Rh6CG454700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005878
Physical Location & Seq
Forward (+)
63055672 .. 63059757
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG454700.1

Sequence Viewer

Length: 1392 bp
ATGGCGGCGGCCACCACCACCTCCACCACTAGAGGCTTCGCGACTCACCCCAGGCGCCTCTCCTTCCTCCATCATCTACCGCATCCGCACCGGAACAATGCTCACACCGCCGACGTGGACGAGCTGAGCAAGCGGCGGAGGCTCACCATCGGCGGAAACCGAGTCGGAGTGAGTCGAATCAGTTGCTGCTGTTCCGACTCGGCGGCTCCGATTCGGAGTAGCTCCCCCGCCGAGAAGAGGAACAAGTGCGACGAGCGGCGGTTCGATTCCAACAAGAGGCCTCCTCACAGAGCTGGACTCAAAGCTGCTGCTGCATTGCCCTTTGCTTCTGAGCAATCTCAATTTGCCTCCAAACAAAACAGCAAGGTGTACCCTCGCCGGCGCCCTCGCTGTACTCCGGGAAATACTGGTCCACAATCTCGTGATACTCCGCCGAAAAGAGATACTGGTATTGCCAGTGAGAAGGAATGGGGTATTAGCTTGTTAAATGAACATGTGAAGGAATCCGGCACTAATGAAGATGGAAGTACTTGGTACCGAGAAAGCGGGGAGGAGCTGGGGAACACTGGGTACAGATGTAGGTGGACAAGGATGGGTGGTGCTTCTCATGACTCCTCTTCAGAGTGGAGAGAAGAGTGGTGGGAGAAAAGTGACTGGACCGGATACAAAGAGCTAGGTGTGGAGAAATCTGGTAGAAATGCTGAAGGGGATTCATGGTGGGAAACCTGGCAAGAGGTTCTTCATCAAGATGAATGGAGTAACCTAGCAAGGATTGAGAGAAGTGCACAGAAACGAGCAAAATCAGGCACTGAAAATGCTGGATGGTCTGAGAAATGGTGGGAGAAATATGATGCCAAAGGCTGGACTGAGAAAGGAGCACACAAATGGGGTAGACTTAATGAACAGTCTTGGTGGGAGAAGTGGGGAGAGCATTATGACGGAAGGGGATCAGTTCTCAAATGGACAGATAAATGGGCAGAGACTGAACTTGGAACCAAATGGGGAGACAAGTGGGAAGAGAAGTTCTTTGAGGGTATAGGTTCACGACAAGGGGAAACATGGCATGTTACTTCCAATGATGAACGTTGGTCAAGGACATGGGGAGAAGAACACATAGGAAATGGTAAAGTGCACAAATATGGGAAGAGCACGACAGGTGAAAGCTGGGATATTGTTGTGGATGAGGAGACCTATTACGAGGCTGTGCCGCATTACGGATGGGCAGATGTTGTAGGCGATTCTACCCAGTTACTATCAATTGAACCTCGTGAAAAGCCGCCTGAGGTCTACCCAAACTTTGACTTTACGAGATCACCACCTGCAATGGGTACACCGCTTCCAGAGAATGAACCACCACCACCTACAGAGGATGAACCACCTCCGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

53.04

Weight (kDa)

6.11

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1327
Acc36I ACCTGC 1 cut(s) 1327
Acc65I GGTACC 1 cut(s) 534
AccB1I GGYRCC 3 cut(s) 54, 381, 534
AccB7I CCANNNNNTGG 1 cut(s) 861
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 892, 1287
AccII CGCG 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 1381
AclI AACGTT 1 cut(s) 1084
AclWI GGATC 1 cut(s) 955
AcoI YGGCCR 1 cut(s) 9
AcuI CTGAAG 2 cut(s) 603, 723
AcyI GRCGYC 2 cut(s) 55, 382
AfaI GTAC 6 cut(s) 371, 394, 529, 536, 572, 1330
AfiI CCNNNNNNNGG 5 cut(s) 237, 276, 861, 1214, 1325
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 1 cut(s) 1262
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 2 cut(s) 50, 725
AloI GAACNNNNNNTCC 2 cut(s) 1007, 1039
AluBI AGCT 8 cut(s) 124, 222, 293, 305, 480, 556, 673, 1164
AluI AGCT 8 cut(s) 124, 222, 293, 305, 480, 556, 673, 1164
Alw21I GWGCWC 4 cut(s) 787, 880, 1134, 1151
Alw26I GTCTC 3 cut(s) 974, 999, 1181
Alw44I GTGCAC 2 cut(s) 783, 1130
