Rw2G033010

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004527
Physical Location & Seq
Reverse (-)
54131817 .. 54133205
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033010.1

Sequence Viewer

Length: 1302 bp
ATGGAGTTTCATGGAGATCAAAGAAACCTCTGGAGCTTCATGGATGAAGAAAGAAAGAGCATCAAGTTACCTCGGAGACTTTCCTTTGGTAGTAACCAACAACAAAGTTCATCATCCGAAGTACCTAACATTCACTTAGCTTCTGCAGCAAGACCTTCTAATGCTGCTTCAGAACCATCAATTGATGCAATTCCTCCCAGACCTATGAGCCCCAGCACGCCATGGACACTCTCCCCGGTGCGCACTTCTCCTTCCCAACCTCTTCTTTATCATTGCATAGCCTCCCTCCACCGCCATGAAGGTAACATTTTCTCCATCACACTCTCAAAAGATTTCATATTCACCGGCTCAGAAAGTAGCCGGATTCATGCTTGGAAGCAACCAGATTGCACCGAGATAGGTTACATAAAGGCTACTTCGGGTCAAGTTCGAGCCATATTGGCCTATGGGAAAACTGCTAATTTTAGACCTAAGAAGATCACAAGCTTACCACAAAGAAATAGATTCTCTAATTTGTTTTCAAAAAAGAGCAGCCAGCAACACAATGATCACATCTCTTGCCTCGCTTACAACAAAGAAGACAAGCTGTTGTACACAGGCTCGTGGGACAAATCTGTTAAAGCTTGGAAGATAAACGAAAAGCGTTGTGTCGACTCGTTCATTGCTCATGAAGGTCATGTGAATGGGATTGTGATCAACCAGGAAGATGGGTGTGTTTTCACTTGCTCATCAGATGGCTCAGTCAAAATTTGGAGAAGGGTGTTTGGTGAAAGTTCACACATACTCACCATGACCCTGAAGTTTCAACTTTCACCAGTGAATGCCTTGGCATTGAGCTTGTCAAATAGCACCACCCTCCTCTACTCTGGCTCATCGGATGGGCTCATAAACTTCTGGGAAAAAGAGAAGATGTCTGGAAGATACAACCATGGTGGGTTTCTGCAAGGTCATCATTTCGCCGTTCTTTGCTTGGTAGCCTTGGGAGAACTAATCTTCAGTGGCTCTGAGGATGCAACCATTAGGGTTTGGAGGAGAGAGGAAGGAAATTGCTTCCATTCTTGTCTTTCTGTCATTGAGGGACATCACGGGCCGGTTAGATGCTTAGCAGTGTCTTTGGAAACAGAAAATGTGGTAAGGGTAAAGGGTTTGTTGGTTTATAGTGCAAGCTTAGACCAAACTTTTAAGGTGTGGAGGGTTAAGTCTGCAAAAGATCCACAAAGAGAGATTTTGGAGTGCGAGACAAGTCCAGTGCTGTCACCTTCATGGGTGGAGAAAAAGCTTCAGGGCAACTTTTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

433

Amino Acids

48.57

Weight (kDa)

