Rh6DG433800

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003258
Physical Location & Seq
Forward (+)
61245885 .. 61248974
3090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG433800.1

Sequence Viewer

Length: 825 bp
ATGAGAAGGGTTAACGGAATCGCTTTGCTCGGCCGCACCGCCGCTGGTATTGGCGGCGCCACCAGAGTCAGCGGCGCAACTCCACAGCGACTGGTTCAAGCAGCGACGATAGGCTCGTGCTCGAATTCGAATCCGCTGTGGTTCAGTCGTCAAAGCTCCGGCGATCGCTTTGGCCTATCGAAACCCTCAGTGGCTGCCGGAGGCGCCAAGTGCTTCTCTACTTCCGTGGCTCAGGAGGTGCACTCCCACGTCTCCGCCGATAAGTTCGCTCCGAACGACGTCGTTCTGTACCAGTACGAAGCCTGCCCTTTCTGCAACAAGGTTAGAGCATTTTTGGACTATAATAACATTCCGTACAAAGTTATGGAGGTTAATCCCATGAGCAAAAAGGAGATCAAATGGTCTGATTACAAGAAGGTGCCGATATTGAAAGTTGACGATGAACAGATGGTGGATTCTTCAGATATAATTGATAAGCTATTCCAAAGAATCAATCCTGAGAACAGTGTTGTCGATAGTGAGGAAGAAAAGAAGTGGCGCCAGTGGGTGGATGATCACTTGGTGCATGTTTTATCACCAAACATATATCGAACTCCTTCAGAAGCTCTTGAGTCATTTGATTATATAACCAGTCACGGGAATTTCAGTTTATCCGAAAGGCTAGTAGCCAAGTATGGTGGAGCTGCAGCAATGTATTTTGTATCAAAAAAATTGAAGAAGAGACACAATATCACCGATGCTCGTGCAGCTTTATATGGAGCTGCAGAGACTTGGGTGGATGCTCTGAAAGGCCGGCAGTTTCTTGGTCAGCTCATCAGAATCTGA

Protein Analysis

274

Amino Acids

30.51

Weight (kDa)