AlwI GGATC 1 cut(s) 955
AlwNI CAGNNNCTG 3 cut(s) 186, 809, 983
Aor13HI TCCGGA 1 cut(s) 1381
AoxI GGCC 2 cut(s) 9, 278
ApaLI GTGCAC 2 cut(s) 783, 1130
ApeKI GCWGC 4 cut(s) 186, 305, 308, 311
Asp718I GGTACC 1 cut(s) 534
AspLEI GCGC 2 cut(s) 57, 384
AspS9I GGNCC 2 cut(s) 410, 657
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 4 cut(s) 38, 136, 1169, 1305
AvaII GGWCC 2 cut(s) 410, 657
AxyI CCTNAGG 1 cut(s) 1281
BaeGI GKGCMC 2 cut(s) 787, 1134
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BanI GGYRCC 3 cut(s) 54, 381, 534
BauI CACGAG 2 cut(s) 420, 1266
Bbv12I GWGCWC 4 cut(s) 787, 880, 1134, 1151
BbvI GCAGC 4 cut(s) 173, 292, 295, 298
BccI CCATC 6 cut(s) 78, 155, 515, 586, 816, 1212
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BciT130I CCWGG 2 cut(s) 52, 727
BciVI GTATCC 1 cut(s) 656
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 3 cut(s) 974, 999, 1181
BfaI CTAG 3 cut(s) 30, 674, 764
BfmI CTRYAG 1 cut(s) 1362
BfoI RGCGCY 2 cut(s) 58, 385
BfuAI ACCTGC 1 cut(s) 1327
BfuI GTATCC 1 cut(s) 656
BlpI GCTNAGC 1 cut(s) 125
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 3 cut(s) 52, 399, 727
Bme18I GGWCC 2 cut(s) 410, 657
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 410, 657
BmiI GGNNCC 5 cut(s) 56, 207, 383, 536, 994
BmrFI CCNGG 3 cut(s) 52, 399, 727
BmrI ACTGGG 2 cut(s) 576, 1240
BmsI GCATC 2 cut(s) 91, 841
BmuI ACTGGG 2 cut(s) 576, 1240
BplI GAGNNNNNCTC 4 cut(s) 268, 300, 282, 314
Bpu1102I GCTNAGC 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 399
BsaHI GRCGYC 2 cut(s) 55, 382
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 3 cut(s) 90, 659, 1381
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 276, 861, 1214, 1325
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 6 cut(s) 412, 451, 456, 571, 659, 1246
Bse21I CCTNAGG 1 cut(s) 1281
Bse3DI GCAATG 2 cut(s) 314, 1329
BseAI TCCGGA 1 cut(s) 1381
BseBI CCWGG 2 cut(s) 52, 727
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 6 cut(s) 82, 597, 827, 1186, 1223, 1375
BseLI CCNNNNNNNGG 5 cut(s) 237, 276, 861, 1214, 1325
BseMI GCAATG 2 cut(s) 314, 1329
BseMII CTCAG 5 cut(s) 116, 321, 819, 858, 1272
BseNI ACTGG 6 cut(s) 412, 451, 456, 571, 659, 1246
BseRI GAGGAG 4 cut(s) 273, 566, 604, 1199
BseSI GKGCMC 2 cut(s) 787, 1134
BseXI GCAGC 4 cut(s) 173, 292, 295, 298
BseYI CCCAGC 2 cut(s) 556, 1164
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 2 cut(s) 11, 280
BshNI GGYRCC 3 cut(s) 54, 381, 534
BsiHKAI GWGCWC 4 cut(s) 787, 880, 1134, 1151
BsiSI CCGG 6 cut(s) 91, 379, 398, 507, 660, 1382
BslI CCNNNNNNNGG 5 cut(s) 237, 276, 861, 1214, 1325
BsmAI GTCTC 3 cut(s) 974, 999, 1181
BsnI GGCC 2 cut(s) 11, 280
