MD09G1207500.v1.1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004527
Physical Location & Seq
Forward (+)
19878495 .. 19879877
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1207500.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGAGTTTCATGGCGAAAGAAATCTATGGAGCTTCATGGACGAAGAGCGAAAGAGCATCAAGTTACCTCGGAGACTTTCTTTTGGTGATGGTGACCAGCAACAAAGTTCATCAGAACCCGAAAACATTCGTTCGGCTTCTGCAGCAAGACCAGACGTAGCTGCGTCCGAACCAACAATACCTCCGTGGCCTATGAGCCCTGGGACACCGTGGACACTCTCCCCGGTGCGCACCTCTCCCTCCAAATCCCTTCTTTACCACTGCATTGCCTCCCTTCACCGCCATGAAGGCAACATTTTCTCGATCACATTGTCAAGAGATTTTATATTCACGAGCTCGGAGAGTAGCCGGATTCATGTTTGGAAGCAACCGGATTGCACTGAAATCGGTTGCATAAAGGCTACCTCAGGTCAAATTCGAGCTATTTTGGCCTCCGGGAAAATCTTATTCACCGCACACGGCGACTGCAAAATTCGCATATGGAATGCGTCGAATGCAGAGAATTTTCGACCGAAGAAGATCACAACGCTTCCTCAAAGAGGCCGGTTTTCGCTGTTTTCGAAAAAGAGCAGCCAGCAGCACAACGATCGCATCTCTTGCCTAGCTTACAACAAGGAAGACAAGCTCTTGTACACAGGCTCATGGGACAAAACAGTCAAGGCATGGAAGATCAACGAAAGGCGCTGCGTGGATTCGTTTGTAGCTCACGAAGGCCACATAAATGGAATCGCGATCAACCAAGAAGACGGCTGCGTGTTCACTTGCTCCTCGGACGGCTCAGTCAAGATTTGGAGAAGGGTGTTCGGTGAAAGTTCTCACATCCTAACCATGACTCTCAAGTTCCAACTTTCGCCGGTGAACGCCTTGGCGTTGAGCTTGTCATCCACGACCACCCTCCTCTACTCCGGCTCGTCAGACGGGCTAATAAACTTTTGGGAAAAGGAGAAGATGTCCGGGAGGTACAATCACGGAGGGTTTCTACAAGGTCATCATTTCGCCGTCCTTTGCTTGGTAGCGTTAGGAGAACTAGTTTTCAGTGGCTCCGAGGACGCAACGATTAGGGTTTGGAGAAGAGAGGAAGGGAACTGCTTCCACTCATGTCTCTCCGTGATAGAAGGGCATCACGGACCGGTGAGATGCTTGGCAGTGGCCTTGGAGACGGAGAATTTGGTAAGGGTGAAAGGTTTGTTGGTTTACAGTGCAAGTTTGGATCAAACTTTTAAGGTTTGGAGGGTTAAAGTTTTTCCTGCTGAGAAGGTACACTTGGTGGATGAAACTGCTGCTGCCAAAGACCCACAGAGAGAGATTGTGGAGTGTGAGACAAGCCCTGTGTTGTCACCTTCATGGGTGGAGAAAAAGCTTCAAGGCAACTATTTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

461

Amino Acids

51.6

Weight (kDa)

8.61

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 92 - 277 4.4e-22 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 104 - 266 5.7e-11 TEP-1 second beta-propeller
Beta-prop_IP5PC_F PF23754 139 - 265 1.9e-06 IP5P C-F beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 145 - 265 5.2e-09 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 188 - 414 5e-14 WDR55
Beta-prop_WDR3_2nd PF25172 188 - 266 4.1e-09 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 190 - 315 2.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_ACSF4 PF13570 191 - 357 6.4e-06 Beta-alanine-activating enzyme, beta-propeller
WD40 PF00400 192 - 222 2.3e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 193 - 356 3e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 193 - 265 9.2e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 194 - 317 2.9e-13 THOC3 beta-propeller domain
EIF3I PF24805 194 - 276 4.5e-07 EIF3I
WD40_Prp19 PF24814 195 - 315 9.4e-15 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 197 - 276 3e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 200 - 359 4.7e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 228 - 264 3.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 278 - 413 2.5e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 278 - 414 1.2e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 290 - 365 3.6e-06 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 298 - 411 3e-10 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 341 - 413 5.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 653, 779
