Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0004527

Physical Location & Seq

Genomic Coordinates Forward (+)

4701956 .. 4703742

Gene Structure 1787 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00162050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGACCACATTAAGTAGAGTTCTACCTCATCGAATTTTCCCAACAACAGTCGCTGCAGCTACAACATATGTAGCAATCAAAACACACCAGCAAAAATTGCTGACTATTAGTATGTCTGCCTACTCTAGTCCCCGTGGACCCTGTCCCCTCACCAAACCCCATCCCCCTTCACTCCTTTACCACTGCATTGCCTCCCTCTATCGCCGCGAAGGCTCCATCTCCTCCATTGCAGTCTCAAAGCAGCAAGGAGTAGTCTTCACTGGCTCAGAGAGTACCCGAGTTCGTGTGTGGAGACAACCAGACCACATTGAATACGGTTGTCTCAATGCGACTTCAGGTGAAGTACGAGCTATTGCAACTCATGGTAATATGCTTTTCACCTCACACAAAGACCACAAAATCCGAGTGTGGAACTTCTCGGTCACAGATCACAATTTCAAGTCCAAAAAGGTTTCTTCTCTCCCCAAAACTAGCTCTTTCCCTTTATTTTCAAGATTTACAAACACCAAACAGCAACACAAAGAATGCATTTCCTGCTTGGCATATTATCAAACAGATGGTCTGTTGTATACTGGCTCCCATGACACAACAGTCAAAGCATGGCGGCTTATAACCAATCACTGTGTGGACTCATTTGTGGCACATGAAGACAATGTGAATGCAATGGTGGTGAACCAAGATGATGGGTGCGTTTTCACTTGCTCCTCAGATGCCTTAAACACATCATCCAACTCTTGCTTCCTCTACTCTGGTTCTTCAGACGGAACCATTAGTTTCTGGGAGAAGGAGAAACTGACCCAAAGGTTTAACCATGGTGGGTTTTTACAGGGCCACCATTTTTCAGTTCTGTGTTTAGTGGCCATCGAGAAGTTGATTTTCAGTGGATCAGAGGATACTACAATTAGGGTTTGGAGGAGGGAAGAAGGGGGTTGTTTTCATGAATGTTTGGCTGTGTTGGAGGGGCATAGAGGCCCTGTGAGGTGTTTGGCTGCTTGTTTGGAGATTGAGAAAGTTGTGAAGGGTTTTTTGGTTTATAGTGGCAGTTTGGATCAAACTTTTAAGGTGTGGAGTGTTAAGGTCTCGCCGCCGGAAGAGGGAATGGGGTTGAACTGTTATATGGATCGAAGATTTTCGAGGAACAATGGGATGCAATGTGAGACCAGGACTGTGTTGTCTCCTTCATGGGTTGAGAGGAAGCTGCTCCAGAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005874 GO:0005875 GO:0005938 GO:0007154 GO:0007165 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0017016 GO:0017048 GO:0019899 GO:0019932 GO:0019933 GO:0019935 GO:0023052 GO:0031267 GO:0031592 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035556 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044380 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0044877 GO:0045504 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051179 GO:0051640 GO:0051641 GO:0051642 GO:0051645 GO:0051716 GO:0061842 GO:0065007 GO:0070727 GO:0070840 GO:0071539 GO:0071944 GO:0072698 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1905508

KEGG Pathways

Metabolic & Signaling

ko04114 ko04120 ko04218 ko04310 ko04340 ko04341 ko04390 ko04624 ko04710 ko05131 map04114 map04120 map04218 map04310 map04340 map04341 map04390 map04624 map04710 map05131

Pfam Domains

Protein Families

WD40

Protein Analysis

403

Amino Acids

45.25

Weight (kDa)

8.94

Isoelectric Point (pI)

