31I GGTCTC 3 cut(s) 1157, 3140, 4589
Bsp119I TTCGAA 1 cut(s) 2443
Bsp120I GGGCCC 1 cut(s) 3669
Bsp1286I GDGCHC 7 cut(s) 997, 1617, 3578, 3673, 5645, 7026, 7246
Bsp13I TCCGGA 1 cut(s) 742
Bsp1407I TGTACA 2 cut(s) 1990, 2737
Bsp19I CCATGG 4 cut(s) 528, 1376, 7281, 7790
BspDI ATCGAT 2 cut(s) 2970, 3424
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 1 cut(s) 1115
BspHI TCATGA 2 cut(s) 1314, 3454
BspMAI CTGCAG 5 cut(s) 76, 5166, 5251, 5913, 7273
BspMI ACCTGC 5 cut(s) 2648, 3848, 5046, 5307, 5772
BspOI GCTAGC 4 cut(s) 2693, 3189, 5969, 7637
BspT104I TTCGAA 1 cut(s) 2443
BspT107I GGYRCC 3 cut(s) 2677, 7021, 7417
BspTI CTTAAG 3 cut(s) 647, 1099, 6722
BspTNI GGTCTC 3 cut(s) 1157, 3140, 4589
BsrDI GCAATG 7 cut(s) 548, 1140, 1708, 3002, 3347, 5175, 6918
BsrFI RCCGGY 2 cut(s) 3181, 4002
BsrGI TGTACA 2 cut(s) 1990, 2737
BssAI RCCGGY 2 cut(s) 3181, 4002
BssNAI GTATAC 1 cut(s) 1200
BssNI GRCGYC 1 cut(s) 429
BssSI CACGAG 1 cut(s) 4173
Bst1107I GTATAC 1 cut(s) 1200
Bst2BI CACGAG 1 cut(s) 4173
Bst6I CTCTTC 3 cut(s) 1941, 2313, 5334
BstACI GRCGYC 1 cut(s) 429
BstAFI CTTAAG 3 cut(s) 647, 1099, 6722
BstAPI GCANNNNNTGC 3 cut(s) 1137, 5032, 7250
BstAUI TGTACA 2 cut(s) 1990, 2737
BstBAI YACGTR 4 cut(s) 4663, 4799, 6773, 7034
BstBI TTCGAA 1 cut(s) 2443
BstDSI CCRYGG 4 cut(s) 528, 1376, 7281, 7790
BstEII GGTNACC 1 cut(s) 7307
BstENI CCTNNNNNAGG 2 cut(s) 3927, 4615
BstFNI CGCG 1 cut(s) 1115
BstH2I RGCGCY 1 cut(s) 7114
BstHHI GCGC 2 cut(s) 4212, 7113
BstPAI GACNNNNGTC 2 cut(s) 2495, 7556
BstPI GGTNACC 1 cut(s) 7307
BstSLI GKGCMC 3 cut(s) 3673, 7026, 7246
BstSNI TACGTA 1 cut(s) 4799
BstUI CGCG 1 cut(s) 1115
BstV2I GAAGAC 2 cut(s) 1026, 2460
BstXI CCANNNNNNTGG 6 cut(s) 1882, 5439, 5855, 6509, 7034, 7233
BstZ17I GTATAC 1 cut(s) 1200
Bsu15I ATCGAT 2 cut(s) 2970, 3424
Bsu36I CCTNAGG 2 cut(s) 567, 6878
BsuI GTATCC 1 cut(s) 1200
BsuTUI ATCGAT 2 cut(s) 2970, 3424
BtgI CCRYGG 4 cut(s) 528, 1376, 7281, 7790
BtsI GCAGTG 2 cut(s) 995, 4362
BveI ACCTGC 5 cut(s) 2648, 3848, 5046, 5307, 5772
CaiI CAGNNNCTG 4 cut(s) 71, 3844, 3935, 6467
CciI TCATGA 2 cut(s) 1314, 3454