8.95

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 109 - 263 7.4e-10 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 170 - 252 3.1e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 174 - 400 2.1e-14 WDR55
WD40_CDC20-Fz PF24807 177 - 301 2e-11 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 178 - 263 3.3e-08 TEP-1 second beta-propeller
Beta-prop_ACSF4 PF13570 178 - 351 6.4e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR5 PF25175 179 - 264 7.5e-17 WDR5 beta-propeller domain
WD40 PF00400 179 - 209 6.1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 180 - 252 2.9e-12 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 180 - 343 8.2e-12 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 181 - 305 4.8e-13 THOC3 beta-propeller domain
EIF3I PF24805 181 - 265 4.5e-07 EIF3I
WD40_Prp19 PF24814 182 - 302 1.4e-14 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 185 - 302 1e-11 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 187 - 323 1.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 215 - 251 9.3e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 264 - 403 3.7e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 278 - 403 3.3e-13 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 283 - 426 1.3e-12 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 328 - 406 7e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 242
AccI GTMKAC 1 cut(s) 651
AciI CCGC 1 cut(s) 292
AclWI GGATC 1 cut(s) 1205
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 4 cut(s) 153, 818, 979, 1265
AfaI GTAC 2 cut(s) 123, 593
AgsI TTSAA 2 cut(s) 522, 806
AjnI CCWGG 1 cut(s) 699
AluBI AGCT 8 cut(s) 36, 140, 486, 586, 623, 837, 1167, 1279
AluI AGCT 8 cut(s) 36, 140, 486, 586, 623, 837, 1167, 1279
Alw26I GTCTC 2 cut(s) 70, 1232
AlwI GGATC 1 cut(s) 1205
AoxI GGCC 2 cut(s) 441, 1088
ApeKI GCWGC 3 cut(s) 146, 164, 531
ApoI RAATTY 1 cut(s) 747
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 1088
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 5 cut(s) 334, 778, 779, 804, 1248
BanII GRGCYC 2 cut(s) 212, 885
BauI CACGAG 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 151, 158, 543
BccI CCATC 5 cut(s) 184, 323, 701, 728, 872
BceAI ACGGC 1 cut(s) 944
BciT130I CCWGG 1 cut(s) 701
BclI TGATCA 2 cut(s) 547, 693
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 70, 1232
BfmI CTRYAG 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 147, 165, 532
BlpI GCTNAGC 1 cut(s) 1102
BlsI GCNGC 3 cut(s) 148, 166, 533
Bme1390I CCNGG 2 cut(s) 236, 701
BmgT120I GGNCC 1 cut(s) 1088
BmrFI CCNGG 2 cut(s) 236, 701
BmsI GCATC 4 cut(s) 69, 175, 1000, 1088
BpiI GAAGAC 1 cut(s) 585
BpmI CTGGAG 1 cut(s) 52
Bpu1102I GCTNAGC 1 cut(s) 1102
BpuMI CCSGG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 6 cut(s) 71, 221, 234, 825, 928, 978
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bse118I RCCGGY 2 cut(s) 344, 1090
Bse1I ACTGG 3 cut(s) 815, 1247, 1297
Bse3DI GCAATG 2 cut(s) 271, 660
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 1 cut(s) 701
BseDI CCNNGG 6 cut(s) 71, 221, 234, 825, 928, 978
BseGI GGATG 4 cut(s) 49, 113, 883, 1015
BseJI GATNNNNATC 1 cut(s) 692
BseMI GCAATG 2 cut(s) 271, 660
