RLG00000005453

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003258
Physical Location & Seq
Reverse (-)
67729255 .. 67731813
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005453

Sequence Viewer

Length: 993 bp
ATGAGAAGGGCTTCCACTCTCGCCTCCTTCCCCATCCTCTCCCGATCCATTGCCACCCTCCACGGCGGCGCAACCGCCACGTCACCCTCCCACCGCCTTCTCCAGGTAGCTCTCTACAGCTCCGCCGGCGCCGGCTCTCATGGTCGCCGTCTCTGGTTCGAGTCATTTTCAGGCAAGAAGCTGGCCGTCGGCGTCGCCGGAACTCTGGCTTCGGTGGCTGTTGCCACGTCACTCGCTCAGGAAGTCCACGCCAAAGAGCCGCCTCCGGCTGAGCTCGTGCCTAAGGAGGTTGTGCTGTACCAGTACGAAGCTTGCCCTTTCTGCAACAAAGTGAAAGCATTCTTGGACTACTATGATATACCATACAAAATTGTGGAGGTGAACCCACTCAGTAAGAAAGAGATCAAGTTTTCGGAGTATAAGAAGGTTCCGATATTGATGGTGGATGGGGAACAGCTAGTTGATTCATCAGATATAATTGATACCTTGACTGAGAAGATTCTTCCTGACAGAGTACCGCAGAGAGTAGCAGTTTCTACCTCAGATGAGGATGATGAAGAGAAAAAGTGGCGCAGGTGGGTTGATAATCACCTGGTGCATATGTTATCACCAAACATATACCGAAATACATCTGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTCAGTTACACAGAAAAAATTACAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTTTCTAAGAAACTGAAGAAGAAATATAACATTACTGATGAACGTGCATCCCTGTATGAAGCAGCAGAAACATGGGTTGATGCTCTAGATGGGCGGGAATTCCTTGGGGGGTCAAAACCTAATTTGGCTGATCTTGCTGTTTTTGGGGTGTTAAGACCCATCCGTTATTTGAGGTCTGGTAAAGATATGGTTGAGCACACACGCATAGGTGAATGGTACTCAAGAATGGAGCGTGCTGTAGGAGAGCCTTCAAGGATTTAA

Protein Analysis

331

Amino Acids

36.97

Weight (kDa)

