Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0004527

Physical Location & Seq

Genomic Coordinates Reverse (-)

64228100 .. 64229422

Gene Structure 1323 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG459200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGAATTGGAATTCTATCGCAGGAATTCTCTGTTTCACTTCATAGATATCGACAGAAATAATTTAATAGAATCACCCACTGACTCGCCCAATATCAACACGGATCACGACGTGTGTGATGATGAAGATCTCCAATTCGGTCCTTCATTGTCTTCAAGCCCCCACACAACCACAATGCTTACACTAATGCCACCCCCAAGCCCAGAGTCCCCATGGACCCTCTCCCCTCACCAAACCCCATCCCCTTCGCTCCTTTACCACTGCATTGCCTCCCTCTATCGCCGCGAAGGCTCCATCTCCTCCATTGCAGTCTCAAAGCAGCAAGGAGTAGTCTTCACTGGCTCAGAGAGTACCCGAGTTCGTGTGTGGAGACAACCAGACCACATTGAATACGGCTGTCTCAATGCGACTTCAGGTGAAGTACGAGCTATTGCAACTCATGGTAATATGCTTTTCACCTCACACAAAGACCACAAAATCCGAATGTGGAACTTCACGGTCACAGATCACAATTTCAAGTCCAAAAAGGTTTCTTCTCTCCCCAAAACAAGCTCTTTACCTTTACTTTCAAGATTTATAAACACCAAACAGCAACACAAAGAATGCATTTCCTGCTTGGCATATTATAAAACCGATGGTCTGTTGTATACTGGCTCCCATGACAGAACAGTCAAAGCATGGCGGCTTATAACCAATCACTGTGTGGACTCATTTGTGGCACATGAAGACAATGTGAATGCAATGGTGGTGAACCAAGATGATGGGTGCGTTTTCACTTGCTCCTCAGATGGTTCTGTCAAACTTTGGAGAAGGGTTTTCAGAGAAAACTCTCACACTCTCACCATGACTCTCAAATGCCAATTCCCAAATTCCAACTACCCTGTAAACGCCATAGCCTTAAACACATCATCCAACTCTTGCTTCCTCTACTCTGGTTCTGCAGACGGAACCATTAGTTTCTGGGAGAAGGAGAAACTGACCCACAGGTTTAACCATGGTGGGTTTTTACAGGGCCACCATTTTTCAGTTCTGTGTTTAGTGGCCATCGAGAAGTTGATTTTCAGTGGATCAGAGGATACTACAATTAGGGTTTGGAGGAGGGAAGAAGGAGGTTGTTTTCATGAATGTTTGGCTGTGTTGGAGGGGCATAGAGGCCCTGTGAGGTGTTTGGCTGCTTGTTTGGAGATTGAGAAAGTTGTGAAGGGTTTTTTGGTTTATAGTGCCAGTTTGGATCAAACTTTTAAGGTGTGGAGTGTTAAGGTCTCGCCGGAAGAGGGAATGGGGTTGAACTGTTATATGGATCAACACTGGGATGCAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0005875 GO:0005938 GO:0007154 GO:0007165 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0017016 GO:0017048 GO:0019899 GO:0019932 GO:0019933 GO:0019935 GO:0023052 GO:0031267 GO:0031592 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035556 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044380 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044877 GO:0045504 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051179 GO:0051640 GO:0051641 GO:0051642 GO:0051645 GO:0051716 GO:0061842 GO:0065007 GO:0070727 GO:0070840 GO:0071539 GO:0071944 GO:0072698 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1905508

KEGG Pathways

Metabolic & Signaling

ko04114 ko04120 ko04218 ko04310 ko04340 ko04341 ko04390 ko04624 ko04710 ko05131 map04114 map04120 map04218 map04310 map04340 map04341 map04390 map04624 map04710 map05131

Pfam Domains

Protein Families

WD40

Protein Analysis

440

Amino Acids

49.73

Weight (kDa)

6.51

Isoelectric Point (pI)

