Rroxscaffold_2G00105410

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004527
Physical Location & Seq
Forward (+)
28827492 .. 28828880
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105410.1

Sequence Viewer

Length: 1389 bp
ATGGAGTTTCATGGAGATCAAAGAAACCTCTGGGGCTTCATGGATGAAGAAAGAAAGAGCATCAAGTTACCACGGAGACTTTCCTTTGGTAGTAACCAACAACAAAGTTCATCATCCGAAGTACCTAACATTCACTTAGCTTCTGCAGCAAGACCATCAACTGCTGCTTCAGAACCATCAATTGATGCAATTCCTCCCAGACCTATGAGCCCCGGCACGCCATGGACACTCTCTCCGGTGCGCACTTCTCCTTCCCAACCTCTTCTTTATCATTGCATAGCCTCCCTCCACCGCCATGAAGGAAACATTTTCTCCATCACACTCTCAAAAGATTTCATATTCACTGGCTCAGAAAGTAGCCGGATTCATGCTTGGAAGCAACCAGATTGCACCGAGATAGGTTACATAAAGGCTACTTCGGGTCAGGTTCGAGCCATATTGGCCTATGGGAAAATGCTGTTCACCACACATGGTGACTACAAAATTCGGGTATGGGATGCATCATCCACAGAGAATTTTAGACCTAAGAAGATCACAAGCTTACCACAGAGAAATCGATTCTCTAATTTGTTTTCGAAAAAGAGCAGCCAGCAACACAATGATCACATCTCTTGCCTCGCTTACAACAAGGAAGACAAGCTGTTGTACACAGGTTCGTGGGACAAATCAGTTAAAGCTTGGAAGATCAACGAAAAGCATTGTGTCGACTCGTTCATTGCTCATGAAGGTCATGTGAATGGGATTGTGATTAACCAGGAAGACGGGTGTGTTTTCACTTGCTCATCAGATGGCTCAGTCAAAATTTGGAGAAGGGTATTTGGTGAAAGTTCGCACATACTCACCATGACCCTAAAGTTTCAACTTTCACCAGTAAATGCCTTGGCATTGAGCTTGTCAAATAGCACCACCCTCCTCTACTCTGGCTCATCGGATGGACTCATAAACTTCTGGGAAAAAGAGAAGATGTCTGGAAGATACAACCATGGTGGGTTTCTGCAAGGTCATCATTTCGCCGTTCTTTGCTTGGTAGCCTTGGGAGAACTAATCTTCAGTGGCTCTGAGGATGCAACCATTAGGGTTTGGAGGAGAGAGGAAGGAAACTGCATCCATTCTTGTCTTTCTGTCATTGAGGGACATCACGGGCCAGTTAGATGCTTAGCAGTGTCTTTGGAAACAGAGAATGTGGTAAGGGTTAAGGGTTTGTTGGTTTATAGCGCAAGCTTAGACCAAACTTTTAAGGTGTGGAGGGTTAAGGTTTTTCCAACGGAGAAACTAAACTTGGATGAGTCTGCAAAAGACCCACAAAGAGAGATTTTGGAGTGCGAGACAAGTCCAGTGCTGTCACCTTCATGGGTGGAGAAAAAGCTTCAGGGAAACTTTTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

462

Amino Acids

51.9

Weight (kDa)

