FvH4_2g40051

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003244
Physical Location & Seq
Forward (+)
28631584 .. 28635491
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40051.t1

Sequence Viewer

Length: 1515 bp
ATGCATCTCCTTTTCCTCCTCCTTCTTTCCACTCTGGTTATCTTCCTCTTCAAATTTGCCTACTCAACCCTATGGGTCCCGTGGAGGACACAAACCCACTTCAGAAAGCAAGGCATAAGGGGCCCCGGCTACCGTCCGATCGTCGGAAACTCCGCCGAGATCCGCCGTCTGTACGCCGAGGCTTCTTCAAAACGAGTCCCATTTGACCACCGCATCGTTCCACGCGTGATTCCATTCTACGACAGGTGGTCCCTCATGTACGGGAAGACTTTCCTCTACTGGTTCGGGCCCAACCCCAGGCTGGCAATATCCGACCCGGATATGATCAAAGAGGTCCTTGTCAATTCCAGTGGGTCGTTTCAGAAGATACCCTTCACCCCTTTAACTAAAAGCCTCTTTGGAGAAGGACTCGGTGGGCTTGAAGGCGATGAATGGGCCTTTCATAGACGGATCGTCAACCAGGCCTTCAAAACGGAGCGGGTAAAGGGTTGGGTGCCTGAAATTGTGGGGAGTGCCATGAAGACGTTGGAGAAGTGGGAGGGTATAAGAGGAGAGAAAGATGAGTTTGAGATGGATGTGCACAAAGAATTTCGTGATCTTTCGGCGGATGTTATATCGAGGACAGCTTTTGGAAGCAGTTTTGAGGAGGGAAAGCGAATTTTCAATTTGCAAGAACAACAAATGCACCTTTTCTCTAAGGCGGTTAGGAGCATCTACATTCCTGGATTCAGGTTTTTGCCCACCAAGAATAACAGAGAAAGGTGGAGACTACAAAAGGAAACTAGTGATTCAATACGGATGTTGCTCTTGAATAGTCCCAAAACAAGAGAAGAATCAACAAATCTACTCAGTCTGTTGATGTCTTCTTACGAAAACCAGAGTGGTGAAGAAGAAAAATTAGGATTAGAGGAAATAATTGCCGAATGCAAGACCTTCTACCTTTCAGGAAGTGAAACAACTGCTTCTCTTTTGACTTGGGCGCTTCTCCTTCTAGCCCGCCATCAGGATTGGCAAAGTAAGGCTAGGGAAGAAGTGATTCGTGCTTGCGGAGACAGTAAGCTTCCTACCGCAGAGAATTTGAACGACTTAAAGATTGTAAGCTTGATAGTTAACGAAACGCTTAGACTCTACCCTCCAGTTGTGATGCTATTGAGGCAAACATCAAAGAAGGTTAAGCTTGGGGACATTGATATTCCTGCTCACACACAACTCTACTTGGCCTTGTCGGCTGTCCATCACGACAATGAGATATGGGGAGACGATGCTAATGAGTTCAATCCCTCGAGATTTTTAGAGCCTCGAAAGCATTTAGCCTCATTCTTTCCATTTGGTCTGGGGCCTAGAATCTGCGCAGGTCAGAACTTATCTCTGGCTGAAGCAAAACTAGTCTTGGCAACCATCATCAGGCACTATTCTTTTGAGGTCTCTCCAACCTATGTCCACGCCCCGATGTTATTGATTAGTTTACAGCCTCAGTATGGTGCTCAAATCCTCTTTAAGAAGATTCCAAGCTGA

Protein Analysis

505

Amino Acids

57.87

Weight (kDa)

