Rroxscaffold_7G00163980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005878
Physical Location & Seq
Reverse (-)
6089335 .. 6094015
4681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163980.1

Sequence Viewer

Length: 1422 bp
ATGGCGGCGGCCACCACCACCACTAGAGGCTTCGCGACTCACCCCAGCCACCTCTCCTTCCTCCATCATCTACCGCATCCGCACCGGGGCAATGCTCACCCCGCCGACGTGGACGAGCTGAGCAAGCGGCGGAGGCTCACCATCGGCGGAAACCGAGTCGGAGTGAGTCGAATCAGTTGCTGCTGCTCCGACTCGGCTGTTCCGATTCGGAGTAGCTCCCCCGCCGAGAAGAGGAACAAGTGCGATGAGCGGCGGTTCGATTCCAACAACAGGCCTCCTCACAGAGCTGGACTCAAAGCTGCTGCTGCATTGCCCTTTGCTTCTGAGCAATCTCAATTTGCCTCCAAACAAAACAGCAAGGTGTACCCTCGCCGGCGCCCTCGCTGTACTCCGGGGAATACTGGTCCGCAATCTCGTGATACTCCGCCGAAAAGAGATACTGGTATTGCCAGTGAGAAGGAATGGGGTATTAGCTTGTTAAACGAACATGTGAAGGAATCCGGCACTAATGAAGATGGAAGTACTTGGTACCGAGAAAGCGGGGAGGAGCTGGGGAACACTGGGTACAGATGTAGGTGGACAAGGATGGGTGGTGCTTCTCATGACTCTTCTTCAGAGTGGAGAGAAGAGTGGTGGGAGAAAAGTGACTGGACTGGATACAAAGAGCTAGGCATTATTTGCATCATTTATATTTTCATAGGTGTGGAGAAATCTGGTAGAAATGCCGAAGGGGATTCATGGTGGGAAACCTGGCAAGAGGTTCTTCATCAAGATGAATGGAGTAACCTAGCAAGGATTGAGAGAAGTGCACAGAAACGAGCAAAATCAGGCACTGAAAATGCTGGATGGTCTGAGAAATGGTGGGAGAAATATGATGCCAAAGGTTGGACTGAGAAAGGAGCACACAAGTGGGGTAGACTTAATGAACAGTCTTGGTGGGAGAAGTGGGGAGAGCATTATGACGGAAGGGGATCAGTTCTCAAATGGACAGATAAATGGGCAGAGACTGAACTTGGAACCAAATGGGGAGACAAGTGGGAAGAGAAGTTCTTTGAGGGTATAGGTTCACGACAAGGGGAAACATGGCATGTAACTTCCAATGATGAACGTTGGTCAAGGACATGGGGAGAAGAACACATAGGAAATGGTAAAGTGCACAAATATGGGAAGAGCACAACAGGTGAAAGCTGGGATATTGTTGTGGATGAGGAGACCTATTACGAGGCTGTGCCGCATTACGGATGGGCAGATGTTGTAGGCGATTCTACCCAGTTACTATCAATTGAACCTCGTGAAAAGCCGCCTGAGGTCTACCCGAACTTTGACTTTACGAGATCACCACCTGCAATGGGTACACCGCTTCCAGAGAATGAACCACCACCACCTCCAGAGGATGAACCACCGGATGAATTTTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

473

Amino Acids

54.1

Weight (kDa)

5.92

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1351
Acc36I ACCTGC 1 cut(s) 1351
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 2 cut(s) 375, 528
AccB7I CCANNNNNTGG 1 cut(s) 885
AccBSI CCGCTC 1 cut(s) 250
AccI GTMKAC 2 cut(s) 916, 1311
AccII CGCG 1 cut(s) 35
AclI AACGTT 1 cut(s) 1108
AclWI GGATC 1 cut(s) 979
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 1409
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 1 cut(s) 376
AfaI GTAC 6 cut(s) 365, 388, 523, 530, 566, 1354
AfiI CCNNNNNNNGG 6 cut(s) 86, 231, 270, 885, 1238, 1349
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 1 cut(s) 1286
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 749
AleI CACNNNNGTG 1 cut(s) 907
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 8 cut(s) 118, 216, 287, 299, 474, 550, 667, 1188
AluI AGCT 8 cut(s) 118, 216, 287, 299, 474, 550, 667, 1188
