MD10G1305800.v1.1

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008620
Physical Location & Seq
Reverse (-)
39177397 .. 39179854
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1305800.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGGGGATTTGGAGAAGCAAGAGACGGTGCTACTGAAGAAGGCAGCGGAGGAGGCGGAGGGGGAAGAAAAGGAGAGGCTTTTGGAGGGAAATGGAATGGCGGTCGTCGATTTCGACCTGCTCTGCTCGACGGTGGCGCTGCAGACCCAGGGAAAGCTGGCGGCCAAGCTCCAGAGCTTCCCCGACGTAGAGGAAGGCGGAGACGCAGGGGACTTGGGTGGCGTCTTCAGGATGTGGGAAGGTGAAGTCCTAGATTGCTTCGATGACCGTCGTGTTGCGCTCGAATCCCTCTGTTGCCCATGCTATAGATTTGGGAAGAACATGAGACGAGCTGGTTTCGGTCCTTGTTCGTTACAGGGAGCTCTTCATTTGATTCTTGTTGTTCTTGTCCTTCTCAACTGCATTGCCTTCATTGTCACCAAGAAGCACTGCTTTCTATATTTGGCGGTTGCTTTCACCATTTCACTAGGAACGTATTTGGGGTACTTCCGAACACAGATGAAGAAGAAATTTAACATCAGGGGTGGTGATAGTTCCTTGGATGATTGCATCTACCATCTCATCTGCCCATGCTGTGCATTATGTCAGGAATCCAGAACATTGGAGATGAACAATGTCCAAGATGGTACCTGGCATGGTCGGGGTGATACGATTTACATAGGTAGCCTTGGAGAAGGTGCAAAATCGTTCTTTGAGCTACAACCGCCTCCGATTGTCTCAATCAAATCCCCTGACCCCTGCAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

250

Amino Acids

27.4

Weight (kDa)

