Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004527

Physical Location & Seq

Genomic Coordinates Forward (+)

10436131 .. 10437813

Gene Structure 1683 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1115200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGATGATAAATGATGATCTTGATTTGGTTCCAAAAAGTTTTGGCACAACACTCTACAACTGCATTGCCTCCCTCCACCTCCGCGAAGGCACCATATACTCCATTGCAATTTCAAGGCAACATGGAGTAGTATTTACTGGCTCCAAGAGTACCAGAATCCGTGCATGGAGGCAACCGGACTACATGGAATTTGGATGCCTCCAGACAGCTTCAGAAGGTTTGTTGTACACTGGCTCATATGACACAACAGTTAAAGCCTGGAGAGTTTTAACCAAAAACTCTGTCGATTCGTTTGTGGCCCATGAACACGATGTCAATGCAATTTTGGTGAACCAAGAGGATGGGTGTGTCTTTACTTGCTCCTCTGATGTGTCTGTAAAAATTTGGAGAAGGGTTTTGGGAGAAAACTCTCACACTCTCACCATGACACTGAAATTCCAAAACTACCCCGTAAATGCCATAGCCCTAAGTACTTCATCACTTAACTCTAGTTGCTTCCTGTACTCTGGGTCTTCAGATGGAACCATTAATTTTTGGGAGAAGGAGAAGTTGACCTACAGATTTAACCATTGTGGGTTTTTACAAGGTCACAACTTTGCAGTCCTATGCCTAGTGGCTGTCGAGAAGTTGGTATTTAGTGGGTCAGAGGATACAACAATTAGGGTTTGGAGGAGAGGCGAGGGGAGTTGCTTCCATGAGTGTTTGGCTGTATTGGATGGGCATAGAGGACCTGTCGGGTGTTTGGCTGCTTGTTTAGAGACGGACAGAATTGTAGTGGGGTTTTTGGTTTATAGTGCAAGTTTGGACCAGAGTTTTAAGGTGTGGAGGGTAAAGGGGTCACCTGAAGAGAAAATGGGATTGAACTATAGAGACAGGAGTTTTTCTAACCCTGTGTTGTCCCCAATTCATGGGTTGAGAGAAAGCTGCTTCAAAGGTAGACATTTTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0005875 GO:0005938 GO:0007154 GO:0007165 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0017016 GO:0017048 GO:0019899 GO:0019932 GO:0019933 GO:0019935 GO:0023052 GO:0031267 GO:0031592 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035556 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044380 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044877 GO:0045504 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051179 GO:0051640 GO:0051641 GO:0051642 GO:0051645 GO:0051716 GO:0061842 GO:0065007 GO:0070727 GO:0070840 GO:0071539 GO:0071944 GO:0072698 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1905508

KEGG Pathways

Metabolic & Signaling

ko04114 ko04120 ko04218 ko04310 ko04340 ko04341 ko04390 ko04624 ko04710 ko05131 map04114 map04120 map04218 map04310 map04340 map04341 map04390 map04624 map04710 map05131

Pfam Domains

Protein Families

WD40

Protein Analysis

317

Amino Acids

35.42

Weight (kDa)

7.56

Isoelectric Point (pI)

