Rw6G035980

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003244
Physical Location & Seq
Reverse (-)
60564664 .. 60566563
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035980.1

Sequence Viewer

Length: 1203 bp
ATGATCAAACAAGTCCTTATCAATACCAGTGGATCGTTTCAAAGAGTACCCTTAACTCCTTCCTCTAGGGTTCTCTTTGGAGATGGACTTCCTGCGCTCGAAGGTGAAAGATGGGCTTTCCATAGAAAAATTGTCAACCAAGCCTTCAAAATGGAGCGAGTCGAGGATTGGGTGCCACAAATTGTGGACATTACCATGAAGATGATGGAGGAGTGGGAGCATATAAGGGGAGGAAGAGATGAGTTTGAAATGGAGGTACACAAACAAATGCATGAGGTATCAGCAGACGTTATGTCCAGAACAGTTTTCGGAAGCAGTTTTGAAGAGGGAAAGCGTATTTTCAACTTGCAAAAACAGCAAATGCATCTTTTCCTTAAGGCAGTTCAGAGCATCTATATTCCTGGATTCAGATTTTTGCCTACCAAGGATAACAGAGAGAGGTGGAGACTAAATAAGGAAATTTGTGAATCAATACGGATGTTGATTGCGAATAATAGCAAAAACCTAGAGGAATCAAAAGTTCTGCTGAGTCTATTAATGTCTTCTCACAAGAATCTGGATGGAATAGAGGAAAAACTAGGGCTACAGGAAATTATAGAAGAATGCAAGGCCTTCTACTTTGTTGGCAAGGAAACAATTGCTAATCTTTTGACTTGGTCACTTATCCTTCTGGCGCGGCATCAGGAATGGCAAAGCAAGGCTAGAGAAGAAGCGATTATTTTCCTTTGTGGTCCTTGTGGACACAATAAGCATCCAGTCGAAGAGAATTTGAACGACCTCAGGATTGTAAGCATGATAATTAACGAAGCACTTAGACTCTATCCTCCTGGTGTGCTACTAAATAGGCAAACATCAAAGAAGCTTAAGCTTGGGAGCCTGGAAATTCCTGCTGACACACAACTAGTCGTGCCCTTGATTGCTGTTCATCACGACACTGATATATGGGGTAAAGATGCTAATGAATTCAATCCATCAAGGTTCTTGGAGCCTCGAAGGCATTTAGCCTCGTTTCTTCCATTTGGTTTAGGGACCAGATACTGCCCCGGTCAGAATTTAGCACTCGTTGAAGCAAAACTGGTGTTAGCAATCATCGTCAGACACTATTCTTTCACGGTGTCAGCTACATATGTCCATGCCCCAAAGTTGTATGTAAACTTAGAGCCCCAATTTGGCGCTCAACTCCTATTTACAAGGATTCCCTAA

Protein Analysis

400

Amino Acids

46.08

Weight (kDa)

