pycom08g00850

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003258
Physical Location & Seq
Reverse (-)
661714 .. 663672
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00850.1

Sequence Viewer

Length: 975 bp
ATGAGAAGGATTAGCGGAATCGCTTTGCTCACCAGAGCCGCCGGTTCTATGGGCGGCGCCACCACAGCCACCTCCGCAACTCAGCACCGTCTGGTTCAAGCAGCGATGTTGAGCACGTGCTCGAATTCCAATCCGCTGTGGTTCACACACAGACTCGGCGATCGTTTCGGCCTATCGAGACCCTCCACTGCGGCTCCCTGTGCCGGAGGGCACGTGTGCTTCTCTACAAACGCCGCCGCTTCTCTGGCTCAGGAGCCGCACGCCCATGAAAAGTTCCTCTCTAAAGACGTCGAGCTCTACCAGTACGAAGCTTGCCCTTTCTGCAACAAGGTTAGAGCATTCTTGGATTACTATAACATTCCGTACAAAGTTGTGGAGGTTAACCCCATTAGCAAAAAGGAGATTAATTGGTCCGATTACAAGAAGGTGCCGATCCTGAAAGTCGACGGCGAACAGATGGTTGACTCTTCAGATATAATTGATAAGCTGTACCTAAGGATCAATCACGAGAACCCCGTGTTAAATGGTGAAGAAGAAATGAAGTGGCGCAGGTGGGTAGATAATCACTTGGTACATGTTTTATCACCAAACATATATAGAACTGTTTCAGAGGCTCTCGAGTCGTTTGACTACATCACCAGTCATGGAAACTTTAGTTTGTACGAAAGGATGGTAGCGAAGTATACCGGAGCTGCTGCTATGTATTTTGTGTCAAAGAAACTGAAGAAGAGACACAATATTACTGATGAACGTGCATCCTTGTATGGAGCTGCAGAAACATGGGTTGATGCTCTCAATGGCAGACAATTTCTTGGTGGATCAAATCCTAGTTTAGCCGATCTCGCTGTATTTGGTGTTTTGAGGCCCATTCGACACCTTAAATCCGGCAAAGATATGGTGGAGAACACGCGGATTGGTGAATGGTATGCTCGAATGGAAAGTGCAGTGGGAGAGTCTGCTAGAGTCAAGGCCTAA

Protein Analysis

325

Amino Acids

36.3

Weight (kDa)