9.13

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 86 - 478 7.9e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1351
Acc36I ACCTGC 1 cut(s) 1343
AccB1I GGYRCC 1 cut(s) 493
AccBSI CCGCTC 1 cut(s) 478
AccII CGCG 1 cut(s) 225
AciI CCGC 9 cut(s) 153, 163, 211, 478, 605, 701, 997, 1047, 1068
AclWI GGATC 2 cut(s) 154, 458
AcsI RAATTY 4 cut(s) 53, 587, 657, 1075
AcuI CTGAAG 2 cut(s) 85, 1395
AfaI GTAC 2 cut(s) 173, 260
AfiI CCNNNNNNNGG 6 cut(s) 143, 297, 301, 1003, 1404, 1478
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 9 cut(s) 52, 189, 422, 469, 664, 792, 811, 1081, 1276
AhdI GACNNNNNGTC 2 cut(s) 247, 452
AhlI ACTAGT 2 cut(s) 782, 1384
AjnI CCWGG 3 cut(s) 296, 459, 721
AluBI AGCT 5 cut(s) 626, 1060, 1101, 1177, 1512
AluI AGCT 5 cut(s) 626, 1060, 1101, 1177, 1512
Alw21I GWGCWC 2 cut(s) 582, 1486
Alw26I GTCTC 4 cut(s) 760, 1044, 1251, 1429
Alw44I GTGCAC 1 cut(s) 578
AlwI GGATC 2 cut(s) 154, 458
Ama87I CYCGRG 1 cut(s) 1282
AoxI GGCC 6 cut(s) 121, 287, 435, 462, 1218, 1337
ApaI GGGCCC 2 cut(s) 125, 291
ApaLI GTGCAC 1 cut(s) 578
ApoI RAATTY 4 cut(s) 53, 587, 657, 1075
Asp700I GAANNNNTTC 2 cut(s) 269, 1035
AspLEI GCGC 2 cut(s) 982, 1352
AspS9I GGNCC 9 cut(s) 76, 121, 122, 249, 287, 288, 334, 435, 1337
AsuC2I CCSGG 2 cut(s) 126, 317
AsuHPI GGTGA 2 cut(s) 367, 896
AvaI CYCGRG 1 cut(s) 1282
AvaII GGWCC 3 cut(s) 76, 249, 334
BaeGI GKGCMC 3 cut(s) 125, 291, 582
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 2 cut(s) 125, 291
BbsI GAAGAC 3 cut(s) 272, 527, 855
Bbv12I GWGCWC 2 cut(s) 582, 1486
BccI CCATC 4 cut(s) 565, 1008, 1242, 1406
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 3 cut(s) 298, 461, 723
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 2 cut(s) 126, 317
BcoDI GTCTC 4 cut(s) 760, 1044, 1251, 1429
BcuI ACTAGT 2 cut(s) 782, 1384
BfaI CTAG 5 cut(s) 783, 992, 1023, 1341, 1385
BfoI RGCGCY 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 1343
BglI GCCNNNNNGGC 1 cut(s) 1226
Bme1390I CCNGG 5 cut(s) 126, 298, 317, 461, 723
Bme18I GGWCC 3 cut(s) 76, 249, 334
BmeRI GACNNNNNGTC 2 cut(s) 247, 452
BmeT110I CYCGRG 1 cut(s) 1282
BmgT120I GGNCC 9 cut(s) 76, 121, 122, 249, 287, 288, 334, 435, 1337
BmiI GGNNCC 9 cut(s) 77, 78, 122, 123, 124, 251, 289, 495, 1338
BmrFI CCNGG 5 cut(s) 126, 298, 317, 461, 723
BmsI GCATC 5 cut(s) 13, 222, 720, 1134, 1252
BpiI GAAGAC 3 cut(s) 272, 527, 855
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BpmI CTGGAG 1 cut(s) 1119
BpuMI CCSGG 2 cut(s) 126, 317
BsaI GGTCTC 1 cut(s) 1429
BsaJI CCNNGG 4 cut(s) 80, 124, 177, 296
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 297, 301, 1003, 1404, 1478
Bse1I ACTGG 3 cut(s) 284, 348, 1136
BseBI CCWGG 3 cut(s) 298, 461, 723
BseDI CCNNGG 4 cut(s) 80, 124, 177, 296
BseGI GGATG 3 cut(s) 580, 613, 804
BseLI CCNNNNNNNGG 6 cut(s) 143, 297, 301, 1003, 1404, 1478