9.14

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 94 - 148 1.4e-09 Glutaredoxin
GST_N_3 PF13417 96 - 165 7e-16 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 101 - 160 1.2e-08 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 103 - 191 3.5e-08 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 4 cut(s) 56, 203, 418, 537
AccB7I CCANNNNNTGG 1 cut(s) 547
AciI CCGC 7 cut(s) 34, 39, 42, 54, 72, 134, 255
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 2 cut(s) 124, 640
AcuI CTGAAG 2 cut(s) 444, 582
AcyI GRCGYC 4 cut(s) 57, 204, 279, 538
AdeI CACNNNGTG 1 cut(s) 562
AfaI GTAC 3 cut(s) 290, 296, 356
AfiI CCNNNNNNNGG 2 cut(s) 547, 636
AgsI TTSAA 3 cut(s) 98, 430, 715
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 7 cut(s) 156, 478, 605, 683, 749, 761, 811
AluI AGCT 7 cut(s) 156, 478, 605, 683, 749, 761, 811
Alw21I GWGCWC 2 cut(s) 122, 243
Alw26I GTCTC 3 cut(s) 256, 715, 761
Alw44I GTGCAC 1 cut(s) 239
AlwNI CAGNNNCTG 3 cut(s) 91, 194, 822
AoxI GGCC 3 cut(s) 31, 172, 790
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 6 cut(s) 101, 194, 683, 686, 746, 761
ApoI RAATTY 2 cut(s) 124, 640
AsiSI GCGATCGC 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 595
AspLEI GCGC 4 cut(s) 59, 77, 206, 540
AsuHPI GGTGA 2 cut(s) 567, 724
AsuII TTCGAA 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 243
BanI GGYRCC 4 cut(s) 56, 203, 418, 537
BauI CACGAG 2 cut(s) 115, 741
Bbv12I GWGCWC 2 cut(s) 122, 243
BbvI GCAGC 6 cut(s) 113, 181, 670, 698, 748, 758
BccI CCATC 1 cut(s) 442
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 3 cut(s) 256, 715, 761
BfaI CTAG 1 cut(s) 662
BfmI CTRYAG 2 cut(s) 684, 762
BfoI RGCGCY 3 cut(s) 60, 207, 541
BmgBI CACGTC 1 cut(s) 250
BmiI GGNNCC 4 cut(s) 58, 205, 420, 539
BmsI GCATC 2 cut(s) 727, 769
BplI GAGNNNNNCTC 2 cut(s) 227, 259
Bpu10I CCTNAGC 1 cut(s) 231
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 629
BsaHI GRCGYC 4 cut(s) 57, 204, 279, 538
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 636
Bse118I RCCGGY 1 cut(s) 792
Bse1I ACTGG 4 cut(s) 96, 292, 541, 630
Bse3DI GCAATG 1 cut(s) 696
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 556, 784
BseLI CCNNNNNNNGG 2 cut(s) 547, 636
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 3 cut(s) 201, 245, 489
BseNI ACTGG 4 cut(s) 96, 292, 541, 630
BseSI GKGCMC 1 cut(s) 243
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 6 cut(s) 113, 181, 670, 698, 748, 758
BsgI GTGCAG 1 cut(s) 765
Bsh1285I CGRYCG 2 cut(s) 34, 166
BshFI GGCC 3 cut(s) 33, 174, 792
BshNI GGYRCC 4 cut(s) 56, 203, 418, 537
BsiEI CGRYCG 2 cut(s) 34, 166
BsiHKAI GWGCWC 2 cut(s) 122, 243
BsiSI CCGG 3 cut(s) 159, 198, 793
BslI CCNNNNNNNGG 2 cut(s) 547, 636
BsmAI GTCTC 3 cut(s) 256, 715, 761
BsmBI CGTCTC 1 cut(s) 256
BsnI GGCC 3 cut(s) 33, 174, 792
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 122, 243
Bsp143I GATC 3 cut(s) 163, 393, 553
BspACI CCGC 7 cut(s) 34, 39, 42, 54, 72, 134, 255
BspANI GGCC 3 cut(s) 33, 174, 792
BspCNI CTCAG 3 cut(s) 200, 244, 490
BspLI GGNNCC 4 cut(s) 58, 205, 420, 539
BspMAI CTGCAG 2 cut(s) 688, 766
