FvH4_2g38150

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003258
Physical Location & Seq
Reverse (-)
27634249 .. 27636738
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38150.t1

Sequence Viewer

Length: 963 bp
ATGAGAAGGATTAACGGACTCGCTTTCCTCGGCCGCACCGCCGCTGGTCTCGGCGGCGCCACCAGAGTCGCCGGCGCAACTCCACAGCGGCTGGTTCAAGCGGCGACGATAAGCTCGTGCTCGAATTCGAATCCACTCTGGTTCAGTCAAAGCTCCGGCGATCGCTTTGGCCTATCAAAACCGTCTACGGCTGCCGGAGGCGCGATTTGGTTCTCTACTACTGCGGCTCAGGAGATGCACTCCCACGTCTCCGGCGATAAGTTCGCTCCGAACGACGTCGTTCTGTACCAGTATGAAGCCTGTCCTTTCTGCAACAAGGTTCGAGCATTTTTGGACTATAAGAAGATTCCATACAAAGTTATGGAGGTTAATCCCATGAGCAAAAAGGAGATCAAATGGTCTGATTACAAGAAGGTGCCCATACTGAAGGTTGATGATGAACAGATGGTTGATTCTTCAGATATAATTGATAAGCTATCCCAGAGAATCAATCCGGAGAACAATGTTGTAGATAATGAGGAAGAAAAGAAATGGCGCCACTGGGTGGATAATCACTTGGTGCATGTTTTATCACCAAACATATATCGAACTCCTTCAGAAGCTCTCGAGTCATTTGATTATATCACCAGTCACGGGAATTTCAGTTTATCTGAAAGGCTAGTAGCCAAGTATGGTGGAGCTGCAGCAATGTATTTTGTATCAAAAAAATTGAAGAAGAGACACAATATCACCGATGCTCGTGCAGCTTTATATGGAGCTGCAGAAACTTGGGTGGATGCTCTGAAGGGCCGGCAGTTTCTTGGTGGGTCAACTCCTAACTTGGCTGATCTTGCTGTATTTGGTGTTTTGAGGCCGATTCGGCACCTTAAATCTGGCAAAGAAATGGTGGAGAACACCCGGATCGGTGAATGGTATAGTCGAATGGAGAGTGCAGTGGGAGAGTCTGCTAGAATCAATGCCTAA

Protein Analysis

321

Amino Acids

35.6

Weight (kDa)