CfoI GCGC 2 cut(s) 4212, 7113
Cfr10I RCCGGY 2 cut(s) 3181, 4002
ClaI ATCGAT 2 cut(s) 2970, 3424
CseI GACGC 2 cut(s) 5121, 5972
CspAI ACCGGT 1 cut(s) 4002
CspCI CAANNNNNGTGG 2 cut(s) 6392, 6427
DraI TTTAAA 5 cut(s) 114, 1071, 1743, 3724, 6057
DrdI GACNNNNNNGTC 1 cut(s) 1171
DriI GACNNNNNGTC 1 cut(s) 2464
DseDI GACNNNNNNGTC 1 cut(s) 1171
EaeI YGGCCR 2 cut(s) 2631, 3831
Eam1104I CTCTTC 3 cut(s) 1941, 2313, 5334
Eam1105I GACNNNNNGTC 1 cut(s) 2464
EarI CTCTTC 3 cut(s) 1941, 2313, 5334
EciI GGCGGA 2 cut(s) 1967, 5745
Ecl136II GAGCTC 2 cut(s) 1615, 3576
Eco105I TACGTA 1 cut(s) 4799
Eco147I AGGCCT 2 cut(s) 3704, 3869
Eco24I GRGCYC 3 cut(s) 1617, 3578, 3673
Eco31I GGTCTC 3 cut(s) 1157, 3140, 4589
Eco32I GATATC 3 cut(s) 462, 1157, 6733
Eco47I GGWCC 8 cut(s) 345, 797, 1380, 2497, 4000, 4537, 6008, 7278
Eco47III AGCGCT 1 cut(s) 7112
Eco53kI GAGCTC 2 cut(s) 1615, 3576
Eco57I CTGAAG 9 cut(s) 309, 1889, 1965, 3567, 3630, 4600, 4940, 6785, 7761
Eco72I CACGTG 1 cut(s) 7034
Eco81I CCTNAGG 2 cut(s) 567, 6878
Eco91I GGTNACC 1 cut(s) 7307
EcoICRI GAGCTC 2 cut(s) 1615, 3576
EcoNI CCTNNNNNAGG 2 cut(s) 3927, 4615
EcoO109I RGGNCCY 4 cut(s) 3669, 3670, 4608, 6008
EcoO65I GGTNACC 1 cut(s) 7307
EcoRI GAATTC 1 cut(s) 1278
EcoRV GATATC 3 cut(s) 462, 1157, 6733
EcoT22I ATGCAT 6 cut(s) 1041, 1184, 1433, 3102, 6260, 7524
EcoT38I GRGCYC 3 cut(s) 1617, 3578, 3673
Esp3I CGTCTC 2 cut(s) 2873, 5420
FaqI GGGAC 4 cut(s) 331, 505, 4013, 6199
FauI CCCGC 2 cut(s) 3423, 5262
FauNDI CATATG 2 cut(s) 2819, 6082
FbaI TGATCA 4 cut(s) 2602, 5092, 6550, 7120
FblI GTMKAC 3 cut(s) 1199, 1861, 4549
FriOI GRGCYC 3 cut(s) 1617, 3578, 3673
GlaI GCGC 2 cut(s) 4211, 7112
GsaI CCCAGC 2 cut(s) 6650, 6944
GsuI CTGGAG 4 cut(s) 727, 3107, 5659, 7170
HaeII RGCGCY 1 cut(s) 7114
HapII CCGG 6 cut(s) 743, 3182, 4003, 4845, 6388, 6892
HgaI GACGC 2 cut(s) 5121, 5972
HhaI GCGC 2 cut(s) 4212, 7113
Hin1I GRCGYC 1 cut(s) 429
Hin6I GCGC 2 cut(s) 4210, 7111
HinP1I GCGC 2 cut(s) 4210, 7111
HincII GTYRAC 4 cut(s) 2327, 2614, 3685, 6165
HindII GTYRAC 4 cut(s) 2327, 2614, 3685, 6165
HindIII AAGCTT 6 cut(s) 266, 1370, 2382, 2576, 4881, 5790