Acc16I TGCGCA 1 cut(s) 230
AccII CGCG 1 cut(s) 731
AciI CCGC 2 cut(s) 280, 453
AclWI GGATC 1 cut(s) 1218
AcsI RAATTY 4 cut(s) 414, 471, 502, 1165
AdeI CACNNNGTG 1 cut(s) 1267
AfaI GTAC 3 cut(s) 632, 962, 1260
AfiI CCNNNNNNNGG 2 cut(s) 539, 1009
AgeI ACCGGT 1 cut(s) 1129
AgsI TTSAA 1 cut(s) 1364
AhlI ACTAGT 1 cut(s) 1027
AjnI CCWGG 1 cut(s) 199
AjuI GAANNNNNNNTTGG 4 cut(s) 1172, 1204, 1247, 1279
AluBI AGCT 9 cut(s) 33, 161, 336, 422, 605, 625, 704, 876, 1360
AluI AGCT 9 cut(s) 33, 161, 336, 422, 605, 625, 704, 876, 1360
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 4 cut(s) 67, 1106, 1151, 1313
AlwI GGATC 1 cut(s) 1218
AoxI GGCC 5 cut(s) 188, 429, 541, 712, 1149
ApeKI GCWGC 8 cut(s) 143, 161, 570, 577, 684, 750, 1280, 1283
ApoI RAATTY 4 cut(s) 414, 471, 502, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 1150, 1182
AsiGI ACCGGT 1 cut(s) 1129
Asp700I GAANNNNTTC 2 cut(s) 126, 1088
AspLEI GCGC 2 cut(s) 231, 684
AspS9I GGNCC 1 cut(s) 1127
AsuC2I CCSGG 3 cut(s) 224, 436, 955
AsuHPI GGTGA 9 cut(s) 98, 104, 269, 442, 818, 868, 1144, 1189, 1329
AsuII TTCGAA 1 cut(s) 560
AvaII GGWCC 1 cut(s) 1127
AxyI CCTNAGG 1 cut(s) 406
BanII GRGCYC 2 cut(s) 200, 338
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 624, 750
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 8 cut(s) 148, 155, 582, 589, 671, 737, 1267, 1270
BccI CCATC 1 cut(s) 83
BceAI ACGGC 4 cut(s) 475, 763, 790, 983
BcgI CGANNNNNNTGC 4 cut(s) 367, 401, 569, 603
BciT130I CCWGG 1 cut(s) 201
BcnI CCSGG 3 cut(s) 224, 436, 955
BcoDI GTCTC 4 cut(s) 67, 1106, 1151, 1313
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 2 cut(s) 602, 1028
BfmI CTRYAG 1 cut(s) 141
BfoI RGCGCY 1 cut(s) 685
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 8 cut(s) 144, 162, 571, 578, 685, 751, 1281, 1284
BlsI GCNGC 8 cut(s) 145, 163, 572, 579, 686, 752, 1282, 1285
Bme1390I CCNGG 4 cut(s) 201, 224, 436, 955
Bme18I GGWCC 1 cut(s) 1127
BmgT120I GGNCC 1 cut(s) 1127
BmiI GGNNCC 1 cut(s) 1042
BmrFI CCNGG 4 cut(s) 201, 224, 436, 955
BmsI GCATC 4 cut(s) 66, 600, 1127, 1129
BpiI GAAGAC 2 cut(s) 624, 750
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 821
BpuMI CCSGG 3 cut(s) 224, 436, 955
BsaWI WCCGGW 2 cut(s) 370, 1129
BsaXI ACNNNNNCTCC 6 cut(s) 166, 196, 935, 965, 1014, 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 1009
Bse118I RCCGGY 3 cut(s) 543, 853, 1129
Bse21I CCTNAGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 1 cut(s) 201
BseGI GGATG 3 cut(s) 819, 881, 1276
BseLI CCNNNNNNNGG 2 cut(s) 539, 1009
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 3 cut(s) 420, 792, 1242
BseRI GAGGAG 2 cut(s) 757, 887
BseXI GCAGC 8 cut(s) 148, 155, 582, 589, 671, 737, 1267, 1270
Bsh1236I CGCG 1 cut(s) 731