BseMII CTCAG 3 cut(s) 363, 753, 996
BseNI ACTGG 3 cut(s) 815, 1247, 1297
BseRI GAGGAG 2 cut(s) 848, 1045
BseXI GCAGC 3 cut(s) 151, 158, 543
BseYI CCCAGC 1 cut(s) 212
BshFI GGCC 2 cut(s) 443, 1090
BsiSI CCGG 4 cut(s) 236, 345, 361, 1091
BslFI GGGAC 2 cut(s) 620, 1092
BsmAI GTCTC 2 cut(s) 70, 1232
BsmFI GGGAC 2 cut(s) 620, 1092
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 2 cut(s) 443, 1090
Bsp1286I GDGCHC 2 cut(s) 212, 885
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 5 cut(s) 16, 477, 547, 693, 1210
Bsp1720I GCTNAGC 1 cut(s) 1102
Bsp19I CCATGG 2 cut(s) 221, 928
BspACI CCGC 1 cut(s) 292
BspANI GGCC 2 cut(s) 443, 1090
BspCNI CTCAG 3 cut(s) 362, 752, 997
BspHI TCATGA 1 cut(s) 667
BspMAI CTGCAG 1 cut(s) 148
BspPI GGATC 1 cut(s) 1205
BsrDI GCAATG 2 cut(s) 271, 660
BsrFI RCCGGY 2 cut(s) 344, 1090
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 3 cut(s) 815, 1247, 1297
BssAI RCCGGY 2 cut(s) 344, 1090
BssECI CCNNGG 6 cut(s) 71, 221, 234, 825, 928, 978
BssMI GATC 5 cut(s) 16, 477, 547, 693, 1210
BssSI CACGAG 1 cut(s) 601
BssT1I CCWWGG 4 cut(s) 221, 825, 928, 978
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 1 cut(s) 701
Bst6I CTCTTC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 591
BstC8I GCNNGC 3 cut(s) 218, 536, 1165
BstDEI CTNAG 7 cut(s) 136, 349, 471, 739, 1005, 1102, 1168
BstDSI CCRYGG 2 cut(s) 221, 928
BstF5I GGATG 4 cut(s) 49, 113, 883, 1015
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 5 cut(s) 19, 480, 550, 696, 1213
BstMAI GTCTC 2 cut(s) 70, 1232
BstMBI GATC 5 cut(s) 16, 477, 547, 693, 1210
BstMWI GCNNNNNNNGC 2 cut(s) 146, 440
BstNI CCWGG 1 cut(s) 701
BstSCI CCNGG 2 cut(s) 234, 699
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 151, 158, 543
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 1210
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 1 cut(s) 1210
BsuRI GGCC 2 cut(s) 443, 1090
BtgI CCRYGG 2 cut(s) 221, 928
BtsCI GGATG 4 cut(s) 49, 113, 883, 1015
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 4 cut(s) 822, 1003, 1113, 1254
Cac8I GCNNGC 3 cut(s) 218, 536, 1165
CciI TCATGA 1 cut(s) 667
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 2 cut(s) 344, 1090
Cfr13I GGNCC 1 cut(s) 1088
Csp6I GTAC 2 cut(s) 122, 592
CspCI CAANNNNNGTGG 2 cut(s) 913, 948
CviQI GTAC 2 cut(s) 122, 592
DdeI CTNAG 7 cut(s) 136, 349, 471, 739, 1005, 1102, 1168
DpnI GATC 5 cut(s) 18, 479, 549, 695, 1212
DpnII GATC 5 cut(s) 16, 477, 547, 693, 1210
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco130I CCWWGG 4 cut(s) 221, 825, 928, 978
Eco24I GRGCYC 2 cut(s) 212, 885
Eco57I CTGAAG 4 cut(s) 153, 818, 979, 1265
EcoRII CCWGG 1 cut(s) 699
EcoT14I CCWWGG 4 cut(s) 221, 825, 928, 978
EcoT38I GRGCYC 2 cut(s) 212, 885
ErhI CCWWGG 4 cut(s) 221, 825, 928, 978
FaqI GGGAC 2 cut(s) 620, 1092
FbaI TGATCA 2 cut(s) 547, 693
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 3 cut(s) 147, 165, 532
FokI GGATG 4 cut(s) 56, 100, 890, 1022
FriOI GRGCYC 2 cut(s) 212, 885
Fsp4HI GCNGC 3 cut(s) 147, 165, 532
FspAI RTGCGCAY 1 cut(s) 242
FspI TGCGCA 1 cut(s) 242
GlaI GCGC 1 cut(s) 242
GluI GCNGC 3 cut(s) 147, 165, 532
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 52
