Rh7BG014500

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003258
Physical Location & Seq
Forward (+)
927055 .. 931380
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG014500.1

Sequence Viewer

Length: 999 bp
ATGAGAAGGGCTTCCACTCTCGCCTCCTTCCCCATCCTCTCGCGATCCATCGCCACCCTCCACGGCGGCGCAACCGCCTCGTCACCCTCCCACCGCCTTCTCCAGGTAGCTCTCTACAGCTCCGCCGGCGCCAGCTCTCATGGTCGCCGTCCCTGGTTCGAGTCATTTTCCGGCCGGAAGCTGGCCGTCGGCGTCGCCGGAACTCTGGCTTCGGTGGCTGTTGCCACGTCGCTCGCTCAGGAAGTCCACGCCAAAGAGCCGCCTCCGGCTGAGCTCGTCCCCAAGGAGGTTGTGCTGTACCAGTACGAAGCTTGCCCTTTCTGCAACAAAGTGAAAGCATTCTTGGACTACTATGATATACCGTACAAAATTGTGGAGGTGAACCCACTCAGTAAGAAAGAGATCAAGTTTTCGGAGTACAAGAAGGTTCCGATATTGATGGTGGATGGGGAACAGCTAGTTGATTCATCAGATATAATTGATACCTTGACTGAGAAGATTCTTCCTGACAGAGTACCGCAGAGAGTAGCAGTTTCTACCTCAGAAGAGGATGATGAAGAGAAAAAGTGGCGCAAGTGGGTTGATAATCACCTGGTGCATATGCTATCACCAAACATATACCGAAATACATCTGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTTAGTTACACAGAAAAAATTACAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTGTCTAAGAAACTGAAGAAGAAATATAACATTACTGATGAACGTGCATCCCTGTATGAAGCCGCAGAAACATGGGTTGATGCTTTAGATGGGCGGGAATTCCTTGGGGGGTCAAAACCTAATTTGGCTGATCTTGCTGTTTTTGGGGTGTTAAGACCTATCCGTTATTTGAGGTCTGGTAAAGATATGGTTGAGCACACACGCATAGGTGAATGGTACTCAAGAATGGAGCGTGCTGTAGGAGAGCCTTCAAGGATTAAGGAGTAG

Protein Analysis

332

Amino Acids

37.24

Weight (kDa)