Bsh1285I CGRYCG 2 cut(s) 512, 589
BshFI GGCC 5 cut(s) 190, 431, 543, 714, 1151
BshTI ACCGGT 1 cut(s) 1129
BsiEI CGRYCG 2 cut(s) 512, 589
BsiHKAI GWGCWC 1 cut(s) 338
BsiSI CCGG 9 cut(s) 224, 349, 371, 435, 544, 854, 906, 954, 1130
BslFI GGGAC 2 cut(s) 217, 659
BslI CCNNNNNNNGG 2 cut(s) 539, 1009
BsmAI GTCTC 4 cut(s) 67, 1106, 1151, 1313
BsmBI CGTCTC 1 cut(s) 1151
BsmFI GGGAC 2 cut(s) 217, 659
BsmI GAATGC 2 cut(s) 490, 499
BsnI GGCC 5 cut(s) 190, 431, 543, 714, 1151
Bsp119I TTCGAA 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 200, 338
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
Bsp68I TCGCGA 1 cut(s) 731
BspACI CCGC 2 cut(s) 280, 453
BspANI GGCC 5 cut(s) 190, 431, 543, 714, 1151
BspCNI CTCAG 3 cut(s) 419, 791, 1243
BspFNI CGCG 1 cut(s) 731
BspLI GGNNCC 1 cut(s) 1042
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 1 cut(s) 1218
BspQI GCTCTTC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 3 cut(s) 543, 853, 1129
BsrGI TGTACA 1 cut(s) 630
BssAI RCCGGY 3 cut(s) 543, 853, 1129
BssMI GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
BssSI CACGAG 1 cut(s) 331
BssT1I CCWWGG 2 cut(s) 864, 1152
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 3 cut(s) 210, 655, 1199
Bst6I CTCTTC 2 cut(s) 39, 1066
BstAPI GCANNNNNTGC 1 cut(s) 597
BstAUI TGTACA 1 cut(s) 630
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 3 cut(s) 406, 778, 1251
BstDSI CCRYGG 2 cut(s) 185, 209
BstEII GGTNACC 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 537
BstF5I GGATG 3 cut(s) 819, 881, 1276
BstFNI CGCG 1 cut(s) 731
BstH2I RGCGCY 1 cut(s) 685
BstHHI GCGC 2 cut(s) 231, 684
BstKTI GATC 6 cut(s) 306, 522, 589, 672, 735, 1213
BstMAI GTCTC 4 cut(s) 67, 1106, 1151, 1313
BstMBI GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
BstMCI CGRYCG 2 cut(s) 512, 589
BstMWI GCNNNNNNNGC 6 cut(s) 143, 288, 428, 474, 494, 597
BstNI CCWGG 1 cut(s) 201
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 4 cut(s) 199, 222, 434, 953
BstSFI CTRYAG 1 cut(s) 141
BstUI CGCG 1 cut(s) 731
BstV1I GCAGC 8 cut(s) 148, 155, 582, 589, 671, 737, 1267, 1270
BstV2I GAAGAC 2 cut(s) 624, 750
BstXI CCANNNNNNTGG 1 cut(s) 722
Bsu36I CCTNAGG 1 cut(s) 406
BsuRI GGCC 5 cut(s) 190, 431, 543, 714, 1151
BtgI CCRYGG 2 cut(s) 185, 209
BtsCI GGATG 3 cut(s) 819, 881, 1276
BtsI GCAGTG 2 cut(s) 259, 1152
BtsIMutI CAGTG 5 cut(s) 259, 378, 1042, 1152, 1204
BtuMI TCGCGA 1 cut(s) 731
Cac8I GCNNGC 1 cut(s) 575
CfoI GCGC 2 cut(s) 231, 684
Cfr10I RCCGGY 3 cut(s) 543, 853, 1129
Cfr13I GGNCC 1 cut(s) 1127
CpoI CGGWCCG 1 cut(s) 1127
CseI GACGC 3 cut(s) 153, 477, 1058
Csp6I GTAC 3 cut(s) 631, 961, 1259
CspAI ACCGGT 1 cut(s) 1129
CspI CGGWCCG 1 cut(s) 1127
CviAII CATG 9 cut(s) 11, 37, 284, 356, 642, 663, 829, 1098, 1344
CviQI GTAC 3 cut(s) 631, 961, 1259
DdeI CTNAG 3 cut(s) 406, 778, 1251
DpnI GATC 6 cut(s) 305, 521, 588, 671, 734, 1212
DpnII GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
DraIII CACNNNGTG 1 cut(s) 1267
DrdI GACNNNNNNGTC 2 cut(s) 653, 779
DseDI GACNNNNNNGTC 2 cut(s) 653, 779