8.94

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 97 - 151 1.1e-09 Glutaredoxin
GST_N_3 PF13417 99 - 164 1.7e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 104 - 163 1.3e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 106 - 194 7.5e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 540
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 540
AccB1I GGYRCC 2 cut(s) 56, 427
AccI GTMKAC 2 cut(s) 444, 683
AccII CGCG 1 cut(s) 910
AclWI GGATC 3 cut(s) 427, 506, 826
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 2 cut(s) 453, 743
AcvI CACGTG 2 cut(s) 117, 214
AcyI GRCGYC 2 cut(s) 57, 288
AfaI GTAC 5 cut(s) 305, 365, 491, 573, 662
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 2 cut(s) 213, 574
AgsI TTSAA 1 cut(s) 98
AluBI AGCT 5 cut(s) 295, 311, 487, 692, 770
AluI AGCT 5 cut(s) 295, 311, 487, 692, 770
Alw21I GWGCWC 3 cut(s) 116, 122, 297
Alw26I GTCTC 2 cut(s) 172, 724
AlwI GGATC 3 cut(s) 427, 506, 826
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 3 cut(s) 169, 863, 969
ApeKI GCWGC 4 cut(s) 101, 692, 695, 770
ApoI RAATTY 1 cut(s) 124
AseI ATTAAT 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 59, 549
AspS9I GGNCC 2 cut(s) 411, 864
AsuHPI GGTGA 5 cut(s) 22, 539, 576, 628, 929
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 411
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 2 cut(s) 56, 427
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 506
BbrPI CACGTG 2 cut(s) 117, 214
Bbv12I GWGCWC 3 cut(s) 116, 122, 297
BbvI GCAGC 4 cut(s) 113, 679, 682, 757
BccI CCATC 2 cut(s) 451, 664
BceAI ACGGC 1 cut(s) 463
BcoDI GTCTC 2 cut(s) 172, 724
BfaI CTAG 2 cut(s) 828, 960
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 540
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 2 cut(s) 411, 864
BmiI GGNNCC 4 cut(s) 58, 195, 255, 429
BmsI GCATC 2 cut(s) 764, 778
Bpu10I CCTNAGC 1 cut(s) 249
BsaAI YACGTR 2 cut(s) 117, 214
BsaHI GRCGYC 2 cut(s) 57, 288
BsaI GGTCTC 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 4 cut(s) 178, 198, 208, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 301, 639
Bse21I CCTNAGG 1 cut(s) 494
BseGI GGATG 2 cut(s) 675, 755
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 2 cut(s) 95, 263
BseNI ACTGG 2 cut(s) 301, 639
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 4 cut(s) 113, 679, 682, 757
BsgI GTGCAG 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 910
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 3 cut(s) 171, 865, 971
BshNI GGYRCC 2 cut(s) 56, 427
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 3 cut(s) 116, 122, 297
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 4 cut(s) 42, 204, 687, 885
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 172, 724
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 3 cut(s) 171, 865, 971
Bso31I GGTCTC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 4 cut(s) 116, 122, 213, 297
Bsp143I GATC 5 cut(s) 160, 432, 498, 818, 838
BspANI GGCC 3 cut(s) 171, 865, 971
BspCNI CTCAG 2 cut(s) 94, 262
BspFNI CGCG 1 cut(s) 910
BspLI GGNNCC 4 cut(s) 58, 195, 255, 429
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 3 cut(s) 427, 506, 826
BspT107I GGYRCC 2 cut(s) 56, 427
BspTNI GGTCTC 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 2 cut(s) 301, 639
BssAI RCCGGY 1 cut(s) 41
BssMI GATC 5 cut(s) 160, 432, 498, 818, 838
BssNAI GTATAC 1 cut(s) 684
BssNI GRCGYC 2 cut(s) 57, 288
BssSI CACGAG 1 cut(s) 506
Bst1107I GTATAC 1 cut(s) 684
Bst2BI CACGAG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 89, 604
Bst6I CTCTTC 2 cut(s) 472, 722
BstACI GRCGYC 2 cut(s) 57, 288
BstBAI YACGTR 2 cut(s) 117, 214
BstC8I GCNNGC 2 cut(s) 261, 313
BstDEI CTNAG 3 cut(s) 81, 249, 494
BstF5I GGATG 2 cut(s) 675, 755
BstFNI CGCG 1 cut(s) 910
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 549
BstKTI GATC 5 cut(s) 163, 435, 501, 821, 841
BstMAI GTCTC 2 cut(s) 172, 724
BstMBI GATC 5 cut(s) 160, 432, 498, 818, 838
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 842
BstNSI RCATGY 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 1 cut(s) 910
BstV1I GCAGC 4 cut(s) 113, 679, 682, 757
BstZ17I GTATAC 1 cut(s) 684
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 3 cut(s) 171, 865, 971
BtgZI GCGATG 1 cut(s) 119
BtsCI GGATG 2 cut(s) 675, 755
BtsI GCAGTG 2 cut(s) 186, 951
BtsIMutI CAGTG 2 cut(s) 186, 951
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 2 cut(s) 261, 313
CfoI GCGC 2 cut(s) 59, 549
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 411, 864
Csp6I GTAC 5 cut(s) 304, 364, 490, 572, 661
CviAII CATG 4 cut(s) 266, 575, 644, 780
CviQI GTAC 5 cut(s) 304, 364, 490, 572, 661
DdeI CTNAG 3 cut(s) 81, 249, 494
DinI GGCGCC 1 cut(s) 58
DpnI GATC 5 cut(s) 162, 434, 500, 820, 840
DpnII GATC 5 cut(s) 160, 432, 498, 818, 838
Eam1104I CTCTTC 2 cut(s) 472, 722
EarI CTCTTC 2 cut(s) 472, 722
Ecl136II GAGCTC 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 971
