Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004527

Physical Location & Seq

Genomic Coordinates Forward (+)

26319170 .. 26320953

Gene Structure 1784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g35690.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGAGTTAGAATTCTACCGCAGGAATTCTCTGTTTCGCTTAATAGACACCGAAAGAAATATTGTACTAGAGTCGCCCAATGACCTCCATAACATCAACACCAATGACGAAGTGTATGATGATGAAGATCTCCAATTCAGCCCTTCAATGTCTTCAAGCCCCTACATAACTACAATGCTTACACTAATGCCACCCCCAAGCCCGGAGTCCCCATGGACCCTCTCCCCTCACCAAACCCCATCTCCTTCACTCCTTTACCAATGCATTGCATCCCTCTATCGCCCCGAAGGCTCCATCTCCTCCATTGCAGTCTCAAAGCAGCAAGGAGTGGTCTTTGCTGGCTCAGAGAGTACCCGAATCCATGTGTGGAGACAACCAGACCACAATGAATACGGATGTCTCAAGGCTACTTCAGGTGAGGTACGAGCCATTGCAGTTCATGGCAACATGCTCTTCACTGCACACAAAGACCACAAAATCCGAATGTGGAACTTCACGGTCACAGATCACATTTTCAAGTCCAAAAAGGTCTCTTCTTTCCCTAAAACAAGCTCGTTCACTTTATTTTCAAGATTTACAAACACCAAACAGCAACACAAAGAATGCATTTCCTGCTTGGCATATTATCAAACCGATGGTCTTGTCTATACTGGCTCCCATGACAGAACTGTCAAAGCGTGGCGACTTATAACCAATCACTGTGTGGACTCATTTGTGGCGCATGAAGACAATGTGAATGCAATAGTGGTGAACCAAGATGATGGGTGCGTTTTCACTTGCTCCTCAGATGGTTCTGTCAAAATCTGGAGAAGGGTATTCAGAGAAAACTCTCACACTCTCACCATGACTCTCAAATCCCAATTCCCAAATTCCCACCACCCTATAAACGCCATAGCCTTAAACGTATCATCCAACTCTTGCTTCCTCTACTCTGGTTCTTCAGATGGAACCATTAACTTTTGGGAGAAGGAGAAACTGACACACAGGTTTAACCATGGTGGGTTTTTACAAGGCCACCATTTTTCTGTTATGTGCTTAGTGGCCATTGAGAAGTTGATATTCAGTGGATCAGAGGATACTACAATTAGGGTTTGGAGGAGGGAAGAAGAGGGTTGTTTTCATGAGTGTTTGGCTGTGTTAGAGGGGCATAGAGGCCCTGTGAGGTGTTTGGCTGCTTGTTTAGAGATTGACACAGTTGTGAAAGGGTTTCTGGTTTATAGTGCGAGTTTGGATCAGACTTTTAAGGTGTGGAGGGTTAAGGTCTCGCCGGAAGAGGGAATGTGGTTGAACTCTCATATGGATCGGAGGAACAATGGGATCGAGGATGGAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0005875 GO:0005938 GO:0007154 GO:0007165 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0017016 GO:0017048 GO:0019899 GO:0019932 GO:0019933 GO:0019935 GO:0023052 GO:0031267 GO:0031592 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035556 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044380 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044877 GO:0045504 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051179 GO:0051640 GO:0051641 GO:0051642 GO:0051645 GO:0051716 GO:0061842 GO:0065007 GO:0070727 GO:0070840 GO:0071539 GO:0071944 GO:0072698 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1905508

KEGG Pathways

Metabolic & Signaling

ko04114 ko04120 ko04218 ko04310 ko04340 ko04341 ko04390 ko04624 ko04710 ko05131 map04114 map04120 map04218 map04310 map04340 map04341 map04390 map04624 map04710 map05131

Pfam Domains

Protein Families

WD40

Protein Analysis

445

Amino Acids

50.49

Weight (kDa)

6.42

Isoelectric Point (pI)

