RchiOBHm_Chr5g0005711

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008620
Physical Location & Seq
Forward (+)
3633898 .. 3636423
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28691

Sequence Viewer

Length: 780 bp
ATGGGGGATTTAGAGAAGCAAGCAGAGAGGGTTGTGCTGAGCAAGGACGAGGCGGAAGAGGAGGAGATGGAGAGGCTGGTCGGCGGCGGAATGGCGGTGTTGGATTTCGACATGCTCTGCTCCACCGTCGCCTTACAGACCCAGGGCAAGTCCGCCGCCAAGTTCCAGAGCTTTGGGGCCGGAGAAGAAGAAGAAGAAGCTGAGGTTGGGGATTTCGGCGGCGTGTTCCGGATGTGGGAGGGTGAGCTTCTCGATTGGTTCGACGATCGCCGCGTCGCTATTCAGTCGGCTTGCTGTCCGTGCTATACATTTGGGAAGAACATGATGCGAGCTGGTTTTGGTCCTGGCTTTTTACAGGGAACTCTTCATTTAGTTCTTGTTGCGAGCATCCTTCTCAACTGCATTGCGTTTATTATCACCAAGAAGCGCTGCTTTATATATCTGGTGGTTGCTTTCACCATTACACTAGGAACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATACGGGGTAATGATAGTTCCATGGATGATTGCATCTACCACCTTGTCTGCCCATGCTGCACGTTATCTCAGGAAGCCAGAACATTAGAGATGAACAATGTCCAAGATGGCACCTGGCATGGCAGGGGCGATTCAATATACATAGGCAGCCTTGGAGAAGGAGGAAAGTCTTTCTTTGAGCTCCAAGCACCTCCTCTTGTGTCAATCAAGTCCCCCGACCTCTGCAACGCGCAAAAAAGTACAGCGACTAGTGCATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

259

Amino Acids

28.59

Weight (kDa)