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 453, 743
Eco72I CACGTG 2 cut(s) 117, 214
Eco81I CCTNAGG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 4 cut(s) 269, 578, 647, 783
FatI CATG 4 cut(s) 265, 574, 643, 779
FblI GTMKAC 2 cut(s) 444, 683
FokI GGATG 2 cut(s) 682, 742
FriOI GRGCYC 1 cut(s) 297
FspBI CTAG 2 cut(s) 828, 960
GlaI GCGC 2 cut(s) 58, 548
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 171, 865, 971
HapII CCGG 4 cut(s) 42, 204, 687, 885
HhaI GCGC 2 cut(s) 59, 549
Hin1I GRCGYC 2 cut(s) 57, 288
Hin1II CATG 4 cut(s) 269, 578, 647, 783
Hin6I GCGC 2 cut(s) 57, 547
HinP1I GCGC 2 cut(s) 57, 547
HincII GTYRAC 3 cut(s) 382, 445, 463
HindII GTYRAC 3 cut(s) 382, 445, 463
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 6 cut(s) 18, 153, 464, 620, 953, 963
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 4 cut(s) 42, 204, 687, 885
HphI GGTGA 5 cut(s) 22, 539, 576, 628, 929
Hpy166II GTNNAC 5 cut(s) 144, 382, 445, 463, 684
Hpy188I TCNGA 3 cut(s) 415, 472, 610
Hpy188III TCNNGA 5 cut(s) 177, 251, 436, 506, 617
Hpy8I GTNNAC 5 cut(s) 144, 382, 445, 463, 684
Hpy99I CGWCG 2 cut(s) 293, 449
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 89, 604
HpyCH4IV ACGT 4 cut(s) 116, 213, 288, 751
HpyCH4V TGCA 4 cut(s) 324, 755, 773, 944
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 842
HpyF3I CTNAG 3 cut(s) 81, 249, 494
HpySE526I ACGT 4 cut(s) 116, 213, 288, 751
Hsp92I GRCGYC 2 cut(s) 57, 288
Hsp92II CATG 4 cut(s) 269, 578, 647, 783
HspAI GCGC 2 cut(s) 57, 547
KasI GGCGCC 1 cut(s) 56
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 5 cut(s) 160, 432, 498, 818, 838
LmnI GCTCC 4 cut(s) 199, 253, 689, 767
Lsp1109I GCAGC 4 cut(s) 113, 679, 682, 757
LweI GCATC 2 cut(s) 764, 778
MaeI CTAG 2 cut(s) 828, 960
MaeII ACGT 4 cut(s) 116, 213, 288, 751
MalI GATC 5 cut(s) 162, 434, 500, 820, 840
MboI GATC 5 cut(s) 160, 432, 498, 818, 838
MboII GAAGA 5 cut(s) 459, 542, 545, 736, 739
MhlI GDGCHC 4 cut(s) 116, 122, 213, 297
MluCI AATT 4 cut(s) 124, 406, 477, 806
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 5 cut(s) 147, 458, 629, 962, 972
MnlI CCTC 7 cut(s) 82, 193, 200, 287, 370, 604, 855
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 381, 405, 521, 879
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 4 cut(s) 42, 204, 687, 885
Mva1269I GAATGC 1 cut(s) 338
MvnI CGCG 1 cut(s) 910
MwoI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 842
NarI GGCGCC 1 cut(s) 57
NdeII GATC 5 cut(s) 160, 432, 498, 818, 838
NlaIII CATG 4 cut(s) 269, 578, 647, 783
NlaIV GGNNCC 4 cut(s) 58, 195, 255, 429
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 617
PaqCI CACCTGC 1 cut(s) 540
PceI AGGCCT 1 cut(s) 971
PciI ACATGT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PctI GAATGC 1 cut(s) 338
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 18
Ple19I CGATCG 1 cut(s) 163
PleI GAGTC 5 cut(s) 147, 458, 628, 961, 971
PluTI GGCGCC 1 cut(s) 60
PmaCI CACGTG 2 cut(s) 117, 214
PmlI CACGTG 2 cut(s) 117, 214
PpsI GAGTC 5 cut(s) 147, 458, 628, 961, 971
Ppu21I YACGTR 2 cut(s) 117, 214
PscI ACATGT 1 cut(s) 574
PshBI ATTAAT 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 297
PspCI CACGTG 2 cut(s) 117, 214
PspN4I GGNNCC 4 cut(s) 58, 195, 255, 429
PspPI GGNCC 2 cut(s) 411, 864
PstI CTGCAG 1 cut(s) 775
PvuI CGATCG 1 cut(s) 163
RsaI GTAC 5 cut(s) 305, 365, 491, 573, 662
RsaNI GTAC 5 cut(s) 304, 364, 490, 572, 661
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 297
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 4 cut(s) 381, 405, 521, 879
Sau3AI GATC 5 cut(s) 160, 432, 498, 818, 838
Sau96I GGNCC 2 cut(s) 411, 864
SchI GAGTC 5 cut(s) 147, 458, 629, 962, 972
SduI GDGCHC 4 cut(s) 116, 122, 213, 297
SfaNI GCATC 2 cut(s) 764, 778
SfcI CTRYAG 1 cut(s) 771
SfoI GGCGCC 1 cut(s) 58
Sfr274I CTCGAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 411
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 4 cut(s) 124, 406, 477, 806
SseBI AGGCCT 1 cut(s) 971
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 1 cut(s) 739
SspMI CTAG 2 cut(s) 828, 960
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 1 cut(s) 971
TaaI ACNGT 2 cut(s) 89, 604
TaiI ACGT 4 cut(s) 119, 216, 291, 754
TaqI TCGA 7 cut(s) 122, 176, 291, 444, 618, 871, 931
TasI AATT 4 cut(s) 124, 406, 477, 806
TauI GCSGC 6 cut(s) 41, 57, 194, 236, 239, 259
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 4 cut(s) 381, 405, 521, 879
Tru9I TTAA 4 cut(s) 381, 405, 521, 879
TscAI CASTG 2 cut(s) 193, 951
TseI GCWGC 4 cut(s) 101, 692, 695, 770
TspDTI ATGAA 3 cut(s) 282, 554, 762
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 2 cut(s) 193, 951
VpaK11BI GGWCC 1 cut(s) 411
VspI ATTAAT 1 cut(s) 405
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 617
XmiI GTMKAC 2 cut(s) 444, 683
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 2 cut(s) 828, 960
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.