HaeIII GGCC 2 cut(s) 443, 1090
HapII CCGG 4 cut(s) 236, 345, 361, 1091
HhaI GCGC 1 cut(s) 243
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 4 cut(s) 484, 621, 1165, 1277
HinfI GANTC 3 cut(s) 364, 504, 653
HpaII CCGG 4 cut(s) 236, 345, 361, 1091
HphI GGTGA 5 cut(s) 334, 778, 779, 804, 1248
Hpy166II GTNNAC 3 cut(s) 594, 652, 776
Hpy188I TCNGA 7 cut(s) 75, 118, 172, 352, 733, 877, 1006
Hpy188III TCNNGA 3 cut(s) 31, 668, 915
Hpy8I GTNNAC 3 cut(s) 594, 652, 776
HpyAV CCTTC 7 cut(s) 165, 261, 293, 665, 750, 1034, 1269
HpyCH4V TGCA 8 cut(s) 146, 188, 276, 390, 943, 1013, 1163, 1205
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 440
HpyF3I CTNAG 7 cut(s) 136, 349, 471, 739, 1005, 1102, 1168
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 547, 693
Kzo9I GATC 5 cut(s) 16, 477, 547, 693, 1210
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 3 cut(s) 151, 158, 543
LweI GCATC 4 cut(s) 69, 175, 1000, 1088
MaeIII GTNAC 5 cut(s) 66, 92, 302, 401, 1254
MalI GATC 5 cut(s) 18, 479, 549, 695, 1212
MboI GATC 5 cut(s) 16, 477, 547, 693, 1210
MboII GAAGA 9 cut(s) 59, 254, 487, 590, 640, 716, 919, 930, 985
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 1 cut(s) 1210
MhlI GDGCHC 2 cut(s) 212, 885
MluCI AATT 6 cut(s) 180, 189, 460, 511, 747, 1045
MlyI GAGTC 1 cut(s) 647
MseI TTAA 3 cut(s) 618, 1182, 1197
MslI CAYNNNNRTG 3 cut(s) 294, 681, 1261
MspI CCGG 4 cut(s) 236, 345, 361, 1091
MspR9I CCNGG 2 cut(s) 236, 701
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 146, 440
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 2 cut(s) 221, 928
NdeII GATC 5 cut(s) 16, 477, 547, 693, 1210
NmuCI GTSAC 1 cut(s) 1254
NsbI TGCGCA 1 cut(s) 242
PagI TCATGA 1 cut(s) 667
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 2 cut(s) 364, 504
PkrI GCNGC 3 cut(s) 148, 166, 533
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
Psp6I CCWGG 1 cut(s) 699
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 1 cut(s) 699
PspPI GGNCC 1 cut(s) 1088
PstI CTGCAG 1 cut(s) 148
PsuI RGATCY 1 cut(s) 1210
RsaI GTAC 2 cut(s) 123, 593
RsaNI GTAC 2 cut(s) 122, 592
RseI CAYNNNNRTG 3 cut(s) 294, 681, 1261
SalI GTCGAC 1 cut(s) 650
SaqAI TTAA 3 cut(s) 618, 1182, 1197
SatI GCNGC 3 cut(s) 147, 165, 532
Sau3AI GATC 5 cut(s) 16, 477, 547, 693, 1210
Sau96I GGNCC 1 cut(s) 1088
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 2 cut(s) 236, 701
SduI GDGCHC 2 cut(s) 212, 885
SfaNI GCATC 4 cut(s) 69, 175, 1000, 1088
SfcI CTRYAG 1 cut(s) 144
SmiMI CAYNNNNRTG 3 cut(s) 294, 681, 1261
Sse9I AATT 6 cut(s) 180, 189, 460, 511, 747, 1045
SsiI CCGC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 234, 699
StyI CCWWGG 4 cut(s) 221, 825, 928, 978
TaqI TCGA 2 cut(s) 430, 651
TasI AATT 6 cut(s) 180, 189, 460, 511, 747, 1045
TatI WGTACW 1 cut(s) 591
TfiI GAWTC 2 cut(s) 364, 504
Tru1I TTAA 3 cut(s) 618, 1182, 1197
Tru9I TTAA 3 cut(s) 618, 1182, 1197
TscAI CASTG 4 cut(s) 822, 1003, 1113, 1254
TseFI GTSAC 1 cut(s) 1254
TseI GCWGC 3 cut(s) 146, 164, 531
Tsp45I GTSAC 1 cut(s) 1254
TspDTI ATGAA 9 cut(s) 28, 60, 99, 312, 325, 356, 649, 684, 1251
TspRI CASTG 4 cut(s) 822, 1003, 1113, 1254
XapI RAATTY 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.