Rh7DG014800

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003258
Physical Location & Seq
Forward (+)
1206707 .. 1208820
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG014800.1

Sequence Viewer

Length: 912 bp
ATGAGAAGGGCTTCCACTCTCGCCTCCTTCCCCATCATATCCCGATCCATCGCCACCCTCCACGGCGGCGCAACCGCCACGTCACCCTCCCACCGCCTTCTCCAGGTAGCTCTCTACAGCTCCGCCGGCTCTCATGGTCGCCGTCCCTGGTTCGAGTCATTTTCCGGCCGGAAGCTGGCCGTCGGCGTCGCCGGAACTATGGCTTCGGTGGCTGTTGCCACGTCGCTCGCTCAGGAAGTCCACGCCAAAGAGCCGCCTCCGGCTGAGCTCGTGCCTAAGGAGGTTGTGCTGTACCAGTACGAAGCTTGCCCTTTCTGCAACAAAGTGAAAGCATTCTTGGACTACTATGATATACCGTACAAAATTGTGGAGGTGAATCCACTCAGTAAGAAAGAGATCAAGTTTTCAGAGTATAAGAAGGTTCCGATATTGATGGTGGATGGGGAACAGCTAGTTGATTCATCAGATATAATTGATGCCTTGACTGAGAAGATTCTTCCTGACCGAGTACCGCAGAGAGTAGCAGTTTCTACCTCAGATGAGGATGATGAAGAGAAAAAGTGGCGCAAGTGGGTTGATAATCACCTGGTGCATATGCTATCACCAAACATATACCGAAATACATCTGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTTAGTTACACAGAAAAAATTACAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTGTCTAAGAAACTGAAGAAGAAATATAACATTACTGATGAACGTGCATCCCTGTATGAAGCAGCAGAAACATGGGTTGATGCTTTAGATGGGCGGGAATTCCTTGGTATGTGCTCTAACTTGTGGCCTATGCACGTTGTGTTCCATAGAGATCGCATGAGATTATTACTTTTAATTAGTTGGTGA

Protein Analysis

303

Amino Acids

34.25

Weight (kDa)

