MD08G1010100.v1.1

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003258
Physical Location & Seq
Reverse (-)
752110 .. 754561
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1010100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGAGAAGGATTAGCGGAATCGCTTTGCTCACCAGAGCCGCCGTTTCTATGGGCGGCGCCACCACAGCCACCTCCGCAACTCAGCACCGTCTGGTTCAAGCAGCGATGTTGAGCACGTGCTCGAATTCCAATCCGCTGTGGTTCACACAGAGACTCGGCGATCGCTTCGGCCTATCGAGACCCTCCACTGCGGCTCCCTGTGCCGGAGGGCACGTGTGCTTCTCCACAAACGCCGCCGCTTATCTGGCTCAGGAGCCGCACGCCCATGAAAAGTTCCTCTCCAAAGACGTCGAGCTCTACCAGTACGAAGCTTGCCCTTTCTGCAACAAGGTTAGAGCATTCCTGGATTACTATAACATTCCGTACAAAGTTGTGGAGGTTAACCCCATTAGCAAAAAGGAGATTAATTGGTCCGATTACAAGAAGGTGCCAATCCTGAAAGTCGACGGCGAACAGATGGTTGACTCTTCAGATATAATTGATAAGCTGTACCTAAGGATCAACGAGAACCCCGTTTTAAATGGTGAAGAAGAAATGAAGTGGCGCGGGTGGGTGGATAATCACTTGGTGCATGTTTTATCACCAAACATATATAGAACTGTTTCAGAGGCTCTCGAGTCGTTTGACTACATCACCAGTCATGGAAACTTTAGTCTGTATGAAAGGATGGTAGCGAAGTATACCGGAGCTGCTGCTATGTATTTTGTGTCAAAGAAACTGAAGAAGAGGCACAATATTACTGATGAACGTGCATCCTTGTATGGAGCTGCAGAAACATGGGTTGATGCTCTCAATGGCAGACAATTTCTTGGTGGATCAAATCCTAATTTAGCCGATCTTGCTGTATTTGGTGTTTTGAGGCCCATTCGACACCTTAAATCCGGCAAAGATATGGTGGAGAACACGCGGATTGGTGAATGGTATGCTCGCATGGAAAATGCAGTGGGAGAGTCTGCTAGAGTCAAGGCCTAA

Protein Analysis

324

Amino Acids

36.23

Weight (kDa)

8.81

Isoelectric Point (pI)