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 4 cut(s) 56, 203, 418, 537
BsrDI GCAATG 1 cut(s) 696
BsrFI RCCGGY 1 cut(s) 792
BsrI ACTGG 4 cut(s) 96, 292, 541, 630
BssAI RCCGGY 1 cut(s) 792
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 3 cut(s) 163, 393, 553
BssNI GRCGYC 4 cut(s) 57, 204, 279, 538
BssSI CACGAG 2 cut(s) 115, 741
Bst2BI CACGAG 2 cut(s) 115, 741
Bst4CI ACNGT 1 cut(s) 506
Bst6I CTCTTC 1 cut(s) 713
BstACI GRCGYC 4 cut(s) 57, 204, 279, 538
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 304, 794
BstDEI CTNAG 3 cut(s) 187, 231, 498
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 556, 784
BstH2I RGCGCY 3 cut(s) 60, 207, 541
BstHHI GCGC 4 cut(s) 59, 77, 206, 540
BstKTI GATC 3 cut(s) 166, 396, 556
BstMAI GTCTC 3 cut(s) 256, 715, 761
BstMBI GATC 3 cut(s) 163, 393, 553
BstMCI CGRYCG 2 cut(s) 34, 166
BstMWI GCNNNNNNNGC 4 cut(s) 203, 210, 312, 746
BstNSI RCATGY 1 cut(s) 569
BstSFI CTRYAG 2 cut(s) 684, 762
BstSLI GKGCMC 1 cut(s) 243
BstV1I GCAGC 6 cut(s) 113, 181, 670, 698, 748, 758
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 3 cut(s) 33, 174, 792
BtgI CCRYGG 1 cut(s) 225
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 2 cut(s) 556, 784
BtsIMutI CAGTG 3 cut(s) 195, 511, 548
Cac8I GCNNGC 2 cut(s) 304, 794
CaiI CAGNNNCTG 3 cut(s) 91, 194, 822
CfoI GCGC 4 cut(s) 59, 77, 206, 540
Cfr10I RCCGGY 1 cut(s) 792
Csp6I GTAC 3 cut(s) 289, 295, 355
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 2 cut(s) 379, 566
CviQI GTAC 3 cut(s) 289, 295, 355
DdeI CTNAG 3 cut(s) 187, 231, 498
DinI GGCGCC 3 cut(s) 58, 205, 539
DpnI GATC 3 cut(s) 165, 395, 555
DpnII GATC 3 cut(s) 163, 393, 553
DraIII CACNNNGTG 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
EciI GGCGGA 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 31
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 444, 582
EcoRI GAATTC 1 cut(s) 124
EgeI GGCGCC 3 cut(s) 58, 205, 539
EheI GGCGCC 3 cut(s) 58, 205, 539
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 2 cut(s) 382, 569
FatI CATG 2 cut(s) 378, 565
FbaI TGATCA 1 cut(s) 553
FokI GGATG 2 cut(s) 563, 791
FspBI CTAG 1 cut(s) 662
GlaI GCGC 4 cut(s) 58, 76, 205, 539
HaeII RGCGCY 3 cut(s) 60, 207, 541
HaeIII GGCC 3 cut(s) 33, 174, 792
HapII CCGG 3 cut(s) 159, 198, 793
HhaI GCGC 4 cut(s) 59, 77, 206, 540
Hin1I GRCGYC 4 cut(s) 57, 204, 279, 538
Hin1II CATG 2 cut(s) 382, 569
Hin6I GCGC 4 cut(s) 57, 75, 204, 538
HinP1I GCGC 4 cut(s) 57, 75, 204, 538
HincII GTYRAC 2 cut(s) 13, 436
HindII GTYRAC 2 cut(s) 13, 436
HinfI GANTC 7 cut(s) 18, 66, 130, 455, 489, 611, 819
HpaI GTTAAC 1 cut(s) 13
HpaII CCGG 3 cut(s) 159, 198, 793
HphI GGTGA 2 cut(s) 567, 724
Hpy166II GTNNAC 3 cut(s) 13, 241, 436
Hpy188I TCNGA 8 cut(s) 273, 406, 463, 601, 655, 786, 818, 824
Hpy188III TCNNGA 3 cut(s) 233, 497, 608
Hpy8I GTNNAC 3 cut(s) 13, 241, 436
Hpy99I CGWCG 3 cut(s) 109, 281, 284
HpyAV CCTTC 2 cut(s) 409, 606
HpyCH4III ACNGT 1 cut(s) 506
HpyCH4IV ACGT 2 cut(s) 249, 279
HpyCH4V TGCA 6 cut(s) 241, 315, 565, 686, 746, 764
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 210, 312, 746
HpyF3I CTNAG 3 cut(s) 187, 231, 498