Alw21I GWGCWC 4 cut(s) 811, 904, 1158, 1175
Alw26I GTCTC 3 cut(s) 998, 1023, 1205
Alw44I GTGCAC 2 cut(s) 807, 1154
AlwI GGATC 1 cut(s) 979
AlwNI CAGNNNCTG 3 cut(s) 180, 833, 1007
AoxI GGCC 2 cut(s) 9, 272
ApaLI GTGCAC 2 cut(s) 807, 1154
ApeKI GCWGC 5 cut(s) 180, 183, 299, 302, 305
ApoI RAATTY 1 cut(s) 1409
Asp718I GGTACC 1 cut(s) 528
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 2 cut(s) 86, 393
AsuHPI GGTGA 5 cut(s) 32, 89, 130, 1193, 1329
AvaII GGWCC 1 cut(s) 404
AxyI CCTNAGG 1 cut(s) 1305
BaeGI GKGCMC 2 cut(s) 811, 1158
BaeI ACNNNNGTAYC 2 cut(s) 556, 589
BanI GGYRCC 2 cut(s) 375, 528
BauI CACGAG 2 cut(s) 414, 1290
Bbv12I GWGCWC 4 cut(s) 811, 904, 1158, 1175
BbvI GCAGC 5 cut(s) 167, 170, 286, 289, 292
BccI CCATC 6 cut(s) 72, 149, 509, 580, 840, 1236
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BciT130I CCWGG 1 cut(s) 751
BciVI GTATCC 1 cut(s) 650
BcnI CCSGG 2 cut(s) 86, 393
BcoDI GTCTC 3 cut(s) 998, 1023, 1205
BfaI CTAG 3 cut(s) 24, 668, 788
BfoI RGCGCY 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 1351
BfuI GTATCC 1 cut(s) 650
BlpI GCTNAGC 1 cut(s) 119
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 3 cut(s) 86, 393, 751
Bme18I GGWCC 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 3 cut(s) 377, 530, 1018
BmrFI CCNGG 3 cut(s) 86, 393, 751
BmrI ACTGGG 2 cut(s) 570, 1264
BmsI GCATC 3 cut(s) 85, 690, 865
BmuI ACTGGG 2 cut(s) 570, 1264
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 1 cut(s) 1371
Bpu1102I GCTNAGC 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 86, 393
BsaHI GRCGYC 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 2 cut(s) 85, 392
BsaWI WCCGGW 1 cut(s) 1402
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 231, 270, 885, 1238, 1349
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 7 cut(s) 406, 445, 450, 565, 653, 658, 1270
Bse21I CCTNAGG 1 cut(s) 1305
Bse3DI GCAATG 3 cut(s) 97, 308, 1353
BseBI CCWGG 1 cut(s) 751
BseDI CCNNGG 2 cut(s) 85, 392
BseGI GGATG 7 cut(s) 76, 591, 851, 1210, 1247, 1399, 1411
BseLI CCNNNNNNNGG 6 cut(s) 86, 231, 270, 885, 1238, 1349
BseMI GCAATG 3 cut(s) 97, 308, 1353
BseMII CTCAG 5 cut(s) 110, 315, 843, 882, 1296
BseNI ACTGG 7 cut(s) 406, 445, 450, 565, 653, 658, 1270
BseRI GAGGAG 3 cut(s) 267, 560, 1223
BseSI GKGCMC 2 cut(s) 811, 1158
BseXI GCAGC 5 cut(s) 167, 170, 286, 289, 292
BseYI CCCAGC 3 cut(s) 44, 550, 1188
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 11, 274
BshNI GGYRCC 2 cut(s) 375, 528
BsiHKAI GWGCWC 4 cut(s) 811, 904, 1158, 1175
BsiSI CCGG 5 cut(s) 85, 373, 392, 501, 1403
BslI CCNNNNNNNGG 6 cut(s) 86, 231, 270, 885, 1238, 1349
BsmAI GTCTC 3 cut(s) 998, 1023, 1205
BsnI GGCC 2 cut(s) 11, 274
