FvH4_6g31961

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004527
Physical Location & Seq
Reverse (-)
25067683 .. 25069029
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31961.t1

Sequence Viewer

Length: 1347 bp
ATGGATGAGGAAAGAAAGAGCATCAAGTTACCTCGGAGACTTTCTTTCGGTAGTAACCAACAAAGTTCATCATCCGAAGCACCTAGCATTCACTTTGCTTCTGCAGCAAGACCATCTACTGCTGTTTCTGAACCATCAATTGATGCAATTCCTCCACGACCTATGAGCCCCGGCACGCCATGGACACTCTCCCCAGTGCGTACTTCTTCTTCTCAACCTCTTCTTTATCATTGCATAGCCTCCCTCCACCGCCATGAAGGTAACATTTTCTCCATCACACTCTCAAAAGATTTTATATTCACTGGCTCAGAAAGTAGCCGGATTCATGCTTGGAAGCAACCAGATTGTACCGAGATAGGTTACATAAAGGCTACTTCGGGTCAGGTACGAGCTATCTTGGCCTATGGGAGAATGTTGTTCACCACACATGGTGACTACAAAATCCGGGTATGGGATGCATCATCCGCAGAGAATTTTAGACCTAAGAAGATCACAACCTTACCACAGAGAAACAGATTCTCTAATTTGTTTTCGAAAAAGAGCAGCCAGCAACACACTGATCACATCTCTTGCCTCGCTTACAACAAGGAAGACAAGCTGTTGTACACAGGCTCATGGGACAAATCAGTTAAAGCTTGGAAGATCAACGAAAAGCGTTGTGTCGACTCGTTCATTGCTCATGAAGGTCATGTGAATGGAATTGTCATCAACCAGGAAGATGGGTGTGTTTTCACTTGCTCATCAGATGGCTCAGTCAAAATTTGGAGAAGGGTGTTTGGTGAAAGCTCACATATACTCACCATGACACTGAAGTTTCAACTTTCACCAGTGAATGCCTTGGCATTGAGCTTGTCAAATAGCACCACCCTCCTCTACTCTGGCTCATCAGATGGGCTCATAAACTTCTGGGAGAAAGAGAAGATGTCTGGAAGATATAACCATGGTGGGTTTCTGCAAGGTCACCATTTCGCCGTTCTTTGCTTGGTTGCCTTGGGAGAACTAATCTTCAGTGGCTCTGAGGATGCAACCATTAGGGTTTGGAGGAGAGAGGAAGGAAATTGCTTCCATTCTTGTCTTTCTGTCATTGAGGGGCATCACGGGCCGGTTAGATGCTTAGCAGTGTCTTTGGAAACAGAGAATGTGGTCAGGGTTAAGGGTTTGTTGGTTTATAGTGCAAGCTTAGATCAAACTTTTAAGGTGTGGAGGGTTAAGGTTTTTCCAACTGAGAAACTAAACTTGGATGAGTCTACAAAAGACCCACATAGAGAGATTTTGGAGTGTGAGACAAGTCCTGTGCTGTCACCTTCATGGGTGGAGAAAAAGCTTCAGGGCAACTTTTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

449

Amino Acids

50.24

Weight (kDa)

