pycom09g12370

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004527
Physical Location & Seq
Forward (+)
10893251 .. 10894610
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12370.5

Sequence Viewer

Length: 1128 bp
ATGGAGCTTCATGGACGAAGAGCGAAAGAGCATCAATTCATCAGAACCCGAAAACATTCGTTCGGCTTCTGCAGCAAGACCGGACATAGCTGCGTCCGAACCAACAATACCTCCGAGGCCTATGAGCCCTGGGACACCGTGGACACTCTCCCCGGTGCGCACCTCTCCCTCCCAATCCCTTCTTTACCACTGCATTGCCTCCCTTCACCCTCAGAGAGTAGCCGGATTCATGTTTGGAAGCAACCAGATTGCACTGAAATCGGTTGCATAAAGGCTACTTCAGGTCAAATTCGAGCTATCTTGGCCTCCGGGAAAATCTTATTCACCGCACACGGCGACTGCAAAATTCGCATATGGAATGCGTCGAATGCGGAGAATTTTCGACCGAAGAAGATCACATGTACGCTTCCTCAAAGAGGCCGGTTTTCGCTGTTTTCAAAAAAGAGCAGCCAGCAGCACAACGATCGCATCTCTTGCATGGAAGATCAACGAAAGGCGCTGCGTGGATTCGTTTGTAGCTCACGAAGGCCACGTAAATGGAATCGCAATCAACCAAGAAGACGGCTGCGTGTTCACTTGCTCCTCGGACGGCTCAGTCAAGATTTGGAGAAGGGTGTTCGCTTGTCATCCACGACCACCCTCCTCTACTCCGGCTCGTCAGATGGGCTAATAAACTTTTGGGAAAAGGAGAAGATGTCCGGGAGGTACAACCACGGAGGGTTTCTGCAAGGTCATCATTTTGCCGTCCTTTGCTTGGTAGCGTTAGGAAACCTAGTTTTCAGTGGCTCCGAGGACTCAACGATTAGGGTTTGGAGAAGAGAGGAAGGGAACTGCTTCCACTCATGTCTCTCCGTGATAGAAGGGCATCATGGACCGGTGAGATGCTTGGCAGTGGCTTTGGAGACGGATAATTTGGTAAGGGTGAAAGGTTTGTTGGTTTACAGTGCAAGTTTGGATCGAACCTTTAAGGTTTGGAGGGTTAAAGTTTTTCCTGCTGAGAAGGTAAACTTGGTGGATGAAACTGCTGCTGCCAAAGACCCACAGAGAGAGATTGTGGAGTGTGAGACAAGCCCTGTGTTGTCACCTTCATGGGTGGAGAAAAAGCTTCAAGGCAACTATTTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00565 ko01100 map00565 map01100
Pfam Domains
Protein Families
WD40

Protein Analysis

376

Amino Acids

42.47

Weight (kDa)