53.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	95 - 282	6.5e-21	WDR5 beta-propeller domain
EIF3I	PF24805	95 - 270	6.3e-08	EIF3I
Beta-prop_TEP1_2nd	PF25047	108 - 271	1.1e-10	TEP-1 second beta-propeller
Beta-prop_ACSF4	PF13570	193 - 365	1.6e-07	Beta-alanine-activating enzyme, beta-propeller
WDR55	PF24796	195 - 420	6.1e-16	WDR55
Beta-prop_WDR3_1st	PF25173	197 - 270	4.6e-14	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	197 - 324	2.5e-09	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	198 - 417	4e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	199 - 323	9.1e-11	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	199 - 282	6.2e-09	THOC3 beta-propeller domain
WD40_Prp19	PF24814	199 - 324	1.1e-12	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	201 - 286	4.4e-06	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	205 - 366	1.8e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	233 - 269	1.5e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	240 - 420	1.7e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	291 - 420	1.1e-12	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	295 - 418	7.4e-08	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	295 - 420	1.7e-13	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	307 - 421	7.8e-10	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	352 - 421	7.7e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004527)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35690 FvH4_6g31961
malus_domestica	MD08G1115200.v1.1 MD09G1207500.v1.1 MD17G1188800.v1.1
prunus_persica	Prupe.1G449400_v2.0.a1 Prupe.3G063300_v2.0.a1
pyrus_communis	pycom09g12370
rosa_chinensis	RchiOBHm_Chr2g0139471 RchiOBHm_Chr6g0306111
rosa_laevigata	RLG00000010824 RLG00000019813
rosa_multiflora	Rmu_sc0000785.1_g000023 Rmu_sc0000903.1_g000022 Rmu_sc0001983.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105410 Rroxscaffold_7G00162050
rosa_rugosa	Rorug06G0282100 Rorug06G0346800
rosa_samantha	Rh2AG407500 Rh2BG418100 Rh2CG393500 Rh2DG426900 Rh6AG459200 Rh6BG441700 Rh6CG473700 Rh6DG459900
rosa_wichuraiana	Rw2G033010 Rw2G033270 Rw6G006540 Rw6G040030 Rw7G016590

