FvH4_2g38462

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010516
Physical Location & Seq
Forward (+)
27820706 .. 27824291
3586 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38462.t1

Sequence Viewer

Length: 588 bp
ATGACTTGCAGACGCAAGAGGCAACGGCCAGGGTTTTGGGGTCAAGATGAGGACCGGGGCGGTGGTTATTATTTATGGGGAGGGGTGGACGACACGCACGCGGAGGGAAGTGTTGCAATGCACGGTTGTGAGTCGCCACGAGATGCATGTGTCTGGTCGACGACTCTGACCGGAATACACGTGCCGGTGGTAGCGGAGGTTCTAGAGGAGTCGGATCTGGGCCGGAGAGTGGGAGAGGTTGAGGGCAACGAGGGTGGTGGCGTTGAAGAGGTGGCCGTAGAGAAGAGAGAAGAAGAATGGGCTGTTCTTGGACAAGTGTGGAGGGAGGCGGCCAAATGGGTTGTTTCCAAGGTTTTGATTGATCTTACAGAAAATAGAGGCAATGATGATTTTGCAGTTGTTTTCCTGATGACGTCGAACTGGAGCCAGATCCTAAGAGACGTTGCGGCGGCGGCGGTGGTCTTCTCCGGCGAGAAGATGGAGAGCAAGTTGTTGCGGGAGATGGAGACCTTGGGAGAGCCAGAAACGGCAAGGCCGATGGAACCGGTTCGGATCAGAACAGATGATTTCATCGCTGGAGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.62

Weight (kDa)

