FvH4_3g03670

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008620
Physical Location & Seq
Forward (+)
2055536 .. 2058092
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03670.t1

Sequence Viewer

Length: 762 bp
ATGGGGGATTTGGAGAAGCAGCAGCTGAACAAGGACGAGGCGGCGGCGGAGGAGACGGAGAGGCTTGTCGACGACGGAGGAATGGCCGTGTTGGATTTCGACATGCTCTGCTCCACCGTCGCCTTACAGACCCAGGGAAAGTCCGCCGCCAAGTTTCAGAGCTTCGACGGCGGAGATGAAGCTGAGGCTGCGGAATTGGGCGGCGTGTTTAGGATGTGGGAGGGTGAGCTCCTAGATTGCTTCGAAGATCGCCGCGTCGCTCTCGAATCGGCTTGCTGTCCGTGCTATACATTTGGGAAGAACATGAAGCGAGCTGGGTTTGGTCCTTGCTTTTTACAGGGTTCTCTTCATTTAATCCTTGTCGCCAGCATCCTTCTCAACTGCGTTGCGTTTATTATCACCAAGAAGCGCTGCTTTGTATATCTGGTGGTTGCTTTCACCATTACACTGGGGACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATAAGGGGTAATGAGAGTTCCGTGGATGATTGCCTCTACCACCTTGTCTGTCCATGCTGCACATTATCTCAGGAAGCCAGAACATTAGAGATGAACAATGTCCAAGATGGCACCTGGCACGGTAGGGGAGATACAATATATATAGGCAGCCTTGGAGAAGGAGGAAAATCTTTCTTTGAGCTCCAACCACCTACTCTTGTGTCAATCAAGTCTCCCGACCTCTGCAACACGCAAAAAAGTCCAGCTGCTAGTACATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

254

Amino Acids

27.82

Weight (kDa)