Bso31I GGTCTC 1 cut(s) 1205
Bsp1286I GDGCHC 4 cut(s) 811, 904, 1158, 1175
Bsp143I GATC 2 cut(s) 971, 1334
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp68I TCGCGA 1 cut(s) 35
BspANI GGCC 2 cut(s) 11, 274
BspCNI CTCAG 5 cut(s) 111, 316, 844, 883, 1297
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 3 cut(s) 377, 530, 1018
BspMI ACCTGC 1 cut(s) 1351
BspPI GGATC 1 cut(s) 979
BspQI GCTCTTC 1 cut(s) 1163
BspT107I GGYRCC 2 cut(s) 375, 528
BspTNI GGTCTC 1 cut(s) 1205
BsrBI CCGCTC 1 cut(s) 250
BsrDI GCAATG 3 cut(s) 97, 308, 1353
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 7 cut(s) 406, 445, 450, 565, 653, 658, 1270
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 85, 392
BssMI GATC 2 cut(s) 971, 1334
BssNI GRCGYC 1 cut(s) 376
BssSI CACGAG 2 cut(s) 414, 1290
Bst2BI CACGAG 2 cut(s) 414, 1290
Bst2UI CCWGG 1 cut(s) 751
Bst4CI ACNGT 1 cut(s) 930
Bst6I CTCTTC 5 cut(s) 224, 613, 621, 1035, 1163
BstACI GRCGYC 1 cut(s) 376
BstAPI GCANNNNNTGC 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 125, 374
BstDEI CTNAG 5 cut(s) 119, 324, 852, 891, 1305
BstF5I GGATG 7 cut(s) 76, 591, 851, 1210, 1247, 1399, 1411
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 2 cut(s) 974, 1337
BstMAI GTCTC 3 cut(s) 998, 1023, 1205
BstMBI GATC 2 cut(s) 971, 1334
BstMWI GCNNNNNNNGC 5 cut(s) 101, 124, 133, 305, 678
BstNI CCWGG 1 cut(s) 751
BstNSI RCATGY 2 cut(s) 491, 1091
BstSCI CCNGG 3 cut(s) 84, 391, 749
BstSLI GKGCMC 2 cut(s) 811, 1158
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 5 cut(s) 167, 170, 286, 289, 292
Bsu36I CCTNAGG 1 cut(s) 1305
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 2 cut(s) 11, 274
BtgZI GCGATG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 7 cut(s) 76, 591, 851, 1210, 1247, 1399, 1411
BtsIMutI CAGTG 3 cut(s) 457, 558, 831
BtuMI TCGCGA 1 cut(s) 35
BveI ACCTGC 1 cut(s) 1351
Cac8I GCNNGC 2 cut(s) 125, 374
CaiI CAGNNNCTG 3 cut(s) 180, 833, 1007
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 6 cut(s) 364, 387, 522, 529, 565, 1353
CviAII CATG 6 cut(s) 488, 602, 738, 1083, 1088, 1122
CviQI GTAC 6 cut(s) 364, 387, 522, 529, 565, 1353
DdeI CTNAG 5 cut(s) 119, 324, 852, 891, 1305
DinI GGCGCC 1 cut(s) 377
DpnI GATC 2 cut(s) 973, 1336
DpnII GATC 2 cut(s) 971, 1334
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 5 cut(s) 224, 613, 621, 1035, 1163
EarI CTCTTC 5 cut(s) 224, 613, 621, 1035, 1163
EciI GGCGGA 3 cut(s) 145, 162, 414
Eco147I AGGCCT 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 1205
Eco47I GGWCC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 597
Eco81I CCTNAGG 1 cut(s) 1305
EcoRII CCWGG 1 cut(s) 749
EgeI GGCGCC 1 cut(s) 377
