Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003258

Physical Location & Seq

Genomic Coordinates Reverse (-)

31659496 .. 31663045

Gene Structure 3550 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1236600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGAGAAGGGCTTCCACTCTCGCCTCACTCCCCCTCCTCTCCCGATCCCTTTCCACCGCCCACGGCGGCGGCGCCGTAACTTCAGCCCCCACCACCTACGGTCTTCTCCAGGTTGCTCTGTACGGGACCACCTCCACCGGTTCTCAGGCTCCTCGTCGATGGCTCGATTCTTTTTCCGGAGGTTCGGGTCGGAAGCTGGCAATCGGCGTCGCCGGAACTCTGGCTTCGGTCGCGGTCGCCACGTCGCTCGCTCCGGAGGTCTATGCCAAAGAGCCGCCTCCGGCTGCGCTTGTTCCGAAGGAAGTTGTGCTTTACCAGTACGAAGCCTGCCCTTTCTGCAACAAAGTTAAAGCATTCTTGGATTACCACGATATACCCTACAAAGTTGTGGAGGTCAACCCACTTAGTAAGAAAGAGATTAAATGGTCTGATTATAAGAAGGTTCCTATACTAATGGTGGACGGAGAGCAGCTGGTAGATTCATCAGCTATAATTGATCAGTTGAATAGCAAAATTGTTCCTGAGAGAGCAGCCGCTTCTTCCTCAGATGACGATGAAGAGAAAAAGTGGCGCCAGTGGGTTGATAATCACTTGGTGCATATGTTGTCACCAAACATATACCGAAATACATCGGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTTAGCTACACAGAAAAAATTTCAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTGTCAAAGAAACTAAAGAAGAAATATAATATTACTGATGAGCGTGCATCCCTGTATGAAGCAGCAGAAACATGGGTTGATGCTCTTAACGGGCGGGACTTCCTTGGGGGCTCCAAACCGAATTTGGCAGACCTTGCTGTTTTTGGGGTGCTAAGACCAATCCGTTATTTGAGGTCGGGTAAAGATATGGTGGAGCACACACGCATCGGTGAATGGTACTCAAGAATGGAGCGTGCCGTGGGAGAGCGTGCAAGGATTAAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0016829 GO:0020037 GO:0033218 GO:0042277 GO:0043167 GO:0043168 GO:0043295 GO:0046906 GO:0048037 GO:0072341 GO:0097159 GO:1900750 GO:1901363 GO:1901681

KEGG Pathways

Metabolic & Signaling

ko00590 ko01100 map00590 map01100

Pfam Domains

Protein Families

GST_C GST_N_3 Glutaredoxin

Protein Analysis

333

Amino Acids

36.7

Weight (kDa)