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	578, 627, 689
AasI	GACNNNNNNGTC	1 cut(s)	668
AccI	GTMKAC	1 cut(s)	647
AccII	CGCG	1 cut(s)	285
AciI	CCGC	2 cut(s)	283, 682
AclWI	GGATC	4 cut(s)	111, 1075, 1239, 1308
AcoI	YGGCCR	1 cut(s)	1041
AcsI	RAATTY	3 cut(s)	11, 25, 868
AcuI	CTGAAG	1 cut(s)	396
AdeI	CACNNNGTG	2 cut(s)	112, 703
AfaI	GTAC	2 cut(s)	352, 423
AfiI	CCNNNNNNNGG	1 cut(s)	1274
AflIII	ACRYGT	1 cut(s)	111
AgsI	TTSAA	5 cut(s)	156, 389, 517, 570, 1288
AjiI	CACGTC	1 cut(s)	112
AjuI	GAANNNNNNNTTGG	4 cut(s)	905, 937, 1193, 1225
AluBI	AGCT	2 cut(s)	428, 552
AluI	AGCT	2 cut(s)	428, 552
Alw26I	GTCTC	4 cut(s)	316, 364, 404, 1267
AlwI	GGATC	4 cut(s)	111, 1075, 1239, 1308
Ama87I	CYCGRG	1 cut(s)	354
AoxI	GGCC	3 cut(s)	1012, 1041, 1153
ApeKI	GCWGC	2 cut(s)	319, 1172
ApoI	RAATTY	3 cut(s)	11, 25, 868
AspS9I	GGNCC	4 cut(s)	140, 216, 1012, 1154
AsuHPI	GGTGA	6 cut(s)	66, 221, 428, 448, 760, 832
AvaI	CYCGRG	1 cut(s)	354
AvaII	GGWCC	2 cut(s)	140, 216
BalI	TGGCCA	1 cut(s)	1043
BbsI	GAAGAC	3 cut(s)	144, 325, 732
BbvI	GCAGC	2 cut(s)	331, 1159
BccI	CCATC	6 cut(s)	247, 302, 629, 755, 782, 1052
BceAI	ACGGC	1 cut(s)	409
BciVI	GTATCC	1 cut(s)	1069
BcoDI	GTCTC	4 cut(s)	316, 364, 404, 1267
BfmI	CTRYAG	1 cut(s)	939
BfuI	GTATCC	1 cut(s)	1069
BglI	GCCNNNNNGGC	1 cut(s)	288
BglII	AGATCT	1 cut(s)	127
BisI	GCNGC	4 cut(s)	283, 320, 683, 1173
BlsI	GCNGC	4 cut(s)	284, 321, 684, 1174
Bme18I	GGWCC	2 cut(s)	140, 216
BmeT110I	CYCGRG	1 cut(s)	354
BmgBI	CACGTC	1 cut(s)	112
BmgT120I	GGNCC	4 cut(s)	140, 216, 1012, 1154
BmiI	GGNNCC	4 cut(s)	218, 292, 655, 949
BmrI	ACTGGG	1 cut(s)	1318
BmsI	GCATC	1 cut(s)	1303
BmuI	ACTGGG	1 cut(s)	1318
BpiI	GAAGAC	3 cut(s)	144, 325, 732
BsaBI	GATNNNNATC	1 cut(s)	126
BsaI	GGTCTC	1 cut(s)	1267
BsaJI	CCNNGG	2 cut(s)	212, 994
Bsc4I	CCNNNNNNNGG	1 cut(s)	1274
Bse1I	ACTGG	4 cut(s)	343, 655, 1224, 1313
Bse3DI	GCAATG	4 cut(s)	264, 303, 747, 1323
Bse8I	GATNNNNATC	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	212, 994
BseGI	GGATG	3 cut(s)	239, 908, 1318
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	1 cut(s)	1274
BseMI	GCAATG	4 cut(s)	264, 303, 747, 1323
BseMII	CTCAG	2 cut(s)	357, 798
BseNI	ACTGG	4 cut(s)	343, 655, 1224, 1313
BseRI	GAGGAG	3 cut(s)	289, 772, 1111
BseXI	GCAGC	2 cut(s)	331, 1159
Bsh1236I	CGCG	1 cut(s)	285
BshFI	GGCC	3 cut(s)	1014, 1043, 1155
BsiHKCI	CYCGRG	1 cut(s)	354
BsiSI	CCGG	1 cut(s)	1268
BslFI	GGGAC	1 cut(s)	193
BslI	CCNNNNNNNGG	1 cut(s)	1274
BsmAI	GTCTC	4 cut(s)	316, 364, 404, 1267
BsmFI	GGGAC	1 cut(s)	193
BsmI	GAATGC	2 cut(s)	608, 742
BsnI	GGCC	3 cut(s)	1014, 1043, 1155
Bso31I	GGTCTC	1 cut(s)	1267
BsoBI	CYCGRG	1 cut(s)	354
Bsp143I	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
Bsp19I	CCATGG	2 cut(s)	212, 994
BspACI	CCGC	2 cut(s)	283, 682
BspANI	GGCC	3 cut(s)	1014, 1043, 1155
BspCNI	CTCAG	2 cut(s)	356, 797
BspFNI	CGCG	1 cut(s)	285
BspHI	TCATGA	1 cut(s)	1120
BspLI	GGNNCC	4 cut(s)	218, 292, 655, 949
BspMAI	CTGCAG	1 cut(s)	943
BspPI	GGATC	4 cut(s)	111, 1075, 1239, 1308
BspTNI	GGTCTC	1 cut(s)	1267