Eam1104I CTCTTC 2 cut(s) 39, 1066
EarI CTCTTC 2 cut(s) 39, 1066
Ecl136II GAGCTC 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 864, 1152
Eco24I GRGCYC 2 cut(s) 200, 338
Eco47I GGWCC 1 cut(s) 1127
Eco53kI GAGCTC 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 406
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 336
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 864, 1152
EcoT38I GRGCYC 2 cut(s) 200, 338
ErhI CCWWGG 2 cut(s) 864, 1152
Esp3I CGTCTC 1 cut(s) 1151
FaeI CATG 9 cut(s) 14, 40, 287, 359, 645, 666, 832, 1101, 1347
FalI AAGNNNNNCTT 2 cut(s) 1247, 1279
FaqI GGGAC 2 cut(s) 217, 659
FatI CATG 9 cut(s) 10, 36, 283, 355, 641, 662, 828, 1097, 1343
FauNDI CATATG 1 cut(s) 479
Fnu4HI GCNGC 8 cut(s) 144, 162, 571, 578, 685, 751, 1281, 1284
FokI GGATG 3 cut(s) 806, 868, 1283
FriOI GRGCYC 2 cut(s) 200, 338
Fsp4HI GCNGC 8 cut(s) 144, 162, 571, 578, 685, 751, 1281, 1284
FspAI RTGCGCAY 1 cut(s) 230
FspBI CTAG 2 cut(s) 602, 1028
FspI TGCGCA 1 cut(s) 230
GlaI GCGC 2 cut(s) 230, 683
GluI GCNGC 8 cut(s) 144, 162, 571, 578, 685, 751, 1281, 1284
HaeII RGCGCY 1 cut(s) 685
HaeIII GGCC 5 cut(s) 190, 431, 543, 714, 1151
HapII CCGG 9 cut(s) 224, 349, 371, 435, 544, 854, 906, 954, 1130
HgaI GACGC 3 cut(s) 153, 477, 1058
HhaI GCGC 2 cut(s) 231, 684
Hin1II CATG 9 cut(s) 14, 40, 287, 359, 645, 666, 832, 1101, 1347
Hin6I GCGC 2 cut(s) 229, 682
HinP1I GCGC 2 cut(s) 229, 682
HindIII AAGCTT 1 cut(s) 1358
HinfI GANTC 4 cut(s) 352, 692, 726, 832
HpaII CCGG 9 cut(s) 224, 349, 371, 435, 544, 854, 906, 954, 1130
HphI GGTGA 9 cut(s) 98, 104, 269, 442, 818, 868, 1144, 1189, 1329
Hpy166II GTNNAC 6 cut(s) 213, 633, 759, 859, 1195, 1261
Hpy188I TCNGA 7 cut(s) 72, 115, 169, 340, 772, 916, 1045
Hpy188III TCNNGA 6 cut(s) 301, 315, 331, 707, 730, 784
Hpy8I GTNNAC 6 cut(s) 213, 633, 759, 859, 1195, 1261
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 9 cut(s) 260, 281, 284, 704, 789, 1073, 1109, 1249, 1350
HpyCH4III ACNGT 3 cut(s) 210, 655, 1199
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 7 cut(s) 143, 264, 378, 393, 468, 497, 1202
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 288, 428, 474, 494, 597
HpyF3I CTNAG 3 cut(s) 406, 778, 1251
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 9 cut(s) 14, 40, 287, 359, 645, 666, 832, 1101, 1347
HspAI GCGC 2 cut(s) 229, 682
Kzo9I GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 30, 770, 1046
Lsp1109I GCAGC 8 cut(s) 148, 155, 582, 589, 671, 737, 1267, 1270
LweI GCATC 4 cut(s) 66, 600, 1127, 1129
MaeI CTAG 2 cut(s) 602, 1028
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 3 cut(s) 63, 92, 1335
MalI GATC 6 cut(s) 305, 521, 588, 671, 734, 1212
MboI GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
MboII GAAGA 8 cut(s) 56, 526, 529, 629, 679, 755, 958, 1083
MhlI GDGCHC 2 cut(s) 200, 338
MluCI AATT 4 cut(s) 414, 471, 502, 1165
MlyI GAGTC 1 cut(s) 826
MmeI TCCRAC 1 cut(s) 868
MroXI GAANNNNTTC 2 cut(s) 126, 1088
MseI TTAA 2 cut(s) 1221, 1236
MslI CAYNNNNRTG 3 cut(s) 282, 720, 1342