4.81

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 79 - 199 7.2e-20 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 626
AccII CGCG 2 cut(s) 273, 746
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 8 cut(s) 53, 84, 87, 95, 153, 156, 219, 271
AcsI RAATTY 1 cut(s) 509
AfaI GTAC 3 cut(s) 485, 493, 757
AfeI AGCGCT 1 cut(s) 428
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 1 cut(s) 651
AhlI ACTAGT 1 cut(s) 764
AjnI CCWGG 3 cut(s) 141, 343, 629
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 5 cut(s) 171, 200, 247, 332, 697
AluI AGCT 5 cut(s) 171, 200, 247, 332, 697
Alw21I GWGCWC 1 cut(s) 699
Aor13HI TCCGGA 1 cut(s) 228
Aor51HI AGCGCT 1 cut(s) 428
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 3 cut(s) 429, 573, 663
ApoI RAATTY 1 cut(s) 509
AspLEI GCGC 2 cut(s) 429, 748
AspS9I GGNCC 2 cut(s) 177, 341
AsuHPI GGTGA 3 cut(s) 254, 409, 448
AvaII GGWCC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 475, 508
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 699
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 3 cut(s) 416, 560, 675
BccI CCATC 2 cut(s) 61, 617
BciT130I CCWGG 3 cut(s) 143, 345, 631
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 2 cut(s) 467, 765
BfoI RGCGCY 1 cut(s) 430
BisI GCNGC 7 cut(s) 85, 156, 220, 271, 430, 574, 664
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 7 cut(s) 86, 157, 221, 272, 431, 575, 665
Bme1390I CCNGG 3 cut(s) 143, 345, 631
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 177, 341
BmiI GGNNCC 2 cut(s) 178, 628
BmrFI CCNGG 3 cut(s) 143, 345, 631
BmsI GCATC 3 cut(s) 315, 396, 558
Bpu10I CCTNAGC 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 141, 142, 537, 667
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 3 cut(s) 143, 345, 631
BseDI CCNNGG 4 cut(s) 141, 142, 537, 667
BseGI GGATG 3 cut(s) 237, 387, 547
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 3 cut(s) 29, 192, 599
BseRI GAGGAG 3 cut(s) 74, 77, 699
BseXI GCAGC 3 cut(s) 416, 560, 675
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 273, 746
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 699
BsiSI CCGG 2 cut(s) 180, 229
BslFI GGGAC 1 cut(s) 712
BslI CCNNNNNNNGG 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 712
BsnI GGCC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 1 cut(s) 265
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 8 cut(s) 53, 84, 87, 95, 153, 156, 219, 271
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 3 cut(s) 30, 193, 598
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 2 cut(s) 273, 746
BspHI TCATGA 1 cut(s) 776
BspLI GGNNCC 2 cut(s) 178, 628
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 141, 142, 537, 667
BssMI GATC 1 cut(s) 265
BssT1I CCWWGG 2 cut(s) 537, 667
Bst2UI CCWGG 3 cut(s) 143, 345, 631
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 2 cut(s) 51, 369
BstC8I GCNNGC 4 cut(s) 21, 292, 330, 385
BstDEI CTNAG 3 cut(s) 38, 201, 585
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 3 cut(s) 237, 387, 547
BstFNI CGCG 2 cut(s) 273, 746
BstH2I RGCGCY 1 cut(s) 430
BstHHI GCGC 2 cut(s) 429, 748
BstKTI GATC 1 cut(s) 268
BstMBI GATC 1 cut(s) 265
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 3 cut(s) 300, 573, 767
BstNI CCWGG 3 cut(s) 143, 345, 631
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 141, 343, 629
BstUI CGCG 2 cut(s) 273, 746
BstV1I GCAGC 3 cut(s) 416, 560, 675
BstXI CCANNNNNNTGG 1 cut(s) 173
BsuRI GGCC 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 3 cut(s) 237, 387, 547
Cac8I GCNNGC 4 cut(s) 21, 292, 330, 385
CciI TCATGA 1 cut(s) 776
CfoI GCGC 2 cut(s) 429, 748
Cfr13I GGNCC 2 cut(s) 177, 341
CseI GACGC 1 cut(s) 262
Csp6I GTAC 3 cut(s) 484, 492, 756
CviAII CATG 6 cut(s) 112, 322, 538, 570, 635, 777
CviQI GTAC 3 cut(s) 484, 492, 756
DdeI CTNAG 3 cut(s) 38, 201, 585
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 51, 369
EarI CTCTTC 2 cut(s) 51, 369
EciI GGCGGA 3 cut(s) 68, 102, 142
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 2 cut(s) 537, 667
