Rw2G033270

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004527
Physical Location & Seq
Reverse (-)
54505116 .. 54506560
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033270.1

Sequence Viewer

Length: 1161 bp
ATGGAGTTTCATGGAGATCAAAGAAACCTCTGGAGCTTCATGGATGAAGAAAGAAAGAGCATCAAGTTACCTCGGAGACTTTCCTTTGGTAGTAACCAACAACAAAGTTCATCATCCGAAGTACCTAACATTCACTTAGCTTCTGCAGCAAGACCTTCTAATGCTGCTTCAGAACCATCAATTGATGCAATTCCTCCCAGACCTATGAGCCCCAGCACGCCATGGACACTCTCCCCGGTGCGCACTTCTCCTTCCCAACCTCTTCTTTATCATTGCATAGCCTCCCTCCACCGCCATGAAGGTAACATTTTCTCCATCACACTCTCAAAAGATTTCATATTCACCGGCTCAGAAAGTAGCCGGATTCATGCTTGGAAGCAACCAGATTGCACCGAGATAGGTTACATAAAGGCTACTTCGGGTCAAGTTCGAGCCATATTGGCCTATGGGAAAACTGCTGTTCACCACACATGCCAGCAACACAATGATCACATCTCTTGCCTCGCTTACAACAAAGAAGACAAGCTGTTGTACACAGGCTCGTGGGACAAATCTGTTAAAGCTTGGAAGATAAACGAAAAGCGTTGTGTCGACTCGTTCATTGCTCATGAAGGTCATGTGAATGGGATTGTGATCAACCAGGAAGATGGGTGTGTTTTCACTTGCTCATCAGATGGCTCAGTCAAAATTTGGAGAAGGGTGTTTGGTGAAAGTTCACACATACTCACCATGACCCTGAAGTTTCAACTTTCACCAGTGAATGCCTTGGCATTGAGCTTGTCAAATAGCACCACCCTCCTCTACTCTGGCTCATCGGATGGGCTCATAAACTTCTGGGAAAAAGAGAAGATGTCTGGAAGATACAACCATGGTGGGTTTCTGCAAGGTCATCATTTCGCCGTTCTTTGCTTGGTAGCCTTGGGAGAACTAATCTTCAGTGGCTCTGAGGATGCAACCATTAGGGTTTGGAGGAGAGAGGAAGGAAATTGCTTCCATTCTTGTCTTTCTGTCATTGAGGGACATCACGGGCCGGTTAGATGCTTAGCAGTGTCTTTGGAAACAGAAAATGTGGTAAGGGTAAAGGGTTTGTTGGTTTATAGTGCAAGCTTAGACCAAACTTTTAAGGTGTGGAGGGTTAAGGTTTTTCCACTGAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

386

Amino Acids

43.14

Weight (kDa)