44.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_WDHD1_1st	PF24817	66 - 162	1.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	69 - 239	5.6e-17	WDR5 beta-propeller domain
EIF3I	PF24805	69 - 238	5.2e-07	EIF3I
WD40_Prp19	PF24814	167 - 239	3.5e-07	Prp19 WD40 domain
WDR55	PF24796	167 - 239	3.5e-07	WDR55
Beta-prop_THOC3	PF25174	168 - 237	9.8e-06	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	170 - 359	1.3e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	171 - 238	5.3e-12	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	173 - 308	6e-11	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	239 - 359	5.9e-12	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	240 - 314	1.9e-06	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	241 - 359	4.4e-13	WDR5 beta-propeller domain
WD40_Prp19	PF24814	243 - 331	6.3e-07	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	246 - 362	9.4e-11	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004527)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35690 FvH4_6g31961
malus_domestica	MD08G1115200.v1.1 MD09G1207500.v1.1 MD17G1188800.v1.1
prunus_persica	Prupe.1G449400_v2.0.a1 Prupe.3G063300_v2.0.a1
pyrus_communis	pycom09g12370
rosa_chinensis	RchiOBHm_Chr2g0139471 RchiOBHm_Chr6g0306111
rosa_laevigata	RLG00000010824 RLG00000019813
rosa_multiflora	Rmu_sc0000785.1_g000023 Rmu_sc0000903.1_g000022 Rmu_sc0001983.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105410 Rroxscaffold_7G00162050
rosa_rugosa	Rorug06G0282100 Rorug06G0346800
rosa_samantha	Rh2AG407500 Rh2BG418100 Rh2CG393500 Rh2DG426900 Rh6AG459200 Rh6BG441700 Rh6CG473700 Rh6DG459900
rosa_wichuraiana	Rw2G033010 Rw2G033270 Rw6G006540 Rw6G040030 Rw7G016590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	611
AasI	GACNNNNNNGTC	2 cut(s)	590, 1171
AccI	GTMKAC	1 cut(s)	569
AccII	CGCG	1 cut(s)	207
AciI	CCGC	3 cut(s)	205, 604, 1085
AclWI	GGATC	3 cut(s)	892, 1056, 1128
AcoI	YGGCCR	1 cut(s)	858
AcsI	RAATTY	1 cut(s)	33
AcuI	CTGAAG	2 cut(s)	318, 741
AdeI	CACNNNGTG	1 cut(s)	625
AfaI	GTAC	2 cut(s)	274, 345
AfiI	CCNNNNNNNGG	1 cut(s)	1094
AgsI	TTSAA	4 cut(s)	311, 439, 492, 1108