7.71

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 95 - 149 3.3e-10 Glutaredoxin
GST_N_3 PF13417 97 - 163 2e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 102 - 163 2.8e-09 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 104 - 200 3.3e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 66, 75, 94, 123, 254, 512, 820
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 2 cut(s) 166, 177
AcsI RAATTY 1 cut(s) 824
AcuI CTGAAG 1 cut(s) 761
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 2 cut(s) 589, 865
AfaI GTAC 5 cut(s) 293, 299, 359, 510, 722
AfiI CCNNNNNNNGG 2 cut(s) 103, 175
AjiI CACGTC 2 cut(s) 81, 222
AjnI CCWGG 3 cut(s) 102, 146, 585
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 7 cut(s) 110, 120, 175, 268, 305, 451, 716
AluI AGCT 7 cut(s) 110, 120, 175, 268, 305, 451, 716
Alw21I GWGCWC 2 cut(s) 270, 842
AlwI GGATC 1 cut(s) 39
AoxI GGCC 4 cut(s) 166, 177, 630, 851
ApeKI GCWGC 3 cut(s) 710, 713, 788
ApoI RAATTY 1 cut(s) 824
Asp700I GAANNNNTTC 2 cut(s) 10, 332
AspLEI GCGC 2 cut(s) 71, 567
AspS9I GGNCC 1 cut(s) 631
AsuHPI GGTGA 4 cut(s) 75, 385, 575, 594
AxyI CCTNAGG 1 cut(s) 276
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 270, 842
BbvI GCAGC 3 cut(s) 697, 725, 800
BccI CCATC 5 cut(s) 41, 56, 427, 434, 809
BceAI ACGGC 3 cut(s) 79, 126, 164
BciT130I CCWGG 3 cut(s) 104, 148, 587
BfaI CTAG 1 cut(s) 452
BfmI CTRYAG 2 cut(s) 115, 711
BisI GCNGC 5 cut(s) 67, 254, 711, 714, 789
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 5 cut(s) 68, 255, 712, 715, 790
Bme1390I CCNGG 3 cut(s) 104, 148, 587
BmgBI CACGTC 2 cut(s) 81, 222
BmgT120I GGNCC 1 cut(s) 631
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 3 cut(s) 104, 148, 587
BmsI GCATC 3 cut(s) 466, 782, 796
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 231
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 61, 146, 829
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 175
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 295
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 667
BseBI CCWGG 3 cut(s) 104, 148, 587
BseDI CCNNGG 3 cut(s) 61, 146, 829
BseGI GGATG 3 cut(s) 445, 550, 773
BseLI CCNNNNNNNGG 2 cut(s) 103, 175
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 6 cut(s) 245, 255, 397, 477, 549, 618
BseNI ACTGG 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 697, 725, 800
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 4 cut(s) 168, 179, 632, 853
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 270, 842
BsiSI CCGG 5 cut(s) 126, 165, 169, 192, 260
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 103, 175
BsmFI GGGAC 1 cut(s) 129
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 4 cut(s) 168, 179, 632, 853
Bsp1286I GDGCHC 2 cut(s) 270, 842
Bsp143I GATC 3 cut(s) 44, 396, 877
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 7 cut(s) 66, 75, 94, 123, 254, 512, 820
BspANI GGCC 4 cut(s) 168, 179, 632, 853
BspCNI CTCAG 6 cut(s) 244, 256, 396, 478, 548, 619
BspLI GGNNCC 1 cut(s) 423
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 3 cut(s) 61, 146, 829
BssMI GATC 3 cut(s) 44, 396, 877
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 829
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 3 cut(s) 104, 148, 587
Bst4CI ACNGT 2 cut(s) 357, 694
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 4 cut(s) 127, 177, 228, 307
BstDEI CTNAG 8 cut(s) 231, 264, 276, 383, 486, 535, 627, 732
BstDSI CCRYGG 1 cut(s) 61
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 3 cut(s) 445, 550, 773
BstHHI GCGC 2 cut(s) 71, 567
BstKTI GATC 3 cut(s) 47, 399, 880
BstMBI GATC 3 cut(s) 44, 396, 877
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 4 cut(s) 126, 209, 315, 710
BstNI CCWGG 3 cut(s) 104, 148, 587
BstSCI CCNGG 3 cut(s) 102, 146, 585
BstSFI CTRYAG 2 cut(s) 115, 711
BstV1I GCAGC 3 cut(s) 697, 725, 800
BstZI CGGCCG 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 276
BsuRI GGCC 4 cut(s) 168, 179, 632, 853
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 34
BtrI CACGTC 2 cut(s) 81, 222
BtsCI GGATG 3 cut(s) 445, 550, 773
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 4 cut(s) 127, 177, 228, 307
CfoI GCGC 2 cut(s) 71, 567
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 631
CseI GACGC 1 cut(s) 175
CsiI ACCWGGT 1 cut(s) 585
Csp6I GTAC 5 cut(s) 292, 298, 358, 509, 721
CviAII CATG 3 cut(s) 134, 798, 883
CviQI GTAC 5 cut(s) 292, 298, 358, 509, 721
DdeI CTNAG 8 cut(s) 231, 264, 276, 383, 486, 535, 627, 732
DpnI GATC 3 cut(s) 46, 398, 879
DpnII GATC 3 cut(s) 44, 396, 877
DraIII CACNNNGTG 2 cut(s) 589, 865
EaeI YGGCCR 2 cut(s) 166, 177