8.44

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 89 - 232 1.8e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 91 - 244 2.6e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 92 - 327 4.9e-17 WDR3 second beta-propeller domain
Beta-prop_ACSF4 PF13570 99 - 330 9.6e-07 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_IP5PC_F PF23754 106 - 233 4e-06 IP5P C-F beta-propeller
Beta-prop_THOC3 PF25174 137 - 244 5.6e-12 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 146 - 243 3.7e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 151 - 280 6.8e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 151 - 281 3.8e-12 CDC20/Fizzy WD40 domain
WDR55 PF24796 152 - 382 8e-15 WDR55
Beta-prop_TEP1_2nd PF25047 154 - 243 2e-08 TEP-1 second beta-propeller
WD40 PF00400 156 - 189 1.1e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 157 - 323 2.9e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 160 - 245 3.4e-07 EIF3I
WD40_Prp19 PF24814 161 - 282 1.1e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 167 - 303 9.1e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 195 - 231 8e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 244 - 380 9.5e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 257 - 332 2.7e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 258 - 379 1e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 264 - 357 1.6e-10 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 308 - 380 1.6e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 242
AccI GTMKAC 1 cut(s) 591
AciI CCGC 1 cut(s) 292
AcsI RAATTY 1 cut(s) 687
AcuI CTGAAG 3 cut(s) 153, 758, 919
AfaI GTAC 2 cut(s) 123, 533
AgsI TTSAA 1 cut(s) 746
AjnI CCWGG 1 cut(s) 639
AluBI AGCT 6 cut(s) 36, 140, 526, 563, 777, 1107
AluI AGCT 6 cut(s) 36, 140, 526, 563, 777, 1107
Alw26I GTCTC 1 cut(s) 70
AoxI GGCC 2 cut(s) 441, 1028
ApeKI GCWGC 2 cut(s) 146, 164
ApoI RAATTY 1 cut(s) 687
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 1028
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 5 cut(s) 334, 455, 718, 719, 744
BanII GRGCYC 2 cut(s) 212, 825
BauI CACGAG 1 cut(s) 541
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 151, 158
BccI CCATC 5 cut(s) 184, 323, 641, 668, 812
BceAI ACGGC 1 cut(s) 884
BciT130I CCWGG 1 cut(s) 641
BclI TGATCA 2 cut(s) 487, 633
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 70
BfmI CTRYAG 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 2 cut(s) 147, 165
BlpI GCTNAGC 1 cut(s) 1042
BlsI GCNGC 2 cut(s) 148, 166
Bme1390I CCNGG 2 cut(s) 236, 641
BmgT120I GGNCC 1 cut(s) 1028
BmrFI CCNGG 2 cut(s) 236, 641
BmsI GCATC 4 cut(s) 69, 175, 940, 1028
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 52
Bpu1102I GCTNAGC 1 cut(s) 1042
BpuMI CCSGG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 632
BsaJI CCNNGG 6 cut(s) 71, 221, 234, 765, 868, 918
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bse118I RCCGGY 2 cut(s) 344, 1030
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 2 cut(s) 271, 600
Bse8I GATNNNNATC 1 cut(s) 632
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 6 cut(s) 71, 221, 234, 765, 868, 918
BseGI GGATG 4 cut(s) 49, 113, 823, 955
BseJI GATNNNNATC 1 cut(s) 632
BseMI GCAATG 2 cut(s) 271, 600
BseMII CTCAG 4 cut(s) 363, 693, 936, 1142
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 2 cut(s) 788, 985
BseXI GCAGC 2 cut(s) 151, 158
BseYI CCCAGC 1 cut(s) 212
BshFI GGCC 2 cut(s) 443, 1030
BsiSI CCGG 4 cut(s) 236, 345, 361, 1031
BslFI GGGAC 2 cut(s) 560, 1032
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 560, 1032
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 2 cut(s) 443, 1030
Bsp1286I GDGCHC 2 cut(s) 212, 825
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 3 cut(s) 16, 487, 633
Bsp1720I GCTNAGC 1 cut(s) 1042
Bsp19I CCATGG 2 cut(s) 221, 868
BspACI CCGC 1 cut(s) 292
BspANI GGCC 2 cut(s) 443, 1030
BspCNI CTCAG 4 cut(s) 362, 692, 937, 1143
BspHI TCATGA 1 cut(s) 607
BspMAI CTGCAG 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 271, 600
BsrFI RCCGGY 2 cut(s) 344, 1030
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 2 cut(s) 344, 1030
BssECI CCNNGG 6 cut(s) 71, 221, 234, 765, 868, 918
BssMI GATC 3 cut(s) 16, 487, 633
BssSI CACGAG 1 cut(s) 541
BssT1I CCWWGG 4 cut(s) 221, 765, 868, 918
Bst2BI CACGAG 1 cut(s) 541
Bst2UI CCWGG 1 cut(s) 641
Bst6I CTCTTC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 218, 476, 1105
BstDEI CTNAG 7 cut(s) 136, 349, 679, 945, 1042, 1108, 1151
BstDSI CCRYGG 2 cut(s) 221, 868
BstF5I GGATG 4 cut(s) 49, 113, 823, 955