Bso31I GGTCTC 1 cut(s) 1181
Bsp1286I GDGCHC 4 cut(s) 787, 880, 1134, 1151
Bsp13I TCCGGA 1 cut(s) 1381
Bsp143I GATC 2 cut(s) 947, 1310
Bsp1720I GCTNAGC 1 cut(s) 125
Bsp68I TCGCGA 1 cut(s) 41
BspANI GGCC 2 cut(s) 11, 280
BspCNI CTCAG 5 cut(s) 117, 322, 820, 859, 1273
BspEI TCCGGA 1 cut(s) 1381
BspFNI CGCG 1 cut(s) 41
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 5 cut(s) 56, 207, 383, 536, 994
BspMI ACCTGC 1 cut(s) 1327
BspPI GGATC 1 cut(s) 955
BspQI GCTCTTC 1 cut(s) 1139
BspT107I GGYRCC 3 cut(s) 54, 381, 534
BspTNI GGTCTC 1 cut(s) 1181
BsrBI CCGCTC 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 314, 1329
BsrFI RCCGGY 1 cut(s) 378
BsrI ACTGG 6 cut(s) 412, 451, 456, 571, 659, 1246
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 2 cut(s) 947, 1310
BssNI GRCGYC 2 cut(s) 55, 382
BssSI CACGAG 2 cut(s) 420, 1266
Bst2BI CACGAG 2 cut(s) 420, 1266
Bst2UI CCWGG 2 cut(s) 52, 727
Bst4CI ACNGT 1 cut(s) 906
Bst6I CTCTTC 5 cut(s) 230, 622, 627, 1011, 1139
BstACI GRCGYC 2 cut(s) 55, 382
BstC8I GCNNGC 2 cut(s) 131, 380
BstDEI CTNAG 5 cut(s) 125, 330, 828, 867, 1281
BstF5I GGATG 6 cut(s) 82, 597, 827, 1186, 1223, 1375
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 2 cut(s) 58, 385
BstHHI GCGC 2 cut(s) 57, 384
BstKTI GATC 2 cut(s) 950, 1313
BstMAI GTCTC 3 cut(s) 974, 999, 1181
BstMBI GATC 2 cut(s) 947, 1310
BstMWI GCNNNNNNNGC 4 cut(s) 107, 130, 139, 311
BstNI CCWGG 2 cut(s) 52, 727
BstNSI RCATGY 2 cut(s) 497, 1067
BstSCI CCNGG 3 cut(s) 50, 397, 725
BstSFI CTRYAG 1 cut(s) 1362
BstSLI GKGCMC 2 cut(s) 787, 1134
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 4 cut(s) 173, 292, 295, 298
Bsu36I CCTNAGG 1 cut(s) 1281
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 2 cut(s) 11, 280
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 6 cut(s) 82, 597, 827, 1186, 1223, 1375
BtsIMutI CAGTG 3 cut(s) 463, 564, 807
BtuMI TCGCGA 1 cut(s) 41
BveI ACCTGC 1 cut(s) 1327
Cac8I GCNNGC 2 cut(s) 131, 380
CaiI CAGNNNCTG 3 cut(s) 186, 809, 983
CciI TCATGA 1 cut(s) 607
CfoI GCGC 2 cut(s) 57, 384
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 410, 657
Csp6I GTAC 6 cut(s) 370, 393, 528, 535, 571, 1329
CviAII CATG 6 cut(s) 494, 608, 714, 1059, 1064, 1098
CviQI GTAC 6 cut(s) 370, 393, 528, 535, 571, 1329
DdeI CTNAG 5 cut(s) 125, 330, 828, 867, 1281
DinI GGCGCC 2 cut(s) 56, 383
DpnI GATC 2 cut(s) 949, 1312
DpnII GATC 2 cut(s) 947, 1310
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 5 cut(s) 230, 622, 627, 1011, 1139
EarI CTCTTC 5 cut(s) 230, 622, 627, 1011, 1139
EciI GGCGGA 3 cut(s) 151, 168, 420
Eco147I AGGCCT 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 1181
Eco47I GGWCC 2 cut(s) 410, 657