36.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	30 - 181	1.2e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	53 - 157	1.1e-11	WDR5 beta-propeller domain
WDR55	PF24796	54 - 278	4.2e-13	WDR55
WD40_Prp19	PF24814	69 - 182	1.5e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	70 - 182	3.7e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	71 - 130	7.2e-08	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	73 - 182	7.8e-08	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	73 - 223	1.8e-08	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	74 - 143	1.1e-06	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	140 - 278	2e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	142 - 278	7.2e-13	WDR5 beta-propeller domain
WD40_Prp19	PF24814	162 - 250	8e-07	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	163 - 279	1e-10	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	166 - 233	8.5e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004527)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35690 FvH4_6g31961
malus_domestica	MD08G1115200.v1.1 MD09G1207500.v1.1 MD17G1188800.v1.1
prunus_persica	Prupe.1G449400_v2.0.a1 Prupe.3G063300_v2.0.a1
pyrus_communis	pycom09g12370
rosa_chinensis	RchiOBHm_Chr2g0139471 RchiOBHm_Chr6g0306111
rosa_laevigata	RLG00000010824 RLG00000019813
rosa_multiflora	Rmu_sc0000785.1_g000023 Rmu_sc0000903.1_g000022 Rmu_sc0001983.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105410 Rroxscaffold_7G00162050
rosa_rugosa	Rorug06G0282100 Rorug06G0346800
rosa_samantha	Rh2AG407500 Rh2BG418100 Rh2CG393500 Rh2DG426900 Rh6AG459200 Rh6BG441700 Rh6CG473700 Rh6DG459900
rosa_wichuraiana	Rw2G033010 Rw2G033270 Rw6G006540 Rw6G040030 Rw7G016590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	89
AccB7I	CCANNNNNTGG	1 cut(s)	908
AccI	GTMKAC	1 cut(s)	937
AccII	CGCG	1 cut(s)	84
AciI	CCGC	1 cut(s)	82
AcsI	RAATTY	3 cut(s)	188, 381, 434
AcuI	CTGAAG	3 cut(s)	195, 498, 864
AfaI	GTAC	4 cut(s)	151, 227, 472, 503
AfiI	CCNNNNNNNGG	1 cut(s)	908
AgsI	TTSAA	3 cut(s)	114, 862, 931
AjnI	CCWGG	1 cut(s)	257
AluBI	AGCT	2 cut(s)	209, 924
AluI	AGCT	2 cut(s)	209, 924
Alw26I	GTCTC	2 cut(s)	752, 864
AoxI	GGCC	1 cut(s)	297
ApeKI	GCWGC	2 cut(s)	746, 924
ApoI	RAATTY	3 cut(s)	188, 381, 434
ArsI	GACNNNNNNTTYG	2 cut(s)	267, 299
AseI	ATTAAT	1 cut(s)	528
AspS9I	GGNCC	3 cut(s)	298, 728, 805
AsuHPI	GGTGA	3 cut(s)	340, 412, 831
AvaII	GGWCC	2 cut(s)	728, 805
BanI	GGYRCC	1 cut(s)	89
BarI	GAAGNNNNNNTAC	2 cut(s)	539, 571
BbsI	GAAGAC	1 cut(s)	504
BbvI	GCAGC	2 cut(s)	733, 911
BccI	CCATC	3 cut(s)	335, 512, 710
BcgI	CGANNNNNNTGC	2 cut(s)	299, 333
BciT130I	CCWGG	1 cut(s)	259
BciVI	GTATCC	1 cut(s)	643
BcoDI	GTCTC	2 cut(s)	752, 864
BfaI	CTAG	2 cut(s)	489, 611
BfmI	CTRYAG	2 cut(s)	556, 865
BfuI	GTATCC	1 cut(s)	643