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 3 cut(s) 19, 490, 636
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 3 cut(s) 16, 487, 633
BstMWI GCNNNNNNNGC 2 cut(s) 146, 440
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 234, 639
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 151, 158
BstV2I GAAGAC 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 647
BsuRI GGCC 2 cut(s) 443, 1030
BtgI CCRYGG 2 cut(s) 221, 868
BtsCI GGATG 4 cut(s) 49, 113, 823, 955
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 4 cut(s) 762, 943, 1053, 1148
Cac8I GCNNGC 3 cut(s) 218, 476, 1105
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 2 cut(s) 344, 1030
Cfr13I GGNCC 1 cut(s) 1028
Csp6I GTAC 2 cut(s) 122, 532
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviQI GTAC 2 cut(s) 122, 532
DdeI CTNAG 7 cut(s) 136, 349, 679, 945, 1042, 1108, 1151
DpnI GATC 3 cut(s) 18, 489, 635
DpnII GATC 3 cut(s) 16, 487, 633
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco130I CCWWGG 4 cut(s) 221, 765, 868, 918
Eco24I GRGCYC 2 cut(s) 212, 825
Eco57I CTGAAG 3 cut(s) 153, 758, 919
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 4 cut(s) 221, 765, 868, 918
EcoT38I GRGCYC 2 cut(s) 212, 825
ErhI CCWWGG 4 cut(s) 221, 765, 868, 918
FaqI GGGAC 2 cut(s) 560, 1032
FbaI TGATCA 2 cut(s) 487, 633
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 2 cut(s) 147, 165
FokI GGATG 4 cut(s) 56, 100, 830, 962
FriOI GRGCYC 2 cut(s) 212, 825
Fsp4HI GCNGC 2 cut(s) 147, 165
FspAI RTGCGCAY 1 cut(s) 242
FspI TGCGCA 1 cut(s) 242
GlaI GCGC 1 cut(s) 242
GluI GCNGC 2 cut(s) 147, 165
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 52
HaeIII GGCC 2 cut(s) 443, 1030
HapII CCGG 4 cut(s) 236, 345, 361, 1031
HhaI GCGC 1 cut(s) 243
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 2 cut(s) 561, 1105
HinfI GANTC 2 cut(s) 364, 593
HpaII CCGG 4 cut(s) 236, 345, 361, 1031
HphI GGTGA 5 cut(s) 334, 455, 718, 719, 744
Hpy166II GTNNAC 4 cut(s) 463, 534, 592, 716
Hpy188I TCNGA 7 cut(s) 75, 118, 172, 352, 673, 817, 946
Hpy188III TCNNGA 3 cut(s) 31, 608, 855
Hpy8I GTNNAC 4 cut(s) 463, 534, 592, 716
HpyAV CCTTC 6 cut(s) 165, 261, 293, 605, 690, 974
HpyCH4V TGCA 7 cut(s) 146, 188, 276, 390, 883, 953, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 440
HpyF3I CTNAG 7 cut(s) 136, 349, 679, 945, 1042, 1108, 1151
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 487, 633
Kzo9I GATC 3 cut(s) 16, 487, 633
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 2 cut(s) 151, 158
LweI GCATC 4 cut(s) 69, 175, 940, 1028
MaeIII GTNAC 4 cut(s) 66, 92, 302, 401
MalI GATC 3 cut(s) 18, 489, 635
MboI GATC 3 cut(s) 16, 487, 633
MboII GAAGA 8 cut(s) 59, 254, 530, 580, 656, 859, 870, 925
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 212, 825
MluCI AATT 4 cut(s) 180, 189, 687, 985
MlyI GAGTC 1 cut(s) 587
MseI TTAA 3 cut(s) 558, 1122, 1137
MslI CAYNNNNRTG 2 cut(s) 294, 621
MspI CCGG 4 cut(s) 236, 345, 361, 1031
MspR9I CCNGG 2 cut(s) 236, 641
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 1 cut(s) 641
MwoI GCNNNNNNNGC 2 cut(s) 146, 440
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 2 cut(s) 221, 868
NdeII GATC 3 cut(s) 16, 487, 633
NsbI TGCGCA 1 cut(s) 242
NspI RCATGY 1 cut(s) 474
PagI TCATGA 1 cut(s) 607
PctI GAATGC 1 cut(s) 766
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 2 cut(s) 148, 166
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 639
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 1 cut(s) 639
PspPI GGNCC 1 cut(s) 1028
PstI CTGCAG 1 cut(s) 148
RsaI GTAC 2 cut(s) 123, 533
RsaNI GTAC 2 cut(s) 122, 532
RseI CAYNNNNRTG 2 cut(s) 294, 621
SalI GTCGAC 1 cut(s) 590
SaqAI TTAA 3 cut(s) 558, 1122, 1137
SatI GCNGC 2 cut(s) 147, 165
Sau3AI GATC 3 cut(s) 16, 487, 633
Sau96I GGNCC 1 cut(s) 1028
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 2 cut(s) 236, 641
SduI GDGCHC 2 cut(s) 212, 825
SfaNI GCATC 4 cut(s) 69, 175, 940, 1028
SfcI CTRYAG 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 294, 621
Sse9I AATT 4 cut(s) 180, 189, 687, 985
SsiI CCGC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 234, 639
StyI CCWWGG 4 cut(s) 221, 765, 868, 918
TaqI TCGA 2 cut(s) 430, 591
TasI AATT 4 cut(s) 180, 189, 687, 985
TatI WGTACW 1 cut(s) 531
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 558, 1122, 1137
Tru9I TTAA 3 cut(s) 558, 1122, 1137
TscAI CASTG 4 cut(s) 762, 943, 1053, 1155
TseI GCWGC 2 cut(s) 146, 164
TspDTI ATGAA 8 cut(s) 28, 60, 99, 312, 325, 356, 589, 624
TspRI CASTG 4 cut(s) 762, 943, 1053, 1155
XapI RAATTY 1 cut(s) 687
XceI RCATGY 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.