Eco57I CTGAAG 2 cut(s) 603, 723
Eco81I CCTNAGG 1 cut(s) 1281
EcoRII CCWGG 2 cut(s) 50, 725
EgeI GGCGCC 2 cut(s) 56, 383
EheI GGCGCC 2 cut(s) 56, 383
FaeI CATG 6 cut(s) 497, 611, 717, 1062, 1067, 1101
FalI AAGNNNNNCTT 2 cut(s) 723, 755
FatI CATG 6 cut(s) 493, 607, 713, 1058, 1063, 1097
FauI CCCGC 2 cut(s) 235, 539
FblI GTMKAC 2 cut(s) 892, 1287
FokI GGATG 6 cut(s) 69, 604, 834, 1193, 1230, 1382
FspBI CTAG 3 cut(s) 30, 674, 764
GlaI GCGC 2 cut(s) 56, 383
GsaI CCCAGC 2 cut(s) 560, 1168
HaeII RGCGCY 2 cut(s) 58, 385
HaeIII GGCC 2 cut(s) 11, 280
HapII CCGG 6 cut(s) 91, 379, 398, 507, 660, 1382
HhaI GCGC 2 cut(s) 57, 384
Hin1I GRCGYC 2 cut(s) 55, 382
Hin1II CATG 6 cut(s) 497, 611, 717, 1062, 1067, 1101
Hin6I GCGC 2 cut(s) 55, 382
HinP1I GCGC 2 cut(s) 55, 382
HpaII CCGG 6 cut(s) 91, 379, 398, 507, 660, 1382
HphI GGTGA 4 cut(s) 38, 136, 1169, 1305
Hpy188I TCNGA 7 cut(s) 167, 196, 210, 216, 331, 622, 829
Hpy188III TCNNGA 8 cut(s) 40, 422, 608, 746, 1044, 1268, 1340, 1382
Hpy99I CGWCG 2 cut(s) 116, 254
HpyAV CCTTC 5 cut(s) 73, 457, 493, 698, 936
HpyCH4III ACNGT 1 cut(s) 906
HpyCH4IV ACGT 2 cut(s) 114, 1084
HpyCH4V TGCA 4 cut(s) 314, 785, 1132, 1322
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 130, 139, 311
HpyF3I CTNAG 5 cut(s) 125, 330, 828, 867, 1281
HpySE526I ACGT 2 cut(s) 114, 1084
Hsp92I GRCGYC 2 cut(s) 55, 382
Hsp92II CATG 6 cut(s) 497, 611, 717, 1062, 1067, 1101
HspAI GCGC 2 cut(s) 55, 382
KasI GGCGCC 2 cut(s) 54, 381
Kpn2I TCCGGA 1 cut(s) 1381
KpnI GGTACC 1 cut(s) 538
KroI GCCGGC 1 cut(s) 378
KroNI GCCGGC 1 cut(s) 380
Kzo9I GATC 2 cut(s) 947, 1310
LguI GCTCTTC 1 cut(s) 1139
LmnI GCTCC 4 cut(s) 211, 227, 553, 875
Lsp1109I GCAGC 4 cut(s) 173, 292, 295, 298
LweI GCATC 2 cut(s) 91, 841
MaeI CTAG 3 cut(s) 30, 674, 764
MaeII ACGT 2 cut(s) 114, 1084
MaeIII GTNAC 4 cut(s) 650, 758, 1066, 1248
MalI GATC 2 cut(s) 949, 1312
MbiI CCGCTC 1 cut(s) 256
MboI GATC 2 cut(s) 947, 1310
MboII GAAGA 8 cut(s) 247, 530, 609, 644, 731, 1028, 1118, 1156
MfeI CAATTG 1 cut(s) 1257
MhlI GDGCHC 4 cut(s) 787, 880, 1134, 1151
MluCI AATT 2 cut(s) 341, 1257
Mly113I GGCGCC 2 cut(s) 55, 382
MlyI GAGTC 6 cut(s) 37, 171, 181, 191, 291, 605
MmeI TCCRAC 3 cut(s) 145, 219, 294
MreI CGCCGGCG 1 cut(s) 378
MroI TCCGGA 1 cut(s) 1381
MroNI GCCGGC 1 cut(s) 378
MseI TTAA 3 cut(s) 485, 897, 1390
MslI CAYNNNNRTG 3 cut(s) 747, 883, 1137
MspI CCGG 6 cut(s) 91, 379, 398, 507, 660, 1382
MspR9I CCNGG 3 cut(s) 52, 399, 727
MunI CAATTG 1 cut(s) 1257
MvaI CCWGG 2 cut(s) 52, 727
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 4 cut(s) 107, 130, 139, 311
NaeI GCCGGC 1 cut(s) 380
NarI GGCGCC 2 cut(s) 55, 382
NciI CCSGG 1 cut(s) 399
