FvH4_3g34152

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010516
Physical Location & Seq
Forward (+)
29560241 .. 29562965
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34152.t1

Sequence Viewer

Length: 810 bp
ATGATGGTGTTGCAGAGGCGCGCTCGGGGCTCGGCAGGAGTTCTATCCGAGCTGGATCGTGGCTGGGGAAGGGCGTTGCTTACAGGCGTGCGCCAGGGTCTGTGGAGGAGGCCGGTGTTCTCATTCTCTTTAGTGGAGGAGGCCGTCAGGGACTGCGACTTGTTCTCTTCGGTGAAGGTGGGTGCCCAGAGAAGAAATATAGTAACTGGTTACAGAGGAAATTCCGGTGACCGAAATCCGACGGTCGGGGAAGTTCCGGTGATCGGAATCCGGCAGCCGGTGACTGGAGTCCGGCGGCGTGTGACCGGAGTCCGAGGTTCCGACCAAGAAACTCATGGACAAGATAATGAGAAGATGAAGCAATGGAGGAATGCTATAGAAGAAGTCGCTGGATTGTCTGGCTGGAGAGGAGACGACGGGAACGAGGGCGGCGATCATGATTGTGGGGTTCTGCGGTTAGAATTGACAGCACCGGCGGGGGCACCGGTGATAACGCGGTCGAGACTTGGAGAGGAGCGTCGTCGAGGAAGGGAAGGAGGGGACGGCGTACTTGGAGAGAGAGACAGAACAAAGCGAACGGGAACGGCGGGGAACGACCATCCACTTCTGTTCTATTTCTATTTCTTTCTCGCCGTCGTAATACGGGTCGTGGTTGCGGGTCGACGGTCGTCTATCCGGGTCTCAGAAGTTCGGGCCTTCGTGCGTGTGATCTGGTGCACCTGTTCACATTCAGCATTAATACGTATATTCAATCTAAATGGCTCAGATGAAGCCCTAAAGAAGCTAAACGGTCCAGATCGCACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

270

Amino Acids

29.77

Weight (kDa)

