FvH4_2g37530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005878
Physical Location & Seq
Reverse (-)
27322841 .. 27327479
4639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37530.t1

Sequence Viewer

Length: 1377 bp
ATGGAGGTGGCCACCACCAGAGGCTTCGCGACTCACCTCAGCCGCCACCGTCCTCCGCCGCATCCCCTCCACCGCCACAAGGACGAGCTCAGCAAGCGGCGGAGGCTCACCGTTGGCGGCGGCCGACCCGGAGCGAGTCGAATCAGCTGCTGCTGCTCCGACTCGGTGGTTCCGATTCGGAGCAACGCCTCCTCGGAGGGGAAGAGGAAGACGTGCGACGAGTGGCGGTTCGATTCCAAGAACCAGAGGCCTCACAGAGCTGGACTCAAAGCTGCTGCTGCATTGCCCTTCACTTCTGCTCAATCTCAATTTGCGTCCAAGCAAAACAACAAGCCTCGCCGTCGGCCTCGCTGTGCTCCCGGAAACACCGGACCGCAATCTCGTGATACTCCGCCGAAAAGAGATACTGGAATTGCGAATGAGAAGGACTGGGGCATCAGCTTGTTGAATGAGCACGTGAAGGAGTCAGGCACTAATGAAGATGGAAGTACTTGGTACCGAGAAAGCGGGGAGGAGCTGGGGGACACTGGATACAGATGTAGGTGGACAAGGATGGGTGGTGCTTCGCATGACTCGTCTTCCGAGTGGAGAGAAGAGTGGTGGGAGAAAAGTGACTGGACCGGATACAAAGAGCTAGGTGTGGAGAAATCTGGCAGAAATGCCGAAGGGGATTCATGGTGGGAAACGTGGCAAGAGGTTCTCCATCAAGATGAATGGAGTAACCTAGCAAGGATTGAGAGAAGTGCACAGAAACGAGCAAAATCAGGCACTGAAAATGCTGGATGGTCTGAGAAATGGTGGGAGAAATATGATGCCAAAGGCTGGACTGAGAAAGGAGCACACAAATGGGGTAGACTTAATGAACAGTCTTGGTGGGAGAAATGGGGAGAGCACTATGATGGAAGGGGATCTGTCCTCAAATGGACAGATAAATGGGCAGAGACTGCACTTGGAACCAAATGGGGAGACAAGTGGGAAGAGAAGTTCTTTGAGGGTATAGGTTCACGACAAGGGGAAACATGGCATGTAACTTCCAATGATGAACGTTGGTCAAGGACATGGGGAGAAGAACACATAGGAAATGGTAAAGTGCACAAATATGGGAAGAGCACAACAGGAGAAAGCTGGGATATTGTTGTGGATGAGGAGACCTATTACGAGGCTGTGCCGCATTACGGATGGGCGGATGTTGTTGGCGATTCTAGCCAGCTACTATCAATTAAACCTCGTCCAAAGCCGCCTGAGGGCTACCCAAAGTTTGACTTTACGAGACCAGCAACAGGATTGGGTACACCTCTTCCATCAACTAAACCACCACCTCCAGCAGACGAACCACCTCGAGAAGATGAACCGCCAGAGGAAGTTTCTCCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

459

Amino Acids

52.33

Weight (kDa)

6.97

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 822
AccI GTMKAC 1 cut(s) 853
AccII CGCG 1 cut(s) 29
AclI AACGTT 1 cut(s) 1045
AclWI GGATC 1 cut(s) 916
AcoI YGGCCR 2 cut(s) 9, 121
AcvI CACGTG 1 cut(s) 457
AfaI GTAC 3 cut(s) 490, 497, 1291
AfiI CCNNNNNNNGG 6 cut(s) 79, 198, 822, 1175, 1244, 1280
AgsI TTSAA 1 cut(s) 448
AjiI CACGTC 1 cut(s) 213
AloI GAACNNNNNNTCC 2 cut(s) 968, 1000
AluBI AGCT 9 cut(s) 88, 147, 260, 272, 441, 517, 634, 1125, 1210
AluI AGCT 9 cut(s) 88, 147, 260, 272, 441, 517, 634, 1125, 1210
Alw21I GWGCWC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
Alw26I GTCTC 4 cut(s) 935, 960, 1142, 1264
Alw44I GTGCAC 2 cut(s) 744, 1091
AlwI GGATC 1 cut(s) 916
AlwNI CAGNNNCTG 3 cut(s) 150, 770, 944
Ama87I CYCGRG 1 cut(s) 1338
AoxI GGCC 4 cut(s) 9, 121, 248, 344
ApaLI GTGCAC 2 cut(s) 744, 1091