MspI CCGG 9 cut(s) 224, 349, 371, 435, 544, 854, 906, 954, 1130
MspR9I CCNGG 4 cut(s) 201, 224, 436, 955
Mva1269I GAATGC 2 cut(s) 490, 499
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 731
MwoI GCNNNNNNNGC 6 cut(s) 143, 288, 428, 474, 494, 597
NciI CCSGG 3 cut(s) 224, 436, 955
NdeI CATATG 1 cut(s) 479
NdeII GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
NlaIII CATG 9 cut(s) 14, 40, 287, 359, 645, 666, 832, 1101, 1347
NlaIV GGNNCC 1 cut(s) 1042
NmuCI GTSAC 2 cut(s) 92, 1335
NruI TCGCGA 1 cut(s) 731
NsbI TGCGCA 1 cut(s) 230
NspV TTCGAA 1 cut(s) 560
PasI CCCWGGG 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 557
PctI GAATGC 2 cut(s) 490, 499
PdmI GAANNNNTTC 2 cut(s) 126, 1088
PfeI GAWTC 3 cut(s) 352, 692, 726
PfoI TCCNGGA 2 cut(s) 434, 953
PinAI ACCGGT 1 cut(s) 1129
PkrI GCNGC 8 cut(s) 145, 163, 572, 579, 686, 752, 1282, 1285
Ple19I CGATCG 1 cut(s) 589
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 199
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 1042
PspPI GGNCC 1 cut(s) 1127
PsrI GAACNNNNNNTAC 2 cut(s) 162, 194
PstI CTGCAG 1 cut(s) 145
PvuI CGATCG 1 cut(s) 589
RruI TCGCGA 1 cut(s) 731
RsaI GTAC 3 cut(s) 632, 962, 1260
RsaNI GTAC 3 cut(s) 631, 961, 1259
RseI CAYNNNNRTG 3 cut(s) 282, 720, 1342
Rsr2I CGGWCCG 1 cut(s) 1127
RsrII CGGWCCG 1 cut(s) 1127
SacI GAGCTC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 1221, 1236
SatI GCNGC 8 cut(s) 144, 162, 571, 578, 685, 751, 1281, 1284
Sau3AI GATC 6 cut(s) 303, 519, 586, 669, 732, 1210
Sau96I GGNCC 1 cut(s) 1127
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 4 cut(s) 201, 224, 436, 955
SduI GDGCHC 2 cut(s) 200, 338
SfaNI GCATC 4 cut(s) 66, 600, 1127, 1129
SfcI CTRYAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 560
SgrAI CRCCGGYG 1 cut(s) 853
SinI GGWCC 1 cut(s) 1127
SmiMI CAYNNNNRTG 3 cut(s) 282, 720, 1342
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
SpeI ACTAGT 1 cut(s) 1027
Sse9I AATT 4 cut(s) 414, 471, 502, 1165
SsiI CCGC 2 cut(s) 280, 453
SspMI CTAG 2 cut(s) 602, 1028
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 4 cut(s) 199, 222, 434, 953
StyI CCWWGG 2 cut(s) 864, 1152
TaaI ACNGT 3 cut(s) 210, 655, 1199
TaiI ACGT 1 cut(s) 159
TaqI TCGA 5 cut(s) 302, 418, 491, 508, 560
TaqII GACCGA 1 cut(s) 526
TasI AATT 4 cut(s) 414, 471, 502, 1165
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 3 cut(s) 352, 692, 726
Tru1I TTAA 2 cut(s) 1221, 1236
Tru9I TTAA 2 cut(s) 1221, 1236
TscAI CASTG 5 cut(s) 266, 385, 1042, 1152, 1204
TseFI GTSAC 2 cut(s) 92, 1335
TseI GCWGC 8 cut(s) 143, 161, 570, 577, 684, 750, 1280, 1283
Tsp45I GTSAC 2 cut(s) 92, 1335
TspDTI ATGAA 6 cut(s) 25, 99, 300, 344, 1287, 1332
TspGWI ACGGA 5 cut(s) 174, 984, 1096, 1140, 1175
TspRI CASTG 5 cut(s) 266, 385, 1042, 1152, 1204
VpaK11BI GGWCC 1 cut(s) 1127
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 4 cut(s) 414, 471, 502, 1165
XmnI GAANNNNTTC 2 cut(s) 126, 1088
XspI CTAG 2 cut(s) 602, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.