BisI	GCNGC	2 cut(s)	747, 925
BlsI	GCNGC	2 cut(s)	748, 926
BmcAI	AGTACT	1 cut(s)	472
Bme1390I	CCNGG	1 cut(s)	259
Bme18I	GGWCC	2 cut(s)	728, 805
BmgT120I	GGNCC	3 cut(s)	298, 728, 805
BmiI	GGNNCC	4 cut(s)	30, 91, 142, 523
BmrFI	CCNGG	1 cut(s)	259
BmsI	GCATC	1 cut(s)	185
BpiI	GAAGAC	1 cut(s)	504
BpmI	CTGGAG	2 cut(s)	185, 280
BsaWI	WCCGGW	1 cut(s)	175
Bsc4I	CCNNNNNNNGG	1 cut(s)	908
Bse1I	ACTGG	2 cut(s)	142, 235
Bse3DI	GCAATG	2 cut(s)	63, 102
BseBI	CCWGG	1 cut(s)	259
BseGI	GGATG	3 cut(s)	200, 346, 721
BseLI	CCNNNNNNNGG	1 cut(s)	908
BseMI	GCAATG	2 cut(s)	63, 102
BseNI	ACTGG	2 cut(s)	142, 235
BseRI	GAGGAG	2 cut(s)	352, 685
BseXI	GCAGC	2 cut(s)	733, 911
Bsh1236I	CGCG	1 cut(s)	84
BshFI	GGCC	1 cut(s)	299
BshNI	GGYRCC	1 cut(s)	89
BsiSI	CCGG	1 cut(s)	176
BslFI	GGGAC	1 cut(s)	883
BslI	CCNNNNNNNGG	1 cut(s)	908
BsmAI	GTCTC	2 cut(s)	752, 864
BsmBI	CGTCTC	1 cut(s)	752
BsmFI	GGGAC	1 cut(s)	883
BsnI	GGCC	1 cut(s)	299
Bsp1407I	TGTACA	1 cut(s)	225
Bsp143I	GATC	1 cut(s)	16
BspACI	CCGC	1 cut(s)	82
BspANI	GGCC	1 cut(s)	299
BspFNI	CGCG	1 cut(s)	84
BspLI	GGNNCC	4 cut(s)	30, 91, 142, 523
BspT107I	GGYRCC	1 cut(s)	89
BsrDI	GCAATG	2 cut(s)	63, 102
BsrGI	TGTACA	1 cut(s)	225
BsrI	ACTGG	2 cut(s)	142, 235
BssMI	GATC	1 cut(s)	16
Bst2UI	CCWGG	1 cut(s)	259
Bst4CI	ACNGT	1 cut(s)	250
Bst6I	CTCTTC	1 cut(s)	840
BstAUI	TGTACA	1 cut(s)	225
BstDEI	CTNAG	1 cut(s)	467
BstEII	GGTNACC	1 cut(s)	837
BstF5I	GGATG	3 cut(s)	200, 346, 721
BstFNI	CGCG	1 cut(s)	84
BstKTI	GATC	1 cut(s)	19
BstMAI	GTCTC	2 cut(s)	752, 864
BstMBI	GATC	1 cut(s)	16
BstNI	CCWGG	1 cut(s)	259
BstPI	GGTNACC	1 cut(s)	837
BstSCI	CCNGG	1 cut(s)	257
BstSFI	CTRYAG	2 cut(s)	556, 865
BstUI	CGCG	1 cut(s)	84
BstV1I	GCAGC	2 cut(s)	733, 911
BstV2I	GAAGAC	1 cut(s)	504
BstXI	CCANNNNNNTGG	1 cut(s)	341
BsuI	GTATCC	1 cut(s)	643
BsuRI	GGCC	1 cut(s)	299
BtsCI	GGATG	3 cut(s)	200, 346, 721
BtsIMutI	CAGTG	2 cut(s)	228, 428
Cfr13I	GGNCC	3 cut(s)	298, 728, 805
Csp6I	GTAC	4 cut(s)	150, 226, 471, 502
CviAII	CATG	7 cut(s)	122, 165, 184, 302, 424, 695, 908
CviQI	GTAC	4 cut(s)	150, 226, 471, 502
DdeI	CTNAG	1 cut(s)	467
DpnI	GATC	1 cut(s)	18
DpnII	GATC	1 cut(s)	16
Eam1104I	CTCTTC	1 cut(s)	840
EarI	CTCTTC	1 cut(s)	840
Eco47I	GGWCC	2 cut(s)	728, 805
Eco57I	CTGAAG	3 cut(s)	195, 498, 864
Eco91I	GGTNACC	1 cut(s)	837
EcoO109I	RGGNCCY	1 cut(s)	728
EcoO65I	GGTNACC	1 cut(s)	837
EcoRII	CCWGG	1 cut(s)	257
Esp3I	CGTCTC	1 cut(s)	752
FaeI	CATG	7 cut(s)	125, 168, 187, 305, 427, 698, 911
FaqI	GGGAC	1 cut(s)	883
FatI	CATG	7 cut(s)	121, 164, 183, 301, 423, 694, 907
FauNDI	CATATG	1 cut(s)	238
FblI	GTMKAC	1 cut(s)	937
Fnu4HI	GCNGC	2 cut(s)	747, 925
FokI	GGATG	3 cut(s)	207, 353, 728
Fsp4HI	GCNGC	2 cut(s)	747, 925
FspBI	CTAG	2 cut(s)	489, 611
GluI	GCNGC	2 cut(s)	747, 925
GsuI	CTGGAG	2 cut(s)	185, 280
HaeIII	GGCC	1 cut(s)	299
HapII	CCGG	1 cut(s)	176