ApeKI GCWGC 6 cut(s) 147, 150, 153, 272, 275, 278
Asp718I GGTACC 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 371, 618
AsuC2I CCSGG 2 cut(s) 129, 360
AsuHPI GGTGA 2 cut(s) 26, 100
AvaI CYCGRG 1 cut(s) 1338
AvaII GGWCC 2 cut(s) 371, 618
AxyI CCTNAGG 1 cut(s) 1242
BaeGI GKGCMC 2 cut(s) 748, 1095
BaeI ACNNNNGTAYC 2 cut(s) 523, 556
BalI TGGCCA 1 cut(s) 11
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 90
BauI CACGAG 1 cut(s) 381
BbrPI CACGTG 1 cut(s) 457
BbsI GAAGAC 2 cut(s) 215, 570
Bbv12I GWGCWC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
BbvCI CCTCAGC 1 cut(s) 38
BbvI GCAGC 6 cut(s) 134, 137, 140, 259, 262, 265
BccI CCATC 7 cut(s) 476, 547, 711, 777, 893, 1173, 1309
BceAI ACGGC 1 cut(s) 324
BcgI CGANNNNNNTGC 2 cut(s) 129, 163
BciVI GTATCC 2 cut(s) 524, 617
BcnI CCSGG 2 cut(s) 129, 360
BcoDI GTCTC 4 cut(s) 935, 960, 1142, 1264
BfaI CTAG 3 cut(s) 635, 725, 1203
BfuI GTATCC 2 cut(s) 524, 617
BlpI GCTNAGC 1 cut(s) 89
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 2 cut(s) 129, 360
Bme18I GGWCC 2 cut(s) 371, 618
BmeT110I CYCGRG 1 cut(s) 1338
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 371, 618
BmiI GGNNCC 3 cut(s) 171, 497, 955
BmrFI CCNGG 2 cut(s) 129, 360
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 3 cut(s) 70, 444, 802
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 2 cut(s) 215, 570
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpmI CTGGAG 1 cut(s) 1305
Bpu10I CCTNAGC 1 cut(s) 38
Bpu1102I GCTNAGC 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 129, 360
BsaAI YACGTR 1 cut(s) 457
BsaI GGTCTC 2 cut(s) 1142, 1264
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 2 cut(s) 368, 620
Bsc4I CCNNNNNNNGG 6 cut(s) 79, 198, 822, 1175, 1244, 1280
Bse1I ACTGG 4 cut(s) 412, 434, 532, 620
Bse21I CCTNAGG 1 cut(s) 1242
Bse3DI GCAATG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 6 cut(s) 61, 558, 788, 1147, 1184, 1192
BseLI CCNNNNNNNGG 6 cut(s) 79, 198, 822, 1175, 1244, 1280
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 5 cut(s) 52, 103, 780, 819, 1233
BseNI ACTGG 4 cut(s) 412, 434, 532, 620
BseRI GAGGAG 3 cut(s) 181, 527, 1160
BseSI GKGCMC 2 cut(s) 748, 1095
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 6 cut(s) 134, 137, 140, 259, 262, 265
BseYI CCCAGC 2 cut(s) 517, 1125
BsgI GTGCAG 1 cut(s) 930
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 4 cut(s) 11, 123, 250, 346
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
BsiHKCI CYCGRG 1 cut(s) 1338
BsiSI CCGG 4 cut(s) 129, 360, 369, 621