8.54

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 150 - 270 1.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 150 - 282 7.2e-12 THOC3 beta-propeller domain
WDR55 PF24796 192 - 420 1.9e-14 WDR55
WD40_CDC20-Fz PF24807 195 - 320 2.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 196 - 281 6e-08 TEP-1 second beta-propeller
Beta-prop_ACSF4 PF13570 196 - 372 4.8e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR5 PF25175 197 - 282 2.2e-16 WDR5 beta-propeller domain
WD40 PF00400 197 - 227 6.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 198 - 270 9.2e-13 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 198 - 432 3.6e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 198 - 361 8.5e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 199 - 283 5.1e-07 EIF3I
WD40_Prp19 PF24814 200 - 320 3.3e-14 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 201 - 281 1.4e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 203 - 320 8e-12 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 205 - 341 1.9e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 283 - 418 1e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 294 - 389 2.2e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 295 - 369 7.7e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 296 - 418 7.7e-13 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 302 - 416 1.4e-10 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 346 - 418 1.3e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 242
AccI GTMKAC 1 cut(s) 705
AciI CCGC 1 cut(s) 292
AcsI RAATTY 3 cut(s) 483, 514, 801
AcuI CTGAAG 3 cut(s) 153, 1033, 1352
AdeI CACNNNGTG 1 cut(s) 473
AfaI GTAC 2 cut(s) 123, 647
AgsI TTSAA 1 cut(s) 860
AjnI CCWGG 1 cut(s) 753
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
AluBI AGCT 7 cut(s) 140, 540, 640, 677, 891, 1221, 1366
AluI AGCT 7 cut(s) 140, 540, 640, 677, 891, 1221, 1366
Alw26I GTCTC 2 cut(s) 70, 1319
AoxI GGCC 2 cut(s) 441, 1142
ApeKI GCWGC 3 cut(s) 146, 164, 585
ApoI RAATTY 3 cut(s) 483, 514, 801
AspLEI GCGC 2 cut(s) 243, 1217
AspS9I GGNCC 1 cut(s) 1142
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 6 cut(s) 454, 485, 832, 833, 858, 1335
AsuII TTCGAA 1 cut(s) 575
BanII GRGCYC 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 639, 765
BbvI GCAGC 3 cut(s) 151, 158, 597
BccI CCATC 5 cut(s) 163, 184, 323, 782, 926
BceAI ACGGC 1 cut(s) 998
BciT130I CCWGG 1 cut(s) 755
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 70, 1319
BfmI CTRYAG 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 147, 165, 586
BlpI GCTNAGC 1 cut(s) 1156
BlsI GCNGC 3 cut(s) 148, 166, 587
Bme1390I CCNGG 2 cut(s) 213, 755
BmgT120I GGNCC 1 cut(s) 1142
BmrFI CCNGG 2 cut(s) 213, 755
BmsI GCATC 7 cut(s) 69, 175, 487, 509, 1054, 1113, 1142
BpiI GAAGAC 2 cut(s) 639, 765
Bpu1102I GCTNAGC 1 cut(s) 1156
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 556
BsaJI CCNNGG 6 cut(s) 71, 211, 221, 879, 982, 1032
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 296, 326
Bse1I ACTGG 5 cut(s) 349, 869, 1145, 1334, 1384
Bse3DI GCAATG 2 cut(s) 271, 714
BseBI CCWGG 1 cut(s) 755
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 6 cut(s) 71, 211, 221, 879, 982, 1032
BseGI GGATG 8 cut(s) 49, 113, 502, 503, 937, 1069, 1104, 1288