10.52

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 182, 481
AccI GTMKAC 1 cut(s) 661
AccII CGCG 2 cut(s) 21, 496
AciI CCGC 7 cut(s) 295, 429, 454, 476, 496, 587, 656
AclWI GGATC 1 cut(s) 63
AcsI RAATTY 1 cut(s) 220
AfaI GTAC 1 cut(s) 549
AfiI CCNNNNNNNGG 4 cut(s) 245, 263, 277, 284
AgeI ACCGGT 1 cut(s) 484
AgsI TTSAA 1 cut(s) 751
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 2 cut(s) 52, 784
AluI AGCT 2 cut(s) 52, 784
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 4 cut(s) 405, 496, 555, 685
Alw44I GTGCAC 1 cut(s) 715
AlwI GGATC 1 cut(s) 63
AlwNI CAGNNNCTG 2 cut(s) 100, 153
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 3 cut(s) 110, 141, 693
ApaLI GTGCAC 1 cut(s) 715
ApeKI GCWGC 1 cut(s) 274
ApoI RAATTY 1 cut(s) 220
AseI ATTAAT 1 cut(s) 737
AsiGI ACCGGT 1 cut(s) 484
AspLEI GCGC 3 cut(s) 21, 23, 93
AspS9I GGNCC 2 cut(s) 693, 791
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 5 cut(s) 184, 239, 271, 292, 499
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 791
BaeGI GKGCMC 3 cut(s) 187, 484, 719
BanI GGYRCC 2 cut(s) 182, 481
BanII GRGCYC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 719
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 606
BceAI ACGGC 4 cut(s) 128, 559, 600, 617
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 4 cut(s) 405, 496, 555, 685
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 3 cut(s) 275, 296, 430
BlsI GCNGC 3 cut(s) 276, 297, 431
Bme1390I CCNGG 2 cut(s) 95, 677
Bme18I GGWCC 1 cut(s) 791
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 2 cut(s) 693, 791
BmiI GGNNCC 3 cut(s) 184, 319, 483
BmrFI CCNGG 2 cut(s) 95, 677
BoxI GACNNNNGTC 3 cut(s) 287, 308, 667
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 306, 424
BpuMI CCSGG 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 94, 313
BsaWI WCCGGW 4 cut(s) 224, 256, 305, 484
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 263, 277, 284
Bse118I RCCGGY 4 cut(s) 112, 277, 472, 484
Bse1I ACTGG 2 cut(s) 211, 289
Bse3DI GCAATG 1 cut(s) 368
Bse8I GATNNNNATC 1 cut(s) 266
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 94, 313
BseGI GGATG 1 cut(s) 598
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 4 cut(s) 245, 263, 277, 284
BseMI GCAATG 1 cut(s) 368
BseMII CTCAG 2 cut(s) 696, 777
BseNI ACTGG 2 cut(s) 211, 289
BsePI GCGCGC 1 cut(s) 19
BseRI GAGGAG 4 cut(s) 121, 152, 423, 527
BseSI GKGCMC 3 cut(s) 187, 484, 719
BseXI GCAGC 1 cut(s) 286
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 2 cut(s) 21, 496
Bsh1285I CGRYCG 3 cut(s) 246, 500, 668
BshFI GGCC 3 cut(s) 112, 143, 695
BshNI GGYRCC 2 cut(s) 182, 481
BshTI ACCGGT 1 cut(s) 484
BsiEI CGRYCG 3 cut(s) 246, 500, 668
BsiHKAI GWGCWC 1 cut(s) 719
BsiHKCI CYCGRG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 164, 554
BslI CCNNNNNNNGG 4 cut(s) 245, 263, 277, 284
BsmAI GTCTC 4 cut(s) 405, 496, 555, 685
BsmBI CGTCTC 1 cut(s) 405
BsmFI GGGAC 2 cut(s) 164, 554
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 3 cut(s) 112, 143, 695
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 32, 187, 484, 719
Bsp143I GATC 5 cut(s) 55, 261, 433, 708, 796
BspACI CCGC 7 cut(s) 295, 429, 454, 476, 496, 587, 656
BspANI GGCC 3 cut(s) 112, 143, 695
BspCNI CTCAG 2 cut(s) 695, 776
BspFNI CGCG 2 cut(s) 21, 496
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 184, 319, 483
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 2 cut(s) 182, 481
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 4 cut(s) 112, 277, 472, 484
BsrI ACTGG 2 cut(s) 211, 289
BssAI RCCGGY 4 cut(s) 112, 277, 472, 484
BssECI CCNNGG 2 cut(s) 94, 313
BssHII GCGCGC 1 cut(s) 19
BssMI GATC 5 cut(s) 55, 261, 433, 708, 796
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 244, 666, 791
Bst6I CTCTTC 1 cut(s) 172
BstBAI YACGTR 1 cut(s) 743
BstC8I GCNNGC 2 cut(s) 21, 89
BstDEI CTNAG 2 cut(s) 682, 763
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 2 cut(s) 21, 496
BstHHI GCGC 3 cut(s) 21, 23, 93
BstKTI GATC 5 cut(s) 58, 264, 436, 711, 799
BstMAI GTCTC 4 cut(s) 405, 496, 555, 685
BstMBI GATC 5 cut(s) 55, 261, 433, 708, 796
BstMCI CGRYCG 3 cut(s) 246, 500, 668
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNI CCWGG 1 cut(s) 95
BstPAI GACNNNNGTC 3 cut(s) 287, 308, 667
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 93, 675
BstSFI CTRYAG 1 cut(s) 375
BstSLI GKGCMC 3 cut(s) 187, 484, 719
BstSNI TACGTA 1 cut(s) 743
BstUI CGCG 2 cut(s) 21, 496
BstV1I GCAGC 1 cut(s) 286
BsuRI GGCC 3 cut(s) 112, 143, 695
BtsCI GGATG 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 21, 89