8.28

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 97 - 151 3.9e-10 Glutaredoxin
GST_N_3 PF13417 99 - 165 1.3e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 104 - 165 4.2e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 106 - 202 1.1e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccII CGCG 1 cut(s) 43
AciI CCGC 8 cut(s) 66, 75, 94, 123, 260, 518, 795, 826
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 2 cut(s) 172, 183
AcsI RAATTY 1 cut(s) 830
AcuI CTGAAG 1 cut(s) 767
AcyI GRCGYC 2 cut(s) 129, 192
AdeI CACNNNGTG 1 cut(s) 595
AfaI GTAC 7 cut(s) 299, 305, 365, 419, 516, 728, 950
AfiI CCNNNNNNNGG 3 cut(s) 103, 181, 286
AgsI TTSAA 1 cut(s) 984
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 3 cut(s) 102, 152, 591
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 8 cut(s) 110, 120, 135, 181, 274, 311, 457, 722
AluI AGCT 8 cut(s) 110, 120, 135, 181, 274, 311, 457, 722
Alw21I GWGCWC 2 cut(s) 276, 930
AlwI GGATC 1 cut(s) 39
AoxI GGCC 3 cut(s) 172, 183, 636
ApeKI GCWGC 2 cut(s) 716, 719
ApoI RAATTY 1 cut(s) 830
Asp700I GAANNNNTTC 2 cut(s) 10, 338
AspLEI GCGC 3 cut(s) 71, 131, 573
AspS9I GGNCC 1 cut(s) 637
AsuHPI GGTGA 5 cut(s) 75, 391, 581, 600, 953
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 276, 930
BbvI GCAGC 2 cut(s) 703, 731
BccI CCATC 5 cut(s) 41, 56, 433, 440, 815
BceAI ACGGC 3 cut(s) 79, 132, 170
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 3 cut(s) 104, 154, 593
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 3 cut(s) 115, 717, 969
BfoI RGCGCY 1 cut(s) 132
BisI GCNGC 5 cut(s) 67, 260, 717, 720, 795
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 5 cut(s) 68, 261, 718, 721, 796
Bme1390I CCNGG 3 cut(s) 104, 154, 593
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 2 cut(s) 130, 429
BmrFI CCNGG 3 cut(s) 104, 154, 593
BmsI GCATC 2 cut(s) 788, 802
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 662, 937
BsaHI GRCGYC 2 cut(s) 129, 192
BsaJI CCNNGG 4 cut(s) 61, 152, 282, 835
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 181, 286
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 673
BseBI CCWGG 3 cut(s) 104, 154, 593
BseDI CCNNGG 4 cut(s) 61, 152, 282, 835
BseGI GGATG 4 cut(s) 33, 451, 556, 779
BseLI CCNNNNNNNGG 3 cut(s) 103, 181, 286
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 6 cut(s) 251, 261, 403, 483, 555, 624
BseNI ACTGG 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 703, 731
Bsh1236I CGCG 1 cut(s) 43
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 3 cut(s) 174, 185, 638
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 276, 930
BsiSI CCGG 5 cut(s) 126, 171, 175, 198, 266
BslFI GGGAC 2 cut(s) 135, 263
BslI CCNNNNNNNGG 3 cut(s) 103, 181, 286
BsmFI GGGAC 2 cut(s) 135, 263
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 3 cut(s) 174, 185, 638
Bsp1286I GDGCHC 2 cut(s) 276, 930
Bsp143I GATC 3 cut(s) 44, 402, 862
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp68I TCGCGA 1 cut(s) 43
BspACI CCGC 8 cut(s) 66, 75, 94, 123, 260, 518, 795, 826
BspANI GGCC 3 cut(s) 174, 185, 638
BspCNI CTCAG 6 cut(s) 250, 262, 402, 484, 554, 625
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 130, 429
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 4 cut(s) 61, 152, 282, 835
BssMI GATC 3 cut(s) 44, 402, 862
BssNI GRCGYC 2 cut(s) 129, 192
BssT1I CCWWGG 2 cut(s) 282, 835
Bst2UI CCWGG 3 cut(s) 104, 154, 593
Bst4CI ACNGT 2 cut(s) 363, 700
Bst6I CTCTTC 2 cut(s) 540, 552
BstACI GRCGYC 2 cut(s) 129, 192
BstC8I GCNNGC 6 cut(s) 127, 133, 183, 234, 313, 966
BstDEI CTNAG 7 cut(s) 237, 270, 389, 492, 541, 633, 738
BstDSI CCRYGG 1 cut(s) 61
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 4 cut(s) 33, 451, 556, 779
BstFNI CGCG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 3 cut(s) 71, 131, 573
BstKTI GATC 3 cut(s) 47, 405, 865
BstMBI GATC 3 cut(s) 44, 402, 862
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
BstNI CCWGG 3 cut(s) 104, 154, 593
BstSCI CCNGG 3 cut(s) 102, 152, 591
BstSFI CTRYAG 3 cut(s) 115, 717, 969
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 2 cut(s) 703, 731
BstZI CGGCCG 1 cut(s) 172
BsuRI GGCC 3 cut(s) 174, 185, 638
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 34
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 4 cut(s) 33, 451, 556, 779
BtsIMutI CAGTG 1 cut(s) 705
BtuMI TCGCGA 1 cut(s) 43
Cac8I GCNNGC 6 cut(s) 127, 133, 183, 234, 313, 966
CfoI GCGC 3 cut(s) 71, 131, 573
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 637
CseI GACGC 1 cut(s) 181
CsiI ACCWGGT 1 cut(s) 591
Csp6I GTAC 7 cut(s) 298, 304, 364, 418, 515, 727, 949
CviAII CATG 2 cut(s) 140, 804
CviQI GTAC 7 cut(s) 298, 304, 364, 418, 515, 727, 949
DdeI CTNAG 7 cut(s) 237, 270, 389, 492, 541, 633, 738
DinI GGCGCC 1 cut(s) 130
DpnI GATC 3 cut(s) 46, 404, 864
DpnII GATC 3 cut(s) 44, 402, 862
DraIII CACNNNGTG 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 172, 183
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 540, 552