25.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 97 - 151 1.1e-09 Glutaredoxin
GST_N_3 PF13417 99 - 166 1.3e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 104 - 163 1.3e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 106 - 189 3e-07 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
AccB1I GGYRCC 2 cut(s) 56, 427
AccI GTMKAC 2 cut(s) 444, 680
AccII CGCG 2 cut(s) 546, 907
AclWI GGATC 2 cut(s) 506, 823
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 2 cut(s) 453, 740
AcvI CACGTG 2 cut(s) 117, 214
AcyI GRCGYC 2 cut(s) 57, 288
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 3 cut(s) 305, 365, 491
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 1 cut(s) 98
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 5 cut(s) 295, 311, 487, 689, 767
AluI AGCT 5 cut(s) 295, 311, 487, 689, 767
Alw21I GWGCWC 3 cut(s) 116, 122, 297
Alw26I GTCTC 2 cut(s) 145, 172
AlwI GGATC 2 cut(s) 506, 823
Ama87I CYCGRG 1 cut(s) 614
AoxI GGCC 3 cut(s) 169, 860, 966
ApeKI GCWGC 4 cut(s) 101, 689, 692, 767
ApoI RAATTY 1 cut(s) 124
AseI ATTAAT 1 cut(s) 405
AsiSI GCGATCGC 1 cut(s) 163
Asp700I GAANNNNTTC 1 cut(s) 601
AspLEI GCGC 2 cut(s) 59, 546
AspS9I GGNCC 2 cut(s) 411, 861
AsuHPI GGTGA 5 cut(s) 22, 536, 573, 625, 926
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 1 cut(s) 411
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 2 cut(s) 56, 427
BanII GRGCYC 1 cut(s) 297
BbrPI CACGTG 2 cut(s) 117, 214
Bbv12I GWGCWC 3 cut(s) 116, 122, 297
BbvI GCAGC 4 cut(s) 113, 676, 679, 754
BccI CCATC 2 cut(s) 451, 661
BceAI ACGGC 2 cut(s) 26, 463
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 145, 172
BfaI CTAG 1 cut(s) 957
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 2 cut(s) 411, 861
BmiI GGNNCC 4 cut(s) 58, 195, 255, 429
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 2 cut(s) 761, 775
Bpu10I CCTNAGC 1 cut(s) 249
BsaAI YACGTR 2 cut(s) 117, 214
BsaHI GRCGYC 2 cut(s) 57, 288
BsaI GGTCTC 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 4 cut(s) 178, 198, 208, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 301, 636
Bse21I CCTNAGG 1 cut(s) 494
BseBI CCWGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 672, 752
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 2 cut(s) 95, 263
BseNI ACTGG 2 cut(s) 301, 636
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 4 cut(s) 113, 676, 679, 754
Bsh1236I CGCG 2 cut(s) 546, 907
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 3 cut(s) 171, 862, 968
BshNI GGYRCC 2 cut(s) 56, 427
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 3 cut(s) 116, 122, 297
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 3 cut(s) 204, 684, 882
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 145, 172
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 3 cut(s) 171, 862, 968
Bso31I GGTCTC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 614
Bsp1286I GDGCHC 4 cut(s) 116, 122, 213, 297
Bsp143I GATC 4 cut(s) 160, 498, 815, 835
BspANI GGCC 3 cut(s) 171, 862, 968
BspCNI CTCAG 2 cut(s) 94, 262
BspFNI CGCG 2 cut(s) 546, 907
BspLI GGNNCC 4 cut(s) 58, 195, 255, 429
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 2 cut(s) 506, 823
BspT107I GGYRCC 2 cut(s) 56, 427
BspTNI GGTCTC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 301, 636
BssMI GATC 4 cut(s) 160, 498, 815, 835
BssNAI GTATAC 1 cut(s) 681
BssNI GRCGYC 2 cut(s) 57, 288
Bst1107I GTATAC 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 89, 601
Bst6I CTCTTC 2 cut(s) 472, 719
BstACI GRCGYC 2 cut(s) 57, 288
BstBAI YACGTR 2 cut(s) 117, 214
BstC8I GCNNGC 3 cut(s) 261, 313, 928
BstDEI CTNAG 3 cut(s) 81, 249, 494
BstF5I GGATG 2 cut(s) 672, 752
BstFNI CGCG 2 cut(s) 546, 907
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 546
BstKTI GATC 4 cut(s) 163, 501, 818, 838
BstMAI GTCTC 2 cut(s) 145, 172
BstMBI GATC 4 cut(s) 160, 498, 815, 835
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 839
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 2 cut(s) 546, 907
BstV1I GCAGC 4 cut(s) 113, 676, 679, 754
BstZ17I GTATAC 1 cut(s) 681
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 3 cut(s) 171, 862, 968
BtgZI GCGATG 1 cut(s) 119
BtsCI GGATG 2 cut(s) 672, 752
BtsI GCAGTG 2 cut(s) 186, 948
BtsIMutI CAGTG 2 cut(s) 186, 948
Cac8I GCNNGC 3 cut(s) 261, 313, 928
CfoI GCGC 2 cut(s) 59, 546
Cfr13I GGNCC 2 cut(s) 411, 861
Csp6I GTAC 3 cut(s) 304, 364, 490
CviAII CATG 5 cut(s) 266, 572, 641, 777, 931
CviQI GTAC 3 cut(s) 304, 364, 490
DdeI CTNAG 3 cut(s) 81, 249, 494
DinI GGCGCC 1 cut(s) 58
DpnI GATC 4 cut(s) 162, 500, 817, 837
DpnII GATC 4 cut(s) 160, 498, 815, 835
DraI TTTAAA 1 cut(s) 519
DraIII CACNNNGTG 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 472, 719
EarI CTCTTC 2 cut(s) 472, 719
Ecl136II GAGCTC 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 453, 740
Eco72I CACGTG 2 cut(s) 117, 214