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 341
Eco47III AGCGCT 1 cut(s) 428
Eco53kI GAGCTC 1 cut(s) 697
EcoICRI GAGCTC 1 cut(s) 697
EcoRII CCWGG 3 cut(s) 141, 343, 629
EcoT14I CCWWGG 2 cut(s) 537, 667
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 2 cut(s) 537, 667
FaeI CATG 6 cut(s) 115, 325, 541, 573, 638, 780
FalI AAGNNNNNCTT 4 cut(s) 416, 448, 674, 706
FaqI GGGAC 1 cut(s) 712
FatI CATG 6 cut(s) 111, 321, 537, 569, 634, 776
Fnu4HI GCNGC 7 cut(s) 85, 156, 220, 271, 430, 574, 664
FokI GGATG 3 cut(s) 244, 374, 554
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 7 cut(s) 85, 156, 220, 271, 430, 574, 664
FspBI CTAG 2 cut(s) 467, 765
GlaI GCGC 2 cut(s) 428, 747
GluI GCNGC 7 cut(s) 85, 156, 220, 271, 430, 574, 664
HaeII RGCGCY 1 cut(s) 430
HaeIII GGCC 1 cut(s) 179
HapII CCGG 2 cut(s) 180, 229
HgaI GACGC 1 cut(s) 262
HhaI GCGC 2 cut(s) 429, 748
Hin1II CATG 6 cut(s) 115, 325, 541, 573, 638, 780
Hin6I GCGC 2 cut(s) 427, 746
HinP1I GCGC 2 cut(s) 427, 746
HinfI GANTC 1 cut(s) 647
HpaII CCGG 2 cut(s) 180, 229
HphI GGTGA 3 cut(s) 254, 409, 448
Hpy166II GTNNAC 1 cut(s) 494
Hpy188III TCNNGA 5 cut(s) 166, 229, 251, 587, 777
Hpy8I GTNNAC 1 cut(s) 494
Hpy99I CGWCG 3 cut(s) 131, 266, 278
HpyAV CCTTC 2 cut(s) 401, 668
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 2 cut(s) 473, 578
HpyCH4V TGCA 5 cut(s) 402, 549, 576, 741, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 300, 573, 767
HpyF3I CTNAG 3 cut(s) 38, 201, 585
HpySE526I ACGT 2 cut(s) 473, 578
Hsp92II CATG 6 cut(s) 115, 325, 541, 573, 638, 780
HspAI GCGC 2 cut(s) 427, 746
Kpn2I TCCGGA 1 cut(s) 228
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 2 cut(s) 125, 702
Lsp1109I GCAGC 3 cut(s) 416, 560, 675
LweI GCATC 3 cut(s) 315, 396, 558
MaeI CTAG 2 cut(s) 467, 765
MaeII ACGT 2 cut(s) 473, 578
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 7 cut(s) 68, 197, 200, 203, 206, 328, 356
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 81
MroI TCCGGA 1 cut(s) 228
MseI TTAA 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 775
MspI CCGG 2 cut(s) 180, 229
MspR9I CCNGG 3 cut(s) 143, 345, 631
MvaI CCWGG 3 cut(s) 143, 345, 631
MvnI CGCG 2 cut(s) 273, 746
MwoI GCNNNNNNNGC 3 cut(s) 300, 573, 767
NcoI CCATGG 1 cut(s) 537
NdeII GATC 1 cut(s) 265
NlaIII CATG 6 cut(s) 115, 325, 541, 573, 638, 780
NlaIV GGNNCC 2 cut(s) 178, 628
NspI RCATGY 1 cut(s) 115
PagI TCATGA 1 cut(s) 776
PasI CCCWGGG 1 cut(s) 142
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 270
PfeI GAWTC 1 cut(s) 647
PkrI GCNGC 7 cut(s) 86, 157, 221, 272, 431, 575, 665
Ple19I CGATCG 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 699
Psp6I CCWGG 3 cut(s) 141, 343, 629
PspGI CCWGG 3 cut(s) 141, 343, 629
PspN4I GGNNCC 2 cut(s) 178, 628
PspPI GGNCC 2 cut(s) 177, 341
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PvuI CGATCG 1 cut(s) 268
RsaI GTAC 3 cut(s) 485, 493, 757
RsaNI GTAC 3 cut(s) 484, 492, 756
RseI CAYNNNNRTG 1 cut(s) 775
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 7 cut(s) 85, 156, 220, 271, 430, 574, 664
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 2 cut(s) 177, 341
ScrFI CCNGG 3 cut(s) 143, 345, 631
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 3 cut(s) 315, 396, 558
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 775
SpeI ACTAGT 1 cut(s) 764
Sse9I AATT 1 cut(s) 509
SsiI CCGC 8 cut(s) 53, 84, 87, 95, 153, 156, 219, 271
SspMI CTAG 2 cut(s) 467, 765
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 141, 343, 629
StyI CCWWGG 2 cut(s) 537, 667
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 2 cut(s) 476, 581
TaqI TCGA 3 cut(s) 108, 252, 261
TasI AATT 1 cut(s) 509
TatI WGTACW 1 cut(s) 755
TauI GCSGC 4 cut(s) 87, 158, 222, 273
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TseI GCWGC 3 cut(s) 429, 573, 663
TspDTI ATGAA 3 cut(s) 356, 623, 765
TspGWI ACGGA 2 cut(s) 288, 479
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 115
XspI CTAG 2 cut(s) 467, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.