AjnI	CCWGG	1 cut(s)	1160
AjuI	GAANNNNNNNTTGG	4 cut(s)	722, 754, 1010, 1042
AluBI	AGCT	4 cut(s)	59, 350, 474, 1198
AluI	AGCT	4 cut(s)	59, 350, 474, 1198
Alw26I	GTCTC	6 cut(s)	238, 286, 326, 1084, 1151, 1179
AlwI	GGATC	3 cut(s)	892, 1056, 1128
AlwNI	CAGNNNCTG	1 cut(s)	53
Ama87I	CYCGRG	1 cut(s)	276
AoxI	GGCC	3 cut(s)	829, 858, 970
ApeKI	GCWGC	5 cut(s)	53, 56, 241, 989, 1198
ApoI	RAATTY	1 cut(s)	33
ArsI	GACNNNNNNTTYG	2 cut(s)	544, 576
Asp700I	GAANNNNTTC	1 cut(s)	1129
AspS9I	GGNCC	3 cut(s)	137, 829, 971
AsuHPI	GGTGA	4 cut(s)	142, 350, 370, 682
AvaI	CYCGRG	1 cut(s)	276
AvaII	GGWCC	1 cut(s)	137
BalI	TGGCCA	1 cut(s)	860
BbsI	GAAGAC	2 cut(s)	247, 654
BbvI	GCAGC	5 cut(s)	40, 68, 253, 976, 1185
BccI	CCATC	5 cut(s)	168, 224, 551, 677, 869
BciT130I	CCWGG	1 cut(s)	1162
BciVI	GTATCC	1 cut(s)	886
BcoDI	GTCTC	6 cut(s)	238, 286, 326, 1084, 1151, 1179
BfaI	CTAG	2 cut(s)	126, 471
BfmI	CTRYAG	1 cut(s)	54
BfuI	GTATCC	1 cut(s)	886
BglI	GCCNNNNNGGC	1 cut(s)	210
BisI	GCNGC	8 cut(s)	54, 57, 205, 242, 605, 990, 1085, 1199
BlsI	GCNGC	8 cut(s)	55, 58, 206, 243, 606, 991, 1086, 1200
Bme1390I	CCNGG	1 cut(s)	1162
Bme18I	GGWCC	1 cut(s)	137
BmeT110I	CYCGRG	1 cut(s)	276
BmgT120I	GGNCC	3 cut(s)	137, 829, 971
BmiI	GGNNCC	4 cut(s)	139, 214, 577, 766
BmrFI	CCNGG	1 cut(s)	1162
BmsI	GCATC	2 cut(s)	700, 1137
BpiI	GAAGAC	2 cut(s)	247, 654
BpmI	CTGGAG	1 cut(s)	1187
BsaI	GGTCTC	2 cut(s)	1084, 1151
BsaJI	CCNNGG	2 cut(s)	133, 811
Bsc4I	CCNNNNNNNGG	1 cut(s)	1094
Bse1I	ACTGG	2 cut(s)	265, 577
Bse3DI	GCAATG	4 cut(s)	186, 225, 669, 1157
BseBI	CCWGG	1 cut(s)	1162
BseDI	CCNNGG	2 cut(s)	133, 811
BseGI	GGATG	3 cut(s)	160, 725, 1152
BseLI	CCNNNNNNNGG	1 cut(s)	1094
BseMI	GCAATG	4 cut(s)	186, 225, 669, 1157
BseMII	CTCAG	2 cut(s)	279, 720
BseNI	ACTGG	2 cut(s)	265, 577
BseRI	GAGGAG	3 cut(s)	211, 694, 928
BseXI	GCAGC	5 cut(s)	40, 68, 253, 976, 1185
Bsh1236I	CGCG	1 cut(s)	207
BshFI	GGCC	3 cut(s)	831, 860, 972
BsiHKCI	CYCGRG	1 cut(s)	276
BsiSI	CCGG	1 cut(s)	1088
BslFI	GGGAC	2 cut(s)	114, 129
BslI	CCNNNNNNNGG	1 cut(s)	1094
BsmAI	GTCTC	6 cut(s)	238, 286, 326, 1084, 1151, 1179
BsmFI	GGGAC	2 cut(s)	114, 129
BsmI	GAATGC	2 cut(s)	530, 664