Eco81I CCTNAGG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 5 cut(s) 269, 575, 644, 780, 934
FatI CATG 5 cut(s) 265, 571, 640, 776, 930
FauI CCCGC 1 cut(s) 539
FblI GTMKAC 2 cut(s) 444, 680
FokI GGATG 2 cut(s) 679, 739
FriOI GRGCYC 1 cut(s) 297
FspBI CTAG 1 cut(s) 957
GlaI GCGC 2 cut(s) 58, 545
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 171, 862, 968
HapII CCGG 3 cut(s) 204, 684, 882
HhaI GCGC 2 cut(s) 59, 546
Hin1I GRCGYC 2 cut(s) 57, 288
Hin1II CATG 5 cut(s) 269, 575, 644, 780, 934
Hin6I GCGC 2 cut(s) 57, 544
HinP1I GCGC 2 cut(s) 57, 544
HincII GTYRAC 3 cut(s) 382, 445, 463
HindII GTYRAC 3 cut(s) 382, 445, 463
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 6 cut(s) 18, 153, 464, 617, 950, 960
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 3 cut(s) 204, 684, 882
HphI GGTGA 5 cut(s) 22, 536, 573, 625, 926
Hpy166II GTNNAC 5 cut(s) 144, 382, 445, 463, 681
Hpy188I TCNGA 3 cut(s) 415, 472, 607
Hpy188III TCNNGA 4 cut(s) 177, 251, 436, 614
Hpy8I GTNNAC 5 cut(s) 144, 382, 445, 463, 681
Hpy99I CGWCG 2 cut(s) 293, 449
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 89, 601
HpyCH4IV ACGT 4 cut(s) 116, 213, 288, 748
HpyCH4V TGCA 5 cut(s) 324, 571, 752, 770, 941
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 839
HpyF3I CTNAG 3 cut(s) 81, 249, 494
HpySE526I ACGT 4 cut(s) 116, 213, 288, 748
Hsp92I GRCGYC 2 cut(s) 57, 288
Hsp92II CATG 5 cut(s) 269, 575, 644, 780, 934
HspAI GCGC 2 cut(s) 57, 544
KasI GGCGCC 1 cut(s) 56
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 160, 498, 815, 835
LmnI GCTCC 4 cut(s) 199, 253, 686, 764
Lsp1109I GCAGC 4 cut(s) 113, 676, 679, 754
LweI GCATC 2 cut(s) 761, 775
MaeI CTAG 1 cut(s) 957
MaeII ACGT 4 cut(s) 116, 213, 288, 748
MalI GATC 4 cut(s) 162, 500, 817, 837
MboI GATC 4 cut(s) 160, 498, 815, 835
MboII GAAGA 5 cut(s) 459, 539, 542, 733, 736
MhlI GDGCHC 4 cut(s) 116, 122, 213, 297
MluCI AATT 5 cut(s) 124, 406, 477, 803, 826
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 5 cut(s) 147, 458, 626, 959, 969
MnlI CCTC 8 cut(s) 82, 193, 200, 287, 370, 601, 720, 852
MroXI GAANNNNTTC 1 cut(s) 601
MseI TTAA 4 cut(s) 381, 405, 518, 876
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 3 cut(s) 204, 684, 882
MspR9I CCNGG 1 cut(s) 344
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 2 cut(s) 546, 907
MwoI GCNNNNNNNGC 6 cut(s) 65, 74, 200, 245, 321, 839
NarI GGCGCC 1 cut(s) 57
NdeII GATC 4 cut(s) 160, 498, 815, 835
NlaIII CATG 5 cut(s) 269, 575, 644, 780, 934
NlaIV GGNNCC 4 cut(s) 58, 195, 255, 429
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 1 cut(s) 575
PaeR7I CTCGAG 1 cut(s) 614
PceI AGGCCT 1 cut(s) 968
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 1 cut(s) 338
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 342
Ple19I CGATCG 1 cut(s) 163
PleI GAGTC 5 cut(s) 147, 458, 625, 958, 968
PluTI GGCGCC 1 cut(s) 60
PmaCI CACGTG 2 cut(s) 117, 214
PmlI CACGTG 2 cut(s) 117, 214
PpsI GAGTC 5 cut(s) 147, 458, 625, 958, 968
Ppu21I YACGTR 2 cut(s) 117, 214
PshBI ATTAAT 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 342
PspCI CACGTG 2 cut(s) 117, 214
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 4 cut(s) 58, 195, 255, 429
PspPI GGNCC 2 cut(s) 411, 861
PstI CTGCAG 1 cut(s) 772
PvuI CGATCG 1 cut(s) 163
RgaI GCGATCGC 1 cut(s) 163
RsaI GTAC 3 cut(s) 305, 365, 491
RsaNI GTAC 3 cut(s) 304, 364, 490
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 297
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 4 cut(s) 381, 405, 518, 876
Sau3AI GATC 4 cut(s) 160, 498, 815, 835
Sau96I GGNCC 2 cut(s) 411, 861
SchI GAGTC 5 cut(s) 147, 458, 626, 959, 969
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 4 cut(s) 116, 122, 213, 297
SfaAI GCGATCGC 1 cut(s) 163
SfaNI GCATC 2 cut(s) 761, 775
SfcI CTRYAG 1 cut(s) 768
SfoI GGCGCC 1 cut(s) 58
Sfr274I CTCGAG 1 cut(s) 614
SgfI GCGATCGC 1 cut(s) 163
SinI GGWCC 1 cut(s) 411
SlaI CTCGAG 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 5 cut(s) 124, 406, 477, 803, 826
SseBI AGGCCT 1 cut(s) 968
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 1 cut(s) 736
SspMI CTAG 1 cut(s) 957
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 89, 601
TaiI ACGT 4 cut(s) 119, 216, 291, 751
TaqI TCGA 6 cut(s) 122, 176, 291, 444, 615, 868
TasI AATT 5 cut(s) 124, 406, 477, 803, 826
TauI GCSGC 6 cut(s) 41, 57, 194, 236, 239, 259
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 4 cut(s) 381, 405, 518, 876
Tru9I TTAA 4 cut(s) 381, 405, 518, 876
TscAI CASTG 2 cut(s) 193, 948
TseI GCWGC 4 cut(s) 101, 689, 692, 767
TspDTI ATGAA 4 cut(s) 282, 551, 675, 759
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 2 cut(s) 193, 948
VpaK11BI GGWCC 1 cut(s) 411
VspI ATTAAT 1 cut(s) 405
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 614
XmiI GTMKAC 2 cut(s) 444, 680
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 1 cut(s) 957
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.