4.69

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
AccI GTMKAC 1 cut(s) 158
AccII CGCG 1 cut(s) 101
AciI CCGC 9 cut(s) 60, 101, 194, 329, 446, 449, 452, 455, 496
AclWI GGATC 3 cut(s) 222, 424, 560
AcoI YGGCCR 3 cut(s) 26, 273, 330
AcvI CACGTG 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 229
AflIII ACRYGT 1 cut(s) 178
AgeI ACCGGT 1 cut(s) 544
AgsI TTSAA 1 cut(s) 266
AjnI CCWGG 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 432, 500
AlwI GGATC 3 cut(s) 222, 424, 560
AoxI GGCC 5 cut(s) 26, 220, 273, 330, 533
AsiGI ACCGGT 1 cut(s) 544
Asp700I GAANNNNTTC 1 cut(s) 546
AspS9I GGNCC 2 cut(s) 52, 220
AsuC2I CCSGG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 52
BauI CACGAG 1 cut(s) 138
BbrPI CACGTG 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 454
BccI CCATC 3 cut(s) 472, 496, 532
BceAI ACGGC 3 cut(s) 41, 260, 543
BciT130I CCWGG 1 cut(s) 30
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 2 cut(s) 432, 500
BfaI CTAG 1 cut(s) 203
BisI GCNGC 4 cut(s) 330, 447, 450, 453
BlsI GCNGC 4 cut(s) 331, 448, 451, 454
Bme1390I CCNGG 2 cut(s) 30, 56
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 220
BmiI GGNNCC 2 cut(s) 425, 543
BmrFI CCNGG 2 cut(s) 30, 56
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 442
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 413
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 4 cut(s) 29, 55, 348, 510
BsaWI WCCGGW 2 cut(s) 170, 544
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse118I RCCGGY 2 cut(s) 184, 544
Bse1I ACTGG 1 cut(s) 425
Bse3DI GCAATG 2 cut(s) 123, 388
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 4 cut(s) 29, 55, 348, 510
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 2 cut(s) 123, 388
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 101
BshFI GGCC 5 cut(s) 28, 222, 275, 332, 535
BshTI ACCGGT 1 cut(s) 544
BsiSI CCGG 6 cut(s) 55, 171, 185, 223, 468, 545
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 432, 500
BsmBI CGTCTC 1 cut(s) 432
BsnI GGCC 5 cut(s) 28, 222, 275, 332, 535
Bso31I GGTCTC 1 cut(s) 500
Bsp143I GATC 4 cut(s) 214, 361, 429, 552
BspACI CCGC 9 cut(s) 60, 101, 194, 329, 446, 449, 452, 455, 496
BspANI GGCC 5 cut(s) 28, 222, 275, 332, 535
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 2 cut(s) 425, 543
BspPI GGATC 3 cut(s) 222, 424, 560
BspTNI GGTCTC 1 cut(s) 500
BsrDI GCAATG 2 cut(s) 123, 388
BsrFI RCCGGY 2 cut(s) 184, 544
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 2 cut(s) 184, 544
BssECI CCNNGG 4 cut(s) 29, 55, 348, 510
BssMI GATC 4 cut(s) 214, 361, 429, 552
BssNI GRCGYC 1 cut(s) 413
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 348, 510
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 261, 278
BstACI GRCGYC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 434
BstFNI CGCG 1 cut(s) 101
BstKTI GATC 4 cut(s) 217, 364, 432, 555
BstMAI GTCTC 2 cut(s) 432, 500
BstMBI GATC 4 cut(s) 214, 361, 429, 552
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 150
BstSCI CCNGG 2 cut(s) 28, 54
BstUI CGCG 1 cut(s) 101
BstV2I GAAGAC 1 cut(s) 454
BstX2I RGATCY 2 cut(s) 214, 429
BstXI CCANNNNNNTGG 1 cut(s) 36
BstYI RGATCY 2 cut(s) 214, 429
BsuRI GGCC 5 cut(s) 28, 222, 275, 332, 535
BtgZI GCGATG 1 cut(s) 556
Cac8I GCNNGC 1 cut(s) 99
Cfr10I RCCGGY 2 cut(s) 184, 544
Cfr13I GGNCC 2 cut(s) 52, 220
CseI GACGC 1 cut(s) 21
CspAI ACCGGT 1 cut(s) 544
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 1 cut(s) 147
CviJI RGCY 8 cut(s) 28, 222, 275, 302, 332, 426, 520, 535
CviKI_1 RGCY 8 cut(s) 28, 222, 275, 302, 332, 426, 520, 535
DdeI CTNAG 1 cut(s) 434
DpnI GATC 4 cut(s) 216, 363, 431, 554
DpnII GATC 4 cut(s) 214, 361, 429, 552
EaeI YGGCCR 3 cut(s) 26, 273, 330
Eam1104I CTCTTC 2 cut(s) 261, 278
EarI CTCTTC 2 cut(s) 261, 278
Eco130I CCWWGG 2 cut(s) 348, 510
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 52
Eco72I CACGTG 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 348, 510
EcoT22I ATGCAT 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 348, 510
Esp3I CGTCTC 1 cut(s) 432
FaeI CATG 1 cut(s) 150
FaiI YATR 2 cut(s) 76, 148
FatI CATG 1 cut(s) 146
FauI CCCGC 1 cut(s) 489
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 4 cut(s) 330, 447, 450, 453
Fsp4HI GCNGC 4 cut(s) 330, 447, 450, 453
FspBI CTAG 1 cut(s) 203
GluI GCNGC 4 cut(s) 330, 447, 450, 453
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 5 cut(s) 28, 222, 275, 332, 535
HapII CCGG 6 cut(s) 55, 171, 185, 223, 468, 545
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 1 cut(s) 150
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 3 cut(s) 131, 163, 209
HpaII CCGG 6 cut(s) 55, 171, 185, 223, 468, 545
Hpy166II GTNNAC 2 cut(s) 88, 159
Hpy188I TCNGA 4 cut(s) 168, 214, 552, 557
Hpy188III TCNNGA 3 cut(s) 44, 203, 406
Hpy8I GTNNAC 2 cut(s) 88, 159
Hpy99I CGWCG 2 cut(s) 163, 418
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 3 cut(s) 180, 413, 441
HpyCH4V TGCA 5 cut(s) 9, 116, 121, 146, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 1 cut(s) 434
HpySE526I ACGT 3 cut(s) 180, 413, 441
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 1 cut(s) 150
Kzo9I GATC 4 cut(s) 214, 361, 429, 552
LmnI GCTCC 1 cut(s) 423
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 203
MaeII ACGT 3 cut(s) 180, 413, 441
MalI GATC 4 cut(s) 216, 363, 431, 554
MboI GATC 4 cut(s) 214, 361, 429, 552
MboII GAAGA 6 cut(s) 278, 295, 302, 305, 454, 487
MflI RGATCY 2 cut(s) 214, 429
MlyI GAGTC 3 cut(s) 140, 157, 218
MmeI TCCRAC 1 cut(s) 192
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 6 cut(s) 55, 171, 185, 223, 468, 545
MspR9I CCNGG 2 cut(s) 30, 56
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 452
NciI CCSGG 1 cut(s) 56
NdeII GATC 4 cut(s) 214, 361, 429, 552
NlaIII CATG 1 cut(s) 150
NlaIV GGNNCC 2 cut(s) 425, 543
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 546
PinAI ACCGGT 1 cut(s) 544
PkrI GCNGC 4 cut(s) 331, 448, 451, 454
PleI GAGTC 3 cut(s) 139, 157, 217
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 3 cut(s) 139, 157, 217
Ppu21I YACGTR 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 28
PspCI CACGTG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 425, 543
PspPI GGNCC 2 cut(s) 52, 220
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PsuI RGATCY 2 cut(s) 214, 429
RseI CAYNNNNRTG 1 cut(s) 126
SalI GTCGAC 1 cut(s) 157
SatI GCNGC 4 cut(s) 330, 447, 450, 453
Sau3AI GATC 4 cut(s) 214, 361, 429, 552
Sau96I GGNCC 2 cut(s) 52, 220
SchI GAGTC 3 cut(s) 140, 157, 218
ScrFI CCNGG 2 cut(s) 30, 56
SetI ASST 8 cut(s) 183, 201, 240, 273, 354, 416, 444, 512
SfaNI GCATC 1 cut(s) 133
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 126
SsiI CCGC 9 cut(s) 60, 101, 194, 329, 446, 449, 452, 455, 496
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 28, 54
StyI CCWWGG 2 cut(s) 348, 510
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 3 cut(s) 183, 416, 444
TaqI TCGA 2 cut(s) 158, 416
TauI GCSGC 4 cut(s) 332, 449, 452, 455
TspDTI ATGAA 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 52
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.