8.91

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glutaredoxin	PF00462	102 - 156	2.9e-11	Glutaredoxin
GST_N_3	PF13417	104 - 170	2.2e-15	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	109 - 169	1.1e-08	Glutathione S-transferase, N-terminal domain
GST_N_4	PF17172	111 - 202	2.1e-10	Glutathione S-transferase N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003258)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42150
fragaria_vesca	FvH4_2g38150 FvH4_5g17390 FvH4_5g17390
malus_domestica	MD08G1010100.v1.1 MD14G1236600.v1.1
prunus_persica	Prupe.1G362400_v2.0.a1 Prupe.1G362400_v2.0.a1 Prupe.5G233700_v2.0.a1 Prupe.5G233700_v2.0.a1 Prupe.5G233700_v2.0.a1
pyrus_communis	pycom06g20360 pycom08g00850 pycom14g19760
rosa_chinensis	RchiOBHm_Chr6g0303331 RchiOBHm_Chr7g0178351
rosa_laevigata	RLG00000005453 RLG00000011091
rosa_multiflora	Rmu_sc0014842.1_g000009 Rmu_sc0028437.1_g000002 Rmu_ssc0000114.1_g000063
rosa_roxburghii	Rroxscaffold_3G00274990 Rroxscaffold_7G00164860
rosa_rugosa	Rorug06G0320600 Rorug06G0320700 Rorug06G0416200
rosa_samantha	Rh6AG434900 Rh6BG468200 Rh6CG447000 Rh6DG433800 Rh7AG015000 Rh7BG014500 Rh7CG014500 Rh7DG014800
rosa_wichuraiana	Rw0G010210 Rw6G037660 Rw7G001190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	435
AccB1I	GGYRCC	2 cut(s)	71, 570
AccII	CGCG	1 cut(s)	233
AccIII	TCCGGA	2 cut(s)	176, 253
AciI	CCGC	7 cut(s)	57, 66, 69, 233, 275, 534, 826
AclWI	GGATC	1 cut(s)	39
AcsI	RAATTY	2 cut(s)	693, 853
AcuI	CTGAAG	1 cut(s)	66
AcyI	GRCGYC	3 cut(s)	72, 207, 571
AdeI	CACNNNGTG	1 cut(s)	595
AfaI	GTAC	4 cut(s)	122, 320, 728, 950
AgeI	ACCGGT	1 cut(s)	137
AgsI	TTSAA	1 cut(s)	505
AjiI	CACGTC	1 cut(s)	243
AjnI	CCWGG	1 cut(s)	108
AluBI	AGCT	5 cut(s)	196, 472, 488, 681, 722
AluI	AGCT	5 cut(s)	196, 472, 488, 681, 722
Alw21I	GWGCWC	1 cut(s)	930
AlwI	GGATC	1 cut(s)	39
Aor13HI	TCCGGA	2 cut(s)	176, 253
AoxI	GGCC	1 cut(s)	636
ApeKI	GCWGC	6 cut(s)	284, 469, 530, 716, 719, 794
ApoI	RAATTY	2 cut(s)	693, 853
AsiGI	ACCGGT	1 cut(s)	137
Asp700I	GAANNNNTTC	1 cut(s)	10
AspLEI	GCGC	3 cut(s)	74, 289, 573
AspS9I	GGNCC	2 cut(s)	126, 637
AsuHPI	GGTGA	2 cut(s)	600, 953
AvaII	GGWCC	1 cut(s)	126
BanI	GGYRCC	2 cut(s)	71, 570
BanII	GRGCYC	1 cut(s)	845
BbsI	GAAGAC	1 cut(s)	95
Bbv12I	GWGCWC	1 cut(s)	930
BbvI	GCAGC	6 cut(s)	271, 481, 542, 703, 731, 806
BccI	CCATC	1 cut(s)	153
BceAI	ACGGC	3 cut(s)	59, 79, 953
BciT130I	CCWGG	1 cut(s)	110
BclI	TGATCA	1 cut(s)	496
BfmI	CTRYAG	1 cut(s)	717
BfoI	RGCGCY	2 cut(s)	75, 574
Bme1390I	CCNGG	1 cut(s)	110
Bme18I	GGWCC	1 cut(s)	126