9.24

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 93 - 147 2.8e-09 Glutaredoxin
GST_N_3 PF13417 95 - 163 1.1e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 100 - 160 1e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 102 - 190 2.4e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 4 cut(s) 56, 415, 534, 861
AccB7I CCANNNNNTGG 1 cut(s) 544
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 203
AccIII TCCGGA 1 cut(s) 493
AciI CCGC 7 cut(s) 34, 39, 42, 54, 88, 101, 224
AclWI GGATC 1 cut(s) 908
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 2 cut(s) 124, 637
AcuI CTGAAG 4 cut(s) 441, 446, 579, 803
AcyI GRCGYC 3 cut(s) 57, 276, 535
AdeI CACNNNGTG 2 cut(s) 544, 559
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 544, 633
AgsI TTSAA 2 cut(s) 98, 712
AjiI CACGTC 1 cut(s) 247
AluBI AGCT 7 cut(s) 114, 153, 475, 602, 680, 746, 758
AluI AGCT 7 cut(s) 114, 153, 475, 602, 680, 746, 758
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 3 cut(s) 53, 253, 712
AlwI GGATC 1 cut(s) 908
AlwNI CAGNNNCTG 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 493
AoxI GGCC 4 cut(s) 31, 169, 787, 851
ApeKI GCWGC 5 cut(s) 191, 680, 683, 743, 758
ApoI RAATTY 2 cut(s) 124, 637
AsiSI GCGATCGC 1 cut(s) 163
Asp700I GAANNNNTTC 1 cut(s) 592
AspLEI GCGC 4 cut(s) 59, 77, 203, 537
AspS9I GGNCC 1 cut(s) 787
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 4 cut(s) 564, 616, 721, 917
AsuII TTCGAA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 420
BanI GGYRCC 4 cut(s) 56, 415, 534, 861
BarI GAAGNNNNNNTAC 2 cut(s) 335, 367
BauI CACGAG 2 cut(s) 115, 738
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 5 cut(s) 178, 667, 695, 745, 755
BccI CCATC 1 cut(s) 439
BceAI ACGGC 1 cut(s) 204
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 3 cut(s) 53, 253, 712
BfaI CTAG 2 cut(s) 659, 948
BfmI CTRYAG 2 cut(s) 681, 759
BfoI RGCGCY 2 cut(s) 60, 538
BglI GCCNNNNNGGC 1 cut(s) 859
Bme1390I CCNGG 1 cut(s) 898
BmeT110I CYCGRG 1 cut(s) 605
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 787
BmiI GGNNCC 4 cut(s) 58, 417, 536, 863
BmrFI CCNGG 1 cut(s) 898
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 3 cut(s) 225, 724, 766
BmuI ACTGGG 1 cut(s) 550
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 898
BsaHI GRCGYC 3 cut(s) 57, 276, 535
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 2 cut(s) 544, 633
Bse118I RCCGGY 2 cut(s) 71, 789
Bse1I ACTGG 3 cut(s) 289, 545, 627
Bse3DI GCAATG 1 cut(s) 693
BseAI TCCGGA 1 cut(s) 493
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 1 cut(s) 781
BseLI CCNNNNNNNGG 2 cut(s) 544, 633
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 3 cut(s) 289, 545, 627
BseSI GKGCMC 1 cut(s) 420
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 5 cut(s) 178, 667, 695, 745, 755
BsgI GTGCAG 2 cut(s) 762, 951
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 2 cut(s) 34, 163
BshFI GGCC 4 cut(s) 33, 171, 789, 853
BshNI GGYRCC 4 cut(s) 56, 415, 534, 861
BsiEI CGRYCG 2 cut(s) 34, 163
BsiHKAI GWGCWC 1 cut(s) 122
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 7 cut(s) 72, 156, 195, 252, 494, 790, 898
BslI CCNNNNNNNGG 2 cut(s) 544, 633
BsmAI GTCTC 3 cut(s) 53, 253, 712
BsmBI CGTCTC 1 cut(s) 253
BsnI GGCC 4 cut(s) 33, 171, 789, 853
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 605
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 122, 420
Bsp13I TCCGGA 1 cut(s) 493
Bsp143I GATC 4 cut(s) 160, 390, 826, 900
BspACI CCGC 7 cut(s) 34, 39, 42, 54, 88, 101, 224