52.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	90 - 172	9.7e-06	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	92 - 190	2e-07	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	96 - 282	1.6e-21	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	108 - 271	9.5e-11	TEP-1 second beta-propeller
Beta-prop_ACSF4	PF13570	193 - 365	2.2e-06	Beta-alanine-activating enzyme, beta-propeller
WDR55	PF24796	193 - 420	5.3e-17	WDR55
WD40_CDC20-Fz	PF24807	196 - 330	3.5e-09	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	197 - 417	1.7e-07	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	197 - 283	6.6e-09	THOC3 beta-propeller domain
Beta-prop_EML	PF23409	197 - 391	9.8e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	197 - 270	2e-13	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	199 - 323	6.7e-14	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	199 - 324	8.9e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	200 - 286	6.7e-07	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	205 - 345	1.5e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	233 - 269	1e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	240 - 421	3.8e-12	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	282 - 420	3.3e-14	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	290 - 420	1.8e-13	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	294 - 391	8.4e-08	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	298 - 370	3.2e-06	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	299 - 374	6.6e-07	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	306 - 422	2.3e-10	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004527)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35690 FvH4_6g31961
malus_domestica	MD08G1115200.v1.1 MD09G1207500.v1.1 MD17G1188800.v1.1
prunus_persica	Prupe.1G449400_v2.0.a1 Prupe.3G063300_v2.0.a1
pyrus_communis	pycom09g12370
rosa_chinensis	RchiOBHm_Chr2g0139471 RchiOBHm_Chr6g0306111
rosa_laevigata	RLG00000010824 RLG00000019813
rosa_multiflora	Rmu_sc0000785.1_g000023 Rmu_sc0000903.1_g000022 Rmu_sc0001983.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105410 Rroxscaffold_7G00162050
rosa_rugosa	Rorug06G0282100 Rorug06G0346800
rosa_samantha	Rh2AG407500 Rh2BG418100 Rh2CG393500 Rh2DG426900 Rh6AG459200 Rh6BG441700 Rh6CG473700 Rh6DG459900
rosa_wichuraiana	Rw2G033010 Rw2G033270 Rw6G006540 Rw6G040030 Rw7G016590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	689
AasI	GACNNNNNNGTC	1 cut(s)	668
AciI	CCGC	1 cut(s)	19
AclWI	GGATC	4 cut(s)	1075, 1239, 1308, 1325
AcoI	YGGCCR	1 cut(s)	1041
AcsI	RAATTY	3 cut(s)	11, 25, 868
AcuI	CTGAAG	2 cut(s)	396, 924
AdeI	CACNNNGTG	1 cut(s)	703
AfaI	GTAC	3 cut(s)	66, 352, 423
AfiI	CCNNNNNNNGG	2 cut(s)	202, 1274
AgsI	TTSAA	5 cut(s)	147, 156, 517, 570, 1288
AjuI	GAANNNNNNNTTGG	2 cut(s)	905, 937
AleI	CACNNNNGTG	1 cut(s)	1196
AluBI	AGCT	1 cut(s)	552