BseMII CTCAG 2 cut(s) 862, 1487
BseNI ACTGG 3 cut(s) 284, 348, 1136
BseRI GAGGAG 3 cut(s) 8, 564, 659
BseSI GKGCMC 3 cut(s) 125, 291, 582
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 6 cut(s) 123, 289, 437, 464, 1220, 1339
BshNI GGYRCC 1 cut(s) 493
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 582, 1486
BsiHKCI CYCGRG 1 cut(s) 1282
BsiSI CCGG 2 cut(s) 126, 317
BslFI GGGAC 5 cut(s) 62, 182, 235, 801, 1196
BslI CCNNNNNNNGG 6 cut(s) 143, 297, 301, 1003, 1404, 1478
BsmAI GTCTC 4 cut(s) 760, 1044, 1251, 1429
BsmBI CGTCTC 1 cut(s) 1251
BsmFI GGGAC 5 cut(s) 62, 182, 235, 801, 1196
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 6 cut(s) 123, 289, 437, 464, 1220, 1339
Bso31I GGTCTC 1 cut(s) 1429
BsoBI CYCGRG 1 cut(s) 1282
Bsp120I GGGCCC 2 cut(s) 121, 287
Bsp1286I GDGCHC 4 cut(s) 125, 291, 582, 1486
Bsp143I GATC 5 cut(s) 138, 159, 324, 450, 595
BspACI CCGC 9 cut(s) 153, 163, 211, 478, 605, 701, 997, 1047, 1068
BspANI GGCC 6 cut(s) 123, 289, 437, 464, 1220, 1339
BspCNI CTCAG 2 cut(s) 861, 1486
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 9 cut(s) 77, 78, 122, 123, 124, 251, 289, 495, 1338
BspMI ACCTGC 1 cut(s) 1343
BspPI GGATC 2 cut(s) 154, 458
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 1429
BsrBI CCGCTC 1 cut(s) 478
BsrI ACTGG 3 cut(s) 284, 348, 1136
BssECI CCNNGG 4 cut(s) 80, 124, 177, 296
BssMI GATC 5 cut(s) 138, 159, 324, 450, 595
Bst2UI CCWGG 3 cut(s) 298, 461, 723
Bst4CI ACNGT 2 cut(s) 134, 1055
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 303, 997, 1045
BstDEI CTNAG 4 cut(s) 696, 848, 1121, 1473
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 580, 613, 804
BstFNI CGCG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 2 cut(s) 982, 1352
BstKTI GATC 5 cut(s) 141, 162, 327, 453, 598
BstMAI GTCTC 4 cut(s) 760, 1044, 1251, 1429
BstMBI GATC 5 cut(s) 138, 159, 324, 450, 595
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 120, 1153, 1226, 1303
BstNI CCWGG 3 cut(s) 298, 461, 723
BstSCI CCNGG 5 cut(s) 124, 296, 315, 459, 721
BstSLI GKGCMC 3 cut(s) 125, 291, 582
BstUI CGCG 1 cut(s) 225
BstV2I GAAGAC 3 cut(s) 272, 527, 855
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 6 cut(s) 123, 289, 437, 464, 1220, 1339
BtgI CCRYGG 1 cut(s) 80
BtgZI GCGATG 1 cut(s) 441
BtsCI GGATG 3 cut(s) 580, 613, 804
BtsIMutI CAGTG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 1343
Cac8I GCNNGC 3 cut(s) 303, 997, 1045
CfoI GCGC 2 cut(s) 982, 1352
Cfr13I GGNCC 9 cut(s) 76, 121, 122, 249, 287, 288, 334, 435, 1337
Csp6I GTAC 2 cut(s) 172, 259
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 2 cut(s) 256, 517
CviQI GTAC 2 cut(s) 172, 259
DdeI CTNAG 4 cut(s) 696, 848, 1121, 1473
DpnI GATC 5 cut(s) 140, 161, 326, 452, 597
DpnII GATC 5 cut(s) 138, 159, 324, 450, 595
DriI GACNNNNNGTC 2 cut(s) 247, 452