8.19

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 372 4.8e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 172
AccII CGCG 1 cut(s) 676
AciI CCGC 1 cut(s) 676
AclWI GGATC 1 cut(s) 40
AcsI RAATTY 5 cut(s) 459, 766, 882, 962, 1051
AfaI GTAC 2 cut(s) 48, 258
AfiI CCNNNNNNNGG 1 cut(s) 1169
AflII CTTAAG 2 cut(s) 374, 863
AgsI TTSAA 8 cut(s) 41, 148, 248, 323, 343, 772, 967, 1067
AhdI GACNNNNNGTC 1 cut(s) 292
AhlI ACTAGT 1 cut(s) 901
AjnI CCWGG 3 cut(s) 400, 826, 876
AluBI AGCT 3 cut(s) 862, 868, 1121
AluI AGCT 3 cut(s) 862, 868, 1121
Alw26I GTCTC 1 cut(s) 439
AlwI GGATC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 1038
AoxI GGCC 1 cut(s) 609
ApoI RAATTY 5 cut(s) 459, 766, 882, 962, 1051
AseI ATTAAT 1 cut(s) 536
AspLEI GCGC 3 cut(s) 97, 676, 1175
AspS9I GGNCC 2 cut(s) 731, 1029
AsuC2I CCSGG 1 cut(s) 1044
AsuHPI GGTGA 1 cut(s) 116
AvaII GGWCC 2 cut(s) 731, 1029
AxyI CCTNAGG 1 cut(s) 779
BaeGI GKGCMC 1 cut(s) 912
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 1164
BbsI GAAGAC 1 cut(s) 534
BccI CCATC 5 cut(s) 77, 105, 199, 554, 979
BciT130I CCWGG 3 cut(s) 402, 828, 878
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1044
BcoDI GTCTC 1 cut(s) 439
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 5 cut(s) 66, 506, 578, 702, 902
BfmI CTRYAG 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 1176
BfrI CTTAAG 2 cut(s) 374, 863
BglI GCCNNNNNGGC 1 cut(s) 994
BisI GCNGC 1 cut(s) 677
BlsI GCNGC 1 cut(s) 678
Bme1390I CCNGG 4 cut(s) 402, 828, 878, 1044
Bme18I GGWCC 2 cut(s) 731, 1029
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 731, 1029
BmiI GGNNCC 4 cut(s) 174, 875, 987, 1030
BmrFI CCNGG 4 cut(s) 402, 828, 878, 1044
BmsI GCATC 5 cut(s) 373, 399, 688, 760, 943
BpiI GAAGAC 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 1044
BsaJI CCNNGG 2 cut(s) 423, 1042
Bsc4I CCNNNNNNNGG 1 cut(s) 1169
Bse1I ACTGG 3 cut(s) 27, 755, 1080
Bse21I CCTNAGG 1 cut(s) 779
BseBI CCWGG 3 cut(s) 402, 828, 878
BseDI CCNNGG 2 cut(s) 423, 1042
BseGI GGATG 3 cut(s) 483, 565, 751
BseLI CCNNNNNNNGG 1 cut(s) 1169
BseMII CTCAG 2 cut(s) 518, 793
BseNI ACTGG 3 cut(s) 27, 755, 1080
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 912
Bsh1236I CGCG 1 cut(s) 676
BshFI GGCC 1 cut(s) 611
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 1044
BslFI GGGAC 1 cut(s) 1042
BslI CCNNNNNNNGG 1 cut(s) 1169
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 1042
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 912, 1164
Bsp143I GATC 2 cut(s) 3, 32
BspACI CCGC 1 cut(s) 676
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 2 cut(s) 519, 792
BspFNI CGCG 1 cut(s) 676
BspLI GGNNCC 4 cut(s) 174, 875, 987, 1030
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 172
BspTI CTTAAG 2 cut(s) 374, 863
BsrI ACTGG 3 cut(s) 27, 755, 1080
BssECI CCNNGG 2 cut(s) 423, 1042
BssMI GATC 2 cut(s) 3, 32
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 3 cut(s) 402, 828, 878
Bst4CI ACNGT 2 cut(s) 304, 1114
Bst6I CTCTTC 3 cut(s) 229, 318, 756