BsnI	GGCC	3 cut(s)	831, 860, 972
Bso31I	GGTCTC	2 cut(s)	1084, 1151
BsoBI	CYCGRG	1 cut(s)	276
Bsp143I	GATC	4 cut(s)	427, 884, 1048, 1120
Bsp19I	CCATGG	1 cut(s)	811
BspACI	CCGC	3 cut(s)	205, 604, 1085
BspANI	GGCC	3 cut(s)	831, 860, 972
BspCNI	CTCAG	2 cut(s)	278, 719
BspFNI	CGCG	1 cut(s)	207
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	4 cut(s)	139, 214, 577, 766
BspMAI	CTGCAG	1 cut(s)	58
BspPI	GGATC	3 cut(s)	892, 1056, 1128
BspTNI	GGTCTC	2 cut(s)	1084, 1151
BsrDI	GCAATG	4 cut(s)	186, 225, 669, 1157
BsrI	ACTGG	2 cut(s)	265, 577
BssECI	CCNNGG	2 cut(s)	133, 811
BssMI	GATC	4 cut(s)	427, 884, 1048, 1120
BssNAI	GTATAC	1 cut(s)	570
BssT1I	CCWWGG	1 cut(s)	811
Bst1107I	GTATAC	1 cut(s)	570
Bst2UI	CCWGG	1 cut(s)	1162
Bst4CI	ACNGT	6 cut(s)	49, 317, 592, 623, 1112, 1168
Bst6I	CTCTTC	1 cut(s)	1086
BstAPI	GCANNNNNTGC	2 cut(s)	97, 534
BstDEI	CTNAG	2 cut(s)	265, 706
BstDSI	CCRYGG	2 cut(s)	133, 811
BstF5I	GGATG	3 cut(s)	160, 725, 1152
BstFNI	CGCG	1 cut(s)	207
BstKTI	GATC	4 cut(s)	430, 887, 1051, 1123
BstMAI	GTCTC	6 cut(s)	238, 286, 326, 1084, 1151, 1179
BstMBI	GATC	4 cut(s)	427, 884, 1048, 1120
BstMWI	GCNNNNNNNGC	3 cut(s)	97, 210, 534
BstNI	CCWGG	1 cut(s)	1162
BstSCI	CCNGG	1 cut(s)	1160
BstSFI	CTRYAG	1 cut(s)	54
BstUI	CGCG	1 cut(s)	207
BstV1I	GCAGC	5 cut(s)	40, 68, 253, 976, 1185
BstV2I	GAAGAC	2 cut(s)	247, 654
BstXI	CCANNNNNNTGG	1 cut(s)	683
BstZ17I	GTATAC	1 cut(s)	570
BsuI	GTATCC	1 cut(s)	886
BsuRI	GGCC	3 cut(s)	831, 860, 972
BtgI	CCRYGG	2 cut(s)	133, 811
BtsCI	GGATG	3 cut(s)	160, 725, 1152
BtsI	GCAGTG	1 cut(s)	181
BtsIMutI	CAGTG	4 cut(s)	181, 258, 619, 886
CaiI	CAGNNNCTG	1 cut(s)	53
CciI	TCATGA	1 cut(s)	937
Cfr13I	GGNCC	3 cut(s)	137, 829, 971
Csp6I	GTAC	2 cut(s)	273, 344
CviAII	CATG	7 cut(s)	362, 581, 600, 644, 812, 938, 1182
CviQI	GTAC	2 cut(s)	273, 344
DdeI	CTNAG	2 cut(s)	265, 706
DpnI	GATC	4 cut(s)	429, 886, 1050, 1122
DpnII	GATC	4 cut(s)	427, 884, 1048, 1120
DraIII	CACNNNGTG	1 cut(s)	625
DrdI	GACNNNNNNGTC	2 cut(s)	590, 1171
DseDI	GACNNNNNNGTC	2 cut(s)	590, 1171
EaeI	YGGCCR	1 cut(s)	858
Eam1104I	CTCTTC	1 cut(s)	1086
EarI	CTCTTC	1 cut(s)	1086
Eco130I	CCWWGG	1 cut(s)	811