BmgBI	CACGTC	1 cut(s)	243
BmgT120I	GGNCC	2 cut(s)	126, 637
BmiI	GGNNCC	6 cut(s)	73, 127, 150, 444, 572, 844
BmrFI	CCNGG	1 cut(s)	110
BmsI	GCATC	3 cut(s)	788, 802, 945
BpiI	GAAGAC	1 cut(s)	95
BpmI	CTGGAG	1 cut(s)	92
BpuEI	CTTGAG	2 cut(s)	662, 937
BsaHI	GRCGYC	3 cut(s)	72, 207, 571
BsaJI	CCNNGG	3 cut(s)	61, 835, 969
BsaWI	WCCGGW	3 cut(s)	137, 176, 253
BsaXI	ACNNNNNCTCC	2 cut(s)	18, 48
Bse118I	RCCGGY	1 cut(s)	137
Bse1I	ACTGG	2 cut(s)	316, 574
Bse3DI	GCAATG	1 cut(s)	673
BseAI	TCCGGA	2 cut(s)	176, 253
BseBI	CCWGG	1 cut(s)	110
BseDI	CCNNGG	3 cut(s)	61, 835, 969
BseGI	GGATG	1 cut(s)	779
BseMI	GCAATG	1 cut(s)	673
BseMII	CTCAG	3 cut(s)	158, 513, 558
BseNI	ACTGG	2 cut(s)	316, 574
BseRI	GAGGAG	2 cut(s)	26, 141
BseXI	GCAGC	6 cut(s)	271, 481, 542, 703, 731, 806
Bsh1236I	CGCG	1 cut(s)	233
Bsh1285I	CGRYCG	2 cut(s)	231, 237
BshFI	GGCC	1 cut(s)	638
BshNI	GGYRCC	2 cut(s)	71, 570
BshTI	ACCGGT	1 cut(s)	137
BsiEI	CGRYCG	2 cut(s)	231, 237
BsiHKAI	GWGCWC	1 cut(s)	930
BsiSI	CCGG	5 cut(s)	138, 177, 213, 254, 281
BslFI	GGGAC	2 cut(s)	139, 842
BsmFI	GGGAC	2 cut(s)	139, 842
BsmI	GAATGC	1 cut(s)	353
BsnI	GGCC	1 cut(s)	638
Bsp1286I	GDGCHC	2 cut(s)	845, 930
Bsp13I	TCCGGA	2 cut(s)	176, 253
Bsp143I	GATC	2 cut(s)	44, 496
BspACI	CCGC	7 cut(s)	57, 66, 69, 233, 275, 534, 826
BspANI	GGCC	1 cut(s)	638
BspCNI	CTCAG	3 cut(s)	157, 514, 557
BspEI	TCCGGA	2 cut(s)	176, 253
BspFNI	CGCG	1 cut(s)	233
BspLI	GGNNCC	6 cut(s)	73, 127, 150, 444, 572, 844
BspMAI	CTGCAG	1 cut(s)	721
BspPI	GGATC	1 cut(s)	39
BspT107I	GGYRCC	2 cut(s)	71, 570
BsrDI	GCAATG	1 cut(s)	673
BsrFI	RCCGGY	1 cut(s)	137
BsrI	ACTGG	2 cut(s)	316, 574
BssAI	RCCGGY	1 cut(s)	137
BssECI	CCNNGG	3 cut(s)	61, 835, 969
BssMI	GATC	2 cut(s)	44, 496
BssNI	GRCGYC	3 cut(s)	72, 207, 571
BssT1I	CCWWGG	1 cut(s)	835
Bst2UI	CCWGG	1 cut(s)	110
Bst4CI	ACNGT	1 cut(s)	101
Bst6I	CTCTTC	1 cut(s)	552
BstACI	GRCGYC	3 cut(s)	72, 207, 571
BstAPI	GCANNNNNTGC	1 cut(s)	866
BstC8I	GCNNGC	6 cut(s)	198, 249, 328, 777, 966, 981
BstDEI	CTNAG	5 cut(s)	144, 404, 522, 544, 884
BstDSI	CCRYGG	2 cut(s)	61, 969
BstF5I	GGATG	1 cut(s)	779
BstFNI	CGCG	1 cut(s)	233
BstH2I	RGCGCY	2 cut(s)	75, 574
BstHHI	GCGC	3 cut(s)	74, 289, 573
BstKTI	GATC	2 cut(s)	47, 499
BstMBI	GATC	2 cut(s)	44, 496
BstMCI	CGRYCG	2 cut(s)	231, 237
BstMWI	GCNNNNNNNGC	4 cut(s)	230, 336, 716, 866
BstNI	CCWGG	1 cut(s)	110
BstSCI	CCNGG	1 cut(s)	108
BstSFI	CTRYAG	1 cut(s)	717
BstUI	CGCG	1 cut(s)	233
BstV1I	GCAGC	6 cut(s)	271, 481, 542, 703, 731, 806
BstV2I	GAAGAC	1 cut(s)	95
BsuRI	GGCC	1 cut(s)	638
