RchiOBHm_Chr6g0300701

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003244
Physical Location & Seq
Reverse (-)
61455098 .. 61456875
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27005

Sequence Viewer

Length: 765 bp
ATGCATCTTCTTCTGCTTTTTGTTCTTCCTCTTATTTTCATCTTGAAATTTGTCTACTCCACAATATTGGTTCCGTGGAGGACACGAAACCACTTCCTCAAGCAAGGCATTGATGGACCTGACTATCGTCCGATCATCGGCAACTCGGCCGAGATCCGCCTTATGTTCAAAGAAGCTCTGTCAAAGCGAGTCCCTTTTGACCACAACGTACTCGGACGCACGCTTCCATTTTACCCTAGCTGGTCCAAAGTGTATGGGAAGAACTTCTTGTACTGGTTCGGGTCAAAGCCCAGGCTGGCTATAGCCGACCCGGTAATGATCAAACAAGTCCTTATCAATACCAGTGGATCGTTTCAAAGAGTACCTTTAACTCCTTCCTCTAGGGTTCTCTTTGGAGATGGACTTCCTGCGCTCGAAGGTGAAAGATGGGCTTTCCATAGAAAAATTGTAAACCAAGCCTTCAAAATGGAGCGAGTCGAGGATTGGGTGCCACAAATTGTGGACATTACCATGAAGATGATGGAGGAGTGGGAGGATATAAGGGGAGGAAGAGATGAGTTTGAAATGGAGGTACACAAACAAATGCATGAGGTATCAGCAGATGTTATGTCCAGAACAGTTTTCGGAAGCAGTTTTGAAGAGGGAAAGCGTATTTTCAACTTGCAAAAACAGCAAATGCATCTTTTCCTTAAGGCAGTTCAGAGCATCTATATTCCTGGATTCAGATTTTTGCCTACCAAGGATACAGAGAGAGGTGGAGACTAA

Protein Analysis

254

Amino Acids

29.66

Weight (kDa)

