FvH4_6g13191

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010516
Physical Location & Seq
Forward (+)
8043005 .. 8045903
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13191.t1

Sequence Viewer

Length: 450 bp
ATGGGTCTGAGGTTCAATCCTATAGACCACGAACTCGTCGATTACTATCTCCAATCTCGGATCTCTCTTGGTGACAGCTTCCACTCCGATTTCGTCTCCGAATGTCCTGATTTTATGGCGAAAATGAGCCATGGGTTGTTTGGCAAATGTACGGCGGCGAGTGAGATGCGCCAGGGTGTCCTCACCGCCGGTGGAGGTGGGTGGGTGGAGGTGCGACGGTGGAGGAGGCCGGTGTGCTCACCGTCGGGGAAGGTGAATGCCCAGAGAAGAAATTTGGGTGCCCAGTTCAATAACTTAGTTACCGGTGACCGGAGAAAAATTGAGTTATTGGGGATCAGTAACCGAGTTACCGGTGACCGGGGAAATCCGGCAAGCAAAGTCCGGCGACCGGTGACCGGAGTCCGGCGGCTGTTGACGGGAGTCCGGCGGCCGATGACCGGAGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.57

Weight (kDa)

11.15

Isoelectric Point (pI)

69.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 4 cut(s) 155, 186, 406, 427
AclWI GGATC 2 cut(s) 68, 341
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 1 cut(s) 271
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 7 cut(s) 309, 357, 388, 395, 402, 437, 444
AgeI ACCGGT 3 cut(s) 302, 350, 388
AgsI TTSAA 2 cut(s) 16, 289
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 1 cut(s) 78
AluI AGCT 1 cut(s) 78
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 2 cut(s) 68, 341
AoxI GGCC 2 cut(s) 227, 428
ApoI RAATTY 1 cut(s) 271
AsiGI ACCGGT 3 cut(s) 302, 350, 388
AspLEI GCGC 1 cut(s) 171
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 7 cut(s) 83, 175, 231, 265, 317, 365, 403
BaeGI GKGCMC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 168
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 100
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 3 cut(s) 156, 407, 428
BlsI GCNGC 3 cut(s) 157, 408, 429
Bme1390I CCNGG 2 cut(s) 173, 359
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 2 cut(s) 173, 359
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 156
BmuI ACTGGG 1 cut(s) 277
BoxI GACNNNNGTC 2 cut(s) 398, 419
BpuMI CCSGG 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 130, 172, 358
BsaWI WCCGGW 6 cut(s) 302, 309, 350, 388, 395, 437
Bsc4I CCNNNNNNNGG 7 cut(s) 309, 357, 388, 395, 402, 437, 444
Bse118I RCCGGY 5 cut(s) 188, 229, 302, 350, 388
Bse1I ACTGG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 130, 172, 358
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 7 cut(s) 309, 357, 388, 395, 402, 437, 444
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 238
BseSI GKGCMC 1 cut(s) 283
BseX3I CGGCCG 1 cut(s) 428
Bsh1285I CGRYCG 2 cut(s) 389, 431
BshFI GGCC 2 cut(s) 229, 430
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 3 cut(s) 302, 350, 388
BsiEI CGRYCG 2 cut(s) 389, 431
BsiHKAI GWGCWC 1 cut(s) 239
BslI CCNNNNNNNGG 7 cut(s) 309, 357, 388, 395, 402, 437, 444
BsmAI GTCTC 1 cut(s) 100
BsmBI CGTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 229, 430
Bsp1286I GDGCHC 2 cut(s) 239, 283
Bsp143I GATC 2 cut(s) 60, 333
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 4 cut(s) 155, 186, 406, 427
BspANI GGCC 2 cut(s) 229, 430
BspLI GGNNCC 1 cut(s) 280
BspPI GGATC 2 cut(s) 68, 341
BspT107I GGYRCC 1 cut(s) 278
BsrFI RCCGGY 5 cut(s) 188, 229, 302, 350, 388
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 5 cut(s) 188, 229, 302, 350, 388
BssECI CCNNGG 3 cut(s) 130, 172, 358
BssMI GATC 2 cut(s) 60, 333
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 2 cut(s) 219, 243
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 2 cut(s) 8, 295
BstDSI CCRYGG 1 cut(s) 130
BstEII GGTNACC 3 cut(s) 305, 353, 391