BseMI GCAATG 2 cut(s) 271, 714
BseMII CTCAG 3 cut(s) 363, 807, 1050
BseNI ACTGG 5 cut(s) 349, 869, 1145, 1334, 1384
BseRI GAGGAG 2 cut(s) 902, 1099
BseXI GCAGC 3 cut(s) 151, 158, 597
BshFI GGCC 2 cut(s) 443, 1144
BshVI ATCGAT 1 cut(s) 556
BsiSI CCGG 3 cut(s) 213, 236, 361
BslFI GGGAC 2 cut(s) 674, 1146
BsmAI GTCTC 2 cut(s) 70, 1319
BsmFI GGGAC 2 cut(s) 674, 1146
BsnI GGCC 2 cut(s) 443, 1144
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 4 cut(s) 16, 531, 601, 684
Bsp1720I GCTNAGC 1 cut(s) 1156
Bsp19I CCATGG 2 cut(s) 221, 982
BspACI CCGC 1 cut(s) 292
BspANI GGCC 2 cut(s) 443, 1144
BspCNI CTCAG 3 cut(s) 362, 806, 1051
BspDI ATCGAT 1 cut(s) 556
BspHI TCATGA 1 cut(s) 721
BspMAI CTGCAG 1 cut(s) 148
BspT104I TTCGAA 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 271, 714
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 5 cut(s) 349, 869, 1145, 1334, 1384
BssECI CCNNGG 6 cut(s) 71, 211, 221, 879, 982, 1032
BssMI GATC 4 cut(s) 16, 531, 601, 684
BssT1I CCWWGG 4 cut(s) 221, 879, 982, 1032
Bst2UI CCWGG 1 cut(s) 755
Bst6I CTCTTC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 218, 590, 1219
BstDEI CTNAG 7 cut(s) 136, 349, 525, 793, 1059, 1156, 1222
BstDSI CCRYGG 3 cut(s) 71, 221, 982
BstF5I GGATG 8 cut(s) 49, 113, 502, 503, 937, 1069, 1104, 1288
BstHHI GCGC 2 cut(s) 243, 1217
BstKTI GATC 4 cut(s) 19, 534, 604, 687
BstMAI GTCTC 2 cut(s) 70, 1319
BstMBI GATC 4 cut(s) 16, 531, 601, 684
BstMWI GCNNNNNNNGC 2 cut(s) 146, 440
BstNI CCWGG 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 211, 753
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 151, 158, 597
BstV2I GAAGAC 2 cut(s) 639, 765
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 2 cut(s) 443, 1144
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 3 cut(s) 71, 221, 982
BtsCI GGATG 8 cut(s) 49, 113, 502, 503, 937, 1069, 1104, 1288
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 4 cut(s) 342, 1057, 1167, 1341
Cac8I GCNNGC 3 cut(s) 218, 590, 1219
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 243, 1217
Cfr13I GGNCC 1 cut(s) 1142
ClaI ATCGAT 1 cut(s) 556
Csp6I GTAC 2 cut(s) 122, 646
CspCI CAANNNNNGTGG 2 cut(s) 967, 1002
CviQI GTAC 2 cut(s) 122, 646
DdeI CTNAG 7 cut(s) 136, 349, 525, 793, 1059, 1156, 1222
DpnI GATC 4 cut(s) 18, 533, 603, 686
DpnII GATC 4 cut(s) 16, 531, 601, 684
DraIII CACNNNGTG 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco130I CCWWGG 4 cut(s) 221, 879, 982, 1032
Eco24I GRGCYC 1 cut(s) 212
Eco57I CTGAAG 3 cut(s) 153, 1033, 1352
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 4 cut(s) 221, 879, 982, 1032
EcoT22I ATGCAT 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 4 cut(s) 221, 879, 982, 1032
FaqI GGGAC 2 cut(s) 674, 1146
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 3 cut(s) 147, 165, 586
FokI GGATG 8 cut(s) 56, 100, 490, 509, 944, 1076, 1091, 1295
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 3 cut(s) 147, 165, 586
FspAI RTGCGCAY 1 cut(s) 242
FspI TGCGCA 1 cut(s) 242
GlaI GCGC 2 cut(s) 242, 1216
GluI GCNGC 3 cut(s) 147, 165, 586
HaeIII GGCC 2 cut(s) 443, 1144
HapII CCGG 3 cut(s) 213, 236, 361
HhaI GCGC 2 cut(s) 243, 1217
Hin6I GCGC 2 cut(s) 241, 1215
HinP1I GCGC 2 cut(s) 241, 1215