EheI GGCGCC 1 cut(s) 377
FaeI CATG 6 cut(s) 491, 605, 741, 1086, 1091, 1125
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FatI CATG 6 cut(s) 487, 601, 737, 1082, 1087, 1121
FauI CCCGC 3 cut(s) 109, 229, 533
FblI GTMKAC 2 cut(s) 916, 1311
FokI GGATG 7 cut(s) 63, 598, 858, 1217, 1254, 1406, 1418
FspBI CTAG 3 cut(s) 24, 668, 788
GlaI GCGC 1 cut(s) 377
GsaI CCCAGC 3 cut(s) 48, 554, 1192
GsuI CTGGAG 1 cut(s) 1371
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 2 cut(s) 11, 274
HapII CCGG 5 cut(s) 85, 373, 392, 501, 1403
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 376
Hin1II CATG 6 cut(s) 491, 605, 741, 1086, 1091, 1125
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HpaII CCGG 5 cut(s) 85, 373, 392, 501, 1403
HphI GGTGA 5 cut(s) 32, 89, 130, 1193, 1329
Hpy166II GTNNAC 9 cut(s) 112, 364, 579, 809, 917, 1067, 1156, 1312, 1355
Hpy188I TCNGA 7 cut(s) 161, 190, 204, 210, 325, 616, 853
Hpy188III TCNNGA 8 cut(s) 34, 416, 602, 770, 1068, 1292, 1364, 1388
Hpy8I GTNNAC 9 cut(s) 112, 364, 579, 809, 917, 1067, 1156, 1312, 1355
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 5 cut(s) 67, 451, 487, 722, 960
HpyCH4III ACNGT 1 cut(s) 930
HpyCH4IV ACGT 2 cut(s) 108, 1108
HpyCH4V TGCA 5 cut(s) 308, 681, 809, 1156, 1346
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 124, 133, 305, 678
HpyF3I CTNAG 5 cut(s) 119, 324, 852, 891, 1305
HpySE526I ACGT 2 cut(s) 108, 1108
Hsp92I GRCGYC 1 cut(s) 376
Hsp92II CATG 6 cut(s) 491, 605, 741, 1086, 1091, 1125
HspAI GCGC 1 cut(s) 376
KasI GGCGCC 1 cut(s) 375
KpnI GGTACC 1 cut(s) 532
KroI GCCGGC 1 cut(s) 372
KroNI GCCGGC 1 cut(s) 374
Kzo9I GATC 2 cut(s) 971, 1334
LguI GCTCTTC 1 cut(s) 1163
LmnI GCTCC 4 cut(s) 191, 221, 547, 899
Lsp1109I GCAGC 5 cut(s) 167, 170, 286, 289, 292
LweI GCATC 3 cut(s) 85, 690, 865
MaeI CTAG 3 cut(s) 24, 668, 788
MaeII ACGT 2 cut(s) 108, 1108
MaeIII GTNAC 4 cut(s) 644, 782, 1090, 1272
MalI GATC 2 cut(s) 973, 1336
MbiI CCGCTC 1 cut(s) 250
MboI GATC 2 cut(s) 971, 1334
MboII GAAGA 9 cut(s) 241, 524, 600, 603, 638, 755, 1052, 1142, 1180
MfeI CAATTG 1 cut(s) 1281
MhlI GDGCHC 4 cut(s) 811, 904, 1158, 1175
MluCI AATT 3 cut(s) 335, 1281, 1409
Mly113I GGCGCC 1 cut(s) 376
MlyI GAGTC 6 cut(s) 31, 165, 175, 185, 285, 599
MmeI TCCRAC 4 cut(s) 139, 213, 288, 866
MreI CGCCGGCG 1 cut(s) 372
MroNI GCCGGC 1 cut(s) 372
MseI TTAA 2 cut(s) 479, 921
MslI CAYNNNNRTG 4 cut(s) 701, 771, 907, 1161
MspI CCGG 5 cut(s) 85, 373, 392, 501, 1403
MspR9I CCNGG 3 cut(s) 86, 393, 751
MunI CAATTG 1 cut(s) 1281
MvaI CCWGG 1 cut(s) 751
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 5 cut(s) 101, 124, 133, 305, 678
NaeI GCCGGC 1 cut(s) 374