9.64

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 594
Acc16I TGCGCA 1 cut(s) 159
AciI CCGC 2 cut(s) 327, 371
AclWI GGATC 1 cut(s) 963
AcsI RAATTY 3 cut(s) 288, 345, 376
AcuI CTGAAG 1 cut(s) 264
AfaI GTAC 2 cut(s) 403, 707
AfiI CCNNNNNNNGG 2 cut(s) 416, 754
AflIII ACRYGT 1 cut(s) 398
AgeI ACCGGT 1 cut(s) 874
AgsI TTSAA 2 cut(s) 438, 1109
AjnI CCWGG 1 cut(s) 128
AjuI GAANNNNNNNTTGG 4 cut(s) 917, 949, 992, 1024
AluBI AGCT 5 cut(s) 7, 90, 296, 519, 1105
AluI AGCT 5 cut(s) 7, 90, 296, 519, 1105
Alw26I GTCTC 3 cut(s) 851, 896, 1058
AlwI GGATC 1 cut(s) 963
AoxI GGCC 4 cut(s) 117, 303, 418, 527
ApeKI GCWGC 8 cut(s) 72, 90, 447, 454, 499, 565, 1025, 1028
ApoI RAATTY 3 cut(s) 288, 345, 376
ArsI GACNNNNNNTTYG 2 cut(s) 895, 927
AsiGI ACCGGT 1 cut(s) 874
Asp700I GAANNNNTTC 2 cut(s) 55, 833
AspLEI GCGC 2 cut(s) 160, 499
AspS9I GGNCC 1 cut(s) 872
AsuC2I CCSGG 3 cut(s) 153, 310, 700
AsuHPI GGTGA 5 cut(s) 198, 316, 889, 934, 1074
AvaII GGWCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 565
BbvI GCAGC 8 cut(s) 77, 84, 459, 466, 486, 552, 1012, 1015
BccI CCATC 1 cut(s) 656
BceAI ACGGC 4 cut(s) 349, 578, 605, 728
BcgI CGANNNNNNTGC 4 cut(s) 241, 275, 446, 480
BciT130I CCWGG 1 cut(s) 130
BcnI CCSGG 3 cut(s) 153, 310, 700
BcoDI GTCTC 3 cut(s) 851, 896, 1058
BfaI CTAG 1 cut(s) 773
BfmI CTRYAG 1 cut(s) 70
BfoI RGCGCY 1 cut(s) 500
BisI GCNGC 8 cut(s) 73, 91, 448, 455, 500, 566, 1026, 1029
BlsI GCNGC 8 cut(s) 74, 92, 449, 456, 501, 567, 1027, 1030
Bme1390I CCNGG 4 cut(s) 130, 153, 310, 700
Bme18I GGWCC 1 cut(s) 872
BmgT120I GGNCC 1 cut(s) 872
BmiI GGNNCC 1 cut(s) 787
BmrFI CCNGG 4 cut(s) 130, 153, 310, 700
BmsI GCATC 4 cut(s) 40, 477, 872, 874
BpiI GAAGAC 1 cut(s) 565
BpuMI CCSGG 3 cut(s) 153, 310, 700
BsaAI YACGTR 1 cut(s) 533
BsaJI CCNNGG 8 cut(s) 114, 128, 129, 138, 151, 583, 712, 789
BsaWI WCCGGW 2 cut(s) 80, 874
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 680, 710
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 754
Bse118I RCCGGY 2 cut(s) 420, 874
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 8 cut(s) 114, 128, 129, 138, 151, 583, 712, 789
BseGI GGATG 2 cut(s) 626, 1021
BseLI CCNNNNNNNGG 2 cut(s) 416, 754
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 225, 607, 987
BseRI GAGGAG 2 cut(s) 572, 632
BseXI GCAGC 8 cut(s) 77, 84, 459, 466, 486, 552, 1012, 1015
Bsh1285I CGRYCG 2 cut(s) 386, 466
BshFI GGCC 4 cut(s) 119, 305, 420, 529
BshTI ACCGGT 1 cut(s) 874
BsiEI CGRYCG 2 cut(s) 386, 466
BsiSI CCGG 8 cut(s) 81, 153, 223, 309, 421, 651, 699, 875
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 2 cut(s) 416, 754
BsmAI GTCTC 3 cut(s) 851, 896, 1058
BsmBI CGTCTC 1 cut(s) 896
BsmFI GGGAC 1 cut(s) 146
BsmI GAATGC 2 cut(s) 364, 373
BsnI GGCC 4 cut(s) 119, 305, 420, 529
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 4 cut(s) 393, 463, 484, 955
BspACI CCGC 2 cut(s) 327, 371
BspANI GGCC 4 cut(s) 119, 305, 420, 529
BspCNI CTCAG 3 cut(s) 224, 606, 988
BspLI GGNNCC 1 cut(s) 787
BspMAI CTGCAG 1 cut(s) 74
BspPI GGATC 1 cut(s) 963
BspQI GCTCTTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 2 cut(s) 420, 874
BssAI RCCGGY 2 cut(s) 420, 874
BssECI CCNNGG 8 cut(s) 114, 128, 129, 138, 151, 583, 712, 789
BssMI GATC 4 cut(s) 393, 463, 484, 955
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 2 cut(s) 139, 944