8.77

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 94 - 257 1.9e-10 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 136 - 256 1.3e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 136 - 268 3.8e-12 THOC3 beta-propeller domain
WDR55 PF24796 178 - 406 2.3e-14 WDR55
Beta-prop_IP5PC_F PF23754 181 - 257 8e-06 IP5P C-F beta-propeller
WD40_CDC20-Fz PF24807 181 - 306 2.3e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 183 - 268 4.9e-17 WDR5 beta-propeller domain
WD40_Gbeta PF25391 183 - 347 3.1e-12 G protein beta WD-40 repeat protein
WD40 PF00400 183 - 213 3.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 184 - 256 4.2e-12 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 185 - 267 5.6e-09 WDHD1 first WD40 domain
EIF3I PF24805 185 - 268 6.7e-07 EIF3I
WD40_Prp19 PF24814 186 - 306 1.7e-14 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 189 - 306 1.1e-11 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 191 - 327 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 219 - 255 9.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 268 - 404 1.2e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 282 - 404 9.1e-13 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 288 - 404 1.2e-10 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 332 - 404 1.1e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 663, 1247
AciI CCGC 2 cut(s) 250, 465
AcsI RAATTY 2 cut(s) 472, 759
AcuI CTGAAG 3 cut(s) 830, 991, 1310
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 4 cut(s) 202, 349, 387, 605
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 1 cut(s) 818
AjnI CCWGG 1 cut(s) 711
AjuI GAANNNNNNNTTGG 2 cut(s) 1220, 1252
AluBI AGCT 7 cut(s) 392, 598, 635, 786, 849, 1179, 1324
AluI AGCT 7 cut(s) 392, 598, 635, 786, 849, 1179, 1324
Alw26I GTCTC 2 cut(s) 31, 1277
AoxI GGCC 2 cut(s) 399, 1100
ApeKI GCWGC 2 cut(s) 104, 543
ApoI RAATTY 2 cut(s) 472, 759
AspS9I GGNCC 1 cut(s) 1100
AsuC2I CCSGG 2 cut(s) 171, 446
AsuHPI GGTGA 7 cut(s) 412, 443, 790, 791, 816, 953, 1293
AsuII TTCGAA 1 cut(s) 533
BanII GRGCYC 2 cut(s) 170, 897
BarI GAAGNNNNNNTAC 2 cut(s) 193, 225
BbsI GAAGAC 1 cut(s) 597
BbvI GCAGC 2 cut(s) 116, 555
BccI CCATC 6 cut(s) 121, 142, 281, 713, 740, 884
BceAI ACGGC 1 cut(s) 956
BciT130I CCWGG 1 cut(s) 713
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 171, 446
BcoDI GTCTC 2 cut(s) 31, 1277
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 102
BisI GCNGC 2 cut(s) 105, 544
BlpI GCTNAGC 1 cut(s) 1114
BlsI GCNGC 2 cut(s) 106, 545
Bme1390I CCNGG 3 cut(s) 171, 446, 713
BmgT120I GGNCC 1 cut(s) 1100
BmrFI CCNGG 3 cut(s) 171, 446, 713
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 7 cut(s) 30, 133, 445, 467, 1012, 1100, 1102
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 1114
Bpu14I TTCGAA 1 cut(s) 533
BpuMI CCSGG 2 cut(s) 171, 446
BsaJI CCNNGG 6 cut(s) 32, 169, 179, 837, 940, 990
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 1102
Bse1I ACTGG 4 cut(s) 194, 307, 827, 1342
Bse3DI GCAATG 2 cut(s) 229, 672
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 6 cut(s) 32, 169, 179, 837, 940, 990
BseGI GGATG 6 cut(s) 10, 71, 460, 461, 1027, 1246
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 2 cut(s) 229, 672