Eco31I	GGTCTC	2 cut(s)	1084, 1151
Eco47I	GGWCC	1 cut(s)	137
Eco57I	CTGAAG	2 cut(s)	318, 741
Eco88I	CYCGRG	1 cut(s)	276
EcoO109I	RGGNCCY	1 cut(s)	971
EcoRII	CCWGG	1 cut(s)	1160
EcoT14I	CCWWGG	1 cut(s)	811
EcoT22I	ATGCAT	1 cut(s)	530
ErhI	CCWWGG	1 cut(s)	811
FaeI	CATG	7 cut(s)	365, 584, 603, 647, 815, 941, 1185
FaqI	GGGAC	2 cut(s)	114, 129
FatI	CATG	7 cut(s)	361, 580, 599, 643, 811, 937, 1181
FauNDI	CATATG	1 cut(s)	67
FblI	GTMKAC	1 cut(s)	569
Fnu4HI	GCNGC	8 cut(s)	54, 57, 205, 242, 605, 990, 1085, 1199
FokI	GGATG	3 cut(s)	147, 712, 1159
Fsp4HI	GCNGC	8 cut(s)	54, 57, 205, 242, 605, 990, 1085, 1199
FspBI	CTAG	2 cut(s)	126, 471
GluI	GCNGC	8 cut(s)	54, 57, 205, 242, 605, 990, 1085, 1199
GsuI	CTGGAG	1 cut(s)	1187
HaeIII	GGCC	3 cut(s)	831, 860, 972
HapII	CCGG	1 cut(s)	1088
Hin1II	CATG	7 cut(s)	365, 584, 603, 647, 815, 941, 1185
HinfI	GANTC	1 cut(s)	629
HpaII	CCGG	1 cut(s)	1088
HphI	GGTGA	4 cut(s)	142, 350, 370, 682
Hpy166II	GTNNAC	4 cut(s)	137, 570, 628, 673
Hpy188I	TCNGA	5 cut(s)	268, 404, 709, 760, 889
Hpy188III	TCNNGA	4 cut(s)	492, 865, 938, 1204
Hpy8I	GTNNAC	4 cut(s)	137, 570, 628, 673
HpyAV	CCTTC	6 cut(s)	177, 203, 778, 917, 1012, 1188
HpyCH4III	ACNGT	6 cut(s)	49, 317, 592, 623, 1112, 1168
HpyCH4V	TGCA	7 cut(s)	56, 186, 230, 356, 528, 662, 1150
HpyF10VI	GCNNNNNNNGC	3 cut(s)	97, 210, 534
HpyF3I	CTNAG	2 cut(s)	265, 706
Hsp92II	CATG	7 cut(s)	365, 584, 603, 647, 815, 941, 1185
Kzo9I	GATC	4 cut(s)	427, 884, 1048, 1120
LmnI	GCTCC	4 cut(s)	218, 581, 707, 1206
Lsp1109I	GCAGC	5 cut(s)	40, 68, 253, 976, 1185
LweI	GCATC	2 cut(s)	700, 1137
MaeI	CTAG	2 cut(s)	126, 471
MaeIII	GTNAC	1 cut(s)	421
MalI	GATC	4 cut(s)	429, 886, 1050, 1122
MboI	GATC	4 cut(s)	427, 884, 1048, 1120
MboII	GAAGA	7 cut(s)	247, 447, 659, 747, 932, 1103, 1137
MlsI	TGGCCA	1 cut(s)	860
MluCI	AATT	4 cut(s)	33, 95, 433, 900
MluNI	TGGCCA	1 cut(s)	860
MlyI	GAGTC	1 cut(s)	623
MmeI	TCCRAC	2 cut(s)	753, 936
Mox20I	TGGCCA	1 cut(s)	860
Mph1103I	ATGCAT	1 cut(s)	530
MroXI	GAANNNNTTC	1 cut(s)	1129
MscI	TGGCCA	1 cut(s)	860
MseI	TTAA	5 cut(s)	11, 716, 807, 1059, 1074
Msp20I	TGGCCA	1 cut(s)	860
MspI	CCGG	1 cut(s)	1088
MspR9I	CCNGG	1 cut(s)	1162
