FvH4_4g17662

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010516
Physical Location & Seq
Forward (+)
21564336 .. 21567663
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17662.t1

Sequence Viewer

Length: 753 bp
ATGAAGGAGAGATACAACAGCCTAAACCTACCCAGTCTCTTTCGTTTCTCCCACATGGTTGGGCTAGATAGCGGTGAGGGTTGGGTTCAAGGGAGGAGAGGTACTCGTACGACAGCGAGGTCGATCGGCTTTGAAGGAGGCGGCAGAGAGGGTTTTTGTTTAGGGTTTGTTAAGAGTTTCAATTTGTTTTATGATATCAAATTTGGTGGTGATTGGAAGGAGATTTGGTGGTCTGCTCCGACCTCCAGCAGCAACCGCATGCACGCCTCCGACGAACAGAAGAGTAGTGCGGTCGTCAGAGTTGCGTCATTGTCATCCTCTGATCCGTCAACGTCATCGTCGTCCTCGACCCAACCGGATCTGAGACTTCGTCACCGCCTCTCTCTCTCTCTTTGGCTGCCTCGACGTCGACGCCAACGACGCCTCAAGGACGAGGATGACGCACCGGCGAGGAGCCTGACGAGGATCATGGGGTCGAGCCACCTCCGGCATCTCGAGCTACTAGAGGAGGATTGCAACCCCGGCCAGGCTATGGATTTGAAGAGAAGGAGAGAAGGTGAGAAGAGTAGCTGCCATGATTTCATTTTGGATGCAGAGAAATGGTGCAAGGGAGTAGATCCAGTGAGTGAGGGTGCATGCGATGGTGTAGTATACTTTCGGCAAGGTCCGGTGAAAAAATCCGGCGAGATTTCTAACGACCGGCGAGATTCCGGTGACATTTTAAAAAAATCCGGCGAAATTCCGGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

28.19

Weight (kDa)

9.26

Isoelectric Point (pI)