8.29

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 97 - 151 3.8e-10 Glutaredoxin
GST_N_3 PF13417 99 - 165 1.2e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 104 - 165 4.2e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 106 - 202 1.5e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 564
AccB1I GGYRCC 1 cut(s) 128
AciI CCGC 7 cut(s) 66, 75, 94, 123, 260, 518, 826
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 830
AcuI CTGAAG 1 cut(s) 767
AcyI GRCGYC 2 cut(s) 129, 192
AdeI CACNNNGTG 1 cut(s) 595
AfaI GTAC 5 cut(s) 299, 305, 516, 728, 950
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 1 cut(s) 984
AjiI CACGTC 2 cut(s) 81, 228
AjnI CCWGG 2 cut(s) 102, 591
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 7 cut(s) 110, 120, 181, 274, 311, 457, 722
AluI AGCT 7 cut(s) 110, 120, 181, 274, 311, 457, 722
Alw21I GWGCWC 2 cut(s) 276, 930
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 39
AoxI GGCC 2 cut(s) 183, 636
ApeKI GCWGC 3 cut(s) 716, 719, 794
ApoI RAATTY 1 cut(s) 830
Asp700I GAANNNNTTC 2 cut(s) 10, 338
AspLEI GCGC 3 cut(s) 71, 131, 573
AspS9I GGNCC 1 cut(s) 637
AsuHPI GGTGA 5 cut(s) 75, 391, 581, 600, 953
AxyI CCTNAGG 1 cut(s) 282
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 276, 930
BbvI GCAGC 3 cut(s) 703, 731, 806
BccI CCATC 5 cut(s) 41, 433, 440, 815, 899
BceAI ACGGC 3 cut(s) 79, 132, 170
BciT130I CCWGG 2 cut(s) 104, 593
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 458, 818
BfmI CTRYAG 3 cut(s) 115, 717, 969
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 564
BisI GCNGC 5 cut(s) 67, 260, 717, 720, 795
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 5 cut(s) 68, 261, 718, 721, 796
Bme1390I CCNGG 2 cut(s) 104, 593
BmgBI CACGTC 2 cut(s) 81, 228
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 2 cut(s) 130, 429
BmrFI CCNGG 2 cut(s) 104, 593
BmsI GCATC 2 cut(s) 788, 802
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 662, 937
BsaHI GRCGYC 2 cut(s) 129, 192
BsaJI CCNNGG 2 cut(s) 61, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 2 cut(s) 125, 131
Bse1I ACTGG 1 cut(s) 301
Bse21I CCTNAGG 1 cut(s) 282
Bse3DI GCAATG 2 cut(s) 48, 673
BseBI CCWGG 2 cut(s) 104, 593
BseDI CCNNGG 2 cut(s) 61, 835
BseGI GGATG 5 cut(s) 33, 451, 556, 779, 891
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMI GCAATG 2 cut(s) 48, 673
BseMII CTCAG 6 cut(s) 251, 261, 403, 483, 555, 624
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 3 cut(s) 703, 731, 806
BshFI GGCC 2 cut(s) 185, 638
BshNI GGYRCC 1 cut(s) 128
BsiHKAI GWGCWC 2 cut(s) 276, 930
BsiSI CCGG 4 cut(s) 126, 132, 198, 266
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 155
BsmBI CGTCTC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 2 cut(s) 185, 638
Bsp1286I GDGCHC 2 cut(s) 276, 930
Bsp143I GATC 3 cut(s) 44, 402, 862
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 7 cut(s) 66, 75, 94, 123, 260, 518, 826
BspANI GGCC 2 cut(s) 185, 638
BspCNI CTCAG 6 cut(s) 250, 262, 402, 484, 554, 625
BspLI GGNNCC 2 cut(s) 130, 429
BspMAI CTGCAG 1 cut(s) 721
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 48, 673
BsrFI RCCGGY 2 cut(s) 125, 131
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 2 cut(s) 125, 131
BssECI CCNNGG 2 cut(s) 61, 835
BssMI GATC 3 cut(s) 44, 402, 862
BssNI GRCGYC 2 cut(s) 129, 192
BssSI CACGAG 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 104, 593
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 2 cut(s) 129, 192
BstC8I GCNNGC 5 cut(s) 127, 133, 183, 313, 966
BstDEI CTNAG 8 cut(s) 237, 270, 282, 389, 492, 541, 633, 738
BstDSI CCRYGG 1 cut(s) 61
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 5 cut(s) 33, 451, 556, 779, 891
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 3 cut(s) 71, 131, 573
BstKTI GATC 3 cut(s) 47, 405, 865
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 44, 402, 862
BstMWI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
BstNI CCWGG 2 cut(s) 104, 593
BstSCI CCNGG 2 cut(s) 102, 591
BstSFI CTRYAG 3 cut(s) 115, 717, 969
BstV1I GCAGC 3 cut(s) 703, 731, 806
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 2 cut(s) 185, 638
BtgI CCRYGG 1 cut(s) 61
BtrI CACGTC 2 cut(s) 81, 228
BtsCI GGATG 5 cut(s) 33, 451, 556, 779, 891
BtsIMutI CAGTG 1 cut(s) 705
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 5 cut(s) 127, 133, 183, 313, 966
CfoI GCGC 3 cut(s) 71, 131, 573
Cfr10I RCCGGY 2 cut(s) 125, 131
Cfr13I GGNCC 1 cut(s) 637
CseI GACGC 1 cut(s) 181
CsiI ACCWGGT 1 cut(s) 591
Csp6I GTAC 5 cut(s) 298, 304, 515, 727, 949
CviAII CATG 2 cut(s) 140, 804
CviQI GTAC 5 cut(s) 298, 304, 515, 727, 949
DdeI CTNAG 8 cut(s) 237, 270, 282, 389, 492, 541, 633, 738
DinI GGCGCC 1 cut(s) 130
DpnI GATC 3 cut(s) 46, 404, 864
DpnII GATC 3 cut(s) 44, 402, 862
DraIII CACNNNGTG 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 767