AluI	AGCT	1 cut(s)	552
Alw26I	GTCTC	5 cut(s)	316, 364, 404, 535, 1267
AlwI	GGATC	4 cut(s)	1075, 1239, 1308, 1325
AoxI	GGCC	3 cut(s)	1012, 1041, 1153
ApeKI	GCWGC	2 cut(s)	319, 1172
ApoI	RAATTY	3 cut(s)	11, 25, 868
ArsI	GACNNNNNNTTYG	2 cut(s)	622, 654
AspLEI	GCGC	1 cut(s)	721
AspS9I	GGNCC	2 cut(s)	216, 1154
AsuC2I	CCSGG	1 cut(s)	203
AsuHPI	GGTGA	4 cut(s)	221, 428, 760, 832
AvaII	GGWCC	1 cut(s)	216
BalI	TGGCCA	1 cut(s)	1043
BbsI	GAAGAC	2 cut(s)	144, 732
BbvI	GCAGC	2 cut(s)	331, 1159
BccI	CCATC	7 cut(s)	247, 302, 629, 755, 782, 938, 1319
BciVI	GTATCC	1 cut(s)	1069
BcnI	CCSGG	1 cut(s)	203
BcoDI	GTCTC	5 cut(s)	316, 364, 404, 535, 1267
BfaI	CTAG	1 cut(s)	68
BfuI	GTATCC	1 cut(s)	1069
BglI	GCCNNNNNGGC	1 cut(s)	288
BglII	AGATCT	1 cut(s)	127
BisI	GCNGC	2 cut(s)	320, 1173
BlsI	GCNGC	2 cut(s)	321, 1174
Bme1390I	CCNGG	1 cut(s)	203
Bme18I	GGWCC	1 cut(s)	216
BmgT120I	GGNCC	2 cut(s)	216, 1154
BmiI	GGNNCC	4 cut(s)	218, 292, 655, 949
BmrFI	CCNGG	1 cut(s)	203
BmsI	GCATC	1 cut(s)	278
BpiI	GAAGAC	2 cut(s)	144, 732
BpmI	CTGGAG	1 cut(s)	826
BpuEI	CTTGAG	1 cut(s)	386
BpuMI	CCSGG	1 cut(s)	203
BsaBI	GATNNNNATC	1 cut(s)	126
BsaI	GGTCTC	2 cut(s)	535, 1267
BsaJI	CCNNGG	2 cut(s)	212, 994
BsaXI	ACNNNNNCTCC	2 cut(s)	226, 256
Bsc4I	CCNNNNNNNGG	2 cut(s)	202, 1274
Bse1I	ACTGG	1 cut(s)	655
Bse3DI	GCAATG	3 cut(s)	264, 303, 429
Bse8I	GATNNNNATC	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	212, 994
BseGI	GGATG	4 cut(s)	269, 401, 908, 1330
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	2 cut(s)	202, 1274
BseMI	GCAATG	3 cut(s)	264, 303, 429
BseMII	CTCAG	2 cut(s)	357, 798
BseNI	ACTGG	1 cut(s)	655
BseRI	GAGGAG	3 cut(s)	289, 772, 1111
BseXI	GCAGC	2 cut(s)	331, 1159
BsgI	GTGCAG	1 cut(s)	444
BshFI	GGCC	3 cut(s)	1014, 1043, 1155
BsiSI	CCGG	2 cut(s)	203, 1268
BslFI	GGGAC	1 cut(s)	193
BslI	CCNNNNNNNGG	2 cut(s)	202, 1274
BsmAI	GTCTC	5 cut(s)	316, 364, 404, 535, 1267
BsmFI	GGGAC	1 cut(s)	193
BsmI	GAATGC	2 cut(s)	608, 742
BsnI	GGCC	3 cut(s)	1014, 1043, 1155
Bso31I	GGTCTC	2 cut(s)	535, 1267
Bsp143I	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
Bsp19I	CCATGG	2 cut(s)	212, 994
BspACI	CCGC	1 cut(s)	19
BspANI	GGCC	3 cut(s)	1014, 1043, 1155
BspCNI	CTCAG	2 cut(s)	356, 797
BspHI	TCATGA	1 cut(s)	1120
BspLI	GGNNCC	4 cut(s)	218, 292, 655, 949
BspPI	GGATC	4 cut(s)	1075, 1239, 1308, 1325
BspQI	GCTCTTC	1 cut(s)	458
BspTNI	GGTCTC	2 cut(s)	535, 1267
BsrDI	GCAATG	3 cut(s)	264, 303, 429
BsrI	ACTGG	1 cut(s)	655
BssECI	CCNNGG	2 cut(s)	212, 994
BssMI	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
BssT1I	CCWWGG	2 cut(s)	212, 994
Bst4CI	ACNGT	4 cut(s)	499, 670, 701, 1195
Bst6I	CTCTTC	4 cut(s)	458, 538, 1101, 1266
BstAPI	GCANNNNNTGC	1 cut(s)	612
BstC8I	GCNNGC	1 cut(s)	340
BstDEI	CTNAG	3 cut(s)	343, 784, 1036
BstDSI	CCRYGG	2 cut(s)	212, 994
BstF5I	GGATG	4 cut(s)	269, 401, 908, 1330
BstHHI	GCGC	1 cut(s)	721
BstKTI	GATC	6 cut(s)	130, 508, 1070, 1234, 1303, 1320