BtgI	CCRYGG	2 cut(s)	61, 969
BtrI	CACGTC	1 cut(s)	243
BtsCI	GGATG	1 cut(s)	779
BtsIMutI	CAGTG	2 cut(s)	581, 705
Cac8I	GCNNGC	6 cut(s)	198, 249, 328, 777, 966, 981
CfoI	GCGC	3 cut(s)	74, 289, 573
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	2 cut(s)	126, 637
CseI	GACGC	1 cut(s)	196
Csp6I	GTAC	4 cut(s)	121, 319, 727, 949
CspAI	ACCGGT	1 cut(s)	137
CviAII	CATG	1 cut(s)	804
CviQI	GTAC	4 cut(s)	121, 319, 727, 949
DdeI	CTNAG	5 cut(s)	144, 404, 522, 544, 884
DinI	GGCGCC	2 cut(s)	73, 572
DpnI	GATC	2 cut(s)	46, 498
DpnII	GATC	2 cut(s)	44, 496
DraIII	CACNNNGTG	1 cut(s)	595
Eam1104I	CTCTTC	1 cut(s)	552
EarI	CTCTTC	1 cut(s)	552
Eco130I	CCWWGG	1 cut(s)	835
Eco24I	GRGCYC	1 cut(s)	845
Eco47I	GGWCC	1 cut(s)	126
Eco57I	CTGAAG	1 cut(s)	66
EcoO109I	RGGNCCY	1 cut(s)	637
EcoRII	CCWGG	1 cut(s)	108
EcoT14I	CCWWGG	1 cut(s)	835
EcoT38I	GRGCYC	1 cut(s)	845
EgeI	GGCGCC	2 cut(s)	73, 572
EheI	GGCGCC	2 cut(s)	73, 572
ErhI	CCWWGG	1 cut(s)	835
FaeI	CATG	1 cut(s)	807
FalI	AAGNNNNNCTT	2 cut(s)	294, 326
FaqI	GGGAC	2 cut(s)	139, 842
FatI	CATG	1 cut(s)	803
FauI	CCCGC	1 cut(s)	819
FauNDI	CATATG	1 cut(s)	600
FbaI	TGATCA	1 cut(s)	496
FokI	GGATG	1 cut(s)	766
FriOI	GRGCYC	1 cut(s)	845
GlaI	GCGC	3 cut(s)	73, 288, 572
GsuI	CTGGAG	1 cut(s)	92
HaeII	RGCGCY	2 cut(s)	75, 574
HaeIII	GGCC	1 cut(s)	638
HapII	CCGG	5 cut(s)	138, 177, 213, 254, 281
HgaI	GACGC	1 cut(s)	196
HhaI	GCGC	3 cut(s)	74, 289, 573
Hin1I	GRCGYC	3 cut(s)	72, 207, 571
Hin1II	CATG	1 cut(s)	807
Hin6I	GCGC	3 cut(s)	72, 287, 571
HinP1I	GCGC	3 cut(s)	72, 287, 571
HincII	GTYRAC	1 cut(s)	397
HindII	GTYRAC	1 cut(s)	397
HinfI	GANTC	3 cut(s)	167, 479, 644
HpaII	CCGG	5 cut(s)	138, 177, 213, 254, 281
HphI	GGTGA	2 cut(s)	600, 953
Hpy166II	GTNNAC	2 cut(s)	397, 460
Hpy188I	TCNGA	5 cut(s)	192, 297, 430, 547, 634
Hpy188III	TCNNGA	5 cut(s)	42, 177, 254, 521, 954
Hpy8I	GTNNAC	2 cut(s)	397, 460
Hpy99I	CGWCG	3 cut(s)	159, 212, 247
HpyAV	CCTTC	2 cut(s)	292, 433
HpyCH4III	ACNGT	1 cut(s)	101
HpyCH4IV	ACGT	1 cut(s)	242
HpyCH4V	TGCA	5 cut(s)	339, 598, 719, 779, 983
HpyF10VI	GCNNNNNNNGC	4 cut(s)	230, 336, 716, 866
HpyF3I	CTNAG	5 cut(s)	144, 404, 522, 544, 884
HpySE526I	ACGT	1 cut(s)	242
Hsp92I	GRCGYC	3 cut(s)	72, 207, 571
Hsp92II	CATG	1 cut(s)	807
HspAI	GCGC	3 cut(s)	72, 287, 571
KasI	GGCGCC	2 cut(s)	71, 570
Kpn2I	TCCGGA	2 cut(s)	176, 253
Ksp22I	TGATCA	1 cut(s)	496
Kzo9I	GATC	2 cut(s)	44, 496
LmnI	GCTCC	5 cut(s)	154, 256, 848, 925, 961
Lsp1109I	GCAGC	6 cut(s)	271, 481, 542, 703, 731, 806
LweI	GCATC	3 cut(s)	788, 802, 945
MaeII	ACGT	1 cut(s)	242
MaeIII	GTNAC	2 cut(s)	76, 606