Eco81I CCTNAGG 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 2 cut(s) 102, 591
EcoT14I CCWWGG 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 276
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 835
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 2 cut(s) 143, 807
FatI CATG 2 cut(s) 139, 803
FauI CCCGC 1 cut(s) 819
FauNDI CATATG 1 cut(s) 600
Fnu4HI GCNGC 5 cut(s) 67, 260, 717, 720, 795
FokI GGATG 5 cut(s) 20, 458, 563, 766, 878
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 5 cut(s) 67, 260, 717, 720, 795
FspBI CTAG 2 cut(s) 458, 818
GlaI GCGC 3 cut(s) 70, 130, 572
GluI GCNGC 5 cut(s) 67, 260, 717, 720, 795
GsuI CTGGAG 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 2 cut(s) 185, 638
HapII CCGG 4 cut(s) 126, 132, 198, 266
HgaI GACGC 1 cut(s) 181
HhaI GCGC 3 cut(s) 71, 131, 573
Hin1I GRCGYC 2 cut(s) 129, 192
Hin1II CATG 2 cut(s) 143, 807
Hin6I GCGC 3 cut(s) 69, 129, 571
HinP1I GCGC 3 cut(s) 69, 129, 571
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 4 cut(s) 161, 464, 499, 644
HpaII CCGG 4 cut(s) 126, 132, 198, 266
HphI GGTGA 5 cut(s) 75, 391, 581, 600, 953
Hpy166II GTNNAC 2 cut(s) 247, 382
Hpy188I TCNGA 5 cut(s) 415, 432, 472, 544, 634
Hpy188III TCNNGA 5 cut(s) 42, 239, 506, 818, 954
Hpy8I GTNNAC 2 cut(s) 247, 382
Hpy99I CGWCG 2 cut(s) 191, 197
HpyAV CCTTC 4 cut(s) 37, 107, 418, 990
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 3 cut(s) 80, 227, 775
HpyCH4V TGCA 4 cut(s) 324, 598, 719, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
HpyF3I CTNAG 8 cut(s) 237, 270, 282, 389, 492, 541, 633, 738
HpySE526I ACGT 3 cut(s) 80, 227, 775
Hsp92I GRCGYC 2 cut(s) 129, 192
Hsp92II CATG 2 cut(s) 143, 807
HspAI GCGC 3 cut(s) 69, 129, 571
KasI GGCGCC 1 cut(s) 128
KroI GCCGGC 2 cut(s) 125, 131
KroNI GCCGGC 2 cut(s) 127, 133
Kzo9I GATC 3 cut(s) 44, 402, 862
LmnI GCTCC 2 cut(s) 125, 961
Lsp1109I GCAGC 3 cut(s) 703, 731, 806
LweI GCATC 2 cut(s) 788, 802
MabI ACCWGGT 1 cut(s) 591
MaeI CTAG 2 cut(s) 458, 818
MaeII ACGT 3 cut(s) 80, 227, 775
MaeIII GTNAC 3 cut(s) 81, 228, 680
MalI GATC 3 cut(s) 46, 404, 864
MboI GATC 3 cut(s) 44, 402, 862
MboII GAAGA 5 cut(s) 494, 508, 569, 760, 763
MhlI GDGCHC 2 cut(s) 276, 930
MluCI AATT 6 cut(s) 369, 477, 673, 693, 830, 853
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 2 cut(s) 170, 653
MreI CGCCGGCG 1 cut(s) 125
MroNI GCCGGC 2 cut(s) 125, 131
MroXI GAANNNNTTC 2 cut(s) 10, 338
MseI TTAA 2 cut(s) 884, 991
MspI CCGG 4 cut(s) 126, 132, 198, 266
MspR9I CCNGG 2 cut(s) 104, 593
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 2 cut(s) 104, 593
MwoI GCNNNNNNNGC 5 cut(s) 126, 215, 321, 716, 866
NaeI GCCGGC 2 cut(s) 127, 133
NarI GGCGCC 1 cut(s) 129
NdeI CATATG 1 cut(s) 600
NdeII GATC 3 cut(s) 44, 402, 862
NgoMIV GCCGGC 2 cut(s) 125, 131
NlaIII CATG 2 cut(s) 143, 807
NlaIV GGNNCC 2 cut(s) 130, 429
NmuCI GTSAC 2 cut(s) 81, 228
PaqCI CACCTGC 1 cut(s) 564
PctI GAATGC 1 cut(s) 338
PdiI GCCGGC 2 cut(s) 127, 133
PdmI GAANNNNTTC 2 cut(s) 10, 338
PfeI GAWTC 2 cut(s) 464, 499
PkrI GCNGC 5 cut(s) 68, 261, 718, 721, 796
PleI GAGTC 2 cut(s) 169, 652
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 2 cut(s) 169, 652
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 102, 591
PspGI CCWGG 2 cut(s) 102, 591
PspN4I GGNNCC 2 cut(s) 130, 429
PspPI GGNCC 1 cut(s) 637
PstI CTGCAG 1 cut(s) 721
RsaI GTAC 5 cut(s) 299, 305, 516, 728, 950
RsaNI GTAC 5 cut(s) 298, 304, 515, 727, 949
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 884, 991
SatI GCNGC 5 cut(s) 67, 260, 717, 720, 795
Sau3AI GATC 3 cut(s) 44, 402, 862
Sau96I GGNCC 1 cut(s) 637
SchI GAGTC 2 cut(s) 170, 653
ScrFI CCNGG 2 cut(s) 104, 593
SduI GDGCHC 2 cut(s) 276, 930
SexAI ACCWGGT 1 cut(s) 591
SfaNI GCATC 2 cut(s) 788, 802
SfcI CTRYAG 3 cut(s) 115, 717, 969
SfoI GGCGCC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 641, 952
SmoI CTYRAG 2 cut(s) 641, 952
Sse9I AATT 6 cut(s) 369, 477, 673, 693, 830, 853
SsiI CCGC 7 cut(s) 66, 75, 94, 123, 260, 518, 826
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 2 cut(s) 458, 818
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 102, 591
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 3 cut(s) 83, 230, 778
TaqI TCGA 1 cut(s) 159
TasI AATT 6 cut(s) 369, 477, 673, 693, 830, 853
TatI WGTACW 1 cut(s) 726
TauI GCSGC 2 cut(s) 69, 262
TfiI GAWTC 2 cut(s) 464, 499
Tru1I TTAA 2 cut(s) 884, 991
Tru9I TTAA 2 cut(s) 884, 991
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 2 cut(s) 81, 228
TseI GCWGC 3 cut(s) 716, 719, 794
Tsp45I GTSAC 2 cut(s) 81, 228
TspDTI ATGAA 4 cut(s) 456, 570, 786, 804
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 1 cut(s) 705
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 830
XbaI TCTAGA 1 cut(s) 817
XmnI GAANNNNTTC 2 cut(s) 10, 338
XspI CTAG 2 cut(s) 458, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.