NdeII GATC 2 cut(s) 947, 1310
NgoMIV GCCGGC 1 cut(s) 378
NlaIII CATG 6 cut(s) 497, 611, 717, 1062, 1067, 1101
NlaIV GGNNCC 5 cut(s) 56, 207, 383, 536, 994
NmeAIII GCCGAG 2 cut(s) 179, 256
NmuCI GTSAC 1 cut(s) 650
NruI TCGCGA 1 cut(s) 41
NspI RCATGY 2 cut(s) 497, 1067
PagI TCATGA 1 cut(s) 607
PaqCI CACCTGC 1 cut(s) 1327
PceI AGGCCT 1 cut(s) 280
PciI ACATGT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PdiI GCCGGC 1 cut(s) 380
PfeI GAWTC 6 cut(s) 177, 211, 266, 503, 710, 1238
PflMI CCANNNNNTGG 1 cut(s) 861
PfoI TCCNGGA 1 cut(s) 397
PleI GAGTC 6 cut(s) 37, 170, 180, 191, 291, 605
PluTI GGCGCC 2 cut(s) 58, 385
PpsI GAGTC 6 cut(s) 37, 170, 180, 191, 291, 605
PscI ACATGT 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 1084
Psp6I CCWGG 2 cut(s) 50, 725
PspFI CCCAGC 2 cut(s) 556, 1164
PspGI CCWGG 2 cut(s) 50, 725
PspN4I GGNNCC 5 cut(s) 56, 207, 383, 536, 994
PspPI GGNCC 2 cut(s) 410, 657
PsrI GAACNNNNNNTAC 2 cut(s) 554, 586
PstNI CAGNNNCTG 3 cut(s) 186, 809, 983
RruI TCGCGA 1 cut(s) 41
RsaI GTAC 6 cut(s) 371, 394, 529, 536, 572, 1330
RsaNI GTAC 6 cut(s) 370, 393, 528, 535, 571, 1329
RseI CAYNNNNRTG 3 cut(s) 747, 883, 1137
SapI GCTCTTC 1 cut(s) 1139
SaqAI TTAA 3 cut(s) 485, 897, 1390
Sau3AI GATC 2 cut(s) 947, 1310
Sau96I GGNCC 2 cut(s) 410, 657
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 6 cut(s) 37, 171, 181, 191, 291, 605
ScrFI CCNGG 3 cut(s) 52, 399, 727
SduI GDGCHC 4 cut(s) 787, 880, 1134, 1151
SfaNI GCATC 2 cut(s) 91, 841
SfcI CTRYAG 1 cut(s) 1362
SfoI GGCGCC 2 cut(s) 56, 383
SgrAI CRCCGGYG 1 cut(s) 378
SinI GGWCC 2 cut(s) 410, 657
SmiMI CAYNNNNRTG 3 cut(s) 747, 883, 1137
Sse9I AATT 2 cut(s) 341, 1257
SseBI AGGCCT 1 cut(s) 280
SspDI GGCGCC 2 cut(s) 54, 381
SspMI CTAG 3 cut(s) 30, 674, 764
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 3 cut(s) 50, 397, 725
TaaI ACNGT 1 cut(s) 906
TaiI ACGT 2 cut(s) 117, 1087
TaqI TCGA 2 cut(s) 175, 264
TasI AATT 2 cut(s) 341, 1257
TatI WGTACW 2 cut(s) 392, 527
TauI GCSGC 7 cut(s) 8, 11, 136, 206, 259, 1210, 1279
TfiI GAWTC 6 cut(s) 177, 211, 266, 503, 710, 1238
Tru1I TTAA 3 cut(s) 485, 897, 1390
Tru9I TTAA 3 cut(s) 485, 897, 1390
TscAI CASTG 3 cut(s) 463, 571, 814
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 4 cut(s) 186, 305, 308, 311
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 9 cut(s) 504, 531, 702, 731, 765, 915, 1095, 1362, 1386
TspGWI ACGGA 2 cut(s) 954, 1230
TspRI CASTG 3 cut(s) 463, 571, 814
Van91I CCANNNNNTGG 1 cut(s) 861
VneI GTGCAC 2 cut(s) 783, 1130
VpaK11BI GGWCC 2 cut(s) 410, 657
XceI RCATGY 2 cut(s) 497, 1067
XmiI GTMKAC 2 cut(s) 892, 1287
XspI CTAG 3 cut(s) 30, 674, 764
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.