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 2 cut(s) 247, 452
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 3 cut(s) 142, 152, 620
Eco147I AGGCCT 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 125, 291
Eco31I GGTCTC 1 cut(s) 1429
Eco47I GGWCC 3 cut(s) 76, 249, 334
Eco57I CTGAAG 2 cut(s) 85, 1395
Eco88I CYCGRG 1 cut(s) 1282
EcoO109I RGGNCCY 5 cut(s) 76, 121, 122, 334, 1337
EcoRII CCWGG 3 cut(s) 296, 459, 721
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 125, 291
Esp3I CGTCTC 1 cut(s) 1251
FaeI CATG 2 cut(s) 259, 520
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 356, 388
FaqI GGGAC 5 cut(s) 62, 182, 235, 801, 1196
FatI CATG 2 cut(s) 255, 516
FauI CCCGC 2 cut(s) 471, 1004
FbaI TGATCA 1 cut(s) 324
FokI GGATG 3 cut(s) 587, 620, 811
FriOI GRGCYC 2 cut(s) 125, 291
FspBI CTAG 5 cut(s) 783, 992, 1023, 1341, 1385
FspI TGCGCA 1 cut(s) 1351
GlaI GCGC 2 cut(s) 981, 1351
GsuI CTGGAG 1 cut(s) 1119
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 6 cut(s) 123, 289, 437, 464, 1220, 1339
HapII CCGG 2 cut(s) 126, 317
HhaI GCGC 2 cut(s) 982, 1352
Hin1II CATG 2 cut(s) 259, 520
Hin6I GCGC 2 cut(s) 980, 1350
HinP1I GCGC 2 cut(s) 980, 1350
HincII GTYRAC 2 cut(s) 457, 1111
HindII GTYRAC 2 cut(s) 457, 1111
HindIII AAGCTT 3 cut(s) 1058, 1099, 1175
HpaI GTTAAC 1 cut(s) 1111
HpaII CCGG 2 cut(s) 126, 317
HphI GGTGA 2 cut(s) 367, 896
Hpy166II GTNNAC 5 cut(s) 457, 580, 1111, 1441, 1466
Hpy188I TCNGA 6 cut(s) 104, 138, 146, 313, 363, 1359
Hpy188III TCNNGA 6 cut(s) 593, 808, 945, 1004, 1238, 1284
Hpy8I GTNNAC 5 cut(s) 457, 580, 1111, 1441, 1466
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 8 cut(s) 32, 382, 398, 416, 475, 943, 998, 1162
HpyCH4III ACNGT 2 cut(s) 134, 1055
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 5 cut(s) 4, 580, 670, 685, 927
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 1153, 1226, 1303
HpyF3I CTNAG 4 cut(s) 696, 848, 1121, 1473
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 2 cut(s) 259, 520
HspAI GCGC 2 cut(s) 980, 1350
KflI GGGWCCC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 1111
Kzo9I GATC 5 cut(s) 138, 159, 324, 450, 595
LmnI GCTCC 2 cut(s) 475, 708
LweI GCATC 5 cut(s) 13, 222, 720, 1134, 1252
MaeI CTAG 5 cut(s) 783, 992, 1023, 1341, 1385
MaeII ACGT 1 cut(s) 524
MalI GATC 5 cut(s) 140, 161, 326, 452, 597
MbiI CCGCTC 1 cut(s) 478
MboI GATC 5 cut(s) 138, 159, 324, 450, 595
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 4 cut(s) 125, 291, 582, 1486
MluCI AATT 9 cut(s) 53, 343, 501, 587, 657, 664, 896, 915, 1075
MluI ACGCGT 1 cut(s) 223
MlyI GAGTC 3 cut(s) 204, 402, 1119
MmeI TCCRAC 4 cut(s) 124, 336, 507, 1454
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 269, 1035
MseI TTAA 5 cut(s) 383, 1088, 1110, 1173, 1497
MslI CAYNNNNRTG 1 cut(s) 1242
MspI CCGG 2 cut(s) 126, 317
MspR9I CCNGG 5 cut(s) 126, 298, 317, 461, 723