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 6 cut(s) 79, 198, 822, 1175, 1244, 1280
BsmAI GTCTC 4 cut(s) 935, 960, 1142, 1264
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 4 cut(s) 11, 123, 250, 346
Bso31I GGTCTC 2 cut(s) 1142, 1264
BsoBI CYCGRG 1 cut(s) 1338
Bsp1286I GDGCHC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
Bsp143I GATC 1 cut(s) 908
Bsp1720I GCTNAGC 1 cut(s) 89
Bsp68I TCGCGA 1 cut(s) 29
BspANI GGCC 4 cut(s) 11, 123, 250, 346
BspCNI CTCAG 5 cut(s) 51, 102, 781, 820, 1234
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 3 cut(s) 171, 497, 955
BspPI GGATC 1 cut(s) 916
BspQI GCTCTTC 1 cut(s) 1100
BspT107I GGYRCC 1 cut(s) 495
BspTNI GGTCTC 2 cut(s) 1142, 1264
BsrDI GCAATG 1 cut(s) 281
BsrI ACTGG 4 cut(s) 412, 434, 532, 620
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 1 cut(s) 908
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 3 cut(s) 50, 112, 867
Bst6I CTCTTC 5 cut(s) 197, 588, 972, 1100, 1302
BstAPI GCANNNNNTGC 1 cut(s) 944
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 95, 1208
BstDEI CTNAG 5 cut(s) 38, 89, 789, 828, 1242
BstF5I GGATG 6 cut(s) 61, 558, 788, 1147, 1184, 1192
BstFNI CGCG 1 cut(s) 29
BstKTI GATC 1 cut(s) 911
BstMAI GTCTC 4 cut(s) 935, 960, 1142, 1264
BstMBI GATC 1 cut(s) 908
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 6 cut(s) 94, 103, 153, 278, 944, 1203
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 2 cut(s) 127, 358
BstSLI GKGCMC 2 cut(s) 748, 1095
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 6 cut(s) 134, 137, 140, 259, 262, 265
BstV2I GAAGAC 2 cut(s) 215, 570
BstX2I RGATCY 1 cut(s) 908
BstYI RGATCY 1 cut(s) 908
BstZI CGGCCG 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 524, 617
BsuRI GGCC 4 cut(s) 11, 123, 250, 346
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 6 cut(s) 61, 558, 788, 1147, 1184, 1192
BtsIMutI CAGTG 2 cut(s) 525, 768
BtuMI TCGCGA 1 cut(s) 29
Cac8I GCNNGC 2 cut(s) 95, 1208
CaiI CAGNNNCTG 3 cut(s) 150, 770, 944
Cfr13I GGNCC 2 cut(s) 371, 618
CpoI CGGWCCG 1 cut(s) 371
CseI GACGC 1 cut(s) 303
Csp6I GTAC 3 cut(s) 489, 496, 1290
CspI CGGWCCG 1 cut(s) 371
CviAII CATG 5 cut(s) 569, 675, 1020, 1025, 1059
CviQI GTAC 3 cut(s) 489, 496, 1290
DdeI CTNAG 5 cut(s) 38, 89, 789, 828, 1242
DpnI GATC 1 cut(s) 910
DpnII GATC 1 cut(s) 908
EaeI YGGCCR 2 cut(s) 9, 121
EagI CGGCCG 1 cut(s) 121
Eam1104I CTCTTC 5 cut(s) 197, 588, 972, 1100, 1302
EarI CTCTTC 5 cut(s) 197, 588, 972, 1100, 1302
EciI GGCGGA 4 cut(s) 45, 115, 381, 1199
Ecl136II GAGCTC 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 90