4.91

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 73 - 193 4.1e-22 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 608
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 255
AciI CCGC 9 cut(s) 41, 44, 47, 144, 147, 171, 191, 201, 253
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 1 cut(s) 491
AfaI GTAC 3 cut(s) 467, 475, 751
AfeI AGCGCT 1 cut(s) 410
AjnI CCWGG 2 cut(s) 132, 611
AluBI AGCT 7 cut(s) 25, 162, 182, 229, 314, 679, 743
AluI AGCT 7 cut(s) 25, 162, 182, 229, 314, 679, 743
Alw21I GWGCWC 2 cut(s) 231, 681
Alw26I GTCTC 2 cut(s) 47, 714
AlwNI CAGNNNCTG 1 cut(s) 25
Aor51HI AGCGCT 1 cut(s) 410
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 7 cut(s) 19, 22, 188, 411, 555, 645, 743
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 3 cut(s) 236, 391, 430
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 457, 490
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 2 cut(s) 231, 681
Bbv12I GWGCWC 2 cut(s) 231, 681
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 7 cut(s) 31, 34, 175, 398, 542, 657, 730
BccI CCATC 1 cut(s) 599
BceAI ACGGC 2 cut(s) 71, 184
BciT130I CCWGG 2 cut(s) 134, 613
BcoDI GTCTC 2 cut(s) 47, 714
BfaI CTAG 2 cut(s) 233, 747
BfoI RGCGCY 1 cut(s) 412
Bme1390I CCNGG 2 cut(s) 134, 613
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 2 cut(s) 134, 613
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 458
Bpu10I CCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 243
BsaJI CCNNGG 4 cut(s) 132, 133, 519, 649
Bse1I ACTGG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 134, 613
BseDI CCNNGG 4 cut(s) 132, 133, 519, 649
BseGI GGATG 3 cut(s) 219, 369, 529
BseMII CTCAG 2 cut(s) 174, 581
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 7 cut(s) 31, 34, 175, 398, 542, 657, 730
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 541
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 608
BsiHKAI GWGCWC 2 cut(s) 231, 681
BslFI GGGAC 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 47, 714
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 231, 681
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 9 cut(s) 41, 44, 47, 144, 147, 171, 191, 201, 253
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 175, 580
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 1 cut(s) 758
BspLI GGNNCC 1 cut(s) 610
BspT104I TTCGAA 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 608
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 4 cut(s) 132, 133, 519, 649
BssMI GATC 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 649
Bst2UI CCWGG 2 cut(s) 134, 613
Bst4CI ACNGT 2 cut(s) 118, 620
Bst6I CTCTTC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 3 cut(s) 274, 312, 367
BstDEI CTNAG 2 cut(s) 183, 567
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 219, 369, 529
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 2 cut(s) 47, 714
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 168, 188, 282
BstNI CCWGG 2 cut(s) 134, 613
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 132, 611
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 7 cut(s) 31, 34, 175, 398, 542, 657, 730
BstXI CCANNNNNNTGG 1 cut(s) 448
BsuRI GGCC 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 3 cut(s) 219, 369, 529
BtsIMutI CAGTG 1 cut(s) 446
Cac8I GCNNGC 3 cut(s) 274, 312, 367
CaiI CAGNNNCTG 1 cut(s) 25
CciI TCATGA 1 cut(s) 758
CfoI GCGC 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 323
CseI GACGC 1 cut(s) 244
Csp6I GTAC 3 cut(s) 466, 474, 750
CviAII CATG 4 cut(s) 103, 304, 552, 759
CviQI GTAC 3 cut(s) 466, 474, 750
DdeI CTNAG 2 cut(s) 183, 567
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 3 cut(s) 62, 133, 186
Ecl136II GAGCTC 2 cut(s) 229, 679
Eco130I CCWWGG 1 cut(s) 649
Eco24I GRGCYC 2 cut(s) 231, 681
Eco47I GGWCC 1 cut(s) 323
Eco47III AGCGCT 1 cut(s) 410
Eco53kI GAGCTC 2 cut(s) 229, 679
EcoICRI GAGCTC 2 cut(s) 229, 679
EcoRII CCWGG 2 cut(s) 132, 611
EcoT14I CCWWGG 1 cut(s) 649
EcoT38I GRGCYC 2 cut(s) 231, 681
ErhI CCWWGG 1 cut(s) 649
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 4 cut(s) 106, 307, 555, 762
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 1 cut(s) 466
FatI CATG 4 cut(s) 102, 303, 551, 758
FblI GTMKAC 1 cut(s) 69
FokI GGATG 3 cut(s) 226, 356, 536
FriOI GRGCYC 2 cut(s) 231, 681
FspBI CTAG 2 cut(s) 233, 747
GlaI GCGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 1 cut(s) 86
HgaI GACGC 1 cut(s) 244
HhaI GCGC 1 cut(s) 411
Hin1II CATG 4 cut(s) 106, 307, 555, 762
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 266
HphI GGTGA 3 cut(s) 236, 391, 430
Hpy166II GTNNAC 2 cut(s) 70, 476
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 4 cut(s) 263, 569, 713, 759
Hpy8I GTNNAC 2 cut(s) 70, 476
Hpy99I CGWCG 5 cut(s) 74, 77, 122, 170, 260
HpyAV CCTTC 2 cut(s) 383, 650
HpyCH4III ACNGT 2 cut(s) 118, 620
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 2 cut(s) 558, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 188, 282
HpyF3I CTNAG 2 cut(s) 183, 567
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 4 cut(s) 106, 307, 555, 762
HspAI GCGC 1 cut(s) 409
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 3 cut(s) 116, 234, 684
Lsp1109I GCAGC 7 cut(s) 31, 34, 175, 398, 542, 657, 730
LweI GCATC 1 cut(s) 378
MaeI CTAG 2 cut(s) 233, 747
MaeII ACGT 1 cut(s) 455
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 3 cut(s) 257, 310, 338
MhlI GDGCHC 2 cut(s) 231, 681
MluCI AATT 2 cut(s) 194, 491
MmeI TCCRAC 2 cut(s) 72, 706
MnlI CCTC 9 cut(s) 31, 43, 54, 71, 178, 214, 542, 653, 728
MseI TTAA 2 cut(s) 353, 495
MslI CAYNNNNRTG 1 cut(s) 757
MspA1I CMGCKG 2 cut(s) 25, 743
MspR9I CCNGG 2 cut(s) 134, 613
MvaI CCWGG 2 cut(s) 134, 613
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 168, 188, 282
NdeII GATC 1 cut(s) 247
NlaIII CATG 4 cut(s) 106, 307, 555, 762
NlaIV GGNNCC 1 cut(s) 610
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 243
PagI TCATGA 1 cut(s) 758
PasI CCCWGGG 1 cut(s) 133
PfeI GAWTC 1 cut(s) 266
Psp124BI GAGCTC 2 cut(s) 231, 681
Psp6I CCWGG 2 cut(s) 132, 611
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 2 cut(s) 132, 611
PspN4I GGNNCC 1 cut(s) 610
PspPI GGNCC 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstNI CAGNNNCTG 1 cut(s) 25
PvuII CAGCTG 2 cut(s) 25, 743
RsaI GTAC 3 cut(s) 467, 475, 751
RsaNI GTAC 3 cut(s) 466, 474, 750
RseI CAYNNNNRTG 1 cut(s) 757
SacI GAGCTC 2 cut(s) 231, 681
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 2 cut(s) 353, 495
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 134, 613
SduI GDGCHC 2 cut(s) 231, 681
SfaNI GCATC 1 cut(s) 378
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 757
Sse9I AATT 2 cut(s) 194, 491
SsiI CCGC 9 cut(s) 41, 44, 47, 144, 147, 171, 191, 201, 253
SspMI CTAG 2 cut(s) 233, 747
SstI GAGCTC 2 cut(s) 231, 681
StyD4I CCNGG 2 cut(s) 132, 611
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 2 cut(s) 118, 620
TaiI ACGT 1 cut(s) 458
TaqI TCGA 5 cut(s) 69, 99, 165, 243, 264
TasI AATT 2 cut(s) 194, 491
TatI WGTACW 1 cut(s) 749
TauI GCSGC 5 cut(s) 44, 47, 149, 204, 255
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 2 cut(s) 353, 495
Tru9I TTAA 2 cut(s) 353, 495
TscAI CASTG 1 cut(s) 453
TseI GCWGC 7 cut(s) 19, 22, 188, 411, 555, 645, 743
TspDTI ATGAA 5 cut(s) 192, 320, 338, 605, 747
TspGWI ACGGA 5 cut(s) 71, 90, 270, 461, 508
TspRI CASTG 1 cut(s) 453
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 491
XceI RCATGY 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 2 cut(s) 233, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.