BstAFI CTTAAG 2 cut(s) 374, 863
BstDEI CTNAG 4 cut(s) 527, 779, 812, 1156
BstF5I GGATG 3 cut(s) 483, 565, 751
BstFNI CGCG 1 cut(s) 676
BstH2I RGCGCY 1 cut(s) 1176
BstHHI GCGC 3 cut(s) 97, 676, 1175
BstKTI GATC 2 cut(s) 6, 35
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 2 cut(s) 3, 32
BstMWI GCNNNNNNNGC 2 cut(s) 355, 994
BstNI CCWGG 3 cut(s) 402, 828, 878
BstSCI CCNGG 4 cut(s) 400, 826, 876, 1042
BstSFI CTRYAG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 912
BstUI CGCG 1 cut(s) 676
BstV2I GAAGAC 1 cut(s) 534
Bsu36I CCTNAGG 1 cut(s) 779
BsuRI GGCC 1 cut(s) 611
BtsCI GGATG 3 cut(s) 483, 565, 751
BtsIMutI CAGTG 2 cut(s) 34, 933
CaiI CAGNNNCTG 1 cut(s) 1038
CfoI GCGC 3 cut(s) 97, 676, 1175
Cfr13I GGNCC 2 cut(s) 731, 1029
Csp6I GTAC 2 cut(s) 47, 257
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 4 cut(s) 196, 272, 793, 1133
CviQI GTAC 2 cut(s) 47, 257
DdeI CTNAG 4 cut(s) 527, 779, 812, 1156
DpnI GATC 2 cut(s) 5, 34
DpnII GATC 2 cut(s) 3, 32
DriI GACNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 3 cut(s) 229, 318, 756
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 3 cut(s) 229, 318, 756
Eco130I CCWWGG 1 cut(s) 423
Eco147I AGGCCT 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 1164
Eco47I GGWCC 2 cut(s) 731, 1029
Eco81I CCTNAGG 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 3 cut(s) 400, 826, 876
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 2 cut(s) 273, 366
EcoT38I GRGCYC 1 cut(s) 1164
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 4 cut(s) 199, 275, 796, 1136
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 1 cut(s) 1042
FatI CATG 4 cut(s) 195, 271, 792, 1132
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 677
FokI GGATG 3 cut(s) 490, 572, 738
FriOI GRGCYC 1 cut(s) 1164
Fsp4HI GCNGC 1 cut(s) 677
FspBI CTAG 5 cut(s) 66, 506, 578, 702, 902
GlaI GCGC 3 cut(s) 96, 675, 1174
GluI GCNGC 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 1176
HaeIII GGCC 1 cut(s) 611
HapII CCGG 1 cut(s) 1044
HhaI GCGC 3 cut(s) 97, 676, 1175
Hin1II CATG 4 cut(s) 199, 275, 796, 1136
Hin6I GCGC 3 cut(s) 95, 674, 1173
HinP1I GCGC 3 cut(s) 95, 674, 1173
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 860, 866
HinfI GANTC 8 cut(s) 159, 405, 467, 512, 529, 553, 816, 1195
HpaII CCGG 1 cut(s) 1044
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 5 cut(s) 136, 187, 259, 740, 1153
Hpy188I TCNGA 5 cut(s) 311, 387, 410, 1050, 1097
Hpy188III TCNNGA 5 cut(s) 297, 557, 683, 781, 929
Hpy8I GTNNAC 5 cut(s) 136, 187, 259, 740, 1153
HpyAV CCTTC 6 cut(s) 69, 95, 154, 622, 677, 987
HpyCH4III ACNGT 2 cut(s) 304, 1114
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 4 cut(s) 271, 349, 364, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 994
HpyF3I CTNAG 4 cut(s) 527, 779, 812, 1156
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 4 cut(s) 199, 275, 796, 1136
HspAI GCGC 3 cut(s) 95, 674, 1173
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 32