BspANI GGCC 4 cut(s) 33, 171, 789, 853
BspCNI CTCAG 1 cut(s) 241
BspEI TCCGGA 1 cut(s) 493
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 4 cut(s) 58, 417, 536, 863
BspMAI CTGCAG 2 cut(s) 685, 763
BspPI GGATC 1 cut(s) 908
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 4 cut(s) 56, 415, 534, 861
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 693
BsrFI RCCGGY 2 cut(s) 71, 789
BsrI ACTGG 3 cut(s) 289, 545, 627
BssAI RCCGGY 2 cut(s) 71, 789
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 4 cut(s) 160, 390, 826, 900
BssNI GRCGYC 3 cut(s) 57, 276, 535
BssSI CACGAG 2 cut(s) 115, 738
Bst2BI CACGAG 2 cut(s) 115, 738
Bst4CI ACNGT 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 710
BstACI GRCGYC 3 cut(s) 57, 276, 535
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 73, 791
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 781
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 2 cut(s) 60, 538
BstHHI GCGC 4 cut(s) 59, 77, 203, 537
BstKTI GATC 4 cut(s) 163, 393, 829, 903
BstMAI GTCTC 3 cut(s) 53, 253, 712
BstMBI GATC 4 cut(s) 160, 390, 826, 900
BstMCI CGRYCG 2 cut(s) 34, 163
BstMWI GCNNNNNNNGC 4 cut(s) 200, 743, 830, 859
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 896
BstSFI CTRYAG 2 cut(s) 681, 759
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 5 cut(s) 178, 667, 695, 745, 755
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 4 cut(s) 33, 171, 789, 853
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 1 cut(s) 781
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 2 cut(s) 538, 939
Cac8I GCNNGC 2 cut(s) 73, 791
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 4 cut(s) 59, 77, 203, 537
Cfr10I RCCGGY 2 cut(s) 71, 789
Cfr13I GGNCC 1 cut(s) 787
Csp6I GTAC 1 cut(s) 286
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
CviAII CATG 2 cut(s) 376, 563
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 1 cut(s) 228
DinI GGCGCC 2 cut(s) 58, 536
DpnI GATC 4 cut(s) 162, 392, 828, 902
DpnII GATC 4 cut(s) 160, 390, 826, 900
DraIII CACNNNGTG 2 cut(s) 544, 559
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 710
EarI CTCTTC 1 cut(s) 710
EclXI CGGCCG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 4 cut(s) 441, 446, 579, 803
Eco88I CYCGRG 1 cut(s) 605
EcoRI GAATTC 1 cut(s) 124
EgeI GGCGCC 2 cut(s) 58, 536
EheI GGCGCC 2 cut(s) 58, 536
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 2 cut(s) 379, 566
FatI CATG 2 cut(s) 375, 562
FblI GTMKAC 1 cut(s) 185
FokI GGATG 1 cut(s) 788
FspBI CTAG 2 cut(s) 659, 948
GlaI GCGC 4 cut(s) 58, 76, 202, 536
HaeII RGCGCY 2 cut(s) 60, 538
HaeIII GGCC 4 cut(s) 33, 171, 789, 853
HapII CCGG 7 cut(s) 72, 156, 195, 252, 494, 790, 898
HhaI GCGC 4 cut(s) 59, 77, 203, 537
Hin1I GRCGYC 3 cut(s) 57, 276, 535
Hin1II CATG 2 cut(s) 379, 566
Hin6I GCGC 4 cut(s) 57, 75, 201, 535
HinP1I GCGC 4 cut(s) 57, 75, 201, 535
HincII GTYRAC 1 cut(s) 810
HindII GTYRAC 1 cut(s) 810
HpaII CCGG 7 cut(s) 72, 156, 195, 252, 494, 790, 898
HphI GGTGA 4 cut(s) 564, 616, 721, 917
Hpy166II GTNNAC 2 cut(s) 186, 810
Hpy188I TCNGA 6 cut(s) 270, 403, 460, 598, 652, 783
Hpy188III TCNNGA 3 cut(s) 230, 494, 605
Hpy8I GTNNAC 2 cut(s) 186, 810
Hpy99I CGWCG 3 cut(s) 109, 278, 281
HpyAV CCTTC 4 cut(s) 406, 421, 603, 778
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4IV ACGT 2 cut(s) 246, 276
HpyCH4V TGCA 7 cut(s) 238, 312, 562, 683, 743, 761, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 743, 830, 859
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 2 cut(s) 246, 276