BstMAI	GTCTC	5 cut(s)	316, 364, 404, 535, 1267
BstMBI	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
BstMWI	GCNNNNNNNGC	2 cut(s)	288, 612
BstNSI	RCATGY	1 cut(s)	451
BstSCI	CCNGG	1 cut(s)	201
BstV1I	GCAGC	2 cut(s)	331, 1159
BstV2I	GAAGAC	2 cut(s)	144, 732
BstX2I	RGATCY	1 cut(s)	127
BstXI	CCANNNNNNTGG	1 cut(s)	761
BstYI	RGATCY	1 cut(s)	127
BsuI	GTATCC	1 cut(s)	1069
BsuRI	GGCC	3 cut(s)	1014, 1043, 1155
BtgI	CCRYGG	2 cut(s)	212, 994
BtsCI	GGATG	4 cut(s)	269, 401, 908, 1330
BtsI	GCAGTG	1 cut(s)	456
BtsIMutI	CAGTG	3 cut(s)	456, 697, 1069
Cac8I	GCNNGC	1 cut(s)	340
CciI	TCATGA	1 cut(s)	1120
CfoI	GCGC	1 cut(s)	721
Cfr13I	GGNCC	2 cut(s)	216, 1154
Csp6I	GTAC	3 cut(s)	65, 351, 422
CviAII	CATG	9 cut(s)	213, 362, 440, 448, 659, 722, 844, 995, 1121
CviQI	GTAC	3 cut(s)	65, 351, 422
DdeI	CTNAG	3 cut(s)	343, 784, 1036
DpnI	GATC	6 cut(s)	129, 507, 1069, 1233, 1302, 1319
DpnII	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
DraIII	CACNNNGTG	1 cut(s)	703
DrdI	GACNNNNNNGTC	1 cut(s)	668
DseDI	GACNNNNNNGTC	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	1041
Eam1104I	CTCTTC	4 cut(s)	458, 538, 1101, 1266
EarI	CTCTTC	4 cut(s)	458, 538, 1101, 1266
Eco130I	CCWWGG	2 cut(s)	212, 994
Eco31I	GGTCTC	2 cut(s)	535, 1267
Eco47I	GGWCC	1 cut(s)	216
Eco57I	CTGAAG	2 cut(s)	396, 924
EcoO109I	RGGNCCY	1 cut(s)	1154
EcoRI	GAATTC	2 cut(s)	11, 25
EcoT14I	CCWWGG	2 cut(s)	212, 994
EcoT22I	ATGCAT	2 cut(s)	266, 608
ErhI	CCWWGG	2 cut(s)	212, 994
FaeI	CATG	9 cut(s)	216, 365, 443, 451, 662, 725, 847, 998, 1124
FaqI	GGGAC	1 cut(s)	193
FatI	CATG	9 cut(s)	212, 361, 439, 447, 658, 721, 843, 994, 1120
FauNDI	CATATG	1 cut(s)	1296
Fnu4HI	GCNGC	2 cut(s)	320, 1173
FokI	GGATG	3 cut(s)	256, 408, 895
Fsp4HI	GCNGC	2 cut(s)	320, 1173
FspBI	CTAG	1 cut(s)	68
GlaI	GCGC	1 cut(s)	720
GluI	GCNGC	2 cut(s)	320, 1173
GsuI	CTGGAG	1 cut(s)	826
HaeIII	GGCC	3 cut(s)	1014, 1043, 1155
HapII	CCGG	2 cut(s)	203, 1268
HhaI	GCGC	1 cut(s)	721
Hin1II	CATG	9 cut(s)	216, 365, 443, 451, 662, 725, 847, 998, 1124
Hin6I	GCGC	1 cut(s)	719
HinP1I	GCGC	1 cut(s)	719
HinfI	GANTC	5 cut(s)	71, 206, 357, 707, 847
HpaII	CCGG	2 cut(s)	203, 1268
HphI	GGTGA	4 cut(s)	221, 428, 760, 832
Hpy166II	GTNNAC	3 cut(s)	558, 706, 751
Hpy188I	TCNGA	8 cut(s)	346, 482, 787, 821, 943, 1072, 1236, 1305
Hpy188III	TCNNGA	3 cut(s)	570, 805, 1121
Hpy8I	GTNNAC	3 cut(s)	558, 706, 751
HpyAV	CCTTC	5 cut(s)	153, 255, 281, 804, 961
HpyCH4III	ACNGT	4 cut(s)	499, 670, 701, 1195
HpyCH4IV	ACGT	1 cut(s)	903
HpyCH4V	TGCA	7 cut(s)	264, 269, 308, 434, 461, 606, 740
HpyF10VI	GCNNNNNNNGC	2 cut(s)	288, 612
HpyF3I	CTNAG	3 cut(s)	343, 784, 1036
HpySE526I	ACGT	1 cut(s)	903
Hsp92II	CATG	9 cut(s)	216, 365, 443, 451, 662, 725, 847, 998, 1124
HspAI	GCGC	1 cut(s)	719
Kzo9I	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
LguI	GCTCTTC	1 cut(s)	458
LmnI	GCTCC	3 cut(s)	296, 659, 785
Lsp1109I	GCAGC	2 cut(s)	331, 1159