LmnI GCTCC 4 cut(s) 154, 217, 873, 985
LweI GCATC 5 cut(s) 373, 399, 688, 760, 943
MaeI CTAG 5 cut(s) 66, 506, 578, 702, 902
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 657
MalI GATC 2 cut(s) 5, 34
MboI GATC 2 cut(s) 3, 32
MboII GAAGA 8 cut(s) 211, 246, 335, 534, 611, 719, 773, 1004
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 2 cut(s) 912, 1164
MlyI GAGTC 3 cut(s) 168, 538, 810
Mph1103I ATGCAT 2 cut(s) 273, 366
MseI TTAA 5 cut(s) 53, 375, 536, 801, 864
MslI CAYNNNNRTG 2 cut(s) 194, 200
MspCI CTTAAG 2 cut(s) 374, 863
MspI CCGG 1 cut(s) 1044
MspR9I CCNGG 4 cut(s) 402, 828, 878, 1044
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 3 cut(s) 402, 828, 878
MvnI CGCG 1 cut(s) 676
MwoI GCNNNNNNNGC 2 cut(s) 355, 994
NciI CCSGG 1 cut(s) 1044
NdeI CATATG 1 cut(s) 1126
NdeII GATC 2 cut(s) 3, 32
NlaIII CATG 4 cut(s) 199, 275, 796, 1136
NlaIV GGNNCC 4 cut(s) 174, 875, 987, 1030
NmuCI GTSAC 1 cut(s) 657
NsiI ATGCAT 2 cut(s) 273, 366
PceI AGGCCT 1 cut(s) 611
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 5 cut(s) 405, 467, 512, 553, 1195
PflFI GACNNNGTC 1 cut(s) 655
PfoI TCCNGGA 1 cut(s) 400
PkrI GCNGC 1 cut(s) 678
PleI GAGTC 3 cut(s) 167, 537, 810
PpsI GAGTC 3 cut(s) 167, 537, 810
PshBI ATTAAT 1 cut(s) 536
Psp6I CCWGG 3 cut(s) 400, 826, 876
PspGI CCWGG 3 cut(s) 400, 826, 876
PspN4I GGNNCC 4 cut(s) 174, 875, 987, 1030
PspPI GGNCC 2 cut(s) 731, 1029
PstNI CAGNNNCTG 1 cut(s) 1038
PsyI GACNNNGTC 1 cut(s) 655
RsaI GTAC 2 cut(s) 48, 258
RsaNI GTAC 2 cut(s) 47, 257
RseI CAYNNNNRTG 2 cut(s) 194, 200
SaqAI TTAA 5 cut(s) 53, 375, 536, 801, 864
SatI GCNGC 1 cut(s) 677
Sau3AI GATC 2 cut(s) 3, 32
Sau96I GGNCC 2 cut(s) 731, 1029
SchI GAGTC 3 cut(s) 168, 538, 810
ScrFI CCNGG 4 cut(s) 402, 828, 878, 1044
SduI GDGCHC 2 cut(s) 912, 1164
SfaNI GCATC 5 cut(s) 373, 399, 688, 760, 943
SfcI CTRYAG 1 cut(s) 584
SinI GGWCC 2 cut(s) 731, 1029
SmiMI CAYNNNNRTG 2 cut(s) 194, 200
SmlI CTYRAG 2 cut(s) 374, 863
SmoI CTYRAG 2 cut(s) 374, 863
SpeI ACTAGT 1 cut(s) 901
SseBI AGGCCT 1 cut(s) 611
SsiI CCGC 1 cut(s) 676
SspMI CTAG 5 cut(s) 66, 506, 578, 702, 902
StuI AGGCCT 1 cut(s) 611
StyD4I CCNGG 4 cut(s) 400, 826, 876, 1042
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 304, 1114
TaiI ACGT 1 cut(s) 291
TaqI TCGA 4 cut(s) 99, 162, 759, 991
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 5 cut(s) 405, 467, 512, 553, 1195
Tru1I TTAA 5 cut(s) 53, 375, 536, 801, 864
Tru9I TTAA 5 cut(s) 53, 375, 536, 801, 864
TscAI CASTG 2 cut(s) 34, 940
TseFI GTSAC 1 cut(s) 657
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 3 cut(s) 212, 914, 975
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 2 cut(s) 34, 940
Tth111I GACNNNGTC 1 cut(s) 655
Vha464I CTTAAG 2 cut(s) 374, 863
VpaK11BI GGWCC 2 cut(s) 731, 1029
VspI ATTAAT 1 cut(s) 536
XapI RAATTY 5 cut(s) 459, 766, 882, 962, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 5 cut(s) 66, 506, 578, 702, 902
Zsp2I ATGCAT 2 cut(s) 273, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.