BsrDI	GCAATG	4 cut(s)	264, 303, 747, 1323
BsrI	ACTGG	4 cut(s)	343, 655, 1224, 1313
BssECI	CCNNGG	2 cut(s)	212, 994
BssMI	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
BssNAI	GTATAC	1 cut(s)	648
BssT1I	CCWWGG	2 cut(s)	212, 994
Bst1107I	GTATAC	1 cut(s)	648
Bst4CI	ACNGT	4 cut(s)	499, 670, 701, 1292
Bst6I	CTCTTC	1 cut(s)	1266
BstAPI	GCANNNNNTGC	1 cut(s)	612
BstDEI	CTNAG	2 cut(s)	343, 784
BstDSI	CCRYGG	2 cut(s)	212, 994
BstF5I	GGATG	3 cut(s)	239, 908, 1318
BstFNI	CGCG	1 cut(s)	285
BstKTI	GATC	6 cut(s)	106, 130, 508, 1070, 1234, 1303
BstMAI	GTCTC	4 cut(s)	316, 364, 404, 1267
BstMBI	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
BstMWI	GCNNNNNNNGC	2 cut(s)	288, 612
BstSFI	CTRYAG	1 cut(s)	939
BstUI	CGCG	1 cut(s)	285
BstV1I	GCAGC	2 cut(s)	331, 1159
BstV2I	GAAGAC	3 cut(s)	144, 325, 732
BstX2I	RGATCY	1 cut(s)	127
BstXI	CCANNNNNNTGG	1 cut(s)	761
BstYI	RGATCY	1 cut(s)	127
BstZ17I	GTATAC	1 cut(s)	648
BsuI	GTATCC	1 cut(s)	1069
BsuRI	GGCC	3 cut(s)	1014, 1043, 1155
BtgI	CCRYGG	2 cut(s)	212, 994
BtrI	CACGTC	1 cut(s)	112
BtsCI	GGATG	3 cut(s)	239, 908, 1318
BtsI	GCAGTG	1 cut(s)	259
BtsIMutI	CAGTG	6 cut(s)	78, 259, 336, 697, 1069, 1306
CciI	TCATGA	1 cut(s)	1120
Cfr13I	GGNCC	4 cut(s)	140, 216, 1012, 1154
Csp6I	GTAC	2 cut(s)	351, 422
CviAII	CATG	8 cut(s)	213, 440, 659, 678, 722, 844, 995, 1121
CviQI	GTAC	2 cut(s)	351, 422
DdeI	CTNAG	2 cut(s)	343, 784
DpnI	GATC	6 cut(s)	105, 129, 507, 1069, 1233, 1302
DpnII	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
DraIII	CACNNNGTG	2 cut(s)	112, 703
DrdI	GACNNNNNNGTC	1 cut(s)	668
DseDI	GACNNNNNNGTC	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	1041
Eam1104I	CTCTTC	1 cut(s)	1266
EarI	CTCTTC	1 cut(s)	1266
Eco130I	CCWWGG	2 cut(s)	212, 994
Eco31I	GGTCTC	1 cut(s)	1267
Eco32I	GATATC	1 cut(s)	49
Eco47I	GGWCC	2 cut(s)	140, 216
Eco57I	CTGAAG	1 cut(s)	396
Eco88I	CYCGRG	1 cut(s)	354
EcoO109I	RGGNCCY	1 cut(s)	1154
EcoRI	GAATTC	2 cut(s)	11, 25
EcoRV	GATATC	1 cut(s)	49
EcoT14I	CCWWGG	2 cut(s)	212, 994
EcoT22I	ATGCAT	1 cut(s)	608
ErhI	CCWWGG	2 cut(s)	212, 994
FaeI	CATG	8 cut(s)	216, 443, 662, 681, 725, 847, 998, 1124
FaqI	GGGAC	1 cut(s)	193
FatI	CATG	8 cut(s)	212, 439, 658, 677, 721, 843, 994, 1120
FblI	GTMKAC	1 cut(s)	647
Fnu4HI	GCNGC	4 cut(s)	283, 320, 683, 1173
FokI	GGATG	2 cut(s)	226, 895
Fsp4HI	GCNGC	4 cut(s)	283, 320, 683, 1173
GluI	GCNGC	4 cut(s)	283, 320, 683, 1173
HaeIII	GGCC	3 cut(s)	1014, 1043, 1155
HapII	CCGG	1 cut(s)	1268
Hin1II	CATG	8 cut(s)	216, 443, 662, 681, 725, 847, 998, 1124
HinfI	GANTC	5 cut(s)	71, 83, 206, 707, 847
HpaII	CCGG	1 cut(s)	1268
HphI	GGTGA	6 cut(s)	66, 221, 428, 448, 760, 832
Hpy166II	GTNNAC	4 cut(s)	648, 706, 751, 886
Hpy188I	TCNGA	5 cut(s)	346, 482, 787, 821, 1072
Hpy188III	TCNNGA	4 cut(s)	107, 570, 1048, 1121
Hpy8I	GTNNAC	4 cut(s)	648, 706, 751, 886
Hpy99I	CGWCG	1 cut(s)	113
HpyAV	CCTTC	7 cut(s)	153, 255, 281, 804, 961, 1100, 1195
HpyCH4III	ACNGT	4 cut(s)	499, 670, 701, 1292
HpyCH4IV	ACGT	1 cut(s)	111
HpyCH4V	TGCA	7 cut(s)	264, 308, 434, 606, 740, 941, 1316