HpaI GTTAAC 1 cut(s) 6165
HpaII CCGG 6 cut(s) 743, 3182, 4003, 4845, 6388, 6892
Hpy99I CGWCG 3 cut(s) 6839, 6954, 7731
HpyCH4IV ACGT 8 cut(s) 429, 4443, 4662, 4798, 5610, 6772, 6847, 7033
HpySE526I ACGT 8 cut(s) 429, 4443, 4662, 4798, 5610, 6772, 6847, 7033
Hsp92I GRCGYC 1 cut(s) 429
HspAI GCGC 2 cut(s) 4210, 7111
Kpn2I TCCGGA 1 cut(s) 742
Ksp22I TGATCA 4 cut(s) 2602, 5092, 6550, 7120
KspAI GTTAAC 1 cut(s) 6165
LmnI GCTCC 8 cut(s) 643, 2988, 3910, 5087, 5239, 5640, 5720, 7685
MaeII ACGT 8 cut(s) 429, 4443, 4662, 4798, 5610, 6772, 6847, 7033
MfeI CAATTG 3 cut(s) 867, 1412, 5724
MhlI GDGCHC 7 cut(s) 997, 1617, 3578, 3673, 5645, 7026, 7246
MlsI TGGCCA 2 cut(s) 2633, 3833
MluNI TGGCCA 2 cut(s) 2633, 3833
Mox20I TGGCCA 2 cut(s) 2633, 3833
Mph1103I ATGCAT 6 cut(s) 1041, 1184, 1433, 3102, 6260, 7524
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 6 cut(s) 1278, 1950, 2517, 6351, 6494, 7326
MscI TGGCCA 2 cut(s) 2633, 3833
Msp20I TGGCCA 2 cut(s) 2633, 3833
MspCI CTTAAG 3 cut(s) 647, 1099, 6722
MspI CCGG 6 cut(s) 743, 3182, 4003, 4845, 6388, 6892
MunI CAATTG 3 cut(s) 867, 1412, 5724
Mva1269I GAATGC 4 cut(s) 1131, 2087, 3737, 3889
MvnI CGCG 1 cut(s) 1115
NciI CCSGG 2 cut(s) 4846, 6892
NcoI CCATGG 4 cut(s) 528, 1376, 7281, 7790
NdeI CATATG 2 cut(s) 2819, 6082
NheI GCTAGC 4 cut(s) 2689, 3185, 5965, 7633
NmeAIII GCCGAG 1 cut(s) 2006
NmuCI GTSAC 7 cut(s) 106, 425, 1520, 1811, 4949, 4966, 7307
NsiI ATGCAT 6 cut(s) 1041, 1184, 1433, 3102, 6260, 7524
NspV TTCGAA 1 cut(s) 2443
OliI CACNNNNGTG 2 cut(s) 2348, 7032
PaeI GCATGC 4 cut(s) 1452, 3104, 5026, 6262
PagI TCATGA 2 cut(s) 1314, 3454
PaqCI CACCTGC 2 cut(s) 5046, 5307
PasI CCCWGGG 1 cut(s) 3673
PceI AGGCCT 2 cut(s) 3704, 3869
PciI ACATGT 5 cut(s) 775, 1357, 3196, 4561, 4944
PctI GAATGC 4 cut(s) 1131, 2087, 3737, 3889
PdmI GAANNNNTTC 6 cut(s) 1278, 1950, 2517, 6351, 6494, 7326
PflFI GACNNNGTC 1 cut(s) 1764
PflMI CCANNNNNTGG 1 cut(s) 6244
PfoI TCCNGGA 1 cut(s) 6449
PinAI ACCGGT 1 cut(s) 4002
PmaCI CACGTG 1 cut(s) 7034
PmlI CACGTG 1 cut(s) 7034
Ppu21I YACGTR 4 cut(s) 4663, 4799, 6773, 7034