CaiI CAGNNNCTG 2 cut(s) 100, 153
CciI TCATGA 1 cut(s) 436
CfoI GCGC 3 cut(s) 21, 23, 93
Cfr10I RCCGGY 4 cut(s) 112, 277, 472, 484
Cfr13I GGNCC 2 cut(s) 693, 791
CseI GACGC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 548
CspAI ACCGGT 1 cut(s) 484
CviAII CATG 2 cut(s) 335, 437
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 2 cut(s) 682, 763
DpnI GATC 5 cut(s) 57, 263, 435, 710, 798
DpnII GATC 5 cut(s) 55, 261, 433, 708, 796
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 791
Eco88I CYCGRG 1 cut(s) 24
Eco91I GGTNACC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 32
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 2 cut(s) 338, 440
FaiI YATR 5 cut(s) 200, 336, 377, 438, 746
FaqI GGGAC 2 cut(s) 164, 554
FatI CATG 2 cut(s) 334, 436
FauI CCCGC 3 cut(s) 469, 580, 649
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 3 cut(s) 275, 296, 430
FokI GGATG 1 cut(s) 585
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 3 cut(s) 275, 296, 430
GlaI GCGC 3 cut(s) 20, 22, 92
GluI GCNGC 3 cut(s) 275, 296, 430
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 2 cut(s) 306, 424
HaeIII GGCC 3 cut(s) 112, 143, 695
HgaI GACGC 1 cut(s) 506
HhaI GCGC 3 cut(s) 21, 23, 93
Hin1II CATG 2 cut(s) 338, 440
Hin6I GCGC 3 cut(s) 19, 21, 91
HinP1I GCGC 3 cut(s) 19, 21, 91
HincII GTYRAC 1 cut(s) 662
HindII GTYRAC 1 cut(s) 662
HinfI GANTC 3 cut(s) 267, 288, 309
HphI GGTGA 5 cut(s) 184, 239, 271, 292, 499
Hpy166II GTNNAC 3 cut(s) 662, 717, 725
Hpy188I TCNGA 7 cut(s) 49, 240, 266, 314, 322, 685, 766
Hpy188III TCNNGA 3 cut(s) 437, 501, 794
Hpy8I GTNNAC 3 cut(s) 662, 717, 725
Hpy99I CGWCG 6 cut(s) 244, 419, 522, 525, 638, 666
HpyAV CCTTC 5 cut(s) 63, 169, 522, 527, 706
HpyCH4III ACNGT 3 cut(s) 244, 666, 791
HpyCH4IV ACGT 1 cut(s) 742
HpyCH4V TGCA 2 cut(s) 13, 717
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpyF3I CTNAG 2 cut(s) 682, 763
HpySE526I ACGT 1 cut(s) 742
Hsp92II CATG 2 cut(s) 338, 440
HspAI GCGC 3 cut(s) 19, 21, 91
Kzo9I GATC 5 cut(s) 55, 261, 433, 708, 796
LmnI GCTCC 1 cut(s) 514
Lsp1109I GCAGC 1 cut(s) 286
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 5 cut(s) 202, 209, 227, 280, 301
MalI GATC 5 cut(s) 57, 263, 435, 710, 798
MboI GATC 5 cut(s) 55, 261, 433, 708, 796
MboII GAAGA 4 cut(s) 159, 204, 364, 392
MhlI GDGCHC 4 cut(s) 32, 187, 484, 719
MluCI AATT 2 cut(s) 220, 461
MlyI GAGTC 2 cut(s) 297, 318
MmeI TCCRAC 2 cut(s) 263, 345
MseI TTAA 1 cut(s) 737
MslI CAYNNNNRTG 1 cut(s) 441
MspR9I CCNGG 2 cut(s) 95, 677
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 2 cut(s) 21, 496
MwoI GCNNNNNNNGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 677
NdeII GATC 5 cut(s) 55, 261, 433, 708, 796
NlaIII CATG 2 cut(s) 338, 440
NlaIV GGNNCC 3 cut(s) 184, 319, 483
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 3 cut(s) 227, 280, 301
PagI TCATGA 1 cut(s) 436
PauI GCGCGC 1 cut(s) 19
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 429
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 1 cut(s) 267
PinAI ACCGGT 1 cut(s) 484
PkrI GCNGC 3 cut(s) 276, 297, 431
PleI GAGTC 2 cut(s) 296, 317
PpsI GAGTC 2 cut(s) 296, 317
Ppu21I YACGTR 1 cut(s) 743
PshAI GACNNNNGTC 3 cut(s) 287, 308, 667
PshBI ATTAAT 1 cut(s) 737
Psp6I CCWGG 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 227
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 3 cut(s) 184, 319, 483
PspPI GGNCC 2 cut(s) 693, 791
PstNI CAGNNNCTG 2 cut(s) 100, 153
PteI GCGCGC 1 cut(s) 19
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 660
SaqAI TTAA 1 cut(s) 737
SatI GCNGC 3 cut(s) 275, 296, 430
Sau3AI GATC 5 cut(s) 55, 261, 433, 708, 796
Sau96I GGNCC 2 cut(s) 693, 791
SchI GAGTC 2 cut(s) 297, 318
ScrFI CCNGG 2 cut(s) 95, 677
SduI GDGCHC 4 cut(s) 32, 187, 484, 719
SetI ASST 6 cut(s) 54, 180, 319, 722, 745, 786
SfcI CTRYAG 1 cut(s) 375
SgrAI CRCCGGYG 2 cut(s) 472, 484
SinI GGWCC 1 cut(s) 791
SmiMI CAYNNNNRTG 1 cut(s) 441
SnaBI TACGTA 1 cut(s) 743
Sse9I AATT 2 cut(s) 220, 461
SsiI CCGC 7 cut(s) 295, 429, 454, 476, 496, 587, 656
StyD4I CCNGG 2 cut(s) 93, 675
TaaI ACNGT 3 cut(s) 244, 666, 791
TaiI ACGT 1 cut(s) 745
TaqI TCGA 3 cut(s) 500, 523, 661
TaqII GACCGA 1 cut(s) 246
TasI AATT 2 cut(s) 220, 461
TauI GCSGC 2 cut(s) 298, 432
TfiI GAWTC 1 cut(s) 267
Tru1I TTAA 1 cut(s) 737
Tru9I TTAA 1 cut(s) 737
TseFI GTSAC 3 cut(s) 227, 280, 301
TseI GCWGC 1 cut(s) 274
Tsp45I GTSAC 3 cut(s) 227, 280, 301
TspDTI ATGAA 2 cut(s) 371, 783
VneI GTGCAC 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 791
VspI ATTAAT 1 cut(s) 737
XapI RAATTY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.