BseMII CTCAG 4 cut(s) 321, 765, 1008, 1215
BseNI ACTGG 4 cut(s) 194, 307, 827, 1342
BseRI GAGGAG 2 cut(s) 860, 1057
BseXI GCAGC 2 cut(s) 116, 555
BshFI GGCC 2 cut(s) 401, 1102
BsiSI CCGG 4 cut(s) 171, 319, 445, 1103
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 2 cut(s) 31, 1277
BsmFI GGGAC 1 cut(s) 632
BsmI GAATGC 2 cut(s) 87, 838
BsnI GGCC 2 cut(s) 401, 1102
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 170, 897
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 4 cut(s) 489, 559, 642, 1183
Bsp1720I GCTNAGC 1 cut(s) 1114
Bsp19I CCATGG 2 cut(s) 179, 940
BspACI CCGC 2 cut(s) 250, 465
BspANI GGCC 2 cut(s) 401, 1102
BspCNI CTCAG 4 cut(s) 320, 764, 1009, 1216
BspHI TCATGA 1 cut(s) 679
BspMAI CTGCAG 1 cut(s) 106
BspT104I TTCGAA 1 cut(s) 533
BsrDI GCAATG 2 cut(s) 229, 672
BsrFI RCCGGY 1 cut(s) 1102
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 4 cut(s) 194, 307, 827, 1342
BssAI RCCGGY 1 cut(s) 1102
BssECI CCNNGG 6 cut(s) 32, 169, 179, 837, 940, 990
BssMI GATC 4 cut(s) 489, 559, 642, 1183
BssT1I CCWWGG 4 cut(s) 179, 837, 940, 990
Bst2UI CCWGG 1 cut(s) 713
Bst6I CTCTTC 1 cut(s) 225
BstAUI TGTACA 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 176, 548, 1177
BstDEI CTNAG 7 cut(s) 307, 483, 751, 1017, 1114, 1180, 1224
BstDSI CCRYGG 2 cut(s) 179, 940
BstEII GGTNACC 1 cut(s) 959
BstF5I GGATG 6 cut(s) 10, 71, 460, 461, 1027, 1246
BstKTI GATC 4 cut(s) 492, 562, 645, 1186
BstMAI GTCTC 2 cut(s) 31, 1277
BstMBI GATC 4 cut(s) 489, 559, 642, 1183
BstMWI GCNNNNNNNGC 4 cut(s) 104, 398, 464, 1099
BstNI CCWGG 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 959
BstSCI CCNGG 3 cut(s) 169, 444, 711
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 116, 555
BstV2I GAAGAC 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 719
BsuRI GGCC 2 cut(s) 401, 1102
BtgI CCRYGG 2 cut(s) 179, 940
BtsCI GGATG 6 cut(s) 10, 71, 460, 461, 1027, 1246
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 7 cut(s) 201, 300, 555, 806, 834, 1015, 1125
Cac8I GCNNGC 3 cut(s) 176, 548, 1177
CciI TCATGA 1 cut(s) 679
Cfr10I RCCGGY 1 cut(s) 1102
Cfr13I GGNCC 1 cut(s) 1100
Csp6I GTAC 4 cut(s) 201, 348, 386, 604
CviQI GTAC 4 cut(s) 201, 348, 386, 604
DdeI CTNAG 7 cut(s) 307, 483, 751, 1017, 1114, 1180, 1224
DpnI GATC 4 cut(s) 491, 561, 644, 1185
DpnII GATC 4 cut(s) 489, 559, 642, 1183
DraIII CACNNNGTG 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 4 cut(s) 179, 837, 940, 990
Eco24I GRGCYC 2 cut(s) 170, 897
Eco57I CTGAAG 3 cut(s) 830, 991, 1310
Eco91I GGTNACC 1 cut(s) 959
EcoO65I GGTNACC 1 cut(s) 959
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 4 cut(s) 179, 837, 940, 990
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 2 cut(s) 170, 897
ErhI CCWWGG 4 cut(s) 179, 837, 940, 990
FaqI GGGAC 1 cut(s) 632
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 2 cut(s) 663, 1247
Fnu4HI GCNGC 2 cut(s) 105, 544
FokI GGATG 6 cut(s) 17, 58, 448, 467, 1034, 1253
FriOI GRGCYC 2 cut(s) 170, 897
Fsp4HI GCNGC 2 cut(s) 105, 544
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 105, 544
HaeIII GGCC 2 cut(s) 401, 1102
HapII CCGG 4 cut(s) 171, 319, 445, 1103
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 3 cut(s) 633, 1177, 1322
HinfI GANTC 4 cut(s) 322, 516, 665, 1244