PpuMI RGGWCCY 1 cut(s) 6008
PscI ACATGT 5 cut(s) 775, 1357, 3196, 4561, 4944
PshAI GACNNNNGTC 2 cut(s) 2495, 7556
PshBI ATTAAT 2 cut(s) 2061, 7733
PsiI TTATAA 1 cut(s) 1532
Psp124BI GAGCTC 2 cut(s) 1617, 3578
Psp1406I AACGTT 1 cut(s) 5610
Psp5II RGGWCCY 1 cut(s) 6008
PspCI CACGTG 1 cut(s) 7034
PspEI GGTNACC 1 cut(s) 7307
PspFI CCCAGC 2 cut(s) 6646, 6940
PspOMI GGGCCC 1 cut(s) 3669
PspPPI RGGWCCY 1 cut(s) 6008
PstI CTGCAG 5 cut(s) 76, 5166, 5251, 5913, 7273
PstNI CAGNNNCTG 4 cut(s) 71, 3844, 3935, 6467
PsyI GACNNNGTC 1 cut(s) 1764
PvuII CAGCTG 7 cut(s) 590, 2258, 3166, 3824, 4755, 6464, 7229
SacI GAGCTC 2 cut(s) 1617, 3578
ScaI AGTACT 4 cut(s) 1986, 3067, 6306, 7512
SduI GDGCHC 7 cut(s) 997, 1617, 3578, 3673, 5645, 7026, 7246
SfuI TTCGAA 1 cut(s) 2443
SinI GGWCC 8 cut(s) 345, 797, 1380, 2497, 4000, 4537, 6008, 7278
SmiI ATTTAAAT 1 cut(s) 6057
SnaBI TACGTA 1 cut(s) 4799
SphI GCATGC 4 cut(s) 1452, 3104, 5026, 6262
SseBI AGGCCT 2 cut(s) 3704, 3869
SspI AATATT 2 cut(s) 973, 5951
SstI GAGCTC 2 cut(s) 1617, 3578
StuI AGGCCT 2 cut(s) 3704, 3869
SwaI ATTTAAAT 1 cut(s) 6057
TaiI ACGT 8 cut(s) 432, 4446, 4665, 4801, 5613, 6775, 6850, 7036
TaqII GACCGA 2 cut(s) 1830, 7326
TatI WGTACW 8 cut(s) 730, 1984, 1990, 2737, 3065, 3259, 6304, 7510
TauI GCSGC 2 cut(s) 3640, 6646
TseFI GTSAC 7 cut(s) 106, 425, 1520, 1811, 4949, 4966, 7307
Tsp45I GTSAC 7 cut(s) 106, 425, 1520, 1811, 4949, 4966, 7307
TspGWI ACGGA 6 cut(s) 691, 1704, 4451, 6502, 6840, 7261
Tth111I GACNNNGTC 1 cut(s) 1764
Van91I CCANNNNNTGG 1 cut(s) 6244
Vha464I CTTAAG 3 cut(s) 647, 1099, 6722
VpaK11BI GGWCC 8 cut(s) 345, 797, 1380, 2497, 4000, 4537, 6008, 7278
VspI ATTAAT 2 cut(s) 2061, 7733
XagI CCTNNNNNAGG 2 cut(s) 3927, 4615
XbaI TCTAGA 2 cut(s) 4049, 5581
XcmI CCANNNNNNNNNTGG 3 cut(s) 5746, 6291, 6509
XmaJI CCTAGG 1 cut(s) 6011
XmiI GTMKAC 3 cut(s) 1199, 1861, 4549
XmnI GAANNNNTTC 6 cut(s) 1278, 1950, 2517, 6351, 6494, 7326
ZraI GACGTC 1 cut(s) 430
ZrmI AGTACT 4 cut(s) 1986, 3067, 6306, 7512
Zsp2I ATGCAT 6 cut(s) 1041, 1184, 1433, 3102, 6260, 7524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.