NarI GGCGCC 1 cut(s) 376
NciI CCSGG 2 cut(s) 86, 393
NdeII GATC 2 cut(s) 971, 1334
NgoMIV GCCGGC 1 cut(s) 372
NlaIII CATG 6 cut(s) 491, 605, 741, 1086, 1091, 1125
NlaIV GGNNCC 3 cut(s) 377, 530, 1018
NmeAIII GCCGAG 2 cut(s) 173, 250
NmuCI GTSAC 1 cut(s) 644
NruI TCGCGA 1 cut(s) 35
NspI RCATGY 2 cut(s) 491, 1091
OliI CACNNNNGTG 1 cut(s) 907
PagI TCATGA 1 cut(s) 601
PaqCI CACCTGC 1 cut(s) 1351
PceI AGGCCT 1 cut(s) 274
PciI ACATGT 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 1163
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PdiI GCCGGC 1 cut(s) 374
PfeI GAWTC 6 cut(s) 171, 205, 260, 497, 734, 1262
PflMI CCANNNNNTGG 1 cut(s) 885
PleI GAGTC 6 cut(s) 31, 164, 174, 185, 285, 599
PluTI GGCGCC 1 cut(s) 379
PpsI GAGTC 6 cut(s) 31, 164, 174, 185, 285, 599
PscI ACATGT 1 cut(s) 487
Psp1406I AACGTT 1 cut(s) 1108
Psp6I CCWGG 1 cut(s) 749
PspFI CCCAGC 3 cut(s) 44, 550, 1188
PspGI CCWGG 1 cut(s) 749
PspN4I GGNNCC 3 cut(s) 377, 530, 1018
PspPI GGNCC 1 cut(s) 404
PsrI GAACNNNNNNTAC 2 cut(s) 548, 580
PstNI CAGNNNCTG 3 cut(s) 180, 833, 1007
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 6 cut(s) 365, 388, 523, 530, 566, 1354
RsaNI GTAC 6 cut(s) 364, 387, 522, 529, 565, 1353
RseI CAYNNNNRTG 4 cut(s) 701, 771, 907, 1161
SapI GCTCTTC 1 cut(s) 1163
SaqAI TTAA 2 cut(s) 479, 921
Sau3AI GATC 2 cut(s) 971, 1334
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 6 cut(s) 31, 165, 175, 185, 285, 599
ScrFI CCNGG 3 cut(s) 86, 393, 751
SduI GDGCHC 4 cut(s) 811, 904, 1158, 1175
SfaNI GCATC 3 cut(s) 85, 690, 865
SfoI GGCGCC 1 cut(s) 377
SgrAI CRCCGGYG 1 cut(s) 372
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 4 cut(s) 701, 771, 907, 1161
Sse9I AATT 3 cut(s) 335, 1281, 1409
SseBI AGGCCT 1 cut(s) 274
SspDI GGCGCC 1 cut(s) 375
SspMI CTAG 3 cut(s) 24, 668, 788
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 3 cut(s) 84, 391, 749
TaaI ACNGT 1 cut(s) 930
TaiI ACGT 2 cut(s) 111, 1111
TaqI TCGA 2 cut(s) 169, 258
TasI AATT 3 cut(s) 335, 1281, 1409
TatI WGTACW 2 cut(s) 386, 521
TauI GCSGC 6 cut(s) 8, 11, 130, 253, 1234, 1303
TfiI GAWTC 6 cut(s) 171, 205, 260, 497, 734, 1262
Tru1I TTAA 2 cut(s) 479, 921
Tru9I TTAA 2 cut(s) 479, 921
TscAI CASTG 3 cut(s) 457, 565, 838
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 5 cut(s) 180, 183, 299, 302, 305
Tsp45I GTSAC 1 cut(s) 644
TspGWI ACGGA 2 cut(s) 978, 1254
TspRI CASTG 3 cut(s) 457, 565, 838
Van91I CCANNNNNTGG 1 cut(s) 885
VneI GTGCAC 2 cut(s) 807, 1154
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 1409
XceI RCATGY 2 cut(s) 491, 1091
XmiI GTMKAC 2 cut(s) 916, 1311
XspI CTAG 3 cut(s) 24, 668, 788
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.