9.46

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 82 - 247 3e-15 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 54
AciI CCGC 1 cut(s) 157
AclWI GGATC 2 cut(s) 148, 355
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 4 cut(s) 210, 272, 363, 573
AfiI CCNNNNNNNGG 1 cut(s) 137
AflII CTTAAG 1 cut(s) 689
AgsI TTSAA 7 cut(s) 46, 169, 356, 463, 563, 638, 658
AjnI CCWGG 2 cut(s) 290, 715
AluBI AGCT 2 cut(s) 176, 240
AluI AGCT 2 cut(s) 176, 240
Alw26I GTCTC 1 cut(s) 753
AlwI GGATC 2 cut(s) 148, 355
AoxI GGCC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 2 cut(s) 116, 243
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 2 cut(s) 116, 243
BanI GGYRCC 1 cut(s) 487
BccI CCATC 4 cut(s) 107, 392, 420, 514
BciT130I CCWGG 2 cut(s) 292, 717
BciVI GTATCC 1 cut(s) 736
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 753
BfaI CTAG 2 cut(s) 237, 381
BfmI CTRYAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 689
BfuI GTATCC 1 cut(s) 736
Bme1390I CCNGG 3 cut(s) 292, 311, 717
Bme18I GGWCC 2 cut(s) 116, 243
BmgT120I GGNCC 2 cut(s) 116, 243
BmiI GGNNCC 2 cut(s) 72, 489
BmrFI CCNGG 3 cut(s) 292, 311, 717
BmsI GCATC 3 cut(s) 13, 688, 714
BoxI GACNNNNGTC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 74, 290, 738
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 278, 342
BseBI CCWGG 2 cut(s) 292, 717
BseDI CCNNGG 3 cut(s) 74, 290, 738
BseLI CCNNNNNNNGG 1 cut(s) 137
BseNI ACTGG 2 cut(s) 278, 342
BseRI GAGGAG 1 cut(s) 539
BseX3I CGGCCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 149
BshNI GGYRCC 1 cut(s) 487
BsiEI CGRYCG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 753
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 1 cut(s) 149
Bsp143I GATC 4 cut(s) 132, 153, 318, 347
BspACI CCGC 1 cut(s) 157
BspANI GGCC 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 72, 489
BspPI GGATC 2 cut(s) 148, 355
BspT107I GGYRCC 1 cut(s) 487
BspTI CTTAAG 1 cut(s) 689
BsrI ACTGG 2 cut(s) 278, 342
BssECI CCNNGG 3 cut(s) 74, 290, 738
BssMI GATC 4 cut(s) 132, 153, 318, 347
BssT1I CCWWGG 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 292, 717
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 2 cut(s) 544, 633
BstAFI CTTAAG 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 221, 297
BstDSI CCRYGG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 4 cut(s) 135, 156, 321, 350
BstMAI GTCTC 1 cut(s) 753
BstMBI GATC 4 cut(s) 132, 153, 318, 347
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 670
BstNI CCWGG 2 cut(s) 292, 717
BstPAI GACNNNNGTC 1 cut(s) 126
BstSCI CCNGG 3 cut(s) 290, 309, 715
BstSFI CTRYAG 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 153
BstZI CGGCCG 1 cut(s) 147
BsuI GTATCC 1 cut(s) 736
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 74
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 2 cut(s) 221, 297
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 116, 243
CseI GACGC 1 cut(s) 225
Csp6I GTAC 4 cut(s) 209, 271, 362, 572
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 2 cut(s) 511, 587
CviJI RGCY 9 cut(s) 149, 176, 240, 289, 295, 299, 305, 431, 458
CviKI_1 RGCY 9 cut(s) 149, 176, 240, 289, 295, 299, 305, 431, 458
CviQI GTAC 4 cut(s) 209, 271, 362, 572
DpnI GATC 4 cut(s) 134, 155, 320, 349
DpnII GATC 4 cut(s) 132, 153, 318, 347
EaeI YGGCCR 1 cut(s) 147
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 544, 633
EarI CTCTTC 2 cut(s) 544, 633
EciI GGCGGA 1 cut(s) 146
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 738
Eco47I GGWCC 2 cut(s) 116, 243
Eco52I CGGCCG 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 290, 715
EcoT14I CCWWGG 1 cut(s) 738
EcoT22I ATGCAT 3 cut(s) 6, 588, 681
ErhI CCWWGG 1 cut(s) 738
FaeI CATG 2 cut(s) 514, 590
FaiI YATR 9 cut(s) 164, 255, 302, 438, 512, 539, 588, 608, 711
FalI AAGNNNNNCTT 6 cut(s) 251, 283, 349, 381, 415, 447
FaqI GGGAC 1 cut(s) 176
FatI CATG 2 cut(s) 510, 586
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 1 cut(s) 54
FspBI CTAG 2 cut(s) 237, 381
GlaI GCGC 1 cut(s) 411
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 225
HhaI GCGC 1 cut(s) 412
Hin1II CATG 2 cut(s) 514, 590
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HinfI GANTC 3 cut(s) 189, 474, 720
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 4 cut(s) 55, 451, 502, 574
Hpy188I TCNGA 5 cut(s) 132, 215, 626, 702, 725
Hpy188III TCNNGA 2 cut(s) 43, 612
Hpy8I GTNNAC 4 cut(s) 55, 451, 502, 574
HpyAV CCTTC 3 cut(s) 384, 410, 469
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 4 cut(s) 4, 586, 664, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 670
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 2 cut(s) 514, 590
HspAI GCGC 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 4 cut(s) 132, 153, 318, 347
LmnI GCTCC 1 cut(s) 469
LweI GCATC 3 cut(s) 13, 688, 714
MaeI CTAG 2 cut(s) 237, 381
MaeII ACGT 1 cut(s) 207
MalI GATC 4 cut(s) 134, 155, 320, 349
MboI GATC 4 cut(s) 132, 153, 318, 347
MboII GAAGA 5 cut(s) 17, 271, 526, 561, 650
MflI RGATCY 1 cut(s) 153
MluCI AATT 3 cut(s) 47, 444, 495
MlyI GAGTC 2 cut(s) 198, 483
Mph1103I ATGCAT 3 cut(s) 6, 588, 681
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 368, 690
MslI CAYNNNNRTG 2 cut(s) 509, 515
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 3 cut(s) 292, 311, 717
MvaI CCWGG 2 cut(s) 292, 717
MwoI GCNNNNNNNGC 1 cut(s) 670
NciI CCSGG 1 cut(s) 311
NdeII GATC 4 cut(s) 132, 153, 318, 347
NlaIII CATG 2 cut(s) 514, 590
NlaIV GGNNCC 2 cut(s) 72, 489
NmeAIII GCCGAG 2 cut(s) 125, 175
NsiI ATGCAT 3 cut(s) 6, 588, 681
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 715
PleI GAGTC 2 cut(s) 197, 482
PpsI GAGTC 2 cut(s) 197, 482
PshAI GACNNNNGTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 290, 715
PspGI CCWGG 2 cut(s) 290, 715
PspN4I GGNNCC 2 cut(s) 72, 489
PspPI GGNCC 2 cut(s) 116, 243
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 4 cut(s) 210, 272, 363, 573
RsaNI GTAC 4 cut(s) 209, 271, 362, 572
RseI CAYNNNNRTG 2 cut(s) 509, 515
SaqAI TTAA 2 cut(s) 368, 690
Sau3AI GATC 4 cut(s) 132, 153, 318, 347
Sau96I GGNCC 2 cut(s) 116, 243
SchI GAGTC 2 cut(s) 198, 483
ScrFI CCNGG 3 cut(s) 292, 311, 717
SetI ASST 9 cut(s) 121, 178, 210, 242, 367, 421, 573, 594, 757
SfaNI GCATC 3 cut(s) 13, 688, 714
SfcI CTRYAG 1 cut(s) 300
SinI GGWCC 2 cut(s) 116, 243
SmiMI CAYNNNNRTG 2 cut(s) 509, 515
SmlI CTYRAG 2 cut(s) 98, 689
SmoI CTYRAG 2 cut(s) 98, 689
Sse9I AATT 3 cut(s) 47, 444, 495
SsiI CCGC 1 cut(s) 157
SspI AATATT 1 cut(s) 66
SspMI CTAG 2 cut(s) 237, 381
StyD4I CCNGG 3 cut(s) 290, 309, 715
StyI CCWWGG 1 cut(s) 738
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 414, 477
TasI AATT 3 cut(s) 47, 444, 495
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 1 cut(s) 720
Tru1I TTAA 2 cut(s) 368, 690
Tru9I TTAA 2 cut(s) 368, 690
TscAI CASTG 1 cut(s) 349
TspDTI ATGAA 2 cut(s) 28, 527
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 2 cut(s) 116, 243
XapI RAATTY 1 cut(s) 47
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 237, 381
Zsp2I ATGCAT 3 cut(s) 6, 588, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.