Bst6I CTCTTC 2 cut(s) 13, 811
BstAPI GCANNNNNTGC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 3 cut(s) 211, 593, 996
BstDSI CCRYGG 2 cut(s) 138, 712
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 2 cut(s) 626, 1021
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 2 cut(s) 160, 499
BstKTI GATC 4 cut(s) 396, 466, 487, 958
BstMAI GTCTC 3 cut(s) 851, 896, 1058
BstMBI GATC 4 cut(s) 393, 463, 484, 955
BstMCI CGRYCG 2 cut(s) 386, 466
BstMWI GCNNNNNNNGC 5 cut(s) 72, 302, 348, 368, 474
BstNI CCWGG 1 cut(s) 130
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 4 cut(s) 128, 151, 308, 698
BstSFI CTRYAG 1 cut(s) 70
BstV1I GCAGC 8 cut(s) 77, 84, 459, 466, 486, 552, 1012, 1015
BstV2I GAAGAC 1 cut(s) 565
BstXI CCANNNNNNTGG 1 cut(s) 537
BsuRI GGCC 4 cut(s) 119, 305, 420, 529
BtgI CCRYGG 2 cut(s) 138, 712
BtsCI GGATG 2 cut(s) 626, 1021
BtsI GCAGTG 2 cut(s) 188, 897
BtsIMutI CAGTG 5 cut(s) 188, 252, 787, 897, 949
Cac8I GCNNGC 1 cut(s) 452
CfoI GCGC 2 cut(s) 160, 499
Cfr10I RCCGGY 2 cut(s) 420, 874
Cfr13I GGNCC 1 cut(s) 872
CseI GACGC 2 cut(s) 82, 351
Csp6I GTAC 2 cut(s) 402, 706
CspAI ACCGGT 1 cut(s) 874
CviAII CATG 7 cut(s) 11, 230, 399, 478, 843, 869, 1089
CviQI GTAC 2 cut(s) 402, 706
DdeI CTNAG 3 cut(s) 211, 593, 996
DpnI GATC 4 cut(s) 395, 465, 486, 957
DpnII GATC 4 cut(s) 393, 463, 484, 955
DrdI GACNNNNNNGTC 1 cut(s) 594
DseDI GACNNNNNNGTC 1 cut(s) 594
Eam1104I CTCTTC 2 cut(s) 13, 811
EarI CTCTTC 2 cut(s) 13, 811
Eco147I AGGCCT 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 264
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 896
FaeI CATG 7 cut(s) 14, 233, 402, 481, 846, 872, 1092
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FaqI GGGAC 1 cut(s) 146
FatI CATG 7 cut(s) 10, 229, 398, 477, 842, 868, 1088
FauNDI CATATG 1 cut(s) 353
Fnu4HI GCNGC 8 cut(s) 73, 91, 448, 455, 500, 566, 1026, 1029
FokI GGATG 2 cut(s) 613, 1028
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 8 cut(s) 73, 91, 448, 455, 500, 566, 1026, 1029
FspAI RTGCGCAY 1 cut(s) 159
FspBI CTAG 1 cut(s) 773
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 2 cut(s) 159, 498
GluI GCNGC 8 cut(s) 73, 91, 448, 455, 500, 566, 1026, 1029
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 4 cut(s) 119, 305, 420, 529
HapII CCGG 8 cut(s) 81, 153, 223, 309, 421, 651, 699, 875
HgaI GACGC 2 cut(s) 82, 351
HhaI GCGC 2 cut(s) 160, 499
Hin1II CATG 7 cut(s) 14, 233, 402, 481, 846, 872, 1092
Hin6I GCGC 2 cut(s) 158, 497
HinP1I GCGC 2 cut(s) 158, 497
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 4 cut(s) 226, 507, 541, 794
HpaII CCGG 8 cut(s) 81, 153, 223, 309, 421, 651, 699, 875
HphI GGTGA 5 cut(s) 198, 316, 889, 934, 1074
Hpy166II GTNNAC 4 cut(s) 142, 574, 940, 1006
Hpy188I TCNGA 7 cut(s) 44, 98, 115, 214, 587, 661, 790
Hpy188III TCNNGA 2 cut(s) 522, 599
Hpy8I GTNNAC 4 cut(s) 142, 574, 940, 1006
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 8 cut(s) 189, 213, 519, 604, 818, 854, 994, 1095
HpyCH4III ACNGT 2 cut(s) 139, 944
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 8 cut(s) 72, 193, 252, 267, 342, 477, 727, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 302, 348, 368, 474
HpyF3I CTNAG 3 cut(s) 211, 593, 996
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 7 cut(s) 14, 233, 402, 481, 846, 872, 1092