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 3 cut(s) 298, 461, 723
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 120, 1153, 1226, 1303
NciI CCSGG 2 cut(s) 126, 317
NdeII GATC 5 cut(s) 138, 159, 324, 450, 595
NlaIII CATG 2 cut(s) 259, 520
NlaIV GGNNCC 9 cut(s) 77, 78, 122, 123, 124, 251, 289, 495, 1338
NmeAIII GCCGAG 2 cut(s) 181, 202
NsbI TGCGCA 1 cut(s) 1351
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 1282
PceI AGGCCT 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 929
PdmI GAANNNNTTC 2 cut(s) 269, 1035
PfeI GAWTC 7 cut(s) 229, 726, 788, 833, 1036, 1344, 1504
PfoI TCCNGGA 1 cut(s) 721
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 3 cut(s) 203, 402, 1119
PpsI GAGTC 3 cut(s) 203, 402, 1119
PpuMI RGGWCCY 2 cut(s) 76, 334
Psp5II RGGWCCY 2 cut(s) 76, 334
Psp6I CCWGG 3 cut(s) 296, 459, 721
PspGI CCWGG 3 cut(s) 296, 459, 721
PspN4I GGNNCC 9 cut(s) 77, 78, 122, 123, 124, 251, 289, 495, 1338
PspOMI GGGCCC 2 cut(s) 121, 287
PspPI GGNCC 9 cut(s) 76, 121, 122, 249, 287, 288, 334, 435, 1337
PspPPI RGGWCCY 2 cut(s) 76, 334
PsuI RGATCY 1 cut(s) 159
PvuI CGATCG 1 cut(s) 141
RsaI GTAC 2 cut(s) 173, 260
RsaNI GTAC 2 cut(s) 172, 259
RseI CAYNNNNRTG 1 cut(s) 1242
SaqAI TTAA 5 cut(s) 383, 1088, 1110, 1173, 1497
Sau3AI GATC 5 cut(s) 138, 159, 324, 450, 595
Sau96I GGNCC 9 cut(s) 76, 121, 122, 249, 287, 288, 334, 435, 1337
SchI GAGTC 3 cut(s) 204, 402, 1119
ScrFI CCNGG 5 cut(s) 126, 298, 317, 461, 723
SduI GDGCHC 4 cut(s) 125, 291, 582, 1486
SfaNI GCATC 5 cut(s) 13, 222, 720, 1134, 1252
Sfr274I CTCGAG 1 cut(s) 1282
SinI GGWCC 3 cut(s) 76, 249, 334
SlaI CTCGAG 1 cut(s) 1282
SmiMI CAYNNNNRTG 1 cut(s) 1242
SmlI CTYRAG 1 cut(s) 1282
SmoI CTYRAG 1 cut(s) 1282
SpeI ACTAGT 2 cut(s) 782, 1384
Sse9I AATT 9 cut(s) 53, 343, 501, 587, 657, 664, 896, 915, 1075
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 9 cut(s) 153, 163, 211, 478, 605, 701, 997, 1047, 1068
SspMI CTAG 5 cut(s) 783, 992, 1023, 1341, 1385
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 5 cut(s) 124, 296, 315, 459, 721
TaaI ACNGT 2 cut(s) 134, 1055
TaiI ACGT 1 cut(s) 527
TaqI TCGA 3 cut(s) 617, 1283, 1300
TasI AATT 9 cut(s) 53, 343, 501, 587, 657, 664, 896, 915, 1075
TfiI GAWTC 7 cut(s) 229, 726, 788, 833, 1036, 1344, 1504
Tru1I TTAA 5 cut(s) 383, 1088, 1110, 1173, 1497
Tru9I TTAA 5 cut(s) 383, 1088, 1110, 1173, 1497
TscAI CASTG 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 431, 444, 533
TspGWI ACGGA 3 cut(s) 463, 488, 811
TspRI CASTG 1 cut(s) 355
VneI GTGCAC 1 cut(s) 578
VpaK11BI GGWCC 3 cut(s) 76, 249, 334
XapI RAATTY 4 cut(s) 53, 587, 657, 1075
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 523
XhoI CTCGAG 1 cut(s) 1282
XmnI GAANNNNTTC 2 cut(s) 269, 1035
XspI CTAG 5 cut(s) 783, 992, 1023, 1341, 1385
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.