HpyF10VI	GCNNNNNNNGC	2 cut(s)	288, 612
HpyF3I	CTNAG	2 cut(s)	343, 784
HpySE526I	ACGT	1 cut(s)	111
Hsp92II	CATG	8 cut(s)	216, 443, 662, 681, 725, 847, 998, 1124
Kzo9I	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
LmnI	GCTCC	4 cut(s)	255, 296, 659, 785
Lsp1109I	GCAGC	2 cut(s)	331, 1159
LweI	GCATC	1 cut(s)	1303
MaeII	ACGT	1 cut(s)	111
MaeIII	GTNAC	1 cut(s)	499
MalI	GATC	6 cut(s)	105, 129, 507, 1069, 1233, 1302
MboI	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
MboII	GAAGA	7 cut(s)	137, 144, 325, 525, 737, 1115, 1283
MflI	RGATCY	1 cut(s)	127
MlsI	TGGCCA	1 cut(s)	1043
MluCI	AATT	9 cut(s)	5, 11, 25, 61, 134, 511, 860, 868, 1083
MluNI	TGGCCA	1 cut(s)	1043
MlyI	GAGTC	4 cut(s)	77, 215, 701, 841
MmeI	TCCRAC	3 cut(s)	897, 936, 1119
Mox20I	TGGCCA	1 cut(s)	1043
Mph1103I	ATGCAT	1 cut(s)	608
MscI	TGGCCA	1 cut(s)	1043
MseI	TTAA	5 cut(s)	65, 899, 990, 1242, 1257
MslI	CAYNNNNRTG	1 cut(s)	1311
Msp20I	TGGCCA	1 cut(s)	1043
MspI	CCGG	1 cut(s)	1268
Mva1269I	GAATGC	2 cut(s)	608, 742
MvnI	CGCG	1 cut(s)	285
MwoI	GCNNNNNNNGC	2 cut(s)	288, 612
NcoI	CCATGG	2 cut(s)	212, 994
NdeII	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
NlaIII	CATG	8 cut(s)	216, 443, 662, 681, 725, 847, 998, 1124
NlaIV	GGNNCC	4 cut(s)	218, 292, 655, 949
NmuCI	GTSAC	1 cut(s)	499
NsiI	ATGCAT	1 cut(s)	608
PagI	TCATGA	1 cut(s)	1120
PctI	GAATGC	2 cut(s)	608, 742
PfeI	GAWTC	1 cut(s)	71
PkrI	GCNGC	4 cut(s)	284, 321, 684, 1174
PleI	GAGTC	4 cut(s)	77, 214, 701, 841
PpsI	GAGTC	4 cut(s)	77, 214, 701, 841
PsiI	TTATAA	3 cut(s)	578, 627, 689
PspN4I	GGNNCC	4 cut(s)	218, 292, 655, 949
PspPI	GGNCC	4 cut(s)	140, 216, 1012, 1154
PstI	CTGCAG	1 cut(s)	943
PsuI	RGATCY	1 cut(s)	127
RsaI	GTAC	2 cut(s)	352, 423
RsaNI	GTAC	2 cut(s)	351, 422
RseI	CAYNNNNRTG	1 cut(s)	1311
SaqAI	TTAA	5 cut(s)	65, 899, 990, 1242, 1257
SatI	GCNGC	4 cut(s)	283, 320, 683, 1173
Sau3AI	GATC	6 cut(s)	103, 127, 505, 1067, 1231, 1300
Sau96I	GGNCC	4 cut(s)	140, 216, 1012, 1154
SchI	GAGTC	4 cut(s)	77, 215, 701, 841
SfaNI	GCATC	1 cut(s)	1303
SfcI	CTRYAG	1 cut(s)	939
SinI	GGWCC	2 cut(s)	140, 216
SmiMI	CAYNNNNRTG	1 cut(s)	1311
Sse9I	AATT	9 cut(s)	5, 11, 25, 61, 134, 511, 860, 868, 1083
SsiI	CCGC	2 cut(s)	283, 682
StyI	CCWWGG	2 cut(s)	212, 994
TaaI	ACNGT	4 cut(s)	499, 670, 701, 1292
TaiI	ACGT	1 cut(s)	114
TaqI	TCGA	2 cut(s)	51, 1047
TaqII	GACCGA	1 cut(s)	128
TasI	AATT	9 cut(s)	5, 11, 25, 61, 134, 511, 860, 868, 1083
TauI	GCSGC	2 cut(s)	285, 685
TfiI	GAWTC	1 cut(s)	71
Tru1I	TTAA	5 cut(s)	65, 899, 990, 1242, 1257
Tru9I	TTAA	5 cut(s)	65, 899, 990, 1242, 1257
TscAI	CASTG	6 cut(s)	85, 266, 343, 704, 1069, 1313
TseFI	GTSAC	1 cut(s)	499
TseI	GCWGC	2 cut(s)	319, 1172
Tsp45I	GTSAC	1 cut(s)	499
TspDTI	ATGAA	6 cut(s)	31, 135, 138, 738, 1109, 1137
TspGWI	ACGGA	2 cut(s)	116, 960
TspRI	CASTG	6 cut(s)	85, 266, 343, 704, 1069, 1313
VpaK11BI	GGWCC	2 cut(s)	140, 216
XapI	RAATTY	3 cut(s)	11, 25, 868
XcmI	CCANNNNNNNNNTGG	2 cut(s)	210, 700
XmiI	GTMKAC	1 cut(s)	647
Zsp2I	ATGCAT	1 cut(s)	608

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.