HspAI GCGC 2 cut(s) 158, 497
Kzo9I GATC 4 cut(s) 393, 463, 484, 955
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 3 cut(s) 4, 585, 791
Lsp1109I GCAGC 8 cut(s) 77, 84, 459, 466, 486, 552, 1012, 1015
LweI GCATC 4 cut(s) 40, 477, 872, 874
MaeI CTAG 1 cut(s) 773
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 1 cut(s) 1080
MalI GATC 4 cut(s) 395, 465, 486, 957
MboI GATC 4 cut(s) 393, 463, 484, 955
MboII GAAGA 7 cut(s) 30, 400, 403, 494, 570, 703, 828
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 5 cut(s) 35, 288, 345, 376, 910
MlyI GAGTC 1 cut(s) 788
MroXI GAANNNNTTC 2 cut(s) 55, 833
MseI TTAA 2 cut(s) 966, 981
MslI CAYNNNNRTG 2 cut(s) 535, 1087
MspI CCGG 8 cut(s) 81, 153, 223, 309, 421, 651, 699, 875
MspR9I CCNGG 4 cut(s) 130, 153, 310, 700
Mva1269I GAATGC 2 cut(s) 364, 373
MvaI CCWGG 1 cut(s) 130
MwoI GCNNNNNNNGC 5 cut(s) 72, 302, 348, 368, 474
NciI CCSGG 3 cut(s) 153, 310, 700
NdeI CATATG 1 cut(s) 353
NdeII GATC 4 cut(s) 393, 463, 484, 955
NlaIII CATG 7 cut(s) 14, 233, 402, 481, 846, 872, 1092
NlaIV GGNNCC 1 cut(s) 787
NmuCI GTSAC 1 cut(s) 1080
NsbI TGCGCA 1 cut(s) 159
NspI RCATGY 1 cut(s) 402
PasI CCCWGGG 1 cut(s) 129
PceI AGGCCT 1 cut(s) 119
PciI ACATGT 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PctI GAATGC 2 cut(s) 364, 373
PdmI GAANNNNTTC 2 cut(s) 55, 833
PfeI GAWTC 3 cut(s) 226, 507, 541
PfoI TCCNGGA 2 cut(s) 308, 698
PinAI ACCGGT 1 cut(s) 874
PkrI GCNGC 8 cut(s) 74, 92, 449, 456, 501, 567, 1027, 1030
Ple19I CGATCG 1 cut(s) 466
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
Ppu21I YACGTR 1 cut(s) 533
PscI ACATGT 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 128
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 787
PspPI GGNCC 1 cut(s) 872
PsrI GAACNNNNNNTAC 2 cut(s) 91, 123
PstI CTGCAG 1 cut(s) 74
PvuI CGATCG 1 cut(s) 466
RsaI GTAC 2 cut(s) 403, 707
RsaNI GTAC 2 cut(s) 402, 706
RseI CAYNNNNRTG 2 cut(s) 535, 1087
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 2 cut(s) 966, 981
SatI GCNGC 8 cut(s) 73, 91, 448, 455, 500, 566, 1026, 1029
Sau3AI GATC 4 cut(s) 393, 463, 484, 955
Sau96I GGNCC 1 cut(s) 872
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 4 cut(s) 130, 153, 310, 700
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 4 cut(s) 40, 477, 872, 874
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 872
SmiMI CAYNNNNRTG 2 cut(s) 535, 1087
Sse9I AATT 5 cut(s) 35, 288, 345, 376, 910
SseBI AGGCCT 1 cut(s) 119
SsiI CCGC 2 cut(s) 327, 371
SspMI CTAG 1 cut(s) 773
StuI AGGCCT 1 cut(s) 119
StyD4I CCNGG 4 cut(s) 128, 151, 308, 698
TaaI ACNGT 2 cut(s) 139, 944
TaiI ACGT 1 cut(s) 535
TaqI TCGA 4 cut(s) 292, 365, 382, 958
TaqII GACCGA 1 cut(s) 400
TasI AATT 5 cut(s) 35, 288, 345, 376, 910
TfiI GAWTC 3 cut(s) 226, 507, 541
Tru1I TTAA 2 cut(s) 966, 981
Tru9I TTAA 2 cut(s) 966, 981
TscAI CASTG 5 cut(s) 195, 259, 787, 897, 949
TseFI GTSAC 1 cut(s) 1080
TseI GCWGC 8 cut(s) 72, 90, 447, 454, 499, 565, 1025, 1028
Tsp45I GTSAC 1 cut(s) 1080
TspDTI ATGAA 4 cut(s) 28, 218, 1032, 1077
TspGWI ACGGA 3 cut(s) 729, 841, 920
TspRI CASTG 5 cut(s) 195, 259, 787, 897, 949
VpaK11BI GGWCC 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 3 cut(s) 288, 345, 376
XceI RCATGY 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 55, 833
XspI CTAG 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.