MalI	GATC	2 cut(s)	46, 498
MboI	GATC	2 cut(s)	44, 496
MboII	GAAGA	4 cut(s)	95, 531, 569, 763
MhlI	GDGCHC	2 cut(s)	845, 930
MluCI	AATT	5 cut(s)	492, 513, 673, 693, 853
Mly113I	GGCGCC	2 cut(s)	72, 571
MlyI	GAGTC	1 cut(s)	653
MmeI	TCCRAC	1 cut(s)	170
MroI	TCCGGA	2 cut(s)	176, 253
MroXI	GAANNNNTTC	1 cut(s)	10
MseI	TTAA	4 cut(s)	348, 420, 819, 990
MspA1I	CMGCKG	1 cut(s)	472
MspI	CCGG	5 cut(s)	138, 177, 213, 254, 281
MspR9I	CCNGG	1 cut(s)	110
Mva1269I	GAATGC	1 cut(s)	353
MvaI	CCWGG	1 cut(s)	110
MvnI	CGCG	1 cut(s)	233
MwoI	GCNNNNNNNGC	4 cut(s)	230, 336, 716, 866
NarI	GGCGCC	2 cut(s)	72, 571
NdeI	CATATG	1 cut(s)	600
NdeII	GATC	2 cut(s)	44, 496
NlaIII	CATG	1 cut(s)	807
NlaIV	GGNNCC	6 cut(s)	73, 127, 150, 444, 572, 844
NmuCI	GTSAC	1 cut(s)	606
PctI	GAATGC	1 cut(s)	353
PdmI	GAANNNNTTC	1 cut(s)	10
PfeI	GAWTC	2 cut(s)	167, 479
PinAI	ACCGGT	1 cut(s)	137
PleI	GAGTC	1 cut(s)	652
PluTI	GGCGCC	2 cut(s)	75, 574
PpsI	GAGTC	1 cut(s)	652
PsiI	TTATAA	1 cut(s)	435
Psp6I	CCWGG	1 cut(s)	108
PspGI	CCWGG	1 cut(s)	108
PspN4I	GGNNCC	6 cut(s)	73, 127, 150, 444, 572, 844
PspPI	GGNCC	2 cut(s)	126, 637
PstI	CTGCAG	1 cut(s)	721
PvuII	CAGCTG	1 cut(s)	472
RsaI	GTAC	4 cut(s)	122, 320, 728, 950
RsaNI	GTAC	4 cut(s)	121, 319, 727, 949
SaqAI	TTAA	4 cut(s)	348, 420, 819, 990
Sau3AI	GATC	2 cut(s)	44, 496
Sau96I	GGNCC	2 cut(s)	126, 637
SchI	GAGTC	1 cut(s)	653
ScrFI	CCNGG	1 cut(s)	110
SduI	GDGCHC	2 cut(s)	845, 930
SfaNI	GCATC	3 cut(s)	788, 802, 945
SfcI	CTRYAG	1 cut(s)	717
SfoI	GGCGCC	2 cut(s)	73, 572
SinI	GGWCC	1 cut(s)	126
SmlI	CTYRAG	2 cut(s)	641, 952
SmoI	CTYRAG	2 cut(s)	641, 952
Sse9I	AATT	5 cut(s)	492, 513, 673, 693, 853
SsiI	CCGC	7 cut(s)	57, 66, 69, 233, 275, 534, 826
SspDI	GGCGCC	2 cut(s)	71, 570
SspI	AATATT	1 cut(s)	763
StyD4I	CCNGG	1 cut(s)	108
StyI	CCWWGG	1 cut(s)	835
TaaI	ACNGT	1 cut(s)	101
TaiI	ACGT	1 cut(s)	245
TaqI	TCGA	2 cut(s)	157, 165
TaqII	GACCGA	1 cut(s)	217
TasI	AATT	5 cut(s)	492, 513, 673, 693, 853
TatI	WGTACW	1 cut(s)	726
TauI	GCSGC	4 cut(s)	69, 72, 277, 536
TfiI	GAWTC	2 cut(s)	167, 479
Tru1I	TTAA	4 cut(s)	348, 420, 819, 990
Tru9I	TTAA	4 cut(s)	348, 420, 819, 990
TscAI	CASTG	2 cut(s)	581, 705
TseFI	GTSAC	1 cut(s)	606
TseI	GCWGC	6 cut(s)	284, 469, 530, 716, 719, 794
Tsp45I	GTSAC	1 cut(s)	606
TspDTI	ATGAA	3 cut(s)	471, 570, 804
TspGWI	ACGGA	2 cut(s)	477, 884
TspRI	CASTG	2 cut(s)	581, 705
VpaK11BI	GGWCC	1 cut(s)	126
XapI	RAATTY	2 cut(s)	693, 853
XcmI	CCANNNNNNNNNTGG	1 cut(s)	853
XmnI	GAANNNNTTC	1 cut(s)	10

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.