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 63, 336
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 60, 333
BstMCI CGRYCG 2 cut(s) 389, 431
BstNI CCWGG 1 cut(s) 173
BstPAI GACNNNNGTC 2 cut(s) 398, 419
BstPI GGTNACC 3 cut(s) 305, 353, 391
BstSCI CCNGG 2 cut(s) 171, 357
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 283
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 428
BsuRI GGCC 2 cut(s) 229, 430
BtgI CCRYGG 1 cut(s) 130
Cac8I GCNNGC 1 cut(s) 373
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 5 cut(s) 188, 229, 302, 350, 388
Csp6I GTAC 1 cut(s) 150
CspAI ACCGGT 3 cut(s) 302, 350, 388
CviAII CATG 1 cut(s) 131
CviJI RGCY 5 cut(s) 78, 129, 229, 409, 430
CviKI_1 RGCY 5 cut(s) 78, 129, 229, 409, 430
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 2 cut(s) 8, 295
DpnI GATC 2 cut(s) 62, 335
DpnII GATC 2 cut(s) 60, 333
EaeI YGGCCR 1 cut(s) 428
EagI CGGCCG 1 cut(s) 428
EclXI CGGCCG 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 130
Eco52I CGGCCG 1 cut(s) 428
Eco91I GGTNACC 3 cut(s) 305, 353, 391
EcoO65I GGTNACC 3 cut(s) 305, 353, 391
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 1 cut(s) 134
FaiI YATR 3 cut(s) 23, 116, 132
FatI CATG 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 156, 407, 428
Fsp4HI GCNGC 3 cut(s) 156, 407, 428
GlaI GCGC 1 cut(s) 170
GluI GCNGC 3 cut(s) 156, 407, 428
HaeIII GGCC 2 cut(s) 229, 430
HhaI GCGC 1 cut(s) 171
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 2 cut(s) 399, 420
HphI GGTGA 7 cut(s) 83, 175, 231, 265, 317, 365, 403
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 4 cut(s) 9, 60, 88, 100
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 1 cut(s) 414
Hpy99I CGWCG 3 cut(s) 41, 219, 247
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 219, 243
HpyF3I CTNAG 2 cut(s) 8, 295
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 60, 333
LweI GCATC 1 cut(s) 156
MaeIII GTNAC 7 cut(s) 71, 298, 305, 338, 346, 353, 391
MalI GATC 2 cut(s) 62, 335
MboI GATC 2 cut(s) 60, 333
MboII GAAGA 1 cut(s) 279
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 239, 283
MluCI AATT 2 cut(s) 271, 318
MlyI GAGTC 2 cut(s) 408, 429
MnlI CCTC 6 cut(s) 3, 188, 191, 202, 216, 219
MspR9I CCNGG 2 cut(s) 173, 359
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 173
NciI CCSGG 1 cut(s) 359
NcoI CCATGG 1 cut(s) 130
NdeII GATC 2 cut(s) 60, 333
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 4 cut(s) 71, 305, 353, 391
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 262
PinAI ACCGGT 3 cut(s) 302, 350, 388
PkrI GCNGC 3 cut(s) 157, 408, 429
PleI GAGTC 2 cut(s) 407, 428
PpsI GAGTC 2 cut(s) 407, 428
PshAI GACNNNNGTC 2 cut(s) 398, 419
Psp6I CCWGG 1 cut(s) 171
PspEI GGTNACC 3 cut(s) 305, 353, 391
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 280
PsuI RGATCY 1 cut(s) 60
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
SatI GCNGC 3 cut(s) 156, 407, 428
Sau3AI GATC 2 cut(s) 60, 333
SchI GAGTC 2 cut(s) 408, 429
ScrFI CCNGG 2 cut(s) 173, 359
SduI GDGCHC 2 cut(s) 239, 283
SetI ASST 5 cut(s) 14, 80, 199, 213, 255
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 21
SgrAI CRCCGGYG 1 cut(s) 188
Sse9I AATT 2 cut(s) 271, 318
SsiI CCGC 4 cut(s) 155, 186, 406, 427
StyD4I CCNGG 2 cut(s) 171, 357
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 219, 243
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 271, 318
TauI GCSGC 3 cut(s) 158, 409, 430
TseFI GTSAC 4 cut(s) 71, 305, 353, 391
Tsp45I GTSAC 4 cut(s) 71, 305, 353, 391
XapI RAATTY 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.