LweI	GCATC	1 cut(s)	278
MaeI	CTAG	1 cut(s)	68
MaeII	ACGT	1 cut(s)	903
MaeIII	GTNAC	1 cut(s)	499
MalI	GATC	6 cut(s)	129, 507, 1069, 1233, 1302, 1319
MboI	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
MboII	GAAGA	9 cut(s)	137, 144, 445, 525, 737, 930, 1115, 1118, 1283
MflI	RGATCY	1 cut(s)	127
MlsI	TGGCCA	1 cut(s)	1043
MluCI	AATT	6 cut(s)	11, 25, 134, 860, 868, 1083
MluNI	TGGCCA	1 cut(s)	1043
MlyI	GAGTC	4 cut(s)	80, 215, 701, 841
MmeI	TCCRAC	1 cut(s)	936
Mox20I	TGGCCA	1 cut(s)	1043
Mph1103I	ATGCAT	2 cut(s)	266, 608
MscI	TGGCCA	1 cut(s)	1043
MseI	TTAA	6 cut(s)	41, 899, 954, 990, 1242, 1257
MslI	CAYNNNNRTG	1 cut(s)	1196
Msp20I	TGGCCA	1 cut(s)	1043
MspI	CCGG	2 cut(s)	203, 1268
MspR9I	CCNGG	1 cut(s)	203
Mva1269I	GAATGC	2 cut(s)	608, 742
MwoI	GCNNNNNNNGC	2 cut(s)	288, 612
NciI	CCSGG	1 cut(s)	203
NcoI	CCATGG	2 cut(s)	212, 994
NdeI	CATATG	1 cut(s)	1296
NdeII	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
NlaIII	CATG	9 cut(s)	216, 365, 443, 451, 662, 725, 847, 998, 1124
NlaIV	GGNNCC	4 cut(s)	218, 292, 655, 949
NmuCI	GTSAC	1 cut(s)	499
NsiI	ATGCAT	2 cut(s)	266, 608
NspI	RCATGY	1 cut(s)	451
OliI	CACNNNNGTG	1 cut(s)	1196
PagI	TCATGA	1 cut(s)	1120
PciSI	GCTCTTC	1 cut(s)	458
PctI	GAATGC	2 cut(s)	608, 742
PfeI	GAWTC	1 cut(s)	357
PkrI	GCNGC	2 cut(s)	321, 1174
PleI	GAGTC	4 cut(s)	79, 214, 701, 841
PpsI	GAGTC	4 cut(s)	79, 214, 701, 841
PsiI	TTATAA	1 cut(s)	689
PspN4I	GGNNCC	4 cut(s)	218, 292, 655, 949
PspPI	GGNCC	2 cut(s)	216, 1154
PsuI	RGATCY	1 cut(s)	127
RsaI	GTAC	3 cut(s)	66, 352, 423
RsaNI	GTAC	3 cut(s)	65, 351, 422
RseI	CAYNNNNRTG	1 cut(s)	1196
SapI	GCTCTTC	1 cut(s)	458
SaqAI	TTAA	6 cut(s)	41, 899, 954, 990, 1242, 1257
SatI	GCNGC	2 cut(s)	320, 1173
Sau3AI	GATC	6 cut(s)	127, 505, 1067, 1231, 1300, 1317
Sau96I	GGNCC	2 cut(s)	216, 1154
SchI	GAGTC	4 cut(s)	80, 215, 701, 841
ScrFI	CCNGG	1 cut(s)	203
SfaNI	GCATC	1 cut(s)	278
SinI	GGWCC	1 cut(s)	216
SmiMI	CAYNNNNRTG	1 cut(s)	1196
SmlI	CTYRAG	1 cut(s)	401
SmoI	CTYRAG	1 cut(s)	401
Sse9I	AATT	6 cut(s)	11, 25, 134, 860, 868, 1083
SsiI	CCGC	1 cut(s)	19
SspI	AATATT	1 cut(s)	61
SspMI	CTAG	1 cut(s)	68
StyD4I	CCNGG	1 cut(s)	201
StyI	CCWWGG	2 cut(s)	212, 994
TaaI	ACNGT	4 cut(s)	499, 670, 701, 1195
TaiI	ACGT	1 cut(s)	906
TaqI	TCGA	1 cut(s)	1320
TasI	AATT	6 cut(s)	11, 25, 134, 860, 868, 1083
TatI	WGTACW	1 cut(s)	64
TfiI	GAWTC	1 cut(s)	357
Tru1I	TTAA	6 cut(s)	41, 899, 954, 990, 1242, 1257
Tru9I	TTAA	6 cut(s)	41, 899, 954, 990, 1242, 1257
TscAI	CASTG	3 cut(s)	463, 704, 1069
TseFI	GTSAC	1 cut(s)	499
TseI	GCWGC	2 cut(s)	319, 1172
Tsp45I	GTSAC	1 cut(s)	499
TspDTI	ATGAA	5 cut(s)	138, 402, 428, 738, 1109
TspGWI	ACGGA	1 cut(s)	408
TspRI	CASTG	3 cut(s)	463, 704, 1069
VpaK11BI	GGWCC	1 cut(s)	216
XapI	RAATTY	3 cut(s)	11, 25, 868
XceI	RCATGY	1 cut(s)	451
XcmI	CCANNNNNNNNNTGG	1 cut(s)	700
XspI	CTAG	1 cut(s)	68
Zsp2I	ATGCAT	2 cut(s)	266, 608

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.