71.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AatII GACGTC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 532
AccI GTMKAC 2 cut(s) 409, 651
AciI CCGC 5 cut(s) 72, 141, 256, 290, 376
AclWI GGATC 4 cut(s) 317, 366, 473, 611
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 2 cut(s) 200, 738
AcyI GRCGYC 3 cut(s) 406, 412, 421
AfaI GTAC 2 cut(s) 103, 109
AfiI CCNNNNNNNGG 2 cut(s) 526, 532
AgsI TTSAA 4 cut(s) 89, 134, 181, 541
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 2 cut(s) 499, 570
AluI AGCT 2 cut(s) 499, 570
Alw26I GTCTC 2 cut(s) 41, 358
AlwI GGATC 4 cut(s) 317, 366, 473, 611
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 3 cut(s) 249, 397, 570
ApoI RAATTY 2 cut(s) 200, 738
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 6 cut(s) 86, 221, 365, 569, 682, 725
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 1 cut(s) 665
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 261, 384, 557
BccI CCATC 1 cut(s) 635
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 2 cut(s) 41, 358
BfaI CTAG 3 cut(s) 65, 503, 751
BisI GCNGC 4 cut(s) 142, 250, 398, 571
BlsI GCNGC 4 cut(s) 143, 251, 399, 572
Bme1390I CCNGG 2 cut(s) 522, 527
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 2 cut(s) 522, 527
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 499, 580
BmuI ACTGGG 1 cut(s) 27
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpmI CTGGAG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 522
BsaHI GRCGYC 3 cut(s) 406, 412, 421
BsaJI CCNNGG 1 cut(s) 520
BsaWI WCCGGW 4 cut(s) 355, 667, 710, 742
BsaXI ACNNNNNCTCC 6 cut(s) 85, 115, 212, 242, 541, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 532
Bse118I RCCGGY 2 cut(s) 445, 699
Bse1I ACTGG 2 cut(s) 33, 620
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 3 cut(s) 314, 442, 595
BseLI CCNNNNNNNGG 2 cut(s) 526, 532
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 2 cut(s) 33, 620
BseRI GAGGAG 3 cut(s) 109, 466, 521
BseXI GCAGC 3 cut(s) 261, 384, 557
Bsh1285I CGRYCG 3 cut(s) 126, 294, 700
BshFI GGCC 1 cut(s) 525
BsiEI CGRYCG 3 cut(s) 126, 294, 700
BsiHKCI CYCGRG 1 cut(s) 494
BsiWI CGTACG 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 526, 532
BsmAI GTCTC 2 cut(s) 41, 358
BsnI GGCC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 5 cut(s) 123, 322, 358, 465, 616
BspACI CCGC 5 cut(s) 72, 141, 256, 290, 376
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 455
BspPI GGATC 4 cut(s) 317, 366, 473, 611
BsrFI RCCGGY 2 cut(s) 445, 699
BsrI ACTGG 2 cut(s) 33, 620
BssAI RCCGGY 2 cut(s) 445, 699
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 5 cut(s) 123, 322, 358, 465, 616
BssNAI GTATAC 1 cut(s) 652
BssNI GRCGYC 3 cut(s) 406, 412, 421
Bst1107I GTATAC 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 527
Bst6I CTCTTC 3 cut(s) 275, 536, 557
BstACI GRCGYC 3 cut(s) 406, 412, 421
BstC8I GCNNGC 3 cut(s) 260, 264, 637
BstDEI CTNAG 1 cut(s) 362
BstF5I GGATG 3 cut(s) 314, 442, 595
BstKTI GATC 5 cut(s) 126, 325, 361, 468, 619
BstMAI GTCTC 2 cut(s) 41, 358
BstMBI GATC 5 cut(s) 123, 322, 358, 465, 616
BstMCI CGRYCG 3 cut(s) 126, 294, 700
BstMWI GCNNNNNNNGC 4 cut(s) 255, 420, 496, 522
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 2 cut(s) 262, 639
BstSCI CCNGG 2 cut(s) 520, 525
BstV1I GCAGC 3 cut(s) 261, 384, 557
BstX2I RGATCY 2 cut(s) 358, 616
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 358, 616
BstZ17I GTATAC 1 cut(s) 652
BsuRI GGCC 1 cut(s) 525
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 3 cut(s) 314, 442, 595
BtsIMutI CAGTG 1 cut(s) 627
Cac8I GCNNGC 3 cut(s) 260, 264, 637
Cfr10I RCCGGY 2 cut(s) 445, 699
Cfr13I GGNCC 1 cut(s) 665
CseI GACGC 4 cut(s) 294, 420, 429, 449
Csp6I GTAC 2 cut(s) 102, 108
CspCI CAANNNNNGTGG 2 cut(s) 187, 222
CviAII CATG 5 cut(s) 55, 259, 469, 575, 636
CviQI GTAC 2 cut(s) 102, 108
DdeI CTNAG 1 cut(s) 362
DpnI GATC 5 cut(s) 125, 324, 360, 467, 618
DpnII GATC 5 cut(s) 123, 322, 358, 465, 616
DraI TTTAAA 1 cut(s) 723
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 3 cut(s) 275, 536, 557