Hin1II	CATG	7 cut(s)	125, 168, 187, 305, 427, 698, 911
HincII	GTYRAC	1 cut(s)	552
HindII	GTYRAC	1 cut(s)	552
HinfI	GANTC	2 cut(s)	156, 287
HpaII	CCGG	1 cut(s)	176
HphI	GGTGA	3 cut(s)	340, 412, 831
Hpy166II	GTNNAC	4 cut(s)	228, 331, 552, 938
Hpy188I	TCNGA	4 cut(s)	214, 367, 517, 646
Hpy188III	TCNNGA	3 cut(s)	20, 202, 622
Hpy8I	GTNNAC	4 cut(s)	228, 331, 552, 938
HpyAV	CCTTC	4 cut(s)	80, 209, 384, 535
HpyCH4III	ACNGT	1 cut(s)	250
HpyCH4V	TGCA	6 cut(s)	63, 107, 164, 320, 599, 797
HpyF3I	CTNAG	1 cut(s)	467
Hsp92II	CATG	7 cut(s)	125, 168, 187, 305, 427, 698, 911
Kzo9I	GATC	1 cut(s)	16
LmnI	GCTCC	2 cut(s)	146, 365
Lsp1109I	GCAGC	2 cut(s)	733, 911
LweI	GCATC	1 cut(s)	185
MaeI	CTAG	2 cut(s)	489, 611
MaeIII	GTNAC	2 cut(s)	587, 837
MalI	GATC	1 cut(s)	18
MboI	GATC	1 cut(s)	16
MboII	GAAGA	2 cut(s)	504, 857
MluCI	AATT	9 cut(s)	108, 188, 321, 381, 434, 529, 657, 768, 903
MseI	TTAA	6 cut(s)	252, 269, 483, 528, 564, 816
MspI	CCGG	1 cut(s)	176
MspR9I	CCNGG	1 cut(s)	259
MvaI	CCWGG	1 cut(s)	259
MvnI	CGCG	1 cut(s)	84
NdeI	CATATG	1 cut(s)	238
NdeII	GATC	1 cut(s)	16
NlaIII	CATG	7 cut(s)	125, 168, 187, 305, 427, 698, 911
NlaIV	GGNNCC	4 cut(s)	30, 91, 142, 523
NmuCI	GTSAC	2 cut(s)	587, 837
PfeI	GAWTC	2 cut(s)	156, 287
PflMI	CCANNNNNTGG	1 cut(s)	908
PkrI	GCNGC	2 cut(s)	748, 926
PpuMI	RGGWCCY	1 cut(s)	728
PshBI	ATTAAT	1 cut(s)	528
Psp5II	RGGWCCY	1 cut(s)	728
Psp6I	CCWGG	1 cut(s)	257
PspEI	GGTNACC	1 cut(s)	837
PspGI	CCWGG	1 cut(s)	257
PspN4I	GGNNCC	4 cut(s)	30, 91, 142, 523
PspPI	GGNCC	3 cut(s)	298, 728, 805
PspPPI	RGGWCCY	1 cut(s)	728
RsaI	GTAC	4 cut(s)	151, 227, 472, 503
RsaNI	GTAC	4 cut(s)	150, 226, 471, 502
SaqAI	TTAA	6 cut(s)	252, 269, 483, 528, 564, 816
SatI	GCNGC	2 cut(s)	747, 925
Sau3AI	GATC	1 cut(s)	16
Sau96I	GGNCC	3 cut(s)	298, 728, 805
ScaI	AGTACT	1 cut(s)	472
ScrFI	CCNGG	1 cut(s)	259
SfaNI	GCATC	1 cut(s)	185
SfcI	CTRYAG	2 cut(s)	556, 865
SinI	GGWCC	2 cut(s)	728, 805
Sse9I	AATT	9 cut(s)	108, 188, 321, 381, 434, 529, 657, 768, 903
SsiI	CCGC	1 cut(s)	82
SspMI	CTAG	2 cut(s)	489, 611
StyD4I	CCNGG	1 cut(s)	257
TaaI	ACNGT	1 cut(s)	250
TaqI	TCGA	2 cut(s)	285, 621
TasI	AATT	9 cut(s)	108, 188, 321, 381, 434, 529, 657, 768, 903
TatI	WGTACW	3 cut(s)	225, 470, 501
TfiI	GAWTC	2 cut(s)	156, 287
Tru1I	TTAA	6 cut(s)	252, 269, 483, 528, 564, 816
Tru9I	TTAA	6 cut(s)	252, 269, 483, 528, 564, 816
TscAI	CASTG	2 cut(s)	235, 435
TseFI	GTSAC	2 cut(s)	587, 837
TseI	GCWGC	2 cut(s)	746, 924
Tsp45I	GTSAC	2 cut(s)	587, 837
TspDTI	ATGAA	3 cut(s)	318, 465, 896
TspGWI	ACGGA	2 cut(s)	149, 776
TspRI	CASTG	2 cut(s)	235, 435
Van91I	CCANNNNNTGG	1 cut(s)	908
VpaK11BI	GGWCC	2 cut(s)	728, 805
VspI	ATTAAT	1 cut(s)	528
XapI	RAATTY	3 cut(s)	188, 381, 434
XmiI	GTMKAC	1 cut(s)	937
XspI	CTAG	2 cut(s)	489, 611
ZrmI	AGTACT	1 cut(s)	472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.