Hsp92I GRCGYC 3 cut(s) 57, 276, 535
Hsp92II CATG 2 cut(s) 379, 566
HspAI GCGC 4 cut(s) 57, 75, 201, 535
KasI GGCGCC 2 cut(s) 56, 534
Kpn2I TCCGGA 1 cut(s) 493
KroI GCCGGC 2 cut(s) 71, 789
KroNI GCCGGC 2 cut(s) 73, 791
Kzo9I GATC 4 cut(s) 160, 390, 826, 900
LmnI GCTCC 4 cut(s) 158, 271, 677, 755
Lsp1109I GCAGC 5 cut(s) 178, 667, 695, 745, 755
LweI GCATC 3 cut(s) 225, 724, 766
MaeI CTAG 2 cut(s) 659, 948
MaeII ACGT 2 cut(s) 246, 276
MaeIII GTNAC 1 cut(s) 629
MalI GATC 4 cut(s) 162, 392, 828, 902
MboI GATC 4 cut(s) 160, 390, 826, 900
MboII GAAGA 5 cut(s) 355, 447, 533, 724, 727
MhlI GDGCHC 2 cut(s) 122, 420
MluCI AATT 4 cut(s) 124, 465, 637, 707
Mly113I GGCGCC 2 cut(s) 57, 535
MlyI GAGTC 4 cut(s) 12, 75, 617, 950
MnlI CCTC 5 cut(s) 38, 191, 358, 511, 843
MreI CGCCGGCG 1 cut(s) 71
MroI TCCGGA 1 cut(s) 493
MroNI GCCGGC 2 cut(s) 71, 789
MroXI GAANNNNTTC 1 cut(s) 592
MseI TTAA 3 cut(s) 12, 369, 867
MspA1I CMGCKG 2 cut(s) 44, 88
MspI CCGG 7 cut(s) 72, 156, 195, 252, 494, 790, 898
MspR9I CCNGG 1 cut(s) 898
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 200, 743, 830, 859
NaeI GCCGGC 2 cut(s) 73, 791
NarI GGCGCC 2 cut(s) 57, 535
NciI CCSGG 1 cut(s) 898
NdeII GATC 4 cut(s) 160, 390, 826, 900
NgoMIV GCCGGC 2 cut(s) 71, 789
NlaIII CATG 2 cut(s) 379, 566
NlaIV GGNNCC 4 cut(s) 58, 417, 536, 863
NmeAIII GCCGAG 2 cut(s) 9, 30
NmuCI GTSAC 1 cut(s) 629
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 128
PaeR7I CTCGAG 1 cut(s) 605
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 252, 270
PdiI GCCGGC 2 cut(s) 73, 791
PdmI GAANNNNTTC 1 cut(s) 592
PfeI GAWTC 6 cut(s) 130, 346, 452, 486, 856, 951
PflMI CCANNNNNTGG 1 cut(s) 544
Ple19I CGATCG 1 cut(s) 163
PleI GAGTC 4 cut(s) 12, 74, 616, 949
PluTI GGCGCC 2 cut(s) 60, 538
PpsI GAGTC 4 cut(s) 12, 74, 616, 949
PspN4I GGNNCC 4 cut(s) 58, 417, 536, 863
PspPI GGNCC 1 cut(s) 787
PstI CTGCAG 2 cut(s) 685, 763
PstNI CAGNNNCTG 1 cut(s) 91
PvuI CGATCG 1 cut(s) 163
RgaI GCGATCGC 1 cut(s) 163
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SaqAI TTAA 3 cut(s) 12, 369, 867
Sau3AI GATC 4 cut(s) 160, 390, 826, 900
Sau96I GGNCC 1 cut(s) 787
SchI GAGTC 4 cut(s) 12, 75, 617, 950
ScrFI CCNGG 1 cut(s) 898
SduI GDGCHC 2 cut(s) 122, 420
SfaAI GCGATCGC 1 cut(s) 163
SfaNI GCATC 3 cut(s) 225, 724, 766
SfcI CTRYAG 2 cut(s) 681, 759
SfoI GGCGCC 2 cut(s) 58, 536
Sfr274I CTCGAG 1 cut(s) 605
SfuI TTCGAA 1 cut(s) 128
SgfI GCGATCGC 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 605
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
Sse9I AATT 4 cut(s) 124, 465, 637, 707
SsiI CCGC 7 cut(s) 34, 39, 42, 54, 88, 101, 224
SspDI GGCGCC 2 cut(s) 56, 534
SspMI CTAG 2 cut(s) 659, 948
StyD4I CCNGG 1 cut(s) 896
TaaI ACNGT 1 cut(s) 183
TaiI ACGT 2 cut(s) 249, 279
TaqI TCGA 6 cut(s) 122, 128, 322, 586, 606, 919
TasI AATT 4 cut(s) 124, 465, 637, 707
TauI GCSGC 6 cut(s) 36, 44, 57, 91, 104, 227
TfiI GAWTC 6 cut(s) 130, 346, 452, 486, 856, 951
Tru1I TTAA 3 cut(s) 12, 369, 867
Tru9I TTAA 3 cut(s) 12, 369, 867
TscAI CASTG 2 cut(s) 545, 939
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 5 cut(s) 191, 680, 683, 743, 758
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 2 cut(s) 309, 453
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 2 cut(s) 545, 939
Van91I CCANNNNNTGG 1 cut(s) 544
XapI RAATTY 2 cut(s) 124, 637
XceI RCATGY 1 cut(s) 566
XhoI CTCGAG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 592
XspI CTAG 2 cut(s) 659, 948
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.