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 829
Eco24I GRGCYC 1 cut(s) 270
Eco52I CGGCCG 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 761
Eco81I CCTNAGG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 631
EcoRI GAATTC 1 cut(s) 824
EcoRII CCWGG 3 cut(s) 102, 146, 585
EcoT14I CCWWGG 1 cut(s) 829
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 829
FaeI CATG 3 cut(s) 137, 801, 886
FaqI GGGAC 1 cut(s) 129
FatI CATG 3 cut(s) 133, 797, 882
FauI CCCGC 1 cut(s) 813
FauNDI CATATG 1 cut(s) 594
Fnu4HI GCNGC 5 cut(s) 67, 254, 711, 714, 789
FokI GGATG 3 cut(s) 452, 557, 760
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 5 cut(s) 67, 254, 711, 714, 789
FspBI CTAG 1 cut(s) 452
GlaI GCGC 2 cut(s) 70, 566
GluI GCNGC 5 cut(s) 67, 254, 711, 714, 789
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 4 cut(s) 168, 179, 632, 853
HapII CCGG 5 cut(s) 126, 165, 169, 192, 260
HgaI GACGC 1 cut(s) 175
HhaI GCGC 2 cut(s) 71, 567
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 137, 801, 886
Hin6I GCGC 2 cut(s) 69, 565
HinP1I GCGC 2 cut(s) 69, 565
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 5 cut(s) 155, 376, 458, 493, 638
HpaII CCGG 5 cut(s) 126, 165, 169, 192, 260
HphI GGTGA 4 cut(s) 75, 385, 575, 594
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 5 cut(s) 409, 426, 466, 538, 628
Hpy188III TCNNGA 3 cut(s) 42, 233, 500
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 3 cut(s) 185, 191, 226
HpyAV CCTTC 3 cut(s) 37, 107, 412
HpyCH4III ACNGT 2 cut(s) 357, 694
HpyCH4IV ACGT 4 cut(s) 80, 221, 769, 861
HpyCH4V TGCA 5 cut(s) 318, 592, 713, 773, 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 209, 315, 710
HpyF3I CTNAG 8 cut(s) 231, 264, 276, 383, 486, 535, 627, 732
HpySE526I ACGT 4 cut(s) 80, 221, 769, 861
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 137, 801, 886
HspAI GCGC 2 cut(s) 69, 565
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 44, 396, 877
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 3 cut(s) 697, 725, 800
LweI GCATC 3 cut(s) 466, 782, 796
MabI ACCWGGT 1 cut(s) 585
MaeI CTAG 1 cut(s) 452
MaeII ACGT 4 cut(s) 80, 221, 769, 861
MaeIII GTNAC 2 cut(s) 81, 674
MalI GATC 3 cut(s) 46, 398, 879
MboI GATC 3 cut(s) 44, 396, 877
MboII GAAGA 5 cut(s) 488, 502, 563, 754, 757
MhlI GDGCHC 2 cut(s) 270, 842
MluCI AATT 6 cut(s) 363, 471, 667, 687, 824, 900
MlyI GAGTC 2 cut(s) 164, 647
MnlI CCTC 9 cut(s) 34, 68, 97, 267, 274, 364, 535, 544, 622
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 2 cut(s) 10, 332
MseI TTAA 1 cut(s) 899
MspI CCGG 5 cut(s) 126, 165, 169, 192, 260
MspR9I CCNGG 3 cut(s) 104, 148, 587
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 3 cut(s) 104, 148, 587
MwoI GCNNNNNNNGC 4 cut(s) 126, 209, 315, 710
NaeI GCCGGC 1 cut(s) 127
NdeI CATATG 1 cut(s) 594
NdeII GATC 3 cut(s) 44, 396, 877
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 3 cut(s) 137, 801, 886
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 81
PctI GAATGC 1 cut(s) 332
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 10, 332
PfeI GAWTC 3 cut(s) 376, 458, 493
PkrI GCNGC 5 cut(s) 68, 255, 712, 715, 790
PleI GAGTC 2 cut(s) 163, 646
PpsI GAGTC 2 cut(s) 163, 646
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 3 cut(s) 102, 146, 585
PspGI CCWGG 3 cut(s) 102, 146, 585
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 631
PstI CTGCAG 1 cut(s) 715
RsaI GTAC 5 cut(s) 293, 299, 359, 510, 722
RsaNI GTAC 5 cut(s) 292, 298, 358, 509, 721
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 899
SatI GCNGC 5 cut(s) 67, 254, 711, 714, 789
Sau3AI GATC 3 cut(s) 44, 396, 877
Sau96I GGNCC 1 cut(s) 631
SchI GAGTC 2 cut(s) 164, 647
ScrFI CCNGG 3 cut(s) 104, 148, 587
SduI GDGCHC 2 cut(s) 270, 842
SexAI ACCWGGT 1 cut(s) 585
SfaNI GCATC 3 cut(s) 466, 782, 796
SfcI CTRYAG 2 cut(s) 115, 711
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 6 cut(s) 363, 471, 667, 687, 824, 900
SsiI CCGC 7 cut(s) 66, 75, 94, 123, 254, 512, 820
SspMI CTAG 1 cut(s) 452
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 102, 146, 585
StyI CCWWGG 1 cut(s) 829
TaaI ACNGT 2 cut(s) 357, 694
TaiI ACGT 4 cut(s) 83, 224, 772, 864
TaqI TCGA 1 cut(s) 153
TaqII GACCGA 1 cut(s) 519
TasI AATT 6 cut(s) 363, 471, 667, 687, 824, 900
TatI WGTACW 1 cut(s) 720
TauI GCSGC 2 cut(s) 69, 256
TfiI GAWTC 3 cut(s) 376, 458, 493
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 3 cut(s) 710, 713, 788
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 4 cut(s) 450, 564, 780, 798
TspRI CASTG 1 cut(s) 699
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 824
XmnI GAANNNNTTC 2 cut(s) 10, 332
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.