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 4 cut(s) 538, 675, 1219, 1364
HinfI GANTC 5 cut(s) 364, 558, 707, 936, 1286
HpaII CCGG 3 cut(s) 213, 236, 361
HphI GGTGA 6 cut(s) 454, 485, 832, 833, 858, 1335
Hpy166II GTNNAC 3 cut(s) 462, 648, 706
Hpy188I TCNGA 6 cut(s) 118, 172, 352, 787, 931, 1060
Hpy188III TCNNGA 2 cut(s) 722, 969
Hpy8I GTNNAC 3 cut(s) 462, 648, 706
HpyAV CCTTC 6 cut(s) 261, 293, 719, 804, 1088, 1356
HpyCH4V TGCA 9 cut(s) 146, 188, 276, 390, 500, 997, 1067, 1104, 1292
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 440
HpyF3I CTNAG 7 cut(s) 136, 349, 525, 793, 1059, 1156, 1222
HspAI GCGC 2 cut(s) 241, 1215
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 4 cut(s) 16, 531, 601, 684
Lsp1109I GCAGC 3 cut(s) 151, 158, 597
LweI GCATC 7 cut(s) 69, 175, 487, 509, 1054, 1113, 1142
MaeIII GTNAC 5 cut(s) 66, 92, 401, 473, 1341
MalI GATC 4 cut(s) 18, 533, 603, 686
MboI GATC 4 cut(s) 16, 531, 601, 684
MboII GAAGA 9 cut(s) 59, 254, 541, 644, 694, 770, 973, 984, 1039
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 6 cut(s) 180, 189, 483, 514, 565, 801
MlyI GAGTC 3 cut(s) 701, 930, 1295
MmeI TCCRAC 1 cut(s) 1286
Mph1103I ATGCAT 1 cut(s) 502
MseI TTAA 5 cut(s) 672, 750, 1194, 1236, 1251
MslI CAYNNNNRTG 3 cut(s) 294, 735, 1348
MspI CCGG 3 cut(s) 213, 236, 361
MspR9I CCNGG 2 cut(s) 213, 755
MunI CAATTG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 755
MwoI GCNNNNNNNGC 2 cut(s) 146, 440
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 2 cut(s) 221, 982
NdeII GATC 4 cut(s) 16, 531, 601, 684
NmuCI GTSAC 2 cut(s) 473, 1341
NsbI TGCGCA 1 cut(s) 242
NsiI ATGCAT 1 cut(s) 502
NspV TTCGAA 1 cut(s) 575
PagI TCATGA 1 cut(s) 721
PfeI GAWTC 2 cut(s) 364, 558
PkrI GCNGC 3 cut(s) 148, 166, 587
PleI GAGTC 3 cut(s) 701, 930, 1294
PpsI GAGTC 3 cut(s) 701, 930, 1294
Psp6I CCWGG 1 cut(s) 753
PspGI CCWGG 1 cut(s) 753
PspPI GGNCC 1 cut(s) 1142
PstI CTGCAG 1 cut(s) 148
RsaI GTAC 2 cut(s) 123, 647
RsaNI GTAC 2 cut(s) 122, 646
RseI CAYNNNNRTG 3 cut(s) 294, 735, 1348
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 5 cut(s) 672, 750, 1194, 1236, 1251
SatI GCNGC 3 cut(s) 147, 165, 586
Sau3AI GATC 4 cut(s) 16, 531, 601, 684
Sau96I GGNCC 1 cut(s) 1142
SchI GAGTC 3 cut(s) 701, 930, 1295
ScrFI CCNGG 2 cut(s) 213, 755
SduI GDGCHC 1 cut(s) 212
SfaNI GCATC 7 cut(s) 69, 175, 487, 509, 1054, 1113, 1142
SfcI CTRYAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 575
SmiMI CAYNNNNRTG 3 cut(s) 294, 735, 1348
Sse9I AATT 6 cut(s) 180, 189, 483, 514, 565, 801
SsiI CCGC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 211, 753
StyI CCWWGG 4 cut(s) 221, 879, 982, 1032
TaqI TCGA 4 cut(s) 430, 556, 575, 705
TasI AATT 6 cut(s) 180, 189, 483, 514, 565, 801
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 364, 558
Tru1I TTAA 5 cut(s) 672, 750, 1194, 1236, 1251
Tru9I TTAA 5 cut(s) 672, 750, 1194, 1236, 1251
TscAI CASTG 4 cut(s) 349, 1057, 1167, 1341
TseFI GTSAC 2 cut(s) 473, 1341
TseI GCWGC 3 cut(s) 146, 164, 585
Tsp45I GTSAC 2 cut(s) 473, 1341
TspDTI ATGAA 9 cut(s) 28, 60, 99, 312, 325, 356, 703, 738, 1338
TspGWI ACGGA 2 cut(s) 88, 1280
TspRI CASTG 4 cut(s) 349, 1057, 1167, 1341
XapI RAATTY 3 cut(s) 483, 514, 801
XmiI GTMKAC 1 cut(s) 705
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.