HpaII CCGG 4 cut(s) 171, 319, 445, 1103
HphI GGTGA 7 cut(s) 412, 443, 790, 791, 816, 953, 1293
Hpy166II GTNNAC 4 cut(s) 420, 606, 664, 1248
Hpy188I TCNGA 7 cut(s) 36, 76, 130, 310, 745, 889, 1018
Hpy188III TCNNGA 2 cut(s) 680, 927
Hpy8I GTNNAC 4 cut(s) 420, 606, 664, 1248
HpyAV CCTTC 5 cut(s) 251, 677, 762, 1046, 1314
HpyCH4V TGCA 7 cut(s) 104, 146, 234, 458, 955, 1025, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 398, 464, 1099
HpyF3I CTNAG 7 cut(s) 307, 483, 751, 1017, 1114, 1180, 1224
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 4 cut(s) 489, 559, 642, 1183
Lsp1109I GCAGC 2 cut(s) 116, 555
LweI GCATC 7 cut(s) 30, 133, 445, 467, 1012, 1100, 1102
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 7 cut(s) 27, 53, 260, 359, 431, 959, 1299
MalI GATC 4 cut(s) 491, 561, 644, 1185
MboI GATC 4 cut(s) 489, 559, 642, 1183
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 170, 897
MluCI AATT 7 cut(s) 138, 147, 472, 523, 699, 759, 1057
MlyI GAGTC 2 cut(s) 659, 1253
MmeI TCCRAC 1 cut(s) 1244
Mph1103I ATGCAT 1 cut(s) 460
MseI TTAA 4 cut(s) 630, 1152, 1194, 1209
MslI CAYNNNNRTG 3 cut(s) 252, 693, 1306
MspI CCGG 4 cut(s) 171, 319, 445, 1103
MspR9I CCNGG 3 cut(s) 171, 446, 713
MunI CAATTG 1 cut(s) 138
Mva1269I GAATGC 2 cut(s) 87, 838
MvaI CCWGG 1 cut(s) 713
MwoI GCNNNNNNNGC 4 cut(s) 104, 398, 464, 1099
NciI CCSGG 2 cut(s) 171, 446
NcoI CCATGG 2 cut(s) 179, 940
NdeII GATC 4 cut(s) 489, 559, 642, 1183
NmuCI GTSAC 3 cut(s) 431, 959, 1299
NsiI ATGCAT 1 cut(s) 460
NspV TTCGAA 1 cut(s) 533
PagI TCATGA 1 cut(s) 679
PctI GAATGC 2 cut(s) 87, 838
PfeI GAWTC 2 cut(s) 322, 516
PkrI GCNGC 2 cut(s) 106, 545
PleI GAGTC 2 cut(s) 659, 1252
PpsI GAGTC 2 cut(s) 659, 1252
Psp6I CCWGG 1 cut(s) 711
PspEI GGTNACC 1 cut(s) 959
PspGI CCWGG 1 cut(s) 711
PspPI GGNCC 1 cut(s) 1100
PstI CTGCAG 1 cut(s) 106
RsaI GTAC 4 cut(s) 202, 349, 387, 605
RsaNI GTAC 4 cut(s) 201, 348, 386, 604
RseI CAYNNNNRTG 3 cut(s) 252, 693, 1306
SalI GTCGAC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 630, 1152, 1194, 1209
SatI GCNGC 2 cut(s) 105, 544
Sau3AI GATC 4 cut(s) 489, 559, 642, 1183
Sau96I GGNCC 1 cut(s) 1100
SchI GAGTC 2 cut(s) 659, 1253
ScrFI CCNGG 3 cut(s) 171, 446, 713
SduI GDGCHC 2 cut(s) 170, 897
SfaNI GCATC 7 cut(s) 30, 133, 445, 467, 1012, 1100, 1102
SfcI CTRYAG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 533
SmiMI CAYNNNNRTG 3 cut(s) 252, 693, 1306
Sse9I AATT 7 cut(s) 138, 147, 472, 523, 699, 759, 1057
SsiI CCGC 2 cut(s) 250, 465
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 169, 444, 711
StyI CCWWGG 4 cut(s) 179, 837, 940, 990
TaqI TCGA 2 cut(s) 533, 663
TasI AATT 7 cut(s) 138, 147, 472, 523, 699, 759, 1057
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 2 cut(s) 322, 516
Tru1I TTAA 4 cut(s) 630, 1152, 1194, 1209
Tru9I TTAA 4 cut(s) 630, 1152, 1194, 1209
TscAI CASTG 7 cut(s) 201, 307, 562, 813, 834, 1015, 1125
TseFI GTSAC 3 cut(s) 431, 959, 1299
TseI GCWGC 2 cut(s) 104, 543
Tsp45I GTSAC 3 cut(s) 431, 959, 1299
TspDTI ATGAA 6 cut(s) 57, 270, 314, 661, 696, 1296
TspRI CASTG 7 cut(s) 201, 307, 562, 813, 834, 1015, 1125
XapI RAATTY 2 cut(s) 472, 759
XmiI GTMKAC 2 cut(s) 663, 1247
XspI CTAG 1 cut(s) 84
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.