5.11

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 79 - 199 1.6e-21 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 126
Acc65I GGTACC 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 626
AciI CCGC 7 cut(s) 47, 56, 101, 161, 198, 446, 704
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 2 cut(s) 56, 211
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 485, 628
AjnI CCWGG 2 cut(s) 147, 629
AluBI AGCT 7 cut(s) 157, 169, 177, 332, 362, 697, 744
AluI AGCT 7 cut(s) 157, 169, 177, 332, 362, 697, 744
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 4 cut(s) 17, 195, 319, 721
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 44, 139, 741
ApoI RAATTY 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 626
AspLEI GCGC 2 cut(s) 139, 280
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 5 cut(s) 254, 409, 448, 539, 656
AvaII GGWCC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 2 cut(s) 56, 126
BccI CCATC 2 cut(s) 564, 617
BciT130I CCWGG 2 cut(s) 149, 631
BcoDI GTCTC 4 cut(s) 17, 195, 319, 721
BfaI CTAG 2 cut(s) 251, 467
BfmI CTRYAG 3 cut(s) 140, 304, 739
BfoI RGCGCY 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 126
BisI GCNGC 4 cut(s) 45, 140, 162, 742
BlsI GCNGC 4 cut(s) 46, 141, 163, 743
Bme1390I CCNGG 2 cut(s) 149, 631
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 2 cut(s) 149, 631
BmsI GCATC 1 cut(s) 558
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 147, 148, 537, 667
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 2 cut(s) 149, 631
BseDI CCNNGG 4 cut(s) 147, 148, 537, 667
BseGI GGATG 2 cut(s) 237, 547
BseMI GCAATG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 56, 126
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 364
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 4 cut(s) 17, 195, 319, 721
BsmBI CGTCTC 3 cut(s) 17, 195, 319
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 364
BspACI CCGC 7 cut(s) 47, 56, 101, 161, 198, 446, 704
BspANI GGCC 1 cut(s) 164
BspLI GGNNCC 1 cut(s) 628
BspMAI CTGCAG 2 cut(s) 144, 743
BspMI ACCTGC 1 cut(s) 126
BspQI GCTCTTC 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 147, 148, 537, 667
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 537, 667
Bst2UI CCWGG 2 cut(s) 149, 631
Bst4CI ACNGT 3 cut(s) 28, 133, 269
Bst6I CTCTTC 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 159
BstF5I GGATG 2 cut(s) 237, 547
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 2 cut(s) 139, 280
BstMAI GTCTC 4 cut(s) 17, 195, 319, 721
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 53, 703
BstNI CCWGG 2 cut(s) 149, 631
BstSCI CCNGG 2 cut(s) 147, 629
BstSFI CTRYAG 3 cut(s) 140, 304, 739
BstV1I GCAGC 2 cut(s) 56, 126
BstV2I GAAGAC 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 601
BsuRI GGCC 1 cut(s) 164
BtsCI GGATG 2 cut(s) 237, 547
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 427
BveI ACCTGC 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 2 cut(s) 139, 280
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 2 cut(s) 211, 212
Csp6I GTAC 2 cut(s) 484, 627
CviAII CATG 4 cut(s) 300, 322, 570, 635
CviQI GTAC 2 cut(s) 484, 627
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EciI GGCGGA 2 cut(s) 71, 213
Ecl136II GAGCTC 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 537, 667
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 56, 211
EcoICRI GAGCTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 147, 629
EcoT14I CCWWGG 2 cut(s) 537, 667
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 537, 667
Esp3I CGTCTC 3 cut(s) 17, 195, 319
FaeI CATG 4 cut(s) 303, 325, 573, 638
FaiI YATR 8 cut(s) 301, 306, 323, 439, 571, 583, 636, 659
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 299, 321, 569, 634
Fnu4HI GCNGC 4 cut(s) 45, 140, 162, 742
FokI GGATG 2 cut(s) 244, 554
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 4 cut(s) 45, 140, 162, 742
FspBI CTAG 2 cut(s) 251, 467
GlaI GCGC 2 cut(s) 138, 279
GluI GCNGC 4 cut(s) 45, 140, 162, 742
GsuI CTGGAG 1 cut(s) 155
HaeII RGCGCY 1 cut(s) 140
HaeIII GGCC 1 cut(s) 164
HgaI GACGC 2 cut(s) 211, 212
HhaI GCGC 2 cut(s) 139, 280
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 303, 325, 573, 638
Hin6I GCGC 2 cut(s) 137, 278
HinP1I GCGC 2 cut(s) 137, 278
HinfI GANTC 3 cut(s) 284, 373, 590
HphI GGTGA 5 cut(s) 254, 409, 448, 539, 656
Hpy188I TCNGA 2 cut(s) 491, 711
Hpy188III TCNNGA 4 cut(s) 172, 229, 587, 594
Hpy99I CGWCG 4 cut(s) 110, 133, 188, 273
HpyAV CCTTC 6 cut(s) 34, 188, 233, 401, 418, 668
HpyCH4III ACNGT 3 cut(s) 28, 133, 269
HpyCH4IV ACGT 2 cut(s) 186, 473
HpyCH4V TGCA 6 cut(s) 142, 402, 549, 578, 680, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 703
HpySE526I ACGT 2 cut(s) 186, 473
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 303, 325, 573, 638
HspAI GCGC 2 cut(s) 137, 278
KpnI GGTACC 1 cut(s) 630
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 2 cut(s) 174, 359
Lsp1109I GCAGC 2 cut(s) 56, 126
LweI GCATC 1 cut(s) 558
MaeI CTAG 2 cut(s) 251, 467
MaeII ACGT 2 cut(s) 186, 473
MaeIII GTNAC 2 cut(s) 351, 415
MboII GAAGA 7 cut(s) 49, 77, 217, 328, 356, 514, 517
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 1 cut(s) 509
MnlI CCTC 8 cut(s) 43, 46, 52, 69, 79, 184, 299, 717
MseI TTAA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 149, 631
MvaI CCWGG 2 cut(s) 149, 631
MwoI GCNNNNNNNGC 2 cut(s) 53, 703
NlaIII CATG 4 cut(s) 303, 325, 573, 638
NlaIV GGNNCC 1 cut(s) 628
NmuCI GTSAC 1 cut(s) 415
PasI CCCWGGG 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 3 cut(s) 284, 373, 590
PkrI GCNGC 4 cut(s) 46, 141, 163, 743
Psp124BI GAGCTC 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 147, 629
PspGI CCWGG 2 cut(s) 147, 629
PspN4I GGNNCC 1 cut(s) 628
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 2 cut(s) 144, 743
RsaI GTAC 2 cut(s) 485, 628
RsaNI GTAC 2 cut(s) 484, 627
SacI GAGCTC 1 cut(s) 364
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 45, 140, 162, 742
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 2 cut(s) 149, 631
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 1 cut(s) 558
SfcI CTRYAG 3 cut(s) 140, 304, 739
SinI GGWCC 1 cut(s) 341
Sse9I AATT 1 cut(s) 509
SsiI CCGC 7 cut(s) 47, 56, 101, 161, 198, 446, 704
SspMI CTAG 2 cut(s) 251, 467
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 147, 629
StyI CCWWGG 2 cut(s) 537, 667
TaaI ACNGT 3 cut(s) 28, 133, 269
TaiI ACGT 2 cut(s) 189, 476
TaqI TCGA 5 cut(s) 108, 114, 128, 261, 282
TaqII GACCGA 1 cut(s) 329
TasI AATT 1 cut(s) 509
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 284, 373, 590
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 44, 139, 741
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 356, 400, 515, 623
TspRI CASTG 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 509
XspI CTAG 2 cut(s) 251, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.