Eco31I GGTCTC 2 cut(s) 1142, 1264
Eco47I GGWCC 2 cut(s) 371, 618
Eco52I CGGCCG 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 88
Eco72I CACGTG 1 cut(s) 457
Eco81I CCTNAGG 1 cut(s) 1242
Eco88I CYCGRG 1 cut(s) 1338
EcoICRI GAGCTC 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 90
FaeI CATG 5 cut(s) 572, 678, 1023, 1028, 1062
FalI AAGNNNNNCTT 2 cut(s) 1247, 1279
FaqI GGGAC 1 cut(s) 536
FatI CATG 5 cut(s) 568, 674, 1019, 1024, 1058
FauI CCCGC 1 cut(s) 500
FblI GTMKAC 1 cut(s) 853
FokI GGATG 6 cut(s) 48, 565, 795, 1154, 1191, 1199
FriOI GRGCYC 1 cut(s) 90
FspBI CTAG 3 cut(s) 635, 725, 1203
GsaI CCCAGC 2 cut(s) 521, 1129
GsuI CTGGAG 1 cut(s) 1305
HaeIII GGCC 4 cut(s) 11, 123, 250, 346
HapII CCGG 4 cut(s) 129, 360, 369, 621
HgaI GACGC 1 cut(s) 303
Hin1II CATG 5 cut(s) 572, 678, 1023, 1028, 1062
HpaII CCGG 4 cut(s) 129, 360, 369, 621
HphI GGTGA 2 cut(s) 26, 100
Hpy166II GTNNAC 6 cut(s) 546, 746, 854, 1004, 1093, 1292
Hpy188I TCNGA 6 cut(s) 160, 174, 180, 196, 583, 790
Hpy188III TCNNGA 5 cut(s) 28, 383, 707, 1005, 1340
Hpy8I GTNNAC 6 cut(s) 546, 746, 854, 1004, 1093, 1292
Hpy99I CGWCG 2 cut(s) 221, 345
HpyAV CCTTC 5 cut(s) 298, 418, 454, 659, 897
HpyCH4III ACNGT 3 cut(s) 50, 112, 867
HpyCH4IV ACGT 4 cut(s) 212, 456, 686, 1045
HpyCH4V TGCA 4 cut(s) 281, 746, 947, 1093
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 103, 153, 278, 944, 1203
HpyF3I CTNAG 5 cut(s) 38, 89, 789, 828, 1242
HpySE526I ACGT 4 cut(s) 212, 456, 686, 1045
Hsp92II CATG 5 cut(s) 572, 678, 1023, 1028, 1062
KpnI GGTACC 1 cut(s) 499
Kzo9I GATC 1 cut(s) 908
LguI GCTCTTC 1 cut(s) 1100
LmnI GCTCC 6 cut(s) 131, 161, 180, 361, 514, 836
Lsp1109I GCAGC 6 cut(s) 134, 137, 140, 259, 262, 265
LweI GCATC 3 cut(s) 70, 444, 802
MaeI CTAG 3 cut(s) 635, 725, 1203
MaeII ACGT 4 cut(s) 212, 456, 686, 1045
MaeIII GTNAC 3 cut(s) 611, 719, 1027
MalI GATC 1 cut(s) 910
MboI GATC 1 cut(s) 908
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 3 cut(s) 308, 411, 1218
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 6 cut(s) 25, 145, 155, 258, 473, 566
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 11
MscI TGGCCA 1 cut(s) 11
MseI TTAA 2 cut(s) 858, 1221
MslI CAYNNNNRTG 4 cut(s) 708, 844, 897, 1098
Msp20I TGGCCA 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 4 cut(s) 129, 360, 369, 621
MspR9I CCNGG 2 cut(s) 129, 360
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 6 cut(s) 94, 103, 153, 278, 944, 1203
NciI CCSGG 2 cut(s) 129, 360
NdeII GATC 1 cut(s) 908
NlaIII CATG 5 cut(s) 572, 678, 1023, 1028, 1062
NlaIV GGNNCC 3 cut(s) 171, 497, 955