Mva1269I	GAATGC	2 cut(s)	530, 664
MvaI	CCWGG	1 cut(s)	1162
MvnI	CGCG	1 cut(s)	207
MwoI	GCNNNNNNNGC	3 cut(s)	97, 210, 534
NcoI	CCATGG	1 cut(s)	811
NdeI	CATATG	1 cut(s)	67
NdeII	GATC	4 cut(s)	427, 884, 1048, 1120
NlaIII	CATG	7 cut(s)	365, 584, 603, 647, 815, 941, 1185
NlaIV	GGNNCC	4 cut(s)	139, 214, 577, 766
NmuCI	GTSAC	1 cut(s)	421
NsiI	ATGCAT	1 cut(s)	530
PagI	TCATGA	1 cut(s)	937
PctI	GAATGC	2 cut(s)	530, 664
PdmI	GAANNNNTTC	1 cut(s)	1129
PflFI	GACNNNGTC	1 cut(s)	141
PkrI	GCNGC	8 cut(s)	55, 58, 206, 243, 606, 991, 1086, 1200
PleI	GAGTC	1 cut(s)	623
PpsI	GAGTC	1 cut(s)	623
PsiI	TTATAA	1 cut(s)	611
Psp6I	CCWGG	1 cut(s)	1160
PspGI	CCWGG	1 cut(s)	1160
PspN4I	GGNNCC	4 cut(s)	139, 214, 577, 766
PspPI	GGNCC	3 cut(s)	137, 829, 971
PstI	CTGCAG	1 cut(s)	58
PstNI	CAGNNNCTG	1 cut(s)	53
PsyI	GACNNNGTC	1 cut(s)	141
RsaI	GTAC	2 cut(s)	274, 345
RsaNI	GTAC	2 cut(s)	273, 344
SaqAI	TTAA	5 cut(s)	11, 716, 807, 1059, 1074
SatI	GCNGC	8 cut(s)	54, 57, 205, 242, 605, 990, 1085, 1199
Sau3AI	GATC	4 cut(s)	427, 884, 1048, 1120
Sau96I	GGNCC	3 cut(s)	137, 829, 971
SchI	GAGTC	1 cut(s)	623
ScrFI	CCNGG	1 cut(s)	1162
SfaNI	GCATC	2 cut(s)	700, 1137
SfcI	CTRYAG	1 cut(s)	54
SinI	GGWCC	1 cut(s)	137
Sse9I	AATT	4 cut(s)	33, 95, 433, 900
SsiI	CCGC	3 cut(s)	205, 604, 1085
SspMI	CTAG	2 cut(s)	126, 471
StyD4I	CCNGG	1 cut(s)	1160
StyI	CCWWGG	1 cut(s)	811
TaaI	ACNGT	6 cut(s)	49, 317, 592, 623, 1112, 1168
TaqI	TCGA	4 cut(s)	31, 864, 1123, 1133
TaqII	GACCGA	1 cut(s)	409
TasI	AATT	4 cut(s)	33, 95, 433, 900
TauI	GCSGC	3 cut(s)	207, 607, 1087
Tru1I	TTAA	5 cut(s)	11, 716, 807, 1059, 1074
Tru9I	TTAA	5 cut(s)	11, 716, 807, 1059, 1074
TscAI	CASTG	4 cut(s)	188, 265, 626, 886
TseFI	GTSAC	1 cut(s)	421
TseI	GCWGC	5 cut(s)	53, 56, 241, 989, 1198
Tsp45I	GTSAC	1 cut(s)	421
TspDTI	ATGAA	4 cut(s)	660, 926, 954, 1170
TspGWI	ACGGA	1 cut(s)	777
TspRI	CASTG	4 cut(s)	188, 265, 626, 886
Tth111I	GACNNNGTC	1 cut(s)	141
VpaK11BI	GGWCC	1 cut(s)	137
XapI	RAATTY	1 cut(s)	33
XcmI	CCANNNNNNNNNTGG	1 cut(s)	622
XmiI	GTMKAC	1 cut(s)	569
XmnI	GAANNNNTTC	1 cut(s)	1129
XspI	CTAG	2 cut(s)	126, 471
Zsp2I	ATGCAT	1 cut(s)	530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.