EarI CTCTTC 3 cut(s) 275, 536, 557
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 525
EcoRV GATATC 1 cut(s) 196
FaeI CATG 5 cut(s) 58, 262, 472, 578, 639
FaiI YATR 8 cut(s) 56, 192, 260, 470, 533, 576, 637, 652
FatI CATG 5 cut(s) 54, 258, 468, 574, 635
FblI GTMKAC 2 cut(s) 409, 651
Fnu4HI GCNGC 4 cut(s) 142, 250, 398, 571
FokI GGATG 3 cut(s) 301, 449, 602
Fsp4HI GCNGC 4 cut(s) 142, 250, 398, 571
FspBI CTAG 3 cut(s) 65, 503, 751
GluI GCNGC 4 cut(s) 142, 250, 398, 571
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 1 cut(s) 525
HgaI GACGC 4 cut(s) 294, 420, 429, 449
Hin1I GRCGYC 3 cut(s) 406, 412, 421
Hin1II CATG 5 cut(s) 58, 262, 472, 578, 639
HincII GTYRAC 2 cut(s) 330, 410
HindII GTYRAC 2 cut(s) 330, 410
HinfI GANTC 1 cut(s) 707
HphI GGTGA 6 cut(s) 86, 221, 365, 569, 682, 725
Hpy166II GTNNAC 4 cut(s) 330, 410, 652, 748
Hpy188I TCNGA 5 cut(s) 240, 271, 299, 322, 363
Hpy188III TCNNGA 1 cut(s) 494
Hpy8I GTNNAC 4 cut(s) 330, 410, 652, 748
Hpy99I CGWCG 6 cut(s) 275, 343, 408, 411, 414, 423
HpyAV CCTTC 4 cut(s) 128, 211, 540, 548
HpyCH4IV ACGT 2 cut(s) 332, 406
HpyCH4V TGCA 5 cut(s) 262, 516, 593, 606, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 420, 496, 522
HpyF3I CTNAG 1 cut(s) 362
HpySE526I ACGT 2 cut(s) 332, 406
Hsp92I GRCGYC 3 cut(s) 406, 412, 421
Hsp92II CATG 5 cut(s) 58, 262, 472, 578, 639
Kzo9I GATC 5 cut(s) 123, 322, 358, 465, 616
LmnI GCTCC 2 cut(s) 241, 453
Lsp1109I GCAGC 3 cut(s) 261, 384, 557
LweI GCATC 2 cut(s) 499, 580
MaeI CTAG 3 cut(s) 65, 503, 751
MaeII ACGT 2 cut(s) 332, 406
MaeIII GTNAC 2 cut(s) 371, 713
MalI GATC 5 cut(s) 125, 324, 360, 467, 618
MboI GATC 5 cut(s) 123, 322, 358, 465, 616
MboII GAAGA 3 cut(s) 292, 553, 574
MflI RGATCY 2 cut(s) 358, 616
MluCI AATT 3 cut(s) 181, 200, 738
MmeI TCCRAC 2 cut(s) 263, 294
MseI TTAA 2 cut(s) 171, 722
MspR9I CCNGG 2 cut(s) 522, 527
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 255, 420, 496, 522
NciI CCSGG 1 cut(s) 522
NdeII GATC 5 cut(s) 123, 322, 358, 465, 616
NlaIII CATG 5 cut(s) 58, 262, 472, 578, 639
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 2 cut(s) 371, 713
NspI RCATGY 2 cut(s) 262, 639
PaeI GCATGC 2 cut(s) 262, 639
PaeR7I CTCGAG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 4 cut(s) 270, 338, 344, 415
PfeI GAWTC 1 cut(s) 707
Pfl23II CGTACG 1 cut(s) 107
PflFI GACNNNGTC 1 cut(s) 369
PflMI CCANNNNNTGG 1 cut(s) 532
PkrI GCNGC 4 cut(s) 143, 251, 399, 572
Ple19I CGATCG 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspLI CGTACG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 665
PsuI RGATCY 2 cut(s) 358, 616
PsyI GACNNNGTC 1 cut(s) 369
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 2 cut(s) 103, 109
RsaNI GTAC 2 cut(s) 102, 108
SalI GTCGAC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 171, 722
SatI GCNGC 4 cut(s) 142, 250, 398, 571
Sau3AI GATC 5 cut(s) 123, 322, 358, 465, 616
Sau96I GGNCC 1 cut(s) 665
ScrFI CCNGG 2 cut(s) 522, 527
SfaNI GCATC 2 cut(s) 499, 580
Sfr274I CTCGAG 1 cut(s) 494
SgrAI CRCCGGYG 1 cut(s) 445
SgrDI CGTCGACG 1 cut(s) 408
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 425, 494
SmoI CTYRAG 2 cut(s) 425, 494
SphI GCATGC 2 cut(s) 262, 639
Sse9I AATT 3 cut(s) 181, 200, 738
SsiI CCGC 5 cut(s) 72, 141, 256, 290, 376
SspMI CTAG 3 cut(s) 65, 503, 751
StyD4I CCNGG 2 cut(s) 520, 525
TaiI ACGT 2 cut(s) 335, 409
TaqI TCGA 6 cut(s) 122, 347, 403, 409, 476, 495
TasI AATT 3 cut(s) 181, 200, 738
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 1 cut(s) 707
Tru1I TTAA 2 cut(s) 171, 722
Tru9I TTAA 2 cut(s) 171, 722
TscAI CASTG 1 cut(s) 627
TseFI GTSAC 2 cut(s) 371, 713
TseI GCWGC 3 cut(s) 249, 397, 570
Tsp45I GTSAC 2 cut(s) 371, 713
TspDTI ATGAA 2 cut(s) 17, 571
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 1 cut(s) 627
Tth111I GACNNNGTC 1 cut(s) 369
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 2 cut(s) 200, 738
XceI RCATGY 2 cut(s) 262, 639
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 409, 651
XspI CTAG 3 cut(s) 65, 503, 751
ZraI GACGTC 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.