HpySE526I ACGT 2 cut(s) 249, 279
Hsp92I GRCGYC 4 cut(s) 57, 204, 279, 538
Hsp92II CATG 2 cut(s) 382, 569
HspAI GCGC 4 cut(s) 57, 75, 204, 538
KasI GGCGCC 3 cut(s) 56, 203, 537
KroI GCCGGC 1 cut(s) 792
KroNI GCCGGC 1 cut(s) 794
Ksp22I TGATCA 1 cut(s) 553
KspAI GTTAAC 1 cut(s) 13
Kzo9I GATC 3 cut(s) 163, 393, 553
LmnI GCTCC 4 cut(s) 161, 274, 680, 758
Lsp1109I GCAGC 6 cut(s) 113, 181, 670, 698, 748, 758
LweI GCATC 2 cut(s) 727, 769
MaeI CTAG 1 cut(s) 662
MaeII ACGT 2 cut(s) 249, 279
MaeIII GTNAC 1 cut(s) 632
MalI GATC 3 cut(s) 165, 395, 555
MboI GATC 3 cut(s) 163, 393, 553
MboII GAAGA 4 cut(s) 450, 536, 727, 730
MhlI GDGCHC 2 cut(s) 122, 243
MluCI AATT 4 cut(s) 124, 468, 640, 710
Mly113I GGCGCC 3 cut(s) 57, 204, 538
MlyI GAGTC 2 cut(s) 75, 620
MnlI CCTC 5 cut(s) 194, 196, 229, 361, 514
MroNI GCCGGC 1 cut(s) 792
MroXI GAANNNNTTC 1 cut(s) 595
MseI TTAA 2 cut(s) 12, 372
MspA1I CMGCKG 3 cut(s) 44, 72, 136
MspI CCGG 3 cut(s) 159, 198, 793
MwoI GCNNNNNNNGC 4 cut(s) 203, 210, 312, 746
NaeI GCCGGC 1 cut(s) 794
NarI GGCGCC 3 cut(s) 57, 204, 538
NdeII GATC 3 cut(s) 163, 393, 553
NgoMIV GCCGGC 1 cut(s) 792
NlaIII CATG 2 cut(s) 382, 569
NlaIV GGNNCC 4 cut(s) 58, 205, 420, 539
NmeAIII GCCGAG 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 632
NspI RCATGY 1 cut(s) 569
NspV TTCGAA 1 cut(s) 128
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 255, 273
PdiI GCCGGC 1 cut(s) 794
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 5 cut(s) 18, 130, 455, 489, 819
PflMI CCANNNNNTGG 1 cut(s) 547
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 2 cut(s) 74, 619
PluTI GGCGCC 3 cut(s) 60, 207, 541
PpsI GAGTC 2 cut(s) 74, 619
PspN4I GGNNCC 4 cut(s) 58, 205, 420, 539
PstI CTGCAG 2 cut(s) 688, 766
PstNI CAGNNNCTG 3 cut(s) 91, 194, 822
PvuI CGATCG 1 cut(s) 166
RgaI GCGATCGC 1 cut(s) 166
RsaI GTAC 3 cut(s) 290, 296, 356
RsaNI GTAC 3 cut(s) 289, 295, 355
SaqAI TTAA 2 cut(s) 12, 372
Sau3AI GATC 3 cut(s) 163, 393, 553
SchI GAGTC 2 cut(s) 75, 620
SduI GDGCHC 2 cut(s) 122, 243
SfaAI GCGATCGC 1 cut(s) 166
SfaNI GCATC 2 cut(s) 727, 769
SfcI CTRYAG 2 cut(s) 684, 762
SfoI GGCGCC 3 cut(s) 58, 205, 539
SfuI TTCGAA 1 cut(s) 128
SgfI GCGATCGC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 4 cut(s) 124, 468, 640, 710
SsiI CCGC 7 cut(s) 34, 39, 42, 54, 72, 134, 255
SspDI GGCGCC 3 cut(s) 56, 203, 537
SspMI CTAG 1 cut(s) 662
TaaI ACNGT 1 cut(s) 506
TaiI ACGT 2 cut(s) 252, 282
TaqI TCGA 5 cut(s) 122, 128, 179, 513, 589
TasI AATT 4 cut(s) 124, 468, 640, 710
TauI GCSGC 4 cut(s) 36, 44, 57, 75
TfiI GAWTC 5 cut(s) 18, 130, 455, 489, 819
Tru1I TTAA 2 cut(s) 12, 372
Tru9I TTAA 2 cut(s) 12, 372
TscAI CASTG 3 cut(s) 195, 511, 548
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 6 cut(s) 101, 194, 683, 686, 746, 761
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 3 cut(s) 30, 214, 342
TspRI CASTG 3 cut(s) 195, 511, 548
Van91I CCANNNNNTGG 1 cut(s) 547
VneI GTGCAC 1 cut(s) 239
XapI RAATTY 2 cut(s) 124, 640
XceI RCATGY 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 1 cut(s) 662
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.