NmuCI GTSAC 1 cut(s) 611
NruI TCGCGA 1 cut(s) 29
NspI RCATGY 1 cut(s) 1028
PaeR7I CTCGAG 1 cut(s) 1338
PceI AGGCCT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 572
PfeI GAWTC 5 cut(s) 141, 175, 233, 671, 1199
PflMI CCANNNNNTGG 1 cut(s) 822
PfoI TCCNGGA 1 cut(s) 358
PleI GAGTC 6 cut(s) 25, 144, 155, 258, 472, 566
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 6 cut(s) 25, 144, 155, 258, 472, 566
Ppu21I YACGTR 1 cut(s) 457
Psp124BI GAGCTC 1 cut(s) 90
Psp1406I AACGTT 1 cut(s) 1045
PspCI CACGTG 1 cut(s) 457
PspFI CCCAGC 2 cut(s) 517, 1125
PspN4I GGNNCC 3 cut(s) 171, 497, 955
PspPI GGNCC 2 cut(s) 371, 618
PstNI CAGNNNCTG 3 cut(s) 150, 770, 944
PsuI RGATCY 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 147
RruI TCGCGA 1 cut(s) 29
RsaI GTAC 3 cut(s) 490, 497, 1291
RsaNI GTAC 3 cut(s) 489, 496, 1290
RseI CAYNNNNRTG 4 cut(s) 708, 844, 897, 1098
Rsr2I CGGWCCG 1 cut(s) 371
RsrII CGGWCCG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 90
SapI GCTCTTC 1 cut(s) 1100
SaqAI TTAA 2 cut(s) 858, 1221
Sau3AI GATC 1 cut(s) 908
Sau96I GGNCC 2 cut(s) 371, 618
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 6 cut(s) 25, 145, 155, 258, 473, 566
ScrFI CCNGG 2 cut(s) 129, 360
SduI GDGCHC 8 cut(s) 90, 358, 456, 748, 841, 894, 1095, 1112
SfaNI GCATC 3 cut(s) 70, 444, 802
Sfr274I CTCGAG 1 cut(s) 1338
SinI GGWCC 2 cut(s) 371, 618
SlaI CTCGAG 1 cut(s) 1338
SmiMI CAYNNNNRTG 4 cut(s) 708, 844, 897, 1098
SmlI CTYRAG 1 cut(s) 1338
SmoI CTYRAG 1 cut(s) 1338
Sse9I AATT 3 cut(s) 308, 411, 1218
SseBI AGGCCT 1 cut(s) 250
SspMI CTAG 3 cut(s) 635, 725, 1203
SstI GAGCTC 1 cut(s) 90
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 2 cut(s) 127, 358
TaaI ACNGT 3 cut(s) 50, 112, 867
TaiI ACGT 4 cut(s) 215, 459, 689, 1048
TaqI TCGA 3 cut(s) 139, 231, 1339
TasI AATT 3 cut(s) 308, 411, 1218
TatI WGTACW 1 cut(s) 488
TauI GCSGC 7 cut(s) 45, 61, 100, 120, 123, 1171, 1240
TfiI GAWTC 5 cut(s) 141, 175, 233, 671, 1199
Tru1I TTAA 2 cut(s) 858, 1221
Tru9I TTAA 2 cut(s) 858, 1221
TscAI CASTG 2 cut(s) 532, 775
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 6 cut(s) 147, 150, 153, 272, 275, 278
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 6 cut(s) 492, 663, 726, 876, 1056, 1362
TspGWI ACGGA 1 cut(s) 1191
TspRI CASTG 2 cut(s) 532, 775
Van91I CCANNNNNTGG 1 cut(s) 822
VneI GTGCAC 2 cut(s) 744, 1091
VpaK11BI GGWCC 2 cut(s) 371, 618
XceI RCATGY 1 cut(s) 1028
XhoI CTCGAG 1 cut(s) 1338
XmiI GTMKAC 1 cut(s) 853
XspI CTAG 3 cut(s) 635, 725, 1203
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.