EarI CTCTTC 2 cut(s) 540, 552
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 274
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 282, 835
Eco24I GRGCYC 1 cut(s) 276
Eco52I CGGCCG 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 3 cut(s) 102, 152, 591
EcoT14I CCWWGG 2 cut(s) 282, 835
EcoT38I GRGCYC 1 cut(s) 276
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 2 cut(s) 282, 835
FaeI CATG 2 cut(s) 143, 807
FaqI GGGAC 2 cut(s) 135, 263
FatI CATG 2 cut(s) 139, 803
FauI CCCGC 1 cut(s) 819
FauNDI CATATG 1 cut(s) 600
Fnu4HI GCNGC 5 cut(s) 67, 260, 717, 720, 795
FokI GGATG 4 cut(s) 20, 458, 563, 766
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 5 cut(s) 67, 260, 717, 720, 795
FspBI CTAG 1 cut(s) 458
GlaI GCGC 3 cut(s) 70, 130, 572
GluI GCNGC 5 cut(s) 67, 260, 717, 720, 795
GsuI CTGGAG 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 3 cut(s) 174, 185, 638
HapII CCGG 5 cut(s) 126, 171, 175, 198, 266
HgaI GACGC 1 cut(s) 181
HhaI GCGC 3 cut(s) 71, 131, 573
Hin1I GRCGYC 2 cut(s) 129, 192
Hin1II CATG 2 cut(s) 143, 807
Hin6I GCGC 3 cut(s) 69, 129, 571
HinP1I GCGC 3 cut(s) 69, 129, 571
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 4 cut(s) 161, 464, 499, 644
HpaII CCGG 5 cut(s) 126, 171, 175, 198, 266
HphI GGTGA 5 cut(s) 75, 391, 581, 600, 953
Hpy166II GTNNAC 2 cut(s) 247, 382
Hpy188I TCNGA 5 cut(s) 415, 432, 472, 544, 634
Hpy188III TCNNGA 4 cut(s) 42, 239, 506, 954
Hpy8I GTNNAC 2 cut(s) 247, 382
Hpy99I CGWCG 3 cut(s) 191, 197, 232
HpyAV CCTTC 4 cut(s) 37, 107, 418, 990
HpyCH4III ACNGT 2 cut(s) 363, 700
HpyCH4IV ACGT 2 cut(s) 227, 775
HpyCH4V TGCA 4 cut(s) 324, 598, 719, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
HpyF3I CTNAG 7 cut(s) 237, 270, 389, 492, 541, 633, 738
HpySE526I ACGT 2 cut(s) 227, 775
Hsp92I GRCGYC 2 cut(s) 129, 192
Hsp92II CATG 2 cut(s) 143, 807
HspAI GCGC 3 cut(s) 69, 129, 571
KasI GGCGCC 1 cut(s) 128
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 44, 402, 862
LmnI GCTCC 2 cut(s) 125, 961
Lsp1109I GCAGC 2 cut(s) 703, 731
LweI GCATC 2 cut(s) 788, 802
MabI ACCWGGT 1 cut(s) 591
MaeI CTAG 1 cut(s) 458
MaeII ACGT 2 cut(s) 227, 775
MaeIII GTNAC 2 cut(s) 81, 680
MalI GATC 3 cut(s) 46, 404, 864
MboI GATC 3 cut(s) 44, 402, 862
MboII GAAGA 6 cut(s) 494, 508, 557, 569, 760, 763
MhlI GDGCHC 2 cut(s) 276, 930
MluCI AATT 6 cut(s) 369, 477, 673, 693, 830, 853
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 2 cut(s) 170, 653
MreI CGCCGGCG 1 cut(s) 125
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 10, 338
MseI TTAA 2 cut(s) 884, 990
MspI CCGG 5 cut(s) 126, 171, 175, 198, 266
MspR9I CCNGG 3 cut(s) 104, 154, 593
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 3 cut(s) 104, 154, 593
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
NaeI GCCGGC 1 cut(s) 127
NarI GGCGCC 1 cut(s) 129
NdeI CATATG 1 cut(s) 600
NdeII GATC 3 cut(s) 44, 402, 862
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 2 cut(s) 143, 807
NlaIV GGNNCC 2 cut(s) 130, 429
NmuCI GTSAC 1 cut(s) 81
NruI TCGCGA 1 cut(s) 43
PctI GAATGC 1 cut(s) 338
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 10, 338
PfeI GAWTC 2 cut(s) 464, 499
PkrI GCNGC 5 cut(s) 68, 261, 718, 721, 796
PleI GAGTC 2 cut(s) 169, 652
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 2 cut(s) 169, 652
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 3 cut(s) 102, 152, 591
PspGI CCWGG 3 cut(s) 102, 152, 591
PspN4I GGNNCC 2 cut(s) 130, 429
PspPI GGNCC 1 cut(s) 637
PstI CTGCAG 1 cut(s) 721
RruI TCGCGA 1 cut(s) 43
RsaI GTAC 7 cut(s) 299, 305, 365, 419, 516, 728, 950
RsaNI GTAC 7 cut(s) 298, 304, 364, 418, 515, 727, 949
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 884, 990
SatI GCNGC 5 cut(s) 67, 260, 717, 720, 795
Sau3AI GATC 3 cut(s) 44, 402, 862
Sau96I GGNCC 1 cut(s) 637
SchI GAGTC 2 cut(s) 170, 653
ScrFI CCNGG 3 cut(s) 104, 154, 593
SduI GDGCHC 2 cut(s) 276, 930
SexAI ACCWGGT 1 cut(s) 591
SfaNI GCATC 2 cut(s) 788, 802
SfcI CTRYAG 3 cut(s) 115, 717, 969
SfoI GGCGCC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 641, 952
SmoI CTYRAG 2 cut(s) 641, 952
Sse9I AATT 6 cut(s) 369, 477, 673, 693, 830, 853
SsiI CCGC 8 cut(s) 66, 75, 94, 123, 260, 518, 795, 826
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 102, 152, 591
StyI CCWWGG 2 cut(s) 282, 835
TaaI ACNGT 2 cut(s) 363, 700
TaiI ACGT 2 cut(s) 230, 778
TaqI TCGA 1 cut(s) 159
TasI AATT 6 cut(s) 369, 477, 673, 693, 830, 853
TatI WGTACW 2 cut(s) 417, 726
TauI GCSGC 3 cut(s) 69, 262, 797
TfiI GAWTC 2 cut(s) 464, 499
Tru1I TTAA 2 cut(s) 884, 990
Tru9I TTAA 2 cut(s) 884, 990
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 2 cut(s) 716, 719
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 4 cut(s) 456, 570, 786, 804
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 1 cut(s) 705
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 830